LOCUS       pLSU-1//t35S[TMV]:mNG-I 6099 bp ds-DNA     circular     18-SEP-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      1..195
                     /label="VS1 ORI"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          226..243
                     /label="pLSU-1 5' F"
                     /note="sequence: gcaaaaagcgcctaccct"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    362..385
                     /label="T-DNA LB Repeat"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          388..415
                     /label="315bp t35S F"
                     /note="sequence: ATATTGTCGCATGCGGAAACCTCCTCGGATTC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          390..415
                     /label="t35S SphI F"
                     /note="sequence: ATATTGTCGCATGCGGAAACCTCCTCGGATTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          398..415
                     /label="HindIII t35s fwd"
                     /note="sequence: GGAAGAGGAAGCTTGGAAACCTCCTCGGATTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    398..712
                     /label="315bp t35S"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          559..578
                     /label="LBS41 (35S_SF1)"
                     /note="sequence: GAGGAGCATCGTGGAAAAAG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          597..624
                     /label="35sF"
                     /note="sequence: CTTCAAAGCAAGTGGATTGATGTGATAT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          642..662
                     /label="Kanamycin Sequence Primer Reverse"
                     /note="sequence: ATGACGCACAATCCCACTATC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          683..736
                     /label="Ovr-t35s-UTR-F"
                     /note="sequence: ATATAAGGAAGTTCATTTCATTTGGAGAGGACACGCTCGAGTATAAGAGCTCAT"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(690..718)
                     /label="315bp t35S R"
                     /note="sequence: GACAATATCTCGAGCCTCTCCAAATGAAATGAACTTC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(692..712)
                     /label="t35s_R"
                     /note="sequence: CCTCTCCAAATGAAATGAACT"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(693..733)
                     /label="pLSU-1//t35s:MCS:NosT___Linear REV"
                     /note="sequence: GCTCTTATACTCGAGCGTGTGCCTCTCCAAATGAAATGAAC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    713..805
                     /label="TMV 5' UTR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="TMV Leader Sequence https://doi.org/10.1016/j.bbrc.2010.11.102 "
     primer          complement(775..804)
                     /label="LBS17 (35S 5' Rev Bracket)"
                     /note="sequence: GTATCGATAATTGTAAATGTAATTGTAATG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(775..805)
                     /label="TMV_MluI_R"
                     /note="sequence: GAGCACGCGTGTATCGATAATTGTAAATGTAATTGTAATG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(775..826)
                     /label="Ovr-t35s-UTR-R"
                     /note="sequence: ATCCGAATTCGGTACCCTCGATGTATCGATAATTGTAAATGTAATTGTAATG"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(806..827)
                     /label="MCS_BstZ17I_R"
                     /note="sequence: tgacCTGCAGGTATACGATCCGAATTCGGTACCCTCGA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    823..1722
                     /label="mNG-I (PDK intron)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(1001..1020)
                     /label="mNG07 (mNG 5' Rev Bracket)"
                     /note="sequence: GTGAGGGACTAGGATCCATG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          1722..1751
                     /label="NosT F"
                     /note="sequence: ATATTGTCCTGCAGGATCGTTCAAACATTTGGCAATA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          1728..1747
                     /label="ffLUZ-NosT-f"
                     /note="sequence: GGGGGAAGAGTAAGTTGTAACCCGGGGATCGTTCAAACATTTGGC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     regulatory      1729..1984
                     /label="NOS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1730..1981
                     /label="Nos Terminator"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(1905..1925)
                     /label="NosT-r_br"
                     /note="sequence: attttgttttctatcgcgtat"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(1959..1990)
                     /label="NosT R"
                     /note="sequence: GACAATATGAGCTCCCCGATCTAGTAACATAGATGACACC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1991..2052
                     /label="T-DNA RB Repeat + Overdrive"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2058..2167
                     /label="Tn903 nptI Promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(2133..2158)
                     /label="pLSU1_3'_R"
                     /note="sequence: TGTATTACTGTTTATGTAAGCAGACA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2133..2158)
                     /label="pLSU1_3R"
                     /note="sequence: TGTATTACTGTTTATGTAAGCAGACA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2133..2158)
                     /label="pLSU-1 3' R"
                     /note="sequence: tgtattactgtttatgtaagcagaca"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(2133..2158)
                     /label="pLSU-1 3' Rev"
                     /note="sequence: tgtattactgtttatgtaagcagaca"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(2133..2158)
                     /label="pLSU 3R"
                     /note="sequence: TGTATTACTGTTTATGTAAGCAGACA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2143..2167)
                     /label="pNPTI R"
                     /note="sequence: AACACCCCTTGTATTACTGTTTATG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2146..2167)
                     /label="Seq8_R"
                     /note="sequence: AACACCCCTTGTATTACTGTTT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2155..2174
                     /label="C-1F"
                     /note="sequence: TACAAGGGGTGTTATGAGCC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2168..2188
                     /label="NPT1"
                     /note="sequence: ATGAGCCATATTCAACGGGAA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     CDS             2168..2983
                     /label="Tn903 nptI CDS"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(2175..2194)
                     /label="BP NptI R"
                     /note="sequence: AGACGTTTCCCGTTGAATAT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    2984..3069
                     /label="Tn903 nptI Terminator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     rep_origin      3101..3657
                     /label="ColE1 ORI"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_binding    3730..3752
                     /label="misc_binding"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             3903..4532
                     /label="staA CDS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     gene            4859..6034
                     /label="repA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
ORIGIN
        1 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
       61 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
      121 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
      181 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
      241 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
      301 ccgctcaaaa atggctggcc tacggccagg caatctgcta gcgcgcggac aagctaggat
      361 tggcaggata tattcagttg taaatGATAT CGCATGCGGA AACCTCCTCG GATTCcattg
      421 cccagctatc tgtcacttta ttgagaagat agtggaaaag gaaggtggct cctacaaatg
      481 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
      541 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      601 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
      661 tccttcgcaa gacccttcct ctatataagG AAGTTCATTT CATTTGGAGA GGACACGCTC
      721 GAGTATAAGA GCTCATTTTT ACAACAATTA CCAACAACAA CAAACAACAA ACAACATTAC
      781 AATTACATTT ACAATTATCG ATACATCGAG GGTACCGAAT TCATGGTTTC CAAAGGTGAG
      841 GAAGATAATA TGGCTAGTTT ACCTGCCACA CATGAGCTAC ACATATTCGG CAGCATAAAC
      901 GGTGTAGATT TTGATATGGT TGGACAAGGG ACAGGTAATC CTAATGATGG ATATGAGGAG
      961 TTGAACCTCA AATCTACCAA GGGGGACCTT CAATTCTCCC CATGGATCCT AGTCCCTCAC
     1021 gtaaatttct agtttttctc cttcattttc ttggttagga cccttttctc tttttatttt
     1081 tttgagcttt gatctttctt taaactgatc tattttttaa ttgattggtt atggtgtaaa
     1141 tattacatag ctttaactga taatctgatt actttatttc gtgtgtctat gatgatgatg
     1201 atgttacagA TTGGATACGG CTTCCACCAG TACTTACCAT ACCCAGATGG AATGTCCCCT
     1261 TTCCAAGCAG CCATGGTTGA CGGTTCCGGG TACCAGGTAC ATAGGACAAT GCAATTTGAG
     1321 GACGGGGCTT CTCTGACTGT GAATTACCGA TATACCTATG AAGGCagTCA CATAAAGGGC
     1381 GAAGCACAAG TGAAGGGCAC CGGATTTCCT GCCGACGGGC CTGTAATGAC TAATAGCTTG
     1441 ACCGCCGCAG ATTGGTGCAG GTCAAAAAAG ACCTACCCAA ATGACAAGAC AATAATCTCT
     1501 ACATTTAAGT GGAGCTACAC TACAGGGAAT GGTAAACGAT ATAGATCAAC TGCTAGAACC
     1561 ACATATACCT TCGCTAAACC TATGGCAGCC AATTATCTGA AGAACCAGCC CATGTATGTG
     1621 TTCCGAAAGA CTGAACTCAA GCATAGCAAA ACCGAACTCA ACTTCAAAGA GTGGCAAAAG
     1681 GCTTTCACTG ACGTGATGGG AATGGACGAG TTATATAAGT AACTGCAGGA TCGTTCAAAC
     1741 ATTTGGCAAT Aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata
     1801 taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt
     1861 atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac
     1921 aaaatatagc gcgcaaacta ggataaatta tcgcgcgcGG TGTCATCTAT GTTACTAGAT
     1981 CGGGGagctc tggcaggata tatgcggttg taattcattt ttattgtcta aatttctgta
     2041 tttgtttgtt tgcaattaga tccacgttgt gtctcaaaat ctctgatgtt acattgcaca
     2101 agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag
     2161 gggtgttatg agccatattc aacgggaaac gtcttgttcg aggccgcgat taaattccaa
     2221 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     2281 gacaatctac cgattgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     2341 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     2401 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     2461 cactgcgatc ccagggaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     2521 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     2581 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     2641 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     2701 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     2761 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     2821 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     2881 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     2941 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
     3001 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcggctttg ttgaatcaat
     3061 tgatcttctt gaaatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3121 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3181 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     3241 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3301 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3361 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3421 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     3481 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     3541 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     3601 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     3661 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     3721 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgatctgat
     3781 cctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg
     3841 cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac
     3901 gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa
     3961 cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc
     4021 cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg
     4081 gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag
     4141 tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact
     4201 tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg
     4261 agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt
     4321 cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg
     4381 agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg
     4441 ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc
     4501 tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa
     4561 aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt
     4621 ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga
     4681 tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc
     4741 tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt
     4801 tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat
     4861 gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt gtctgccggc
     4921 cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgagcggtc gcaaaccatc
     4981 cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggccgc
     5041 gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc
     5101 ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat
     5161 taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt
     5221 gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga
     5281 ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc
     5341 agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg cggtttccca
     5401 tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt
     5461 ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa
     5521 agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac
     5581 gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg
     5641 ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagttga
     5701 ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga
     5761 ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc
     5821 aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg
     5881 agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga
     5941 gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa
     6001 cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat
     6061 tgccctagca ggggaaaaag gtcgaaaagg tctctttcc
//