LOCUS       pLSU-1//t35S[TMV]:mNG-C 5995 bp ds-DNA     circular     07-NOV-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      1..195
                     /label="VS1 ORI"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          226..243
                     /label="pLSU-1 5' F"
                     /note="sequence: gcaaaaagcgcctaccct"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    362..385
                     /label="T-DNA LB Repeat"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          388..415
                     /label="315bp t35S F"
                     /note="sequence: ATATTGTCGCATGCGGAAACCTCCTCGGATTC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          390..415
                     /label="t35S SphI F"
                     /note="sequence: ATATTGTCGCATGCGGAAACCTCCTCGGATTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          398..415
                     /label="HindIII t35s fwd"
                     /note="sequence: GGAAGAGGAAGCTTGGAAACCTCCTCGGATTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    398..712
                     /label="315bp t35S"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          559..578
                     /label="LBS41 (35S_SF1)"
                     /note="sequence: GAGGAGCATCGTGGAAAAAG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          597..624
                     /label="35sF"
                     /note="sequence: CTTCAAAGCAAGTGGATTGATGTGATAT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          642..662
                     /label="Kanamycin Sequence Primer Reverse"
                     /note="sequence: ATGACGCACAATCCCACTATC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          683..736
                     /label="Ovr-t35s-UTR-F"
                     /note="sequence: ATATAAGGAAGTTCATTTCATTTGGAGAGGACACGCTCGAGTATAAGAGCTCAT"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(690..718)
                     /label="315bp t35S R"
                     /note="sequence: GACAATATCTCGAGCCTCTCCAAATGAAATGAACTTC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(692..712)
                     /label="t35s_R"
                     /note="sequence: CCTCTCCAAATGAAATGAACT"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(693..733)
                     /label="pLSU-1//t35s:MCS:NosT___Linear REV"
                     /note="sequence: GCTCTTATACTCGAGCGTGTGCCTCTCCAAATGAAATGAAC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    713..805
                     /label="TMV 5' UTR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="TMV Leader Sequence https://doi.org/10.1016/j.bbrc.2010.11.102 "
     primer          complement(775..804)
                     /label="LBS17 (35S 5' Rev Bracket)"
                     /note="sequence: GTATCGATAATTGTAAATGTAATTGTAATG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(775..805)
                     /label="TMV_MluI_R"
                     /note="sequence: GAGCACGCGTGTATCGATAATTGTAAATGTAATTGTAATG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(775..826)
                     /label="Ovr-t35s-UTR-R"
                     /note="sequence: ATCCGAATTCGGTACCCTCGATGTATCGATAATTGTAAATGTAATTGTAATG"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(806..827)
                     /label="MCS_BstZ17I_R"
                     /note="sequence: tgacCTGCAGGTATACGATCCGAATTCGGTACCCTCGA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    823..1618
                     /label="mNG-CI (AGO)"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    1262..1263
                     /label="Correction"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          1618..1647
                     /label="NosT F"
                     /note="sequence: ATATTGTCCTGCAGGATCGTTCAAACATTTGGCAATA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          1624..1643
                     /label="ffLUZ-NosT-f"
                     /note="sequence: GGGGGAAGAGTAAGTTGTAACCCGGGGATCGTTCAAACATTTGGC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     regulatory      1625..1880
                     /label="NOS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1626..1877
                     /label="Nos Terminator"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(1801..1821)
                     /label="NosT-r_br"
                     /note="sequence: attttgttttctatcgcgtat"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(1855..1886)
                     /label="NosT R"
                     /note="sequence: GACAATATGAGCTCCCCGATCTAGTAACATAGATGACACC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1887..1948
                     /label="T-DNA RB Repeat + Overdrive"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1954..2063
                     /label="Tn903 nptI Promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(2029..2054)
                     /label="pLSU1_3R"
                     /note="sequence: TGTATTACTGTTTATGTAAGCAGACA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2029..2054)
                     /label="pLSU 3R"
                     /note="sequence: TGTATTACTGTTTATGTAAGCAGACA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2029..2054)
                     /label="pLSU-1 3' R"
                     /note="sequence: tgtattactgtttatgtaagcagaca"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(2029..2054)
                     /label="pLSU1_3'_R"
                     /note="sequence: TGTATTACTGTTTATGTAAGCAGACA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2029..2054)
                     /label="pLSU-1 3' Rev"
                     /note="sequence: tgtattactgtttatgtaagcagaca"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(2039..2063)
                     /label="pNPTI R"
                     /note="sequence: AACACCCCTTGTATTACTGTTTATG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2042..2063)
                     /label="Seq8_R"
                     /note="sequence: AACACCCCTTGTATTACTGTTT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2051..2070
                     /label="C-1F"
                     /note="sequence: TACAAGGGGTGTTATGAGCC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2064..2084
                     /label="NPT1"
                     /note="sequence: ATGAGCCATATTCAACGGGAA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     CDS             2064..2879
                     /label="Tn903 nptI CDS"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(2071..2090)
                     /label="BP NptI R"
                     /note="sequence: AGACGTTTCCCGTTGAATAT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    2880..2965
                     /label="Tn903 nptI Terminator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     rep_origin      2997..3553
                     /label="ColE1 ORI"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_binding    3626..3648
                     /label="misc_binding"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             3799..4428
                     /label="staA CDS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     gene            4755..5930
                     /label="repA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
ORIGIN
        1 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
       61 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
      121 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
      181 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
      241 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
      301 ccgctcaaaa atggctggcc tacggccagg caatctgcta gcgcgcggac aagctaggat
      361 tggcaggata tattcagttg taaatGATAT CGCATGCGGA AACCTCCTCG GATTCcattg
      421 cccagctatc tgtcacttta ttgagaagat agtggaaaag gaaggtggct cctacaaatg
      481 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
      541 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      601 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
      661 tccttcgcaa gacccttcct ctatataagG AAGTTCATTT CATTTGGAGA GGACACGCTC
      721 GAGTATAAGA GCTCATTTTT ACAACAATTA CCAACAACAA CAAACAACAA ACAACATTAC
      781 AATTACATTT ACAATTATCG ATACATCGAG GGTACCGAAT TCATGGTTTC CAAAGGTGAG
      841 GAAGATAATA TGGCTAGTTT ACCTGCCACA CATGAGCTAC ACATATTCGG CAGCATAAAC
      901 GGTGTAGATT TTGATATGGT TGGACAAGGG ACAGGTAATC CTAATGATGG ATATGAGGAG
      961 TTGAACCTCA AATCTACCAA GGGGGACCTT CAATTCTCCC CATGGATCCT AGTCCCTCAC
     1021 gtaagtttca tctatcaact caaaacttta tttgttaatg tttgctcctc tgaatcgttt
     1081 taatactctt tatgttctga attagATTGG ATACGGCTTC CACCAGTACT TACCATACCC
     1141 AGATGGAATG TCCCCTTTCC AAGCAGCCAT GGTTGACGGT TCCGGGTACC AGGTACATAG
     1201 GACAATGCAA TTTGAGGACG GGGCTTCTCT GACTGTGAAT TACCGATATA CCTATGAAGG
     1261 CTCTCACATA AAGGGCGAAG CACAAGTGAA GGGCACCGGA TTTCCTGCCG ACGGGCCTGT
     1321 AATGACTAAT AGCTTGACCG CCGCAGATTG GTGCAGGTCA AAAAAGACCT ACCCAAATGA
     1381 CAAGACAATA ATCTCTACAT TTAAGTGGAG CTACACTACA GGGAATGGTA AACGATATAG
     1441 ATCAACTGCT AGAACCACAT ATACCTTCGC TAAACCTATG GCAGCCAATT ATCTGAAGAA
     1501 CCAGCCCATG TATGTGTTCC GAAAGACTGA ACTCAAGCAT AGCAAAACCG AACTCAACTT
     1561 CAAAGAGTGG CAAAAGGCTT TCACTGACGT GATGGGAATG GACGAGTTAT ATAAGTAACT
     1621 GCAGGATCGT TCAAACATTT GGCAATAaag tttcttaaga ttgaatcctg ttgccggtct
     1681 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
     1741 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
     1801 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcGGTGTC
     1861 ATCTATGTTA CTAGATCGGG Gagctctggc aggatatatg cggttgtaat tcatttttat
     1921 tgtctaaatt tctgtatttg tttgtttgca attagatcca cgttgtgtct caaaatctct
     1981 gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca
     2041 taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct tgttcgaggc
     2101 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg
     2161 tcgggcaatc aggtgcgaca atctaccgat tgtatgggaa gcccgatgcg ccagagttgt
     2221 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa
     2281 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg
     2341 atgcatggtt actcaccact gcgatcccag ggaaaacagc attccaggta ttagaagaat
     2401 atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt
     2461 cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc
     2521 aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct
     2581 ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag
     2641 tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag
     2701 gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat
     2761 ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta
     2821 ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat
     2881 cagaattggt taattggttg taacactggc agagcattac gctgacttga cgggacggcg
     2941 gctttgttga atcaattgat cttcttgaaa tccttttttt ctgcgcgtaa tctgctgctt
     3001 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     3061 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     3121 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     3181 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     3241 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     3301 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     3361 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     3421 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     3481 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     3541 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     3601 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     3661 ctttgagtga tctgatcctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt
     3721 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca
     3781 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc
     3841 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg
     3901 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa
     3961 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc
     4021 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg
     4081 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc
     4141 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa
     4201 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag
     4261 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac
     4321 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc
     4381 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta
     4441 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca
     4501 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc
     4561 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca
     4621 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa
     4681 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc
     4741 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc
     4801 tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga
     4861 gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg
     4921 agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg
     4981 gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag
     5041 ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc
     5101 cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc
     5161 gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc
     5221 acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac
     5281 tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca
     5341 agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg
     5401 atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg
     5461 ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg
     5521 aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg
     5581 agatcgagct agttgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc
     5641 tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc
     5701 tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac
     5761 gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt
     5821 caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag
     5881 tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc
     5941 agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcc
//