LOCUS       pLSU-1//t35S[]:mCherry: 5779 bp ds-DNA     circular     07-NOV-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      1..195
                     /label="VS1 ORI"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          226..243
                     /label="pLSU-1 5' F"
                     /note="sequence: gcaaaaagcgcctaccct"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    362..385
                     /label="T-DNA LB Repeat"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          388..415
                     /label="315bp t35S F"
                     /note="sequence: ATATTGTCGCATGCGGAAACCTCCTCGGATTC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          390..415
                     /label="t35S SphI F"
                     /note="sequence: ATATTGTCGCATGCGGAAACCTCCTCGGATTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          398..415
                     /label="HindIII t35s fwd"
                     /note="sequence: GGAAGAGGAAGCTTGGAAACCTCCTCGGATTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    398..712
                     /label="315bp t35S"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          559..578
                     /label="LBS41 (35S_SF1)"
                     /note="sequence: GAGGAGCATCGTGGAAAAAG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          597..624
                     /label="35sF"
                     /note="sequence: CTTCAAAGCAAGTGGATTGATGTGATAT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          642..662
                     /label="Kanamycin Sequence Primer Reverse"
                     /note="sequence: ATGACGCACAATCCCACTATC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(690..718)
                     /label="315bp t35S R"
                     /note="sequence: GACAATATCTCGAGCCTCTCCAAATGAAATGAACTTC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(692..712)
                     /label="t35s_R"
                     /note="sequence: CCTCTCCAAATGAAATGAACT"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(693..733)
                     /label="pLSU-1//t35s:MCS:NosT___Linear REV"
                     /note="sequence: GCTCTTATACTCGAGCGTGTGCCTCTCCAAATGAAATGAAC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          722..739
                     /label="mCherry Rev"
                     /note="sequence: ATATCTCGAGATGGCCATCATCAAGGAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          722..739
                     /label="mCherry Normal Rev"
                     /note="sequence: ATGGCCATCATCAAGGAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    722..1396
                     /label="X-Gluchy mCherry_sequence confirmed"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             722..1396
                     /label="Translation 722-1396"
                     /translation="MAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDE*"
     primer          882..910
                     /label="mCherryBrack F"
                     /note="sequence: GGGACATCCTGAGCCCTCAGTTCATGTAC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(884..905)
                     /label="mCherry_I_R2"
                     /note="sequence: tcaaaggtagaagcagaaacttacCTGGGGGCTCAGGATGTC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    899..901
                     /label="Chromophore AA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          899..921
                     /label="mCherry_I_F2"
                     /note="sequence: gaccaaaatttgttgatgtgcagTTCATGTACGGCAGCAAGGC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(1015..1039)
                     /label="mCherryBrack R"
                     /note="sequence: GTCCTGGGTCACGGTCACCACGCCG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(1370..1393)
                     /label="nmCherryR"
                     /note="sequence: GGGGACGCGTCTCGTCCATGCCGCCGGTGCTGTG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          1402..1431
                     /label="NosT F"
                     /note="sequence: ATATTGTCCTGCAGGATCGTTCAAACATTTGGCAATA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     regulatory      1409..1664
                     /label="NOS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1410..1661
                     /label="Nos Terminator"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(1585..1605)
                     /label="NosT-r_br"
                     /note="sequence: attttgttttctatcgcgtat"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(1639..1670)
                     /label="NosT R"
                     /note="sequence: GACAATATGAGCTCCCCGATCTAGTAACATAGATGACACC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(1644..1671)
                     /label="NosT-r (SacI)"
                     /note="sequence: AAAGAGCTCCCCGATCTAGTAACATAGATG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1671..1732
                     /label="T-DNA RB Repeat + Overdrive"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1738..1847
                     /label="Tn903 nptI Promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(1813..1838)
                     /label="pLSU-1 3' R"
                     /note="sequence: tgtattactgtttatgtaagcagaca"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(1826..1847)
                     /label="Seq8_R"
                     /note="sequence: AACACCCCTTGTATTACTGTTT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     CDS             1848..2663
                     /label="Tn903 nptI CDS"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2664..2749
                     /label="Tn903 nptI Terminator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     rep_origin      2781..3337
                     /label="ColE1 ORI"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_binding    3410..3432
                     /label="misc_binding"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             3583..4212
                     /label="staA CDS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     gene            4539..5714
                     /label="repA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
ORIGIN
        1 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
       61 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
      121 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
      181 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
      241 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
      301 ccgctcaaaa atggctggcc tacggccagg caatctgcta gcgcgcggac aagctaggat
      361 tggcaggata tattcagttg taaatGATAT CGCATGCGGA AACCTCCTCG GATTCcattg
      421 cccagctatc tgtcacttta ttgagaagat agtggaaaag gaaggtggct cctacaaatg
      481 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
      541 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      601 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
      661 tccttcgcaa gacccttcct ctatataagG AAGTTCATTT CATTTGGAGA GGCCTACGCG
      721 TATGGCCATC ATCAAGGAGT TCATGCGGTT CAAGGTGCAC ATGGAAGGCA GCGTGAACGG
      781 CCACGAGTTC GAGATCGAGG GCGAGGGCGA AGGCAGGCCC TACGAGGGCA CCCAGACCGC
      841 CAAGCTGAAG GTGACCAAGG GCGGACCTCT GCCCTTCGCC TGGGACATCC TGAGCCCCCA
      901 GTTCATGTAC GGCAGCAAGG CCTACGTGAA GCACCCCGCC GACATCCCCG ACTACCTGAA
      961 GCTGTCCTTC CCCGAGGGAT TCAAATGGGA GCGGGTGATG AACTTCGAGG ACGGCGGCGT
     1021 GGTGACCGTG ACCCAGGACA GCAGCCTGCA GGACGGCGAG TTCATCTACA AGGTGAAACT
     1081 GCGGGGCACC AACTTCCCCA GCGACGGCCC CGTGATGCAG AAAAAGACCA TGGGCTGGGA
     1141 GGCCAGCAGC GAGCGGATGT ACCCCGAGGA TGGCGCCCTG AAGGGCGAGA TCAAGCAGCG
     1201 GCTGAAGCTG AAGGATGGCG GCCACTACGA CGCCGAGGTG AAAACCACCT ACAAGGCCAA
     1261 GAAACCCGTG CAGCTGCCTG GCGCCTACAA CGTGAACATC AAGCTGGACA TCACCAGCCA
     1321 CAACGAGGAC TACACCATCG TGGAGCAGTA CGAGCGGGCC GAGGGCAGAC ACAGCACCGG
     1381 CGGCATGGAC GAGTAAGTAT ACCTGCAGGA TCGTTCAAAC ATTTGGCAAT Aaagtttctt
     1441 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
     1501 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
     1561 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
     1621 ggataaatta tcgcgcgcGG TGTCATCTAT GTTACTAGAT CGGGGagctc tggcaggata
     1681 tatgcggttg taattcattt ttattgtcta aatttctgta tttgtttgtt tgcaattaga
     1741 tccacgttgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     1801 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc
     1861 aacgggaaac gtcttgttcg aggccgcgat taaattccaa catggatgct gatttatatg
     1921 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctac cgattgtatg
     1981 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg
     2041 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca
     2101 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc ccagggaaaa
     2161 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg
     2221 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc
     2281 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg
     2341 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac
     2401 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta
     2461 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc
     2521 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga
     2581 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt
     2641 tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca
     2701 ttacgctgac ttgacgggac ggcggctttg ttgaatcaat tgatcttctt gaaatccttt
     2761 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     2821 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     2881 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     2941 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     3001 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     3061 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     3121 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     3181 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     3241 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     3301 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     3361 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     3421 ttctgtggat aaccgtatta ccgcctttga gtgatctgat cctgaagaaa ccgagcgccg
     3481 ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta
     3541 tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta
     3601 cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg
     3661 caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat
     3721 tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt
     3781 gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag
     3841 gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag
     3901 ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt
     3961 gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg
     4021 cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc
     4081 cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa
     4141 tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca
     4201 aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg
     4261 ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc
     4321 cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt
     4381 acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac
     4441 cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc
     4501 atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg
     4561 ggcggttggc caggcgtaag cggctgggtt gtctgccggc cctgcaatgg cactggaacc
     4621 cccaagcccg aggaatcggc gtgagcggtc gcaaaccatc cggcccggta caaatcggcg
     4681 cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac
     4741 gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca
     4801 aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg
     4861 acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca
     4921 gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga
     4981 tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca
     5041 gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc
     5101 gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg
     5161 tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct
     5221 gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc
     5281 gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg
     5341 aaaccgggcg gccggagtac atcgagatcg agctagttga ttggatgtac cgcgagatca
     5401 cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg
     5461 gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat
     5521 ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt
     5581 tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg
     5641 cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat
     5701 ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag
     5761 gtcgaaaagg tctctttcc
//