LOCUS       pLSU-1//t35S[TMV]:nLuc: 5709 bp ds-DNA     circular     10-APR-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_binding    606..628
                     /label="misc_binding"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             779..1408
                     /label="staA CDS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     gene            1735..2910
                     /label="repA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     rep_origin      2976..3170
                     /label="VS1 ORI"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          3201..3218
                     /label="pLSU-1 5' F"
                     /note="sequence: gcaaaaagcgcctaccct"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    3337..3360
                     /label="T-DNA LB Repeat"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          3363..3390
                     /label="315bp t35S F"
                     /note="sequence: ATATTGTCGCATGCGGAAACCTCCTCGGATTC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          3365..3390
                     /label="t35S SphI F"
                     /note="sequence: ATATTGTCGCATGCGGAAACCTCCTCGGATTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          3365..3392
                     /label="t35sP Forward-SphI"
                     /note="sequence: TTTCGCATGCGGAAACCTCCTCGGATTCca"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    3373..3687
                     /label="315bp t35S"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          3534..3553
                     /label="LBS41 (35S_SF1)"
                     /note="sequence: GAGGAGCATCGTGGAAAAAG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          3572..3599
                     /label="35sF"
                     /note="sequence: CTTCAAAGCAAGTGGATTGATGTGATAT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          3617..3637
                     /label="Kanamycin Sequence Primer Reverse"
                     /note="sequence: ATGACGCACAATCCCACTATC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(3647..3687)
                     /label="t35s_StuI_MluI_R"
                     /note="sequence: tacgACGCGTAGGCCTCTCCAAATGAAATGAACTTCcttatatagaggaagggt"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          3658..3711
                     /label="Ovr-t35s-UTR-F"
                     /note="sequence: ATATAAGGAAGTTCATTTCATTTGGAGAGGACACGCTCGAGTATAAGAGCTCAT"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(3665..3687)
                     /label="t35s-35s5'UTR-fusion-R"
                     /note="sequence: ATGAGCTCTTATACTCGAGCGTGTGCCTCTCCAAATGAAATGAACTTC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(3665..3688)
                     /label="LBS50(v2)"
                     /note="sequence: GCAGAACTTGTATCCTGTACCATCCTCTCCAAATGAAATGAACTTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(3665..3693)
                     /label="315bp t35S R"
                     /note="sequence: GACAATATCTCGAGCCTCTCCAAATGAAATGAACTTC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(3667..3687)
                     /label="t35s_R"
                     /note="sequence: CCTCTCCAAATGAAATGAACT"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(3668..3708)
                     /label="pLSU-1//t35s:MCS:NosT___Linear REV"
                     /note="sequence: GCTCTTATACTCGAGCGTGTGCCTCTCCAAATGAAATGAAC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3688..3780
                     /label="TMV 5' UTR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="TMV Leader Sequence https://doi.org/10.1016/j.bbrc.2010.11.102 "
     primer          complement(3750..3779)
                     /label="LBS17 (35S 5' Rev Bracket)"
                     /note="sequence: GTATCGATAATTGTAAATGTAATTGTAATG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(3750..3780)
                     /label="TMV_MluI_R"
                     /note="sequence: GAGCACGCGTGTATCGATAATTGTAAATGTAATTGTAATG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          3780..3807
                     /label="MluI_nLuc_F"
                     /note="sequence: tgcaACGCGTATGGTGTTTACTCTTGAAGATT"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          3785..3804
                     /label="nLUC1"
                     /note="sequence: TAATATGGTGTTTACTCTTGAAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(3785..3805)
                     /label="pLSU2-r_br"
                     /note="sequence: TCTTCAAGAGTAAACACCATa"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          3786..3803
                     /label="nLuc-I_AscI_F"
                     /note="sequence: ctgaGGCGCGCCATGGTGTTTACTCTTGAA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          3786..3804
                     /label="NLucBCL"
                     /note="sequence: TATTATTGATCAATGGTGTTTACTCTTGAAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          3786..3807
                     /label="MluI_nLuc_F"
                     /note="sequence: tgcaACGCGTATGGTGTTTACTCTTGAAGATT"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          3786..3810
                     /label="MVN:NanoLuc Fusion Fwd"
                     /note="sequence: CTGGAAATTGGAATGGTGTTTACTCTTGAAGATTTCG"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          3786..3810
                     /label="NLF2"
                     /note="sequence: ATGGTGTTTACTCTTGAAGATTTCG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    3786..4301
                     /label="NLuc"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             3786..4301
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRIVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVTPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWRLCERILA*"
     primer          3787..3806
                     /label="nLUC-f_SpeI"
                     /note="sequence: AAAAACTAGTTGGTGTTTACTCTTGAAGAT"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(3959..3979)
                     /label="nLUC-r_br"
                     /note="sequence: TCGTATGGGATGATCACATGG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(4047..4062)
                     /label="NLpdk2"
                     /note="sequence: CTAGAAATTTACCTTAAAGTGGTG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(4048..4062)
                     /label="NLpt2"
                     /note="sequence: GGTAGAAGCAGAAACTTACCTTAAAGTGGT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          4057..4074
                     /label="NLpt5"
                     /note="sequence: TTGTTGATGTGCAGGTAATACTTCACTACG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          4105..4125
                     /label="nLUC-f_br"
                     /note="sequence: ATATGATCGACTACTTCGGCC"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(4269..4302)
                     /label="Nanoluc-KpnI-R"
                     /note="sequence: TGAACGATCGGTACCTTAAGCTAATATACGCTCGCACAGACGCCACCC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(4275..4300)
                     /label="MVN:NanoLuc 3' BamHI Rvs"
                     /note="sequence: GCCGGATCCCTAAGCTAATATACGCTCGCACAGACG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(4278..4301)
                     /label="NLR2"
                     /note="sequence: TTAAGCTAATATACGCTCGCACAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(4281..4301)
                     /label="nLUC-r_XmaI"
                     /note="sequence: AAAACCCGGGTTAAGCTAATATACGCTCGCA"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(4281..4301)
                     /label="ToMoV:mLIR:nLUC-r"
                     /note="sequence: AAAAtctagaTTAAGCTAATATACGCTCGCA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(4281..4301)
                     /label="mLIR:nLUC-r"
                     /note="sequence: ACGTACGTACGAGCTCTTAAGCTAATATACGCTCGCA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(4281..4302)
                     /label="mLIR:nLUC-r"
                     /note="sequence: ACGTACGTACGAGCTCTTAAGCTAATATACGCTCGCA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(4281..4302)
                     /label="mLIR:nLUC-r"
                     /note="sequence: ACGTACGTACGAGCTCTTAAGCTAATATACGCTCGCA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(4282..4308)
                     /label="BstZ17I_nLuc_R"
                     /note="sequence: atcgGTATACTTAAGCTAATATACGCTCGC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(4283..4301)
                     /label="nLUC2"
                     /note="sequence: AGATTTAAGCTAATATACGCTCG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(4283..4302)
                     /label="nLuc-I_NotI_R"
                     /note="sequence: agctGCGGCCGCTTAAGCTAATATACGCTCG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(4288..4302)
                     /label="NlucBamHI"
                     /note="sequence: TCTATGGATCCTTAAGCTAATATAC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          4307..4336
                     /label="NosT F"
                     /note="sequence: ATATTGTCCTGCAGGATCGTTCAAACATTTGGCAATA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     regulatory      4314..4569
                     /label="NOS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    4315..4566
                     /label="Nos Terminator"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(4490..4510)
                     /label="NosT-r_br"
                     /note="sequence: attttgttttctatcgcgtat"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(4542..4566)
                     /label="LBS12"
                     /note="sequence: AAAATCTAGAGATCTAGTAACATAGATGACACCGC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(4543..4566)
                     /label="NosT R"
                     /note="sequence: GATCTAGTAACATAGATGACACCg"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(4543..4566)
                     /label="mNG(nosT) reverse sacI"
                     /note="sequence: GATCTAGTAACATAGATGACACCg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(4543..4566)
                     /label="fusion mNG(nosT) reverse sacI"
                     /note="sequence: GATCTAGTAACATAGATGACACCg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(4543..4575)
                     /label="NosT_R_SacI"
                     /note="sequence: tactgagctCCCCGATCTAGTAACATAGATGACACCg"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(4544..4575)
                     /label="NosT R"
                     /note="sequence: GACAATATGAGCTCCCCGATCTAGTAACATAGATGACACC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(4549..4576)
                     /label="NosT-r (SacI)"
                     /note="sequence: AAAGAGCTCCCCGATCTAGTAACATAGATG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    4576..4637
                     /label="T-DNA RB Repeat + Overdrive"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(4577..4595)
                     /label="TI RB Rev"
                     /note="sequence: CAACCGCATATATCCTGCC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(4620..4642)
                     /label="pLSU-1_MneonGree REV"
                     /note="sequence: GGAGTTGGTCTCGacaattgcaaacaaacaaatacaga"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    4643..4752
                     /label="Tn903 nptI Promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          4647..4668
                     /label="pNPTI F"
                     /note="sequence: CCACGTTGTGTCTCAAAATCTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(4718..4739)
                     /label="Reverse Primer"
                     /note="sequence: ttactgtttatgtaagcagaca"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(4718..4743)
                     /label="pLSU-1 3' R"
                     /note="sequence: tgtattactgtttatgtaagcagaca"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(4718..4743)
                     /label="pLSU 3R"
                     /note="sequence: TGTATTACTGTTTATGTAAGCAGACA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(4731..4752)
                     /label="Seq8_R"
                     /note="sequence: aacaccccttgtattactgttt"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             4753..5568
                     /label="Tn903 nptI CDS"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(4760..4779)
                     /label="BP NptI R"
                     /note="sequence: AGACGTTTCCCGTTGAATAT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    5569..5654
                     /label="Tn903 nptI Terminator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     rep_origin      5686..533
                     /label="ColE1 ORI"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
       61 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
      121 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
      181 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
      241 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
      301 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
      361 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
      421 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
      481 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
      541 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
      601 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga tctgatcctg
      661 aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc
      721 atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat
      781 gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca
      841 tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca
      901 gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat
      961 cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat
     1021 cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt
     1081 gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct
     1141 ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg
     1201 ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct
     1261 gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg
     1321 cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc
     1381 cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc
     1441 acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc
     1501 agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac
     1561 accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa
     1621 tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc
     1681 ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc
     1741 catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct gccggccctg
     1801 caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc
     1861 ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag
     1921 gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc
     1981 gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg
     2041 aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc
     2101 acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga
     2161 cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg
     2221 ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta
     2281 accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt
     2341 ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac
     2401 gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag
     2461 aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac
     2521 aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct agttgattgg
     2581 atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac
     2641 tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc
     2701 aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag
     2761 ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac
     2821 gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg
     2881 atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc
     2941 ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta cattgggaac
     3001 ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg
     3061 aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa
     3121 aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc
     3181 cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc
     3241 cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg
     3301 ccaggcaatc tgctagcgcg cggacaagct aggattggca ggatatattc agttgtaaat
     3361 GATATCGCAT GCGGAAACCT CCTCGGATTC cattgcccag ctatctgtca ctttattgag
     3421 aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc
     3481 atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
     3541 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc
     3601 tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata
     3661 taagGAAGTT CATTTCATTT GGAGAGGACA CGCTCGAGTA TAAGAGCTCA TTTTTACAAC
     3721 AATTACCAAC AACAACAAAC AACAAACAAC ATTACAATTA CATTTACAAT TATCGATACA
     3781 CGCGTATGGT GTTTACTCTT GAAGATTTCG TGGGAGACTG GCGTCAAACA GCAGGCTATA
     3841 ACTTAGACCA GGTCCTAGAA CAGGGAGGCG TTTCCAGTCT GTTTCAGAAT CTTGGAGTTA
     3901 GTGTTACACC TATCCAAAGA ATAGTTCTCA GCGGTGAAAA TGGCCTCAAA ATAGACATCC
     3961 ATGTGATCAT CCCATACGAG GGCTTGTCCG GCGATCAAAT GGGCCAGATA GAGAAAATAT
     4021 TCAAAGTAGT GTACCCAGTA GATGACCACC ACTTTAAGGT AATACTTCAC TACGGGACCT
     4081 TAGTTATCGA TGGGGTAACC CCTAATATGA TCGACTACTT CGGCCGTCCC TACGAGGGCA
     4141 TTGCTGTCTT CGATGGGAAG AAGATAACAG TTACCGGCAC TTTGTGGAAC GGTAACAAAA
     4201 TCATCGATGA GCGACTGATC AACCCAGATG GTAGCCTTCT GTTCAGGGTT ACCATTAACG
     4261 GGGTTACAGG GTGGCGTCTG TGCGAGCGTA TATTAGCTTA AGTATACCTG CAGGATCGTT
     4321 CAAACATTTG GCAATAaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta
     4381 tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt
     4441 tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag
     4501 aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcGGTGTCA TCTATGTTAC
     4561 TAGATCGGGG agctctggca ggatatatgc ggttgtaatt catttttatt gtctaaattt
     4621 ctgtatttgt ttgtttgcaa ttagatccac gttgtgtctc aaaatctctg atgttacatt
     4681 gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat
     4741 acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gttcgaggcc gcgattaaat
     4801 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     4861 ggtgcgacaa tctaccgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     4921 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     4981 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     5041 ctcaccactg cgatcccagg gaaaacagca ttccaggtat tagaagaata tcctgattca
     5101 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     5161 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     5221 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     5281 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
     5341 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     5401 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     5461 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     5521 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt
     5581 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgg ctttgttgaa
     5641 tcaattgatc ttcttgaaat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5701 aaccaccgc
//