LOCUS       pLSU-1//t35S[TMV]:nnLuz 5917 bp ds-DNA     circular     08-NOV-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      1..195
                     /label="VS1 ORI"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          226..243
                     /label="pLSU-1 5' F"
                     /note="sequence: gcaaaaagcgcctaccct"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    362..385
                     /label="T-DNA LB Repeat"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          388..415
                     /label="315bp t35S F"
                     /note="sequence: ATATTGTCGCATGCGGAAACCTCCTCGGATTC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          390..415
                     /label="t35S SphI F"
                     /note="sequence: ATATTGTCGCATGCGGAAACCTCCTCGGATTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    398..713
                     /label="315bp t35S"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          559..578
                     /label="LBS41 (35S_SF1)"
                     /note="sequence: GAGGAGCATCGTGGAAAAAG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          597..624
                     /label="35sF"
                     /note="sequence: CTTCAAAGCAAGTGGATTGATGTGATAT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(690..718)
                     /label="315bp t35S R"
                     /note="sequence: GACAATATCTCGAGCCTCTCCAAATGAAATGAACTTC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(690..737)
                     /label="t35s-35s5'UTR-fusion-R"
                     /note="sequence: ATGAGCTCTTATACTCGAGCGTGTGCCTCTCCAAATGAAATGAACTTC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          725..749
                     /label="mLuz-I-f_KpnI"
                     /note="sequence: AAAAGGTACCatgAGGATAAATATCTCTTTGTCTA"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    725..1534
                     /label="Luz"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             725..1534
                     /label="Translation 725-1534"
                     /translation="MRINISLSSLFERLSKLSSRSIAITCGVVLASAIAFPIIRRDYQTFLEVGPSYAPQNFRGYIIVCVLSLFRQEQKGLAIYDRLPEKRRWLADLPFREGTRPSITSHIIQRQRTQLVDQEFATRELIDKVIPRVQARHTDKTFLSTSKFEFHAKAIFLLPSIPINDPLNIPSHDTVRRTKREIAHMHDYHDCTLHLALAAQDGKEVLKKGWGQRHPLAGPGVPGPPTEWTFLYAPRNEEEARVVEMIVEASIGYMTNDPAGKIVENAKSG*"
     misc_feature    896..937
                     /label="Buried Alpha-Helix (PredictProtein)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    901..920
                     /label="A.t. Branch Site (Putative)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(901..921)
                     /label="mLUZ-r_br"
                     /note="sequence: ACACAAACGATGATATAGCCT"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    927..946
                     /label="A.t. Acceptor Splice Site (Putative)"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    929..994
                     /label="Conserved AAs (w/ Armillaria)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    938..1000
                     /label="Solvent-Exposed Region (PredictProtein)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    1145..1164
                     /label="A. thaliana Donor Splice Site (Putative)"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          1338..1358
                     /label="mLUZ-f_br"
                     /note="sequence: TTTTGAAGAAGGGGTGGGGCC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(1511..1531)
                     /label="Luz_NarIXhoI_Rev"
                     /note="sequence: AAAACTCGAGACCTGACTTGGCATTTTCGAC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(1512..1531)
                     /label="Fus Luz:T35s Rev V3"
                     /note="sequence: GTGATTTCAGCGAATTCGAGacctGACTTGGCATTTTCGA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(1512..1533)
                     /label="CRL4.1"
                     /note="sequence: TTCAGTACCAAAAGACAGGCAACCTGACTTGGCATTTTCGA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(1512..1533)
                     /label="Fus Luz:Int1 Rev V2"
                     /note="sequence: TTCAGTACCAAAAGACAGGCAacctGACTTGGCATTTTCGA"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(1514..1534)
                     /label="CRL4"
                     /note="sequence: TTCAGTACCAAAAGACAGGCATCAACCTGACTTGGCATTTTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(1514..1534)
                     /label="Fus Luz:T35s Rev"
                     /note="sequence: GTGATTTCAGCGAATTCGAGtcaacctGACTTGGCATTTTC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(1514..1534)
                     /label="CRL10.1"
                     /note="sequence: GTGATTTCAGCGAATTCGAGTCAACCTGACTTGGCATTTTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(1514..1534)
                     /label="mLuz-r"
                     /note="sequence: tgcatgACGCGTtcaacctGACTTGGCATTTTC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(1514..1534)
                     /label="nnL8 (Fusion nnLuz-pJET Rev)"
                     /note="sequence: ATTATTGTAGGAGATCTTCTAGAAAGATTCAACCTGACTTGGCATTTTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(1514..1534)
                     /label="nnL2 (Luz Rev XhoI)"
                     /note="sequence: AAAACTCGAGtcaacctGACTTGGCATTTTC"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(1514..1534)
                     /label="nnL12 (Luz Rev EcoRI)"
                     /note="sequence: AAAAAGAATTCtcaacctGACTTGGCATTTTC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(1514..1534)
                     /label="mLUZ-r_XmaI"
                     /note="sequence: AAAACCCGGGtcaacctGACTTGGCATTTTC"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(1514..1534)
                     /label="Fusion Luz-IntF2A-1 Rev"
                     /note="sequence: TTCAGTACCAAAAGACAGGCAtcaacctGACTTGGCATTTTC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(1514..1534)
                     /label="mLuz-r (MluI)"
                     /note="sequence: TGCATGACGCGTTCAACCTGACTTGGCATTTTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(1514..1535)
                     /label="nnL12 (Luz Rev EcoRI)"
                     /note="sequence: AAAAAGAATTCtcaacctGACTTGGCATTTTC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          1540..1569
                     /label="NosT F"
                     /note="sequence: ATATTGTCCTGCAGGATCGTTCAAACATTTGGCAATA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     regulatory      1547..1802
                     /label="NOS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1548..1799
                     /label="Nos Terminator"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(1723..1743)
                     /label="NosT-r_br"
                     /note="sequence: attttgttttctatcgcgtat"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(1777..1808)
                     /label="NosT R"
                     /note="sequence: GACAATATGAGCTCCCCGATCTAGTAACATAGATGACACC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1809..1870
                     /label="T-DNA RB Repeat + Overdrive"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1876..1985
                     /label="Tn903 nptI Promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(1951..1976)
                     /label="pLSU-1 3' R"
                     /note="sequence: tgtattactgtttatgtaagcagaca"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             1986..2801
                     /label="Tn903 nptI CDS"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2802..2887
                     /label="Tn903 nptI Terminator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     rep_origin      2919..3475
                     /label="ColE1 ORI"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_binding    3548..3570
                     /label="misc_binding"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             3721..4350
                     /label="staA CDS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     gene            4677..5852
                     /label="repA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
ORIGIN
        1 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
       61 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
      121 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
      181 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
      241 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
      301 ccgctcaaaa atggctggcc tacggccagg caatctgcta gcgcgcggac aagctaggat
      361 tggcaggata tattcagttg taaatGATAT CGCATGCGGA AACCTCCTCG GATTCcattg
      421 cccagctatc tgtcacttta ttgagaagat agtggaaaag gaaggtggct cctacaaatg
      481 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
      541 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      601 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
      661 tccttcgcaa gacccttcct ctatataagG AAGTTCATTT CATTTGGAGA GGCTCGAGGG
      721 TACCatgAGG ATAAATATCT CTTTGTCTAG TCTCTTTGAG AGACTGAGCA AATTGTCATC
      781 CCGATCCATC GCAATTACCT GCGGCGTAGT CTTGGCAAGT GCAATAGCAT TCCCAATTAT
      841 CCGAAGAGAC TATCAAACGT TCCTTGAAGT CGGTCCGAGC TATGCCCCAC AGAACTTCCG
      901 AGGCTATATC ATCGTTTGTG TCTTGTCACT TTTTAGGCAA GAGCAGAAAG GGTTGGCAAT
      961 CTATGACAGG CTTCCAGAAA AGAGGCGTTG GCTTGCCGAC TTGCCGTTTC GTGAAGGGAC
     1021 GAGGCCATCC ATAACCTCCC ACATTATACA GCGTCAGCGT ACTCAGCTGG TAGATCAAGA
     1081 GTTTGCAACC AGAGAACTTA TCGATAAGGT GATCCCACGT GTGCAAGCAA GACACACAGA
     1141 CAAAACTTTT CTCAGTACGT CAAAATTTGA GTTTCATGCA AAGGCCATCT TCCTTCTCCC
     1201 CTCTATCCCT ATTAATGATC CTCTTAACAT TCCCTCACAC GATACGGTAA GAAGAACCAA
     1261 GCGTGAGATC GCTCACATGC ATGATTACCA TGATTGCACT CTGCACTTGG CTCTTGCTGC
     1321 TCAGGATGGT AAGGAAGTTT TGAAGAAGGG GTGGGGCCAG CGTCACCCAC TGGCCGGACC
     1381 AGGAGTCCCA GGCCCTCCTA CTGAGTGGAC CTTCCTTTAC GCACCAAGGA ACGAAGAGGA
     1441 GGCCAGAGTT GTCGAGATGA TAGTCGAAGC TAGTATTGGC TACATGACTA ACGATCCTGC
     1501 TGGTAAGATT GTCGAAAATG CCAAGTCagg ttgaGGATCC CTGCAGGATC GTTCAAACAT
     1561 TTGGCAATAa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
     1621 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
     1681 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
     1741 aatatagcgc gcaaactagg ataaattatc gcgcgcGGTG TCATCTATGT TACTAGATCG
     1801 GGGagctctg gcaggatata tgcggttgta attcattttt attgtctaaa tttctgtatt
     1861 tgtttgtttg caattagatc cacgttgtgt ctcaaaatct ctgatgttac attgcacaag
     1921 ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg
     1981 gtgttatgag ccatattcaa cgggaaacgt cttgttcgag gccgcgatta aattccaaca
     2041 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
     2101 caatctaccg attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag
     2161 gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta
     2221 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca
     2281 ctgcgatccc agggaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
     2341 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
     2401 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg
     2461 gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct
     2521 ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt
     2581 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac
     2641 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
     2701 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
     2761 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt
     2821 tgtaacactg gcagagcatt acgctgactt gacgggacgg cggctttgtt gaatcaattg
     2881 atcttcttga aatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2941 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     3001 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     3061 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3121 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     3181 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     3241 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     3301 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     3361 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     3421 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     3481 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     3541 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gatctgatcc
     3601 tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg
     3661 tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc
     3721 atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc
     3781 catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc
     3841 cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc
     3901 atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg
     3961 atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc
     4021 gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag
     4081 ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg
     4141 cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag
     4201 ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc
     4261 ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg
     4321 gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa
     4381 gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg
     4441 ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc
     4501 acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg
     4561 aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta
     4621 gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc
     4681 cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgt ctgccggccc
     4741 tgcaatggca ctggaacccc caagcccgag gaatcggcgt gagcggtcgc aaaccatccg
     4801 gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc
     4861 aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg
     4921 ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta
     4981 ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg
     5041 gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc
     5101 gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag
     5161 ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc
     5221 taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc
     5281 gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag
     5341 acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga
     5401 agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct
     5461 acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagttgatt
     5521 ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt
     5581 actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag
     5641 gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag
     5701 agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt
     5761 acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc
     5821 tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg
     5881 ccctagcagg ggaaaaaggt cgaaaaggtc tctttcc
//