LOCUS       pLSU-1//t35S[TMV]:nnLuz 6199 bp ds-DNA     circular     02-MAY-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          260..275
                     /label="C-7F"
                     /note="sequence: CGCTGGCCGCTGAAAT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(367..381)
                     /label="C-6R"
                     /note="sequence: ACGTTGCAGCCTTGC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          475..496
                     /label="C-8F"
                     /note="sequence: AAGGCAAGACCATTACCGAGCT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     gene            621..1796
                     /label="repA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(818..833)
                     /label="C-7R"
                     /note="sequence: TGCCTCGATGCGTTGC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          1231..1247
                     /label="C-9F"
                     /note="sequence: ACGTTGCGGACGTACTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(1329..1344)
                     /label="C-8R"
                     /note="sequence: TCGTACGCTGCATGGC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(1803..1819)
                     /label="C-9R"
                     /note="sequence: TGCCCTAGCATCTGCTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     rep_origin      1862..2056
                     /label="VS1 ORI"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          2024..2043
                     /label="BP VS1 F"
                     /note="sequence: TAAAACTCTTAAAACCCGCC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2087..2104
                     /label="pLSU1_5'_F"
                     /note="sequence: GCAAAAAGCGCCTACCCT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2087..2104
                     /label="pLSU-1 5' Fwd"
                     /note="sequence: gcaaaaagcgcctaccct"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          2087..2104
                     /label="pLSU-1 5' F"
                     /note="sequence: gcaaaaagcgcctaccct"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          2087..2104
                     /label="pLSU-1 5' F"
                     /note="sequence: gcaaaaagcgcctaccct"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          2223..2241
                     /label="TI LB Fwd"
                     /note="sequence: GGCAGGATATATTCAGTTG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    2223..2246
                     /label="T-DNA LB Repeat"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(2224..2246)
                     /label="PB|pLSU-1 R"
                     /note="sequence: ATTTACAACTGAATATATCCTGC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2247..2261
                     /label="PB|pLSU-1;t35S F"
                     /note="sequence: GATATCGCATGCGGA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2249..2276
                     /label="315bp t35S F"
                     /note="sequence: ATATTGTCGCATGCGGAAACCTCCTCGGATTC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          2251..2276
                     /label="t35S SphI F"
                     /note="sequence: ATATTGTCGCATGCGGAAACCTCCTCGGATTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2259..2281
                     /label="35S-pEAQHT-F"
                     /note="sequence: GGAAACCTCCTCGGATTCCATTG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    2259..2574
                     /label="315bp t35S"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          2264..2283
                     /label="CaMV-35S-521-540-H"
                     /note="sequence: CCTCCTCGGATTCCATTGCC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2318..2341
                     /label="LBS30"
                     /note="sequence: AAAGGAAGGTGGCTCCTACAAATG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2369..2388)
                     /label="35S Seq R"
                     /note="sequence: CGGCAGAGGCATCTTCAACG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2420..2439
                     /label="LBS41"
                     /note="sequence: GAGGAGCATCGTGGAAAAAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2420..2439
                     /label="LBS41 (35S_SF1)"
                     /note="sequence: GAGGAGCATCGTGGAAAAAG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          2458..2485
                     /label="35sF"
                     /note="sequence: CTTCAAAGCAAGTGGATTGATGTGATAT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2503..2523
                     /label="Kanamycin Sequence Primer Reverse"
                     /note="sequence: ATGACGCACAATCCCACTATC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2544..2563)
                     /label="CaMV-35S-801-820-H"
                     /note="sequence: TGAAATGAACTTCCTTATAT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2551..2579)
                     /label="315bp t35S R"
                     /note="sequence: GACAATATCTCGAGCCTCTCCAAATGAAATGAACTTC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          2551..2598
                     /label="t35s-35s5'UTR-fusion-F"
                     /note="sequence: GAAGTTCATTTCATTTGGAGAGGCACACGCTCGAGTATAAGAGCTCAT"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(2551..2598)
                     /label="t35s-35s5'UTR-fusion-R"
                     /note="sequence: ATGAGCTCTTATACTCGAGCGTGTGCCTCTCCAAATGAAATGAACTTC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(2554..2594)
                     /label="pLSU-1//t35s:MCS:NosT___Linear REV"
                     /note="sequence: GCTCTTATACTCGAGCGTGTGCCTCTCCAAATGAAATGAAC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          2555..2594
                     /label="pLSU4/35S:HispS:nrIntF2A1:H3H:Tnos [3177-3299] FWD"
                     /note="sequence: TTCATTTCATTTGGAGAGGCACACGCTCGAGTATAAGAGC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2575..2667
                     /label="TMV 5' UTR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="TMV Leader Sequence https://doi.org/10.1016/j.bbrc.2010.11.102 "
     primer          complement(2637..2666)
                     /label="LBS17"
                     /note="sequence: GTATCGATAATTGTAAATGTAATTGTAATG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2637..2666)
                     /label="LBS17 (35S 5' Rev Bracket)"
                     /note="sequence: GTATCGATAATTGTAAATGTAATTGTAATG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          2679..2699
                     /label="mLuz-f (SpeI)"
                     /note="sequence: GTACGTACTAGTATGAGGATAAATATCTCTTTG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2679..2704
                     /label="nnL5 (Fusion pJET-nnLuz Fwd)"
                     /note="sequence: CTCGAGTTTTTCAGCAAGATATGAGGATAAATATCTCTTTGTCTAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2679..2704
                     /label="nnL11 (Luz Fwd XhoI)"
                     /note="sequence: AAAACTCGAGatgAGGATAAATATCTCTTTGTCTAG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          2679..2704
                     /label="nnL13"
                     /note="sequence: AAAAGGCGCCACCATGAGGATAAATATCTCTTTGTCTAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2679..2704
                     /label="nnL13 (Luz Fwd NarI Kozak Consensus)"
                     /note="sequence: AAAAggcgccACCATGAGGATAAATATCTCTTTGTCTAG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          2679..2704
                     /label="CRL1"
                     /note="sequence: CTCCTCATCTCACCGCCATGAGGATAAATATCTCTTTGTCTAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2679..2709
                     /label="nnL1 (Luz Fwd NarI)"
                     /note="sequence: AAAAGGCGCCatgAGGATAAATATCTCTTTGTCTAGTCTCT"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          2679..2709
                     /label="Luz_NarIXhoI_Fwd"
                     /note="sequence: AAAAGGCGCCATGAGGATAAATATCTCTTTGTCTAGTCTCT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    2679..3677
                     /label="Luz"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             2679..3677
                     /label="Translation 2679-3677"
                     /translation="MRINISLSSLFERLSKLSSRSIAITCGVVLASAIAFPIIRRDYQTFLEVGPSYAPQNFRGYIIVCVLSLFRQVSFCFYL*YIYNNYH*LVVI*YFKYFFQNKRM*YIAIAFL*FISVYILIYNFSNI*PKFVDVQEQKGLAIYDRLPEKRRWLADLPFREGTRPSITSHIIQRQRTQLVDQEFATRELIDKVIPRVQARHTDKTFLSTSKFEFHAKAIFLLPSIPINDPLNIPSHDTVRRTKREIAHMHDYHDCTLHLALAAQDGKEVLKKGWGQRHPLAGPGVPGPPTEWTFLYAPRNEEEARVVEMIVEASIGYMTNDPAGKIVENAKSG*"
     misc_feature    2850..2891
                     /label="Buried Alpha-Helix (PredictProtein)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          2852..2874
                     /label="nnL15 (Luz 5' Seq Fwd)"
                     /note="sequence: CCGAGGCTATATCATCGTTTGTG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          2852..2874
                     /label="nnL15"
                     /note="sequence: CCGAGGCTATATCATCGTTTGTG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2852..2874)
                     /label="nnL3 (Luz 5' Rev)"
                     /note="sequence: CACAAACGATGATATAGCCTCGG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(2852..2874)
                     /label="Luz_5'_R"
                     /note="sequence: CACAAACGATGATATAGCCTCGG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    2855..2874
                     /label="A.t. Branch Site (Putative)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          2872..2917
                     /label="nnL9 (Fusion nnLuz-PIV5' Forward)"
                     /note="sequence: GTGTCTTGTCACTTTTTAGGCAAGTAAGTTTCTGCTTCTACCTTTG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(2872..2917)
                     /label="nnL6 (Fusion nnLuz-PIV5' Rev)"
                     /note="sequence: CAAAGGTAGAAGCAGAAACTTACTTGCCTAAAAAGTGACAAGACAC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    2892..3143
                     /label="Solvent-Exposed Region (PredictProtein)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2895..3083
                     /label="Potato ST LS-1 Intron SV2"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          3061..3101
                     /label="nnL7 (Fusion PIV3'-nnLuz Fwd)"
                     /note="sequence: GACCAAAATTTGTTGATGTGCAGGAGCAGAAAGGGTTGGCA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(3061..3101)
                     /label="nnL10 (Fusion PIV3'-nnLuz Rev)"
                     /note="sequence: TGCCAACCCTTTCTGCTCCTGCACATCAACAAATTTTGGTC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          3174..3201
                     /label="nnL4 (Luz 3' Fwd)"
                     /note="sequence: ATAACCTCCCACATTATACAGCGTCAGC"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          3174..3201
                     /label="Luz_3'_F"
                     /note="sequence: ATAACCTCCCACATTATACAGCGTCAGC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(3177..3196)
                     /label="nnL16"
                     /note="sequence: CGCTGTATAATGTGGGAGGT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(3177..3196)
                     /label="nnL16 (Luz 3' Seq Rev)"
                     /note="sequence: CGCTGTATAATGTGGGAGGT"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    3288..3307
                     /label="A. thaliana Donor Splice Site (Putative)"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(3654..3674)
                     /label="Luz_NarIXhoI_Rev"
                     /note="sequence: AAAACTCGAGACCTGACTTGGCATTTTCGAC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(3655..3676)
                     /label="CRL4.1"
                     /note="sequence: TTCAGTACCAAAAGACAGGCAACCTGACTTGGCATTTTCGA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(3657..3677)
                     /label="nnL12 (Luz Rev EcoRI)"
                     /note="sequence: AAAAAGAATTCtcaacctGACTTGGCATTTTC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(3657..3677)
                     /label="nnL8 (Fusion nnLuz-pJET Rev)"
                     /note="sequence: ATTATTGTAGGAGATCTTCTAGAAAGATTCAACCTGACTTGGCATTTTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(3657..3677)
                     /label="nnL2 (Luz Rev XhoI)"
                     /note="sequence: AAAACTCGAGtcaacctGACTTGGCATTTTC"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(3657..3677)
                     /label="CRL4"
                     /note="sequence: TTCAGTACCAAAAGACAGGCATCAACCTGACTTGGCATTTTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(3657..3677)
                     /label="CRL10.1"
                     /note="sequence: GTGATTTCAGCGAATTCGAGTCAACCTGACTTGGCATTTTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(3657..3677)
                     /label="mLuz-r (MluI)"
                     /note="sequence: TGCATGACGCGTTCAACCTGACTTGGCATTTTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          3683..3712
                     /label="NosT F"
                     /note="sequence: ATATTGTCCTGCAGGATCGTTCAAACATTTGGCAATA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     regulatory      3690..3945
                     /label="NOS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    3691..3942
                     /label="Nos Terminator"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(3728..3746)
                     /label="Nos Terminator Sequencing Forward"
                     /note="sequence: AAGACCGGCAACAGGATTC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(3831..3855)
                     /label="nosTR"
                     /note="sequence: ATTGCGGGACTCTAATCATAAAAAC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(3866..3886)
                     /label="NosT-r_br"
                     /note="sequence: attttgttttctatcgcgtat"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(3919..3942)
                     /label="NosT R"
                     /note="sequence: GATCTAGTAACATAGATGACACCg"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(3920..3951)
                     /label="NosT R"
                     /note="sequence: GACAATATGAGCTCCCCGATCTAGTAACATAGATGACACC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3952..4013
                     /label="T-DNA RB Repeat + Overdrive"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(3953..3971)
                     /label="TI RB Rev"
                     /note="sequence: CAACCGCATATATCCTGCC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    4019..4128
                     /label="Tn903 nptI Promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          4023..4044
                     /label="pNPTI F"
                     /note="sequence: CCACGTTGTGTCTCAAAATCTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(4094..4119)
                     /label="pLSU1_3R"
                     /note="sequence: TGTATTACTGTTTATGTAAGCAGACA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(4094..4119)
                     /label="pLSU1_3'_R"
                     /note="sequence: TGTATTACTGTTTATGTAAGCAGACA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(4094..4119)
                     /label="pLSU 3R"
                     /note="sequence: TGTATTACTGTTTATGTAAGCAGACA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(4094..4119)
                     /label="pLSU-1 3' Rev"
                     /note="sequence: tgtattactgtttatgtaagcagaca"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(4094..4119)
                     /label="pLSU-1 3' R"
                     /note="sequence: tgtattactgtttatgtaagcagaca"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(4104..4128)
                     /label="pNPTI R"
                     /note="sequence: AACACCCCTTGTATTACTGTTTATG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(4107..4128)
                     /label="Seq8_R"
                     /note="sequence: AACACCCCTTGTATTACTGTTT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          4116..4135
                     /label="C-1F"
                     /note="sequence: TACAAGGGGTGTTATGAGCC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          4129..4149
                     /label="NPT1"
                     /note="sequence: ATGAGCCATATTCAACGGGAA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     CDS             4129..4944
                     /label="Tn903 nptI CDS"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(4136..4155)
                     /label="BP NptI R"
                     /note="sequence: AGACGTTTCCCGTTGAATAT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          4577..4592
                     /label="C-3F"
                     /note="sequence: CTCAGGCGCAATCACG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          4686..4704
                     /label="Kan B Fwd"
                     /note="sequence: GCCATTCTCACCGGATTCA"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(4687..4706)
                     /label="C-1R"
                     /note="sequence: ACTGAATCCGGTGAGAATGG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(4923..4944)
                     /label="NPT2"
                     /note="sequence: TTAGAAAAACTCATCGAGCATC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    4945..5030
                     /label="Tn903 nptI Terminator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          5047..5063
                     /label="C-4F"
                     /note="sequence: TGCGCGTAATCTGCTGC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     rep_origin      5062..5618
                     /label="ColE1 ORI"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(5146..5161)
                     /label="C-3R"
                     /note="sequence: TGCGCTCTGCTGAAGC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          5557..5574
                     /label="C-5F"
                     /note="sequence: GCCACCTCTGACTTGAGC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          5575..5596
                     /label="pICOz-ToMoVB Seq3_R"
                     /note="sequence: GTCGATTTTTGTGATGCTCGTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(5623..5638)
                     /label="C-4R"
                     /note="sequence: AAGGCCGCGTTGCTGG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_binding    5691..5713
                     /label="misc_binding"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             5864..294
                     /label="staA CDS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(6022..6040)
                     /label="Backbone_28"
                     /note="sequence: GACAACGGTTAGCGGTTGA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          6025..6041
                     /label="C-6F"
                     /note="sequence: ACCGCTAACCGTTGTCG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(6091..6110)
                     /label="C-5R"
                     /note="sequence: CGTCGATCACTACGAAGTCG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
ORIGIN
        1 tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg
       61 ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt
      121 gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg
      181 agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac
      241 gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat
      301 gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac
      361 gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca
      421 actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca
      481 agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat
      541 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa
      601 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta
      661 agcggctggg ttgtctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg
      721 gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc
      781 tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac
      841 gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc
      901 cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt
      961 tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg
     1021 ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag
     1081 acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc
     1141 tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg
     1201 gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc
     1261 gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca
     1321 cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg
     1381 agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt
     1441 acatcgagat cgagctagtt gattggatgt accgcgagat cacagaaggc aagaacccgg
     1501 acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct
     1561 accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct
     1621 acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga
     1681 tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga
     1741 tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta
     1801 cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggtctctttc
     1861 ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca
     1921 ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag
     1981 agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc
     2041 gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta
     2101 cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg
     2161 gccgctcaaa aatggctggc ctacggccag gcaatctgct agcgcgcgga caagctagga
     2221 ttggcaggat atattcagtt gtaaatGATA TCGCATGCGG AAACCTCCTC GGATTCcatt
     2281 gcccagctat ctgtcacttt attgagaaga tagtggaaaa ggaaggtggc tcctacaaat
     2341 gccatcattg cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca
     2401 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt
     2461 caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact
     2521 atccttcgca agacccttcc tctatataag GAAGTTCATT TCATTTGGAG AGGCACACGC
     2581 TCGAGTATAA GAGCTCATTT TTACAACAAT TACCAACAAC AACAAACAAC AAACAACATT
     2641 ACAATTACAT TTACAATTAT CGATACATCG AGGGTACCat gAGGATAAAT ATCTCTTTGT
     2701 CTAGTCTCTT TGAGAGACTG AGCAAATTGT CATCCCGATC CATCGCAATT ACCTGCGGCG
     2761 TAGTCTTGGC AAGTGCAATA GCATTCCCAA TTATCCGAAG AGACTATCAA ACGTTCCTTG
     2821 AAGTCGGTCC GAGCTATGCC CCACAGAACT TCCGAGGCTA TATCATCGTT TGTGTCTTGT
     2881 CACTTTTTAG GCAAgtaagt ttctgcttct acctttgata tatatataat aattatcatt
     2941 aattagtagt aatataatat ttcaaatatt tttttcaaaa taaaagaatg tagtatatag
     3001 caattgcttt tctgtagttt ataagtgtgt atattttaat ttataacttt tctaatatat
     3061 gaccaaaatt tgttgatgtg cagGAGCAGA AAGGGTTGGC AATCTATGAC AGGCTTCCAG
     3121 AAAAGAGGCG TTGGCTTGCC GACTTGCCGT TTCGTGAAGG GACGAGGCCA TCCATAACCT
     3181 CCCACATTAT ACAGCGTCAG CGTACTCAGC TGGTAGATCA AGAGTTTGCA ACCAGAGAAC
     3241 TTATCGATAA GGTGATCCCA CGTGTGCAAG CAAGACACAC AGACAAAACT TTTCTCAGTA
     3301 CGTCAAAATT TGAGTTTCAT GCAAAGGCCA TCTTCCTTCT CCCCTCTATC CCTATTAATG
     3361 ATCCTCTTAA CATTCCCTCA CACGATACGG TAAGAAGAAC CAAGCGTGAG ATCGCTCACA
     3421 TGCATGATTA CCATGATTGC ACTCTGCACT TGGCTCTTGC TGCTCAGGAT GGTAAGGAAG
     3481 TTTTGAAGAA GGGGTGGGGC CAGCGTCACC CACTGGCCGG ACCAGGAGTC CCAGGCCCTC
     3541 CTACTGAGTG GACCTTCCTT TACGCACCAA GGAACGAAGA GGAGGCCAGA GTTGTCGAGA
     3601 TGATAGTCGA AGCTAGTATT GGCTACATGA CTAACGATCC TGCTGGTAAG ATTGTCGAAA
     3661 ATGCCAAGTC aggttgaGGA TCCCTGCAGG ATCGTTCAAA CATTTGGCAA TAaagtttct
     3721 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
     3781 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
     3841 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
     3901 aggataaatt atcgcgcgcG GTGTCATCTA TGTTACTAGA TCGGGGagct ctggcaggat
     3961 atatgcggtt gtaattcatt tttattgtct aaatttctgt atttgtttgt ttgcaattag
     4021 atccacgttg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     4081 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt
     4141 caacgggaaa cgtcttgttc gaggccgcga ttaaattcca acatggatgc tgatttatat
     4201 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta ccgattgtat
     4261 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     4321 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
     4381 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat cccagggaaa
     4441 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
     4501 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat
     4561 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
     4621 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa
     4681 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
     4741 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
     4801 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     4861 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
     4921 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc
     4981 attacgctga cttgacggga cggcggcttt gttgaatcaa ttgatcttct tgaaatcctt
     5041 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5101 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5161 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5221 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     5281 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     5341 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     5401 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     5461 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     5521 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     5581 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     5641 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     5701 attctgtgga taaccgtatt accgcctttg agtgatctga tcctgaagaa accgagcgcc
     5761 gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt
     5821 atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt
     5881 acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct
     5941 gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga
     6001 ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat
     6061 tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca
     6121 ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca
     6181 gccaagccct tacgacata
//