LOCUS       pLSU-1/t35S:nnLuz-I:Tno 6107 bp ds-DNA     circular     18-JUN-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     gene            621..1796
                     /label="repA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     rep_origin      1862..2056
                     /label="VS1 ORI"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          2087..2104
                     /label="pLSU-1 5' Fwd"
                     /note="sequence: gcaaaaagcgcctaccct"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2223..2246
                     /label="T-DNA LB Repeat"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2259..2574
                     /label="t35s"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2581..3579
                     /label="nnLuz-Intron @tobacco"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(2754..2776)
                     /label="nnL3 (Luz 5' Rev)"
                     /note="sequence: CACAAACGATGATATAGCCTCGG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     intron          2797..2985
                     /label="intron"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          3076..3103
                     /label="nnL4 (Luz 3' Fwd)"
                     /note="sequence: ATAACCTCCCACATTATACAGCGTCAGC"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     regulatory      3598..3853
                     /label="NOS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    3599..3850
                     /label="Nos Terminator"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3860..3921
                     /label="T-DNA RB Repeat + Overdrive"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    3927..4036
                     /label="Tn903 nptI Promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(4002..4027)
                     /label="pLSU-1 3' Rev"
                     /note="sequence: tgtattactgtttatgtaagcagaca"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             4037..4852
                     /label="Tn903 nptI CDS"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    4853..4938
                     /label="Tn903 nptI Terminator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     rep_origin      4970..5526
                     /label="ColE1 ORI"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_binding    5599..5621
                     /label="misc_binding"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             5772..294
                     /label="staA CDS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
ORIGIN
        1 tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg
       61 ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt
      121 gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg
      181 agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac
      241 gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat
      301 gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac
      361 gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca
      421 actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca
      481 agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat
      541 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa
      601 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta
      661 agcggctggg ttgtctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg
      721 gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc
      781 tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac
      841 gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc
      901 cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt
      961 tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg
     1021 ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag
     1081 acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc
     1141 tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg
     1201 gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc
     1261 gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca
     1321 cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg
     1381 agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt
     1441 acatcgagat cgagctagtt gattggatgt accgcgagat cacagaaggc aagaacccgg
     1501 acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct
     1561 accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct
     1621 acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga
     1681 tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga
     1741 tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta
     1801 cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggtctctttc
     1861 ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca
     1921 ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag
     1981 agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc
     2041 gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta
     2101 cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg
     2161 gccgctcaaa aatggctggc ctacggccag gcaatctgct agcgcgcgga caagctagga
     2221 ttggcaggat atattcagtt gtaaatGATA TCGCATGCGG AAACCTCCTC GGATTCcatt
     2281 gcccagctat ctgtcacttt attgagaaga tagtggaaaa ggaaggtggc tcctacaaat
     2341 gccatcattg cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca
     2401 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt
     2461 caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact
     2521 atccttcgca agacccttcc tctatataag GAAGTTCATT TCATTTGGAG AGGCCTCGAG
     2581 atgAGGATAA ATATCTCTTT GTCTAGTCTC TTTGAGAGAC TGAGCAAATT GTCATCCCGA
     2641 TCCATCGCAA TTACCTGCGG CGTAGTCTTG GCAAGTGCAA TAGCATTCCC AATTATCCGA
     2701 AGAGACTATC AAACGTTCCT TGAAGTCGGT CCGAGCTATG CCCCACAGAA CTTCCGAGGC
     2761 TATATCATCG TTTGTGTCTT GTCACTTTTT AGGCAAgtaa gtttctgctt ctacctttga
     2821 tatatatata ataattatca ttaattagta gtaatataat atttcaaata tttttttcaa
     2881 aataaaagaa tgtagtatat agcaattgct tttctgtagt ttataagtgt gtatatttta
     2941 atttataact tttctaatat atgaccaaaa tttgttgatg tgcagGAGCA GAAAGGGTTG
     3001 GCAATCTATG ACAGGCTTCC AGAAAAGAGG CGTTGGCTTG CCGACTTGCC GTTTCGTGAA
     3061 GGGACGAGGC CATCCATAAC CTCCCACATT ATACAGCGTC AGCGTACTCA GCTGGTAGAT
     3121 CAAGAGTTTG CAACCAGAGA ACTTATCGAT AAGGTGATCC CACGTGTGCA AGCAAGACAC
     3181 ACAGACAAAA CTTTTCTCAG TACGTCAAAA TTTGAGTTTC ATGCAAAGGC CATCTTCCTT
     3241 CTCCCCTCTA TCCCTATTAA TGATCCTCTT AACATTCCCT CACACGATAC GGTAAGAAGA
     3301 ACCAAGCGTG AGATCGCTCA CATGCATGAT TACCATGATT GCACTCTGCA CTTGGCTCTT
     3361 GCTGCTCAGG ATGGTAAGGA AGTTTTGAAG AAGGGGTGGG GCCAGCGTCA CCCACTGGCC
     3421 GGACCAGGAG TCCCAGGCCC TCCTACTGAG TGGACCTTCC TTTACGCACC AAGGAACGAA
     3481 GAGGAGGCCA GAGTTGTCGA GATGATAGTC GAAGCTAGTA TTGGCTACAT GACTAACGAT
     3541 CCTGCTGGTA AGATTGTCGA AAATGCCAAG TCaggttgaG AATTCGGATC CCTGCAGGAT
     3601 CGTTCAAACA TTTGGCAATA aagtttctta agattgaatc ctgttgccgg tcttgcgatg
     3661 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
     3721 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
     3781 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcGGT GTCATCTATG
     3841 TTACTAGATC GGGGagctct ggcaggatat atgcggttgt aattcatttt tattgtctaa
     3901 atttctgtat ttgtttgttt gcaattagat ccacgttgtg tctcaaaatc tctgatgtta
     3961 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     4021 taatacaagg ggtgttatga gccatattca acgggaaacg tcttgttcga ggccgcgatt
     4081 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
     4141 atcaggtgcg acaatctacc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa
     4201 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct
     4261 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg
     4321 gttactcacc actgcgatcc cagggaaaac agcattccag gtattagaag aatatcctga
     4381 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc
     4441 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg
     4501 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt
     4561 tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac
     4621 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat
     4681 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg
     4741 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa
     4801 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt
     4861 ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg gcggctttgt
     4921 tgaatcaatt gatcttcttg aaatcctttt tttctgcgcg taatctgctg cttgcaaaca
     4981 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5041 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     5101 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     5161 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     5221 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     5281 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     5341 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     5401 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     5461 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     5521 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     5581 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     5641 tgatctgatc ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa
     5701 aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc
     5761 aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga
     5821 ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg
     5881 attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa
     5941 ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg
     6001 acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg
     6061 cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacata
//