LOCUS       pLSU-1//t35S[TMV]:nnLuz 6010 bp ds-DNA     circular     24-APR-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     gene            621..1796
                     /label="repA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     rep_origin      1862..2056
                     /label="VS1 ORI"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          2087..2104
                     /label="pLSU-1 5' F"
                     /note="sequence: gcaaaaagcgcctaccct"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2223..2246
                     /label="T-DNA LB Repeat"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          2249..2276
                     /label="315bp t35S F"
                     /note="sequence: ATATTGTCGCATGCGGAAACCTCCTCGGATTC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          2251..2276
                     /label="t35S SphI F"
                     /note="sequence: ATATTGTCGCATGCGGAAACCTCCTCGGATTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    2259..2574
                     /label="315bp t35S"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          2420..2439
                     /label="LBS41 (35S_SF1)"
                     /note="sequence: GAGGAGCATCGTGGAAAAAG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          2458..2485
                     /label="35sF"
                     /note="sequence: CTTCAAAGCAAGTGGATTGATGTGATAT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2551..2579)
                     /label="315bp t35S R"
                     /note="sequence: GACAATATCTCGAGCCTCTCCAAATGAAATGAACTTC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          2551..2598
                     /label="t35s-35s5'UTR-fusion-F"
                     /note="sequence: GAAGTTCATTTCATTTGGAGAGGCACACGCTCGAGTATAAGAGCTCAT"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(2551..2598)
                     /label="t35s-35s5'UTR-fusion-R"
                     /note="sequence: ATGAGCTCTTATACTCGAGCGTGTGCCTCTCCAAATGAAATGAACTTC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2575..2667
                     /label="TMV 5' UTR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="TMV Leader Sequence https://doi.org/10.1016/j.bbrc.2010.11.102 "
     primer          complement(2637..2666)
                     /label="LBS17 (35S 5' Rev Bracket)"
                     /note="sequence: GTATCGATAATTGTAAATGTAATTGTAATG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          2679..2703
                     /label="mLuz-I-f_KpnI"
                     /note="sequence: AAAAGGTACCatgAGGATAAATATCTCTTTGTCTA"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    2679..3488
                     /label="Luz"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             2679..3488
                     /label="Translation 2679-3488"
                     /translation="MRINISLSSLFERLSKLSSRSIAITCGVVLASAIAFPIIRRDYQTFLEVGPSYAPQNFRGYIIVCVLSLFRQEQKGLAIYDRLPEKRRWLADLPFREGTRPSITSHIIQRQRTQLVDQEFATRELIDKVIPRVQARHTDKTFLSTSKFEFHAKAIFLLPSIPINDPLNIPSHDTVRRTKREIAHMHDYHDCTLHLALAAQDGKEVLKKGWGQRHPLAGPGVPGPPTEWTFLYAPRNEEEARVVEMIVEASIGYMTNDPAGKIVENAKSG*"
     misc_feature    2850..2891
                     /label="Buried Alpha-Helix (PredictProtein)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2855..2874
                     /label="A.t. Branch Site (Putative)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(2855..2875)
                     /label="mLUZ-r_br"
                     /note="sequence: ACACAAACGATGATATAGCCT"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2881..2900
                     /label="A.t. Acceptor Splice Site (Putative)"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    2883..2948
                     /label="Conserved AAs (w/ Armillaria)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2892..2954
                     /label="Solvent-Exposed Region (PredictProtein)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    3099..3118
                     /label="A. thaliana Donor Splice Site (Putative)"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          3292..3312
                     /label="mLUZ-f_br"
                     /note="sequence: TTTTGAAGAAGGGGTGGGGCC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(3465..3485)
                     /label="Luz_NarIXhoI_Rev"
                     /note="sequence: AAAACTCGAGACCTGACTTGGCATTTTCGAC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(3466..3485)
                     /label="Fus Luz:T35s Rev V3"
                     /note="sequence: GTGATTTCAGCGAATTCGAGacctGACTTGGCATTTTCGA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(3466..3487)
                     /label="Fus Luz:Int1 Rev V2"
                     /note="sequence: TTCAGTACCAAAAGACAGGCAacctGACTTGGCATTTTCGA"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(3466..3487)
                     /label="CRL4.1"
                     /note="sequence: TTCAGTACCAAAAGACAGGCAACCTGACTTGGCATTTTCGA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(3468..3488)
                     /label="CRL4"
                     /note="sequence: TTCAGTACCAAAAGACAGGCATCAACCTGACTTGGCATTTTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(3468..3488)
                     /label="CRL10.1"
                     /note="sequence: GTGATTTCAGCGAATTCGAGTCAACCTGACTTGGCATTTTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(3468..3488)
                     /label="mLuz-r (MluI)"
                     /note="sequence: TGCATGACGCGTTCAACCTGACTTGGCATTTTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(3468..3488)
                     /label="Fusion Luz-IntF2A-1 Rev"
                     /note="sequence: TTCAGTACCAAAAGACAGGCAtcaacctGACTTGGCATTTTC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(3468..3488)
                     /label="Fus Luz:T35s Rev"
                     /note="sequence: GTGATTTCAGCGAATTCGAGtcaacctGACTTGGCATTTTC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(3468..3488)
                     /label="nnL8 (Fusion nnLuz-pJET Rev)"
                     /note="sequence: ATTATTGTAGGAGATCTTCTAGAAAGATTCAACCTGACTTGGCATTTTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(3468..3488)
                     /label="nnL12 (Luz Rev EcoRI)"
                     /note="sequence: AAAAAGAATTCtcaacctGACTTGGCATTTTC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(3468..3488)
                     /label="mLuz-r"
                     /note="sequence: tgcatgACGCGTtcaacctGACTTGGCATTTTC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(3468..3488)
                     /label="nnL2 (Luz Rev XhoI)"
                     /note="sequence: AAAACTCGAGtcaacctGACTTGGCATTTTC"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(3468..3488)
                     /label="mLUZ-r_XmaI"
                     /note="sequence: AAAACCCGGGtcaacctGACTTGGCATTTTC"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(3468..3489)
                     /label="nnL12 (Luz Rev EcoRI)"
                     /note="sequence: AAAAAGAATTCtcaacctGACTTGGCATTTTC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          3494..3523
                     /label="NosT F"
                     /note="sequence: ATATTGTCCTGCAGGATCGTTCAAACATTTGGCAATA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     regulatory      3501..3756
                     /label="NOS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    3502..3753
                     /label="Nos Terminator"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(3677..3697)
                     /label="NosT-r_br"
                     /note="sequence: attttgttttctatcgcgtat"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(3731..3762)
                     /label="NosT R"
                     /note="sequence: GACAATATGAGCTCCCCGATCTAGTAACATAGATGACACC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3763..3824
                     /label="T-DNA RB Repeat + Overdrive"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    3830..3939
                     /label="Tn903 nptI Promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(3905..3930)
                     /label="pLSU-1 3' R"
                     /note="sequence: tgtattactgtttatgtaagcagaca"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             3940..4755
                     /label="Tn903 nptI CDS"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    4756..4841
                     /label="Tn903 nptI Terminator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     rep_origin      4873..5429
                     /label="ColE1 ORI"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_binding    5502..5524
                     /label="misc_binding"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             5675..294
                     /label="staA CDS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
ORIGIN
        1 tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg
       61 ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt
      121 gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg
      181 agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac
      241 gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat
      301 gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac
      361 gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca
      421 actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca
      481 agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat
      541 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa
      601 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta
      661 agcggctggg ttgtctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg
      721 gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc
      781 tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac
      841 gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc
      901 cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt
      961 tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg
     1021 ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag
     1081 acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc
     1141 tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg
     1201 gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc
     1261 gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca
     1321 cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg
     1381 agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt
     1441 acatcgagat cgagctagtt gattggatgt accgcgagat cacagaaggc aagaacccgg
     1501 acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct
     1561 accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct
     1621 acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga
     1681 tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga
     1741 tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta
     1801 cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggtctctttc
     1861 ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca
     1921 ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag
     1981 agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc
     2041 gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta
     2101 cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg
     2161 gccgctcaaa aatggctggc ctacggccag gcaatctgct agcgcgcgga caagctagga
     2221 ttggcaggat atattcagtt gtaaatGATA TCGCATGCGG AAACCTCCTC GGATTCcatt
     2281 gcccagctat ctgtcacttt attgagaaga tagtggaaaa ggaaggtggc tcctacaaat
     2341 gccatcattg cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca
     2401 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt
     2461 caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact
     2521 atccttcgca agacccttcc tctatataag GAAGTTCATT TCATTTGGAG AGGCACACGC
     2581 TCGAGTATAA GAGCTCATTT TTACAACAAT TACCAACAAC AACAAACAAC AAACAACATT
     2641 ACAATTACAT TTACAATTAT CGATACATCG AGGGTACCat gAGGATAAAT ATCTCTTTGT
     2701 CTAGTCTCTT TGAGAGACTG AGCAAATTGT CATCCCGATC CATCGCAATT ACCTGCGGCG
     2761 TAGTCTTGGC AAGTGCAATA GCATTCCCAA TTATCCGAAG AGACTATCAA ACGTTCCTTG
     2821 AAGTCGGTCC GAGCTATGCC CCACAGAACT TCCGAGGCTA TATCATCGTT TGTGTCTTGT
     2881 CACTTTTTAG GCAAGAGCAG AAAGGGTTGG CAATCTATGA CAGGCTTCCA GAAAAGAGGC
     2941 GTTGGCTTGC CGACTTGCCG TTTCGTGAAG GGACGAGGCC ATCCATAACC TCCCACATTA
     3001 TACAGCGTCA GCGTACTCAG CTGGTAGATC AAGAGTTTGC AACCAGAGAA CTTATCGATA
     3061 AGGTGATCCC ACGTGTGCAA GCAAGACACA CAGACAAAAC TTTTCTCAGT ACGTCAAAAT
     3121 TTGAGTTTCA TGCAAAGGCC ATCTTCCTTC TCCCCTCTAT CCCTATTAAT GATCCTCTTA
     3181 ACATTCCCTC ACACGATACG GTAAGAAGAA CCAAGCGTGA GATCGCTCAC ATGCATGATT
     3241 ACCATGATTG CACTCTGCAC TTGGCTCTTG CTGCTCAGGA TGGTAAGGAA GTTTTGAAGA
     3301 AGGGGTGGGG CCAGCGTCAC CCACTGGCCG GACCAGGAGT CCCAGGCCCT CCTACTGAGT
     3361 GGACCTTCCT TTACGCACCA AGGAACGAAG AGGAGGCCAG AGTTGTCGAG ATGATAGTCG
     3421 AAGCTAGTAT TGGCTACATG ACTAACGATC CTGCTGGTAA GATTGTCGAA AATGCCAAGT
     3481 CaggttgaGG ATCCCTGCAG GATCGTTCAA ACATTTGGCA ATAaagtttc ttaagattga
     3541 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
     3601 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
     3661 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
     3721 tatcgcgcgc GGTGTCATCT ATGTTACTAG ATCGGGGagc tctggcagga tatatgcggt
     3781 tgtaattcat ttttattgtc taaatttctg tatttgtttg tttgcaatta gatccacgtt
     3841 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
     3901 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
     3961 acgtcttgtt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     4021 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct accgattgta tgggaagccc
     4081 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     4141 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     4201 atccgtactc ctgatgatgc atggttactc accactgcga tcccagggaa aacagcattc
     4261 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     4321 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     4381 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     4441 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     4501 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     4561 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     4621 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     4681 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     4741 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg
     4801 acttgacggg acggcggctt tgttgaatca attgatcttc ttgaaatcct ttttttctgc
     4861 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     4921 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     4981 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     5041 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     5101 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     5161 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     5221 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     5281 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     5341 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     5401 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     5461 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     5521 ataaccgtat taccgccttt gagtgatctg atcctgaaga aaccgagcgc cgccgtctaa
     5581 aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc
     5641 gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca
     5701 gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc
     5761 cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc
     5821 cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga
     5881 cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga
     5941 cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc
     6001 ttacgacata
//