LOCUS       pLSU-1//o35S[TMV]:nnLuz 6524 bp ds-DNA     circular     08-NOV-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      1..195
                     /label="VS1 ORI"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          226..243
                     /label="pLSU-1 5' F"
                     /note="sequence: gcaaaaagcgcctaccct"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          362..380
                     /label="TI LB Fwd"
                     /note="sequence: GGCAGGATATATTCAGTTG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    362..385
                     /label="T-DNA LB Repeat"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          388..419
                     /label="35S F"
                     /note="sequence: ATATTGTCGCATGCAGATTAGCCTTTTCAATTTCAG"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          397..420
                     /label="35sP Forward-BamHI"
                     /note="sequence: TTTCGGATCCAGATTAGCCTTTTCAATTTCAGA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          398..417
                     /label="CaMV-35S-1-20-H"
                     /note="sequence: AGATTAGCCTTTTCAATTTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          398..419
                     /label="ToMCP- F"
                     /note="sequence: AGATTAGCCTTTTCAATTTCAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     regulatory      398..1227
                     /label="CaMV o35S"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     1023..1042
                     /label="Binding Region"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          1074..1093
                     /label="LBS41 (35S_SF1)"
                     /note="sequence: GAGGAGCATCGTGGAAAAAG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(1214..1238)
                     /label="35SR"
                     /note="sequence: GACAATATCTCGAGCGTGTTCTCTCCAAATGAA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1228..1320
                     /label="TMV 5' UTR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="TMV Leader Sequence https://doi.org/10.1016/j.bbrc.2010.11.102 "
     primer          complement(1290..1319)
                     /label="LBS17 (35S 5' Rev Bracket)"
                     /note="sequence: GTATCGATAATTGTAAATGTAATTGTAATG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          1332..1356
                     /label="mLuz-I-f_KpnI"
                     /note="sequence: AAAAGGTACCatgAGGATAAATATCTCTTTGTCTA"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1332..2141
                     /label="Luz"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             1332..2141
                     /label="Translation 1332-2141"
                     /translation="MRINISLSSLFERLSKLSSRSIAITCGVVLASAIAFPIIRRDYQTFLEVGPSYAPQNFRGYIIVCVLSLFRQEQKGLAIYDRLPEKRRWLADLPFREGTRPSITSHIIQRQRTQLVDQEFATRELIDKVIPRVQARHTDKTFLSTSKFEFHAKAIFLLPSIPINDPLNIPSHDTVRRTKREIAHMHDYHDCTLHLALAAQDGKEVLKKGWGQRHPLAGPGVPGPPTEWTFLYAPRNEEEARVVEMIVEASIGYMTNDPAGKIVENAKSG*"
     misc_feature    1503..1544
                     /label="Buried Alpha-Helix (PredictProtein)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1508..1527
                     /label="A.t. Branch Site (Putative)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(1508..1528)
                     /label="mLUZ-r_br"
                     /note="sequence: ACACAAACGATGATATAGCCT"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1534..1553
                     /label="A.t. Acceptor Splice Site (Putative)"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    1536..1601
                     /label="Conserved AAs (w/ Armillaria)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1545..1607
                     /label="Solvent-Exposed Region (PredictProtein)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    1752..1771
                     /label="A. thaliana Donor Splice Site (Putative)"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(2121..2141)
                     /label="mLUZ-r_XmaI"
                     /note="sequence: AAAACCCGGGtcaacctGACTTGGCATTTTC"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(2121..2141)
                     /label="nnL12 (Luz Rev EcoRI)"
                     /note="sequence: AAAAAGAATTCtcaacctGACTTGGCATTTTC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(2121..2141)
                     /label="mLuz-r (MluI)"
                     /note="sequence: TGCATGACGCGTTCAACCTGACTTGGCATTTTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2147..2176
                     /label="NosT F"
                     /note="sequence: ATATTGTCCTGCAGGATCGTTCAAACATTTGGCAATA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     regulatory      2154..2409
                     /label="NOS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2155..2406
                     /label="Nos Terminator"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(2384..2415)
                     /label="NosT R"
                     /note="sequence: GACAATATGAGCTCCCCGATCTAGTAACATAGATGACACC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2416..2477
                     /label="T-DNA RB Repeat + Overdrive"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2483..2592
                     /label="Tn903 nptI Promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(2558..2583)
                     /label="pLSU-1 3' R"
                     /note="sequence: tgtattactgtttatgtaagcagaca"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             2593..3408
                     /label="Tn903 nptI CDS"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3409..3494
                     /label="Tn903 nptI Terminator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     rep_origin      3526..4082
                     /label="ColE1 ORI"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_binding    4155..4177
                     /label="misc_binding"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             4328..4957
                     /label="staA CDS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     gene            5284..6459
                     /label="repA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
ORIGIN
        1 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
       61 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
      121 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
      181 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
      241 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
      301 ccgctcaaaa atggctggcc tacggccagg caatctgcta gcgcgcggac aagctaggat
      361 tggcaggata tattcagttg taaatGATAT CGCATGCAGA TTAGCCTTTT CAATTTCAGa
      421 aagaatgcta acccacagat ggttagagag gcttacgcag caggtctcat caagacgatc
      481 tacccgagca ataatctcca ggaaatcaaa taccttccca agaaggttaa agatgcagtc
      541 aaaagattca ggactaactg catcaagaac acagagaaag atatatttct caagatcaga
      601 agtactattc cagtatggac gattcaaggc ttgcttcaca aaccaaggca agtaatagag
      661 attggagtct ctaaaaaggt agttcccact gaatcaaagg ccatggagtc aaagattcaa
      721 atagaggacc taacagaact cgccgtaaag actggcgaac agttcataca gagtctctta
      781 cgactcaatg acaagaagaa aatcttcgtc aacatggtgg agcacgacac acttgtctac
      841 tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa
      901 agggtaatat ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg
      961 aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc
     1021 atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
     1081 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc
     1141 tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata
     1201 taaggaagtt catTTCATTT GGAGAGGACA CGCTCGAGTA TAAGAGCTCA TTTTTACAAC
     1261 AATTACCAAC AACAACAAAC AACAAACAAC ATTACAATTA CATTTACAAT TATCGATACA
     1321 TCGAGGGTAC CatgAGGATA AATATCTCTT TGTCTAGTCT CTTTGAGAGA CTGAGCAAAT
     1381 TGTCATCCCG ATCCATCGCA ATTACCTGCG GCGTAGTCTT GGCAAGTGCA ATAGCATTCC
     1441 CAATTATCCG AAGAGACTAT CAAACGTTCC TTGAAGTCGG TCCGAGCTAT GCCCCACAGA
     1501 ACTTCCGAGG CTATATCATC GTTTGTGTCT TGTCACTTTT TAGGCAAGAG CAGAAAGGGT
     1561 TGGCAATCTA TGACAGGCTT CCAGAAAAGA GGCGTTGGCT TGCCGACTTG CCGTTTCGTG
     1621 AAGGGACGAG GCCATCCATA ACCTCCCACA TTATACAGCG TCAGCGTACT CAGCTGGTAG
     1681 ATCAAGAGTT TGCAACCAGA GAACTTATCG ATAAGGTGAT CCCACGTGTG CAAGCAAGAC
     1741 ACACAGACAA AACTTTTCTC AGTACGTCAA AATTTGAGTT TCATGCAAAG GCCATCTTCC
     1801 TTCTCCCCTC TATCCCTATT AATGATCCTC TTAACATTCC CTCACACGAT ACGGTAAGAA
     1861 GAACCAAGCG TGAGATCGCT CACATGCATG ATTACCATGA TTGCACTCTG CACTTGGCTC
     1921 TTGCTGCTCA GGATGGTAAG GAAGTTTTGA AGAAGGGGTG GGGCCAGCGT CACCCACTGG
     1981 CCGGACCAGG AGTCCCAGGC CCTCCTACTG AGTGGACCTT CCTTTACGCA CCAAGGAACG
     2041 AAGAGGAGGC CAGAGTTGTC GAGATGATAG TCGAAGCTAG TATTGGCTAC ATGACTAACG
     2101 ATCCTGCTGG TAAGATTGTC GAAAATGCCA AGTCaggttg aGGATCCCTG CAGGATCGTT
     2161 CAAACATTTG GCAATAaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta
     2221 tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt
     2281 tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag
     2341 aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcGGTGTCA TCTATGTTAC
     2401 TAGATCGGGG agctctggca ggatatatgc ggttgtaatt catttttatt gtctaaattt
     2461 ctgtatttgt ttgtttgcaa ttagatccac gttgtgtctc aaaatctctg atgttacatt
     2521 gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat
     2581 acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gttcgaggcc gcgattaaat
     2641 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     2701 ggtgcgacaa tctaccgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     2761 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     2821 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     2881 ctcaccactg cgatcccagg gaaaacagca ttccaggtat tagaagaata tcctgattca
     2941 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     3001 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     3061 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     3121 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
     3181 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     3241 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     3301 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     3361 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt
     3421 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgg ctttgttgaa
     3481 tcaattgatc ttcttgaaat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     3541 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     3601 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     3661 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3721 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3781 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3841 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     3901 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3961 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4021 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     4081 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     4141 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgat
     4201 ctgatcctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca
     4261 gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa atacgcaagg
     4321 ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca agacgaccat
     4381 cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt tagtcgattc
     4441 cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt
     4501 tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt
     4561 cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga tcaaggcagc
     4621 cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca ccgccgacct
     4681 ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag cggcctttgt
     4741 cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg cgctggccgg
     4801 gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc caggcactgc
     4861 cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc gcgaggtcca
     4921 ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa agagaaaatg
     4981 agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag caaggctgca
     5041 acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca gttgccggcg
     5101 gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat taccgagctg
     5161 ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa tgagtagatg
     5221 aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca ccgacgccgt
     5281 ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct gggttgtctg
     5341 ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa
     5401 ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag
     5461 gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg
     5521 caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg
     5581 tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat
     5641 gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag
     5701 cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt
     5761 tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt
     5821 tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc
     5881 gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag
     5941 cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag
     6001 cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt
     6061 agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta
     6121 gttgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac
     6181 cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc
     6241 gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc
     6301 gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg
     6361 ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac
     6421 cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg
     6481 caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcc
//