LOCUS       pLSU2//{PepGMV}mLIR:mNG 7215 bp ds-DNA     circular     30-AUG-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      1..195
                     /label="VS1 ORI"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          226..243
                     /label="pLSU-1 5' Fwd"
                     /note="sequence: gcaaaaagcgcctaccct"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    362..385
                     /label="T-DNA LB Repeat"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(372..397)
                     /label="pLSU2-->1_R"
                     /note="sequence: AAAAAAAGCTTGCATGCGATATCatttacaactgaat"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    398..676
                     /label="tmlP"
                     /ApEinfo_revcolor="#a2d39c"
                     /ApEinfo_fwdcolor="#a2d39c"
     misc_feature    687..1481
                     /label="NPTII CDS"
                     /ApEinfo_revcolor="#a2d39c"
                     /ApEinfo_fwdcolor="#a2d39c"
     CDS             687..1491
                     /label="Translation 687-1491"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*AGL"
     primer          699..718
                     /label="NPTII-F"
                     /note="sequence: GATGGATTGCACGCAGGTTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          699..718
                     /label="UK-nptII-F"
                     /note="sequence: gatggattgcacgcaggttc"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          1263..1282
                     /label="dPCRnptII F"
                     /note="sequence: TTGCCGAATATCATGGTGGA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(1277..1296)
                     /label="NPTII-R"
                     /note="sequence: AAAAGCGGCCATTTTCCACC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(1277..1296)
                     /label="UK-nptII-R"
                     /note="sequence: aaaagcggccattttccacc"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(1356..1375)
                     /label="dPCRnptII R"
                     /note="sequence: TCAGCAATATCACGGGTAGC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    1498..1775
                     /label="tmlT"
                     /ApEinfo_revcolor="#a2d39c"
                     /ApEinfo_fwdcolor="#a2d39c"
     primer          1591..1611
                     /label="pLSU2-f_br"
                     /note="sequence: AAATAAACGTTATCCTCTTCT"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          1659..1681
                     /label="pLSU-2 5' Fwd"
                     /note="sequence: TTCTAAAAGTGTTCTAAGCGGGC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          1659..1681
                     /label="pLSU2-4 5' Fwd"
                     /note="sequence: TTCTAAAAGTGTTCTAAGCGGGC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          1764..1782
                     /label="pLSU2-->1_F"
                     /note="sequence: AAGCTTCCATGGCTCGAGG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          1776..1802
                     /label="mLIR:nLUC-f"
                     /note="sequence: GTACGTACGTCTCGAGggaattctctgcctgacgcac"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          1782..1799
                     /label="mLIR cloning FW"
                     /note="sequence: AAAGAGCTCGGAATTCTCTGCCTGACG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          1782..1801
                     /label="mLIR Forward-BamHI"
                     /note="sequence: TTTCGGATCCGGAATTCTCTGCCTGACGCA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    1782..1811
                     /label="RP7"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     gene            complement(1782..1832)
                     /label="AC3 gene"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             complement(1782..1832)
                     /label="AC3 CDS"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     source          1782..1885
                     /label="TrAP PepGMV"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     gene            complement(1782..1885)
                     /label="AC2 gene"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             complement(1782..1885)
                     /label="AC2 CDS"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          1789..1806
                     /label="TNPF"
                     /note="sequence: TCTGCCTGACGCACAGTG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    1797..1826
                     /label="RP3"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1827..1856
                     /label="RP4"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1857..1885
                     /label="RP5"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     source          1892..2096
                     /label="LIR PepGMV"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1897..1917
                     /label="mCP_mLIR_BR_F"
                     /note="sequence: ggccgcgatttttcggagtcc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          1903..1923
                     /label="mLIR-f_br"
                     /note="sequence: gatttttcggagtccttgggg"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(1958..1978)
                     /label="PepGMV_mLIR-r_br"
                     /note="sequence: attggtggtcccctctcttta"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          1998..2019
                     /label="pTPV 5' Fwd"
                     /note="sequence: aatcacatcccgcctgtcaagt"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2075..2096)
                     /label="mLIR Reverse-ScaI"
                     /note="sequence: TTTCAGTACTTTTGAATTAAAGGTCTAAATGC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2127..2149
                     /label="mNG11 (mNG Fwd ClaI)"
                     /note="sequence: AAAAATCGATATGGTTTCCAAAGGTGAGGAAG"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          2128..2145
                     /label="mNeonTomato-XbaI-F"
                     /note="sequence: TTTCCCTCTAGAATGGTTTCCAAAGGTGAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2128..2145
                     /label="(AscI) mNG F"
                     /note="sequence: ATGATACTGGCGCGCCATGGTTTCCAAAGGTGAG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          2128..2145
                     /label="(BbvC.MCS2I) mNG F"
                     /note="sequence: ATGATACTCCTCAGCATGGTTTCCAAAGGTGAG"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          2128..2145
                     /label="(XhoI) mNG F"
                     /note="sequence: ATATAGCTCTCGAGATGGTTTCCAAAGGTGAG"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          2128..2146
                     /label="mNeonGreen BsrGI Fwd"
                     /note="sequence: CACTGTACACAACATGGTTTCCAAAGGTGAGG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          2128..2149
                     /label="mNeon fwd xhoI"
                     /note="sequence: TCGAACCTCGAGATGGTTTCCAAAGGTGAGGAAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2128..2149
                     /label="mNG09 (mNG Fwd XhoI)"
                     /note="sequence: AAAACTCGAGATGGTTTCCAAAGGTGAGGAAG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2128..2838
                     /label="mNeonGreen @Tomato"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             2128..2838
                     /label="Translation 2128-2838"
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDGASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIISTFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQKAFTDVMGMDELYK*"
     primer          complement(2304..2324)
                     /label="mNG-r_br"
                     /note="sequence: TGAGGGACTAGGATCCATGGG"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          2306..2325
                     /label="mNG14 (mNG 5' Seq Fwd)"
                     /note="sequence: CATGGATCCTAGTCCCTCAC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          2306..2325
                     /label="mNG14"
                     /note="sequence: CATGGATCCTAGTCCCTCAC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2306..2325)
                     /label="mNG07 (mNG 5' Rev Bracket)"
                     /note="sequence: GTGAGGGACTAGGATCCATG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          2323..2343
                     /label="mNG_fusion_F"
                     /note="sequence: cacattggatacggcttccac"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(2814..2838)
                     /label="mNG10 (mNG Rev EcoRI)"
                     /note="sequence: AAAAGAATTCTTACTTATATAACTCGTCCATTCCC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(2814..2838)
                     /label="(AvrII.MCS2) mNG R"
                     /note="sequence: GTCCTTCCCCTAGGTTACTTATATAACTCGTCCATTCCC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(2814..2838)
                     /label="mNeonTomato-SacI-R"
                     /note="sequence: TTTTCCCGAGCTCTTACTTATATAACTCGTCCATTCCC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2814..2839)
                     /label="mNG12 (mNG Rev XhoI)"
                     /note="sequence: AAAACTCGAGTTACTTATATAACTCGTCCATTCCC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(2815..2838)
                     /label="(SbfI) mNG R"
                     /note="sequence: ATATTCCCTGCAGGTTACTTATATAACTCGTCCATTCC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(2815..2838)
                     /label="(PstI) mNG R"
                     /note="sequence: GTCCTCCTGCAGTTACTTATATAACTCGTCCATTCC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(2819..2838)
                     /label="mNG Rev BamHI"
                     /note="sequence: cgcgGGATCCTTACTTATATAACTCGTCCA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     regulatory      2845..3100
                     /label="NOS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2845..3100
                     /label="Digestion of Digestion of pBI121 by BamHI by SacI- Fragment 2 (concatenated sequence 1)"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2846..3097
                     /label="Nos Terminator"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          2904..2924
                     /label="NosT-f_br"
                     /note="sequence: gatgattatcatataatttct"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(3080..3106)
                     /label="NosT-r (SacI)"
                     /note="sequence: AAAGAGCTCCCCGATCTAGTAACATAGATG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    3107..3168
                     /label="T-DNA RB Repeat + Overdrive"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    3174..3283
                     /label="Tn903 nptI Promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(3249..3274)
                     /label="pLSU-1 3' Rev"
                     /note="sequence: tgtattactgtttatgtaagcagaca"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(3283..3303)
                     /label="pLSU2-r_br"
                     /note="sequence: TCTTCAAGAGTAAACACCATa"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          3284..3304
                     /label="NPT1"
                     /note="sequence: ATGAGCCATATTCAACGGGAA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     CDS             3284..4099
                     /label="Tn903 nptI CDS"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(3291..3310)
                     /label="BP NptI R"
                     /note="sequence: AGACGTTTCCCGTTGAATAT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    4100..4185
                     /label="Tn903 nptI Terminator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     rep_origin      4217..4773
                     /label="ColE1 ORI"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_binding    4846..4868
                     /label="misc_binding"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             5019..5648
                     /label="staA CDS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     gene            5975..7150
                     /label="repA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
ORIGIN
        1 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
       61 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
      121 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
      181 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
      241 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
      301 ccgctcaaaa atggctggcc tacggccagg caatctgcta gcgcgcggac aagctaggat
      361 tggcaggata tattcagttg taaatGATAT CGCATGCtat gcagcatttt tttggtgtat
      421 gtgggcCCCA AATGAAGTGC AGGTCAAACC TTGACAGTGA CGACAAATCG TTGGGCGGGT
      481 CCAGGGCGAA TTTTGCGACA ACATGTCGAG GCTCAGCAGC TGAAAATTCA AACGCGCTAG
      541 TCAATGCTAT CAATCTGTCG TGTTCACACG GCATCAAACG GTCACCAATG ACGTCAATGG
      601 GCTTCCTTAG TCAATATAAA AACCAACGGC TCAGACTTAC CAGCGGCAGG TATTTgtagt
      661 acatccaaca ctgcccTGAT CTtcgcatga ttgaacaaga tggattgcac gcaggttctc
      721 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
      781 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
      841 acctgtccgg tgccctgaat gaactccagg acgaggcagc gcggctatcg tggctggcca
      901 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
      961 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     1021 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     1081 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     1141 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     1201 ccaggctcaa ggcgcggatg cccgacggcg aggatctcgt cgtgacacat ggcgatgcct
     1261 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     1321 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     1381 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     1441 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc cAGATCAccg
     1501 tcgccgacac ctaataaagt cGGCTAATCT ATGTGATTGA GTGTGTCTTG ACTTTGTTAT
     1561 TTTGCATGTT TCCAATGTCA TTTAGTAACG AAATAAACGT TATCCTCTTC TAAAAGCAGG
     1621 CTGTGTTTTC GGCAAACATC GCCACCCATC GCTAGTTTTT CTAAAAGTGT TCTAAGCGGG
     1681 CCTGGTAATA ATCTATACGA GCTTATATTT CTAATCATTG CCGAAAAATC CTGTTTCGAA
     1741 ATAATTTTGT AActctcttt aatatcacca cgagcAagct tggaattctc tgcctgacgc
     1801 acagtgatgc tctccccggt gcgtgaatcc attatttgcg cagttgatgt ggaggaagat
     1861 agagcagccg cagtttaggt ctattGGATC Ccggatggcc gcgatttttc ggagtccttg
     1921 ggggaccact cagaatagag ggctgagatt aaatagataa agagagggga ccaccaatta
     1981 tttgattttg actgaccaat cacatcccgc ctgtcaagtc tagatatttg tgccttggtg
     2041 accaaggtgt ggtcctataa atgcacgtgc atgtgcattt agacctttaa ttcaaaACGC
     2101 GTGCTCGAGG GTACCACTAG TGAATTCATG GTTTCCAAAG GTGAGGAAGA TAATATGGCT
     2161 AGTTTACCTG CCACACATGA GCTACACATA TTCGGCAGCA TAAACGGTGT AGATTTTGAT
     2221 ATGGTTGGAC AAGGGACAGG TAATCCTAAT GATGGATATG AGGAGTTGAA CCTCAAATCT
     2281 ACCAAGGGGG ACCTTCAATT CTCCCCATGG ATCCTAGTCC CTCACATTGG ATACGGCTTC
     2341 CACCAGTACT TACCATACCC AGATGGAATG TCCCCTTTCC AAGCAGCCAT GGTTGACGGT
     2401 TCCGGGTACC AGGTACATAG GACAATGCAA TTTGAGGACG GGGCTTCTCT GACTGTGAAT
     2461 TACCGATATA CCTATGAAGG CAGTCACATA AAGGGCGAAG CACAAGTGAA GGGCACCGGA
     2521 TTTCCTGCCG ACGGGCCTGT AATGACTAAT AGCTTGACCG CCGCAGATTG GTGCAGGTCA
     2581 AAAAAGACCT ACCCAAATGA CAAGACAATA ATCTCTACAT TTAAGTGGAG CTACACTACA
     2641 GGGAATGGTA AACGATATAG ATCAACTGCT AGAACCACAT ATACCTTCGC TAAACCTATG
     2701 GCAGCCAATT ATCTGAAGAA CCAGCCCATG TATGTGTTCC GAAAGACTGA ACTCAAGCAT
     2761 AGCAAAACCG AACTCAACTT CAAAGAGTGG CAAAAGGCTT TCACTGACGT GATGGGAATG
     2821 GACGAGTTAT ATAAGTAACC CGGGGATCGT TCAAACATTT GGCAATAaag tttcttaaga
     2881 ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag
     2941 catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga
     3001 gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat
     3061 aaattatcgc gcgcGGTGTC ATCTATGTTA CTAGATCGGG GAGCTCtggc aggatatatg
     3121 cggttgtaat tcatttttat tgtctaaatt tctgtatttg tttgtttgca attagatcca
     3181 cgttgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac
     3241 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg
     3301 ggaaacgtct tgttcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
     3361 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctaccgat tgtatgggaa
     3421 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
     3481 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca
     3541 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatcccag ggaaaacagc
     3601 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
     3661 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt
     3721 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt
     3781 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt
     3841 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
     3901 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata
     3961 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg
     4021 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat
     4081 gctcgatgag tttttctaat cagaattggt taattggttg taacactggc agagcattac
     4141 gctgacttga cgggacggcg gctttgttga atcaattgat cttcttgaaa tccttttttt
     4201 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     4261 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     4321 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     4381 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     4441 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     4501 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     4561 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     4621 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     4681 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     4741 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     4801 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     4861 gtggataacc gtattaccgc ctttgagtga tctgatcctg aagaaaccga gcgccgccgt
     4921 ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg
     4981 atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta
     5041 accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac
     5101 tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg
     5161 cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc
     5221 gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg
     5281 cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa
     5341 gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg
     5401 tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca
     5461 tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta
     5521 tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag
     5581 aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac
     5641 tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg
     5701 ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc
     5761 catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc
     5821 ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga
     5881 gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg
     5941 aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg
     6001 gttggccagg cgtaagcggc tgggttgtct gccggccctg caatggcact ggaaccccca
     6061 agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc
     6121 gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat
     6181 cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga
     6241 atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga
     6301 gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat
     6361 catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg
     6421 ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggccagtgt
     6481 gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata
     6541 ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact
     6601 caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat
     6661 tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct
     6721 ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac
     6781 cgggcggccg gagtacatcg agatcgagct agttgattgg atgtaccgcg agatcacaga
     6841 aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat
     6901 cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt
     6961 gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac
     7021 cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg
     7081 gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc
     7141 cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg
     7201 aaaaggtctc tttcc
//