LOCUS       pCUP1-∆ssCPYstar        7776 bp DNA     circular SYN 21-NOV-2023
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pCUP1-∆ssCPY*-mGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 21, 2023 from SnapGene Viewer 7.0.3
            https://www.snapgene.com
COMMENT     7776 bp  DNA
COMMENT     Alias: pCUP1-∆ssCPY*-mGFP
FEATURES             Location/Qualifiers
     source          1..7776
                     /label=source 1
                     /organism="synthetic DNA construct"
     promoter        190..410
                     /label=URA3 promoter
     misc_feature    411..1214
                     /label=URA3 ORF
                     /note="note=""URA3 ORF"" - 411..1214"
     promoter        2011..2421
                     /label=CUP1 promoter
                     /label=CUP1
                     /note="note=""CUP1 promoter"" - 2011..2421"
     primer_bind     2327..2356
                     /label=Primer 1
                     /note="Foward sequencing primer"
     misc_feature    2423..3958
                     /label=∆ssCPY*
                     /note="dnas_title=""∆ssCPY*"" - note="" - 2423..3958"
     misc_feature    3959..3964
                     /label=Linker 2
                     /label=A LINKER
                     /note="note=""A LINKER"" - 3959..3964"
     misc_feature    3965..4678
                     /label=mGFP
                     /note="note=""eGFP F64L / G65T / A72S / Q80R / A206V"" - 
                     3965..4678"
     misc_feature    4577..4579
                     /label=Mutation mGFP
                     /label=MUTA mGFP
     misc_feature    4685..4699
                     /label=Linker 1
                     /label=CHRIS LINKER
                     /note="note=""CHRIS LINKER"" - 4688..4702"
     misc_feature    4706..4980
                     /label=CYC terminator
                     /note="note=""CYC terminator"" - 4709..4983"
     misc_feature    4784..4813
                     /label=Reverse sequencing primer
     primer_bind     complement(4784..4813)
                     /label=Primer 2
                     /note="Reverse sequencing primer"
     promoter        5002..5020
                     /label=T7 promoter
     misc_feature    5443..6031
                     /label=ori
     misc_feature    6202..7062
                     /label=AmpR
     promoter        7063..7167
                     /label=Amp promoter
     misc_feature    7204..7707
                     /label=CEN/ARS
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc
      241 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca
      301 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat
      361 tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc atgtcgaaag
      421 ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata
      481 tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat
      541 tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata
      601 tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt
      661 acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc
      721 agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg
      781 tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac
      841 ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat
      901 atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg
      961 ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg
     1021 tgggtttaga tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg
     1081 tctctacagg atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg
     1141 ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg
     1201 gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta
     1261 gagcttcaat ttaattatat cagttattac cctgcggtgt gaaataccgc acagatgcgt
     1321 aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa attcgcgtta
     1381 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     1441 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     1501 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     1561 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     1621 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     1681 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     1741 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg
     1801 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     1861 ctattacgcc agctggcgaa ggggggatgt gctgcaaggc gattaagttg ggtaacgcca
     1921 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta
     1981 tagggcgaat tggagctcca ccgcggtggc ggccgcatgt tcatgtatgt atctgtattt
     2041 aaaacacttt tgtattattt ttcctcatat atgtgtatag gtttatacgg atgatttaat
     2101 tattacttca ccacccttta tttcaggctg atatcttagc cttgttacta gttagaaaaa
     2161 gacatttttg ctgtcagtca ctgtcaagag attcttttgc tggcatttct tctagaagca
     2221 aaaagagcga tgcgtctttt ccgctgaacc gttccagcaa aaaagactac caacgcaata
     2281 tggattgtca gaatcatata aaagagaagc aaataactcc ttgtcttgta tcaattgcat
     2341 tataatatct tcttgttagt gcaatatcat atagaagtca tcgaaataga tattaagaaa
     2401 aacaaactgt aacgaattca tgatctcatt gcaaagaccg ttgggtctag ataaggacgt
     2461 tttgctgcaa gctgcggaaa aatttggttt ggacctcgac ctggatcatc tcttgaagga
     2521 gttggactcc aatgtattgg acgcttgggc ccaaatagag catttgtacc caaaccaggt
     2581 tatgagcctt gaaacttcca ctaagccaaa attccctgaa gcaatcaaaa cgaagaaaga
     2641 ctgggacttt gtggtcaaga atgacgcaat tgaaaactat cagcttcgtg tcaacaagat
     2701 taaggaccct aaaatcctgg gcattgaccc aaatgtcaca cagtacacgg gttacttgga
     2761 tgtggaagac gaggacaagc atttcttctt ttggactttt gaaagtagaa acgatcctgc
     2821 aaaggatccg gtcatccttt ggttgaacgg gggtccaggt tgttcttcac taaccgggct
     2881 gttctttgaa ttaggaccct catccattgg acctgatttg aaacccatcg ggaaccctta
     2941 ctcttggaac agcaatgcca ccgtgatctt ccttgaccag cctgtcaacg ttgggttctc
     3001 gtattccggg tcctcaggtg tttccaacac tgtcgccgct ggtaaggatg tctataactt
     3061 cttggagttg ttcttcgatc agttccctga atacgtcaac aagggccaag atttccacat
     3121 cgctagggaa tcctacgccg gccattacat ccctgttttt gcctctgaaa ttttgtctca
     3181 caaggacaga aacttcaact taacctccgt cttgatcgga aatggcctca ctgacccatt
     3241 gactcagtat aactattacg aaccaatggc ctgtggtgaa ggtggcgaac catctgtttt
     3301 gccctcggag gaatgctctg ctatggaaga ctctttggaa cgttgtttgg gcttgatcga
     3361 gtcgtgctat gactcgcaat ccgtctggtc ctgtgttcca gctaccattt attgtaataa
     3421 cgcccaattg gctccttacc aacgtaccgg cagaaacgtt tacgatatca ggaaggattg
     3481 tgaaggtggc aatttgtgct acccaacgtt acaagatatc gacgactact taaaccagga
     3541 ctacgtcaaa gaagctgtcg gtgcggaggt tgaccactac gaatcctgta acttcgatat
     3601 caacagaaat ttcctgtttg cgggtgattg gatgaagcct taccacaccg ccgtaacaga
     3661 tcttttgaat caagacctac ccattctggt atatgcaggc gataaagatt tcatctgtaa
     3721 ctggttgggt aataaggcgt ggacggatgt cttgccatgg aagtacgacg aagaatttgc
     3781 aagccaaaaa gtacgtaact ggactgcttc tatcaccgac gaggtcgctg gtgaagtcaa
     3841 atcctacaag cacttcacct atttgagagt cttcaatggt ggccacatgg ttccatttga
     3901 cgtccctgaa aacgccttaa gtatggttaa cgaatggatc cacggtggtt tctccttact
     3961 cgagagtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
     4021 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
     4081 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
     4141 tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca
     4201 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
     4261 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
     4321 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
     4381 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
     4441 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
     4501 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
     4561 cctgtccaca caatctaaac tttcgaaaga tcccaacgaa aagagagacc acatggtcct
     4621 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaataaaa
     4681 gctttgccat agaatatctg gatccggtac cgggttgaag aaggaaaaag acagaaacga
     4741 cttaattacc tacttgaaaa aagcctgtga gtaaacaggc cccttttcct ttgtcgatat
     4801 catgtaatta gttatgtcac gcttacattc acgccctcct cccacatccg ctctaaccga
     4861 aaaggaagga gttagacaac ctgaagtcta ggtccctatt tatttttttt aatagttatg
     4921 ttagtattaa gaacgttatt tatatttcaa atttttcttt tttttctgta caaacgcgtg
     4981 tacggtaccc agcttttgtt ccctttagtg agggttaatt ccgagcttgg cgtaatcatg
     5041 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acataggagc
     5101 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg aggtaactca cattaattgc
     5161 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     5221 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     5281 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     5341 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     5401 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctcggccc
     5461 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     5521 ataaagatac caggcgttcc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     5581 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg
     5641 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     5701 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     5761 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     5821 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     5881 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     5941 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     6001 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     6061 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     6121 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     6181 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     6241 ctgtctattt cgttcatcca tagttgcctg actgcccgtc gtgtagataa ctacgatacg
     6301 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     6361 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     6421 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     6481 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     6541 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     6601 ccccatgttg tgaaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     6661 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     6721 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     6781 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     6841 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     6901 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     6961 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     7021 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     7081 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     7141 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgggtcctt
     7201 ttcatcacgt gctataaaaa taattataat ttaaattttt taatataaat atataaatta
     7261 aaaatagaaa gtaaaaaaag aaattaaaga aaaaatagtt tttgttttcc gaagatgtaa
     7321 aagactctag ggggatcgcc aacaaatact accttttatc ttgctcttcc tgctctcagg
     7381 tattaatgcc gaattgtttc atcttgtctg tgtagaagac cacacacgaa aatcctgtga
     7441 ttttacattt tacttatcgt taatcgaatg tatatctatt taatctgctt ttcttgtcta
     7501 ataaatatat atgtaaagta cgctttttgt tgaaattttt taaacctttg tttatttttt
     7561 tttcttcatt ccgtaactct tctaccttct ttatttactt tctaaaatcc aaatacaaaa
     7621 cataaaaata aataaacaca gagtaaattc ccaaattatt ccatcattaa aagatacgag
     7681 gcgcgtgtaa gttacaggca agcgatccgt cctaagaaac cattattatc atgacattaa
     7741 cctataaaaa taggcgtatc acgaggccct ttcgtc
//
