LOCUS       CROPseq-Puro-2xMS2     10373 bp ds-DNA     circular     15-NOV-2023
DEFINITION  .
KEYWORDS    "accession:addgene_86708_209125"
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
FEATURES             Location/Qualifiers
     promoter        288..514
                     /label="RSV promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     LTR             515..695
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    742..867
                     /label="HIV-1 Psi"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1360..1593
                     /label="RRE"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2003..2120
                     /label="cPPT/CTS"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        2179..3437
                     /label="EF-1a promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2278..2489
                     /label="EF-1a core promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     intron          2490..3428
                     /label="EF-1a intron A"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    3444..3452
                     /label="kozak"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             3450..4049
                     /label="PuroR"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             3450..4049
                     /label="Translation 3450-4049"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     misc_feature    4106..4124
                     /label="MS2 Hairpin"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    4146..4164
                     /label="MS2 Hairpin"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    4214..4802
                     /label="WPRE"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    4873..4908
                     /label="3' LTR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    4958..5206
                     /label="hU6 promoter"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    5207..5207
                     /label="TSS"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    5207..7087
                     /label="stuffer"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    7088..7173
                     /label="scaffold_Dang_2015"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    7092..7092
                     /label="Dang_2015_M"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    7100..7104
                     /label="Dang_2015_plus-5"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    7105..7108
                     /label="loop 1"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    7109..7113
                     /label="Dang_2015_plus-5"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    7123..7123
                     /label="Dang_2015_M"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    7174..7179
                     /label="pol III termination"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     LTR             7226..7406
                     /label="3' LTR (truncated)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal    7484..7605
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     rep_origin      7645..7780
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     rep_origin      7987..8442
                     /label="f1 ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     promoter        8468..8572
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             8573..9433
                     /label="AmpR"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             8573..9433
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     rep_origin      9604..10192
                     /label="ori"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
      241 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
      301 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
      361 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
      421 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
      481 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
      541 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      601 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      661 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      721 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      781 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      841 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      901 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      961 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     1021 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1081 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1141 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1201 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1261 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1321 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1381 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1441 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1501 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1561 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1621 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1681 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1741 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1801 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1861 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1921 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga atcttgagac
     1981 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2041 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2101 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc
     2161 ggctcgaggg ggcccgggtg caaagatgga taaagtttta aacagagagg aatctttgca
     2221 gctaatggac cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg
     2281 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgatccg
     2341 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2401 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2461 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2521 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2581 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2641 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     2701 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     2761 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     2821 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     2881 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     2941 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg
     3001 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     3061 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     3121 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     3181 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     3241 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3301 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3361 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3421 catttcaggt gtcgtgacgt acggccacca tgaccgagta caagcccacg gtgcgcctcg
     3481 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     3541 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     3601 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     3661 ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     3721 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     3781 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggagtct
     3841 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     3901 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     3961 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     4021 ggtgcatgac ccgcaagccc ggtgcctgaT GGACGAGCTG TACAAGTAAA GCGGCCGGAT
     4081 CCTAAGGTAC CTAATTGCCT AGAAAACATG AGGATCACCC ATGTCTGCAG GTCGGACTCT
     4141 AGAAAACATG AGGATCACCC ATGTCTGCAG TATTCCCGGG TTCATTAGAT CTGCCGCGCG
     4201 ATCGATAGAT CCTaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     4261 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     4321 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     4381 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     4441 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     4501 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     4561 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     4621 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     4681 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     4741 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     4801 gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     4861 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagattt caccattatc
     4921 gtttcagacc cacctcccaa ccccgagggg acccagagag ggcctatttc ccatgattcc
     4981 ttcatatttg catatacgat acaaggctgt tagagagata attagaatta atttgactgt
     5041 aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt
     5101 tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta
     5161 tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccggag acggttgtaa
     5221 atgagcacac aaaatacaca tgctaaaata ttatattcta tgacctttat aaaatcaacc
     5281 aaaatcttct ttttaataac tttagtatca ataattagaa tttttatgtt cctttttgca
     5341 aacttttaat aaaaatgagc aaaataaaaa aacgctagtt ttagtaactc gcgttgtttt
     5401 cttcaccttt aataatagct actccaccac ttgttcctaa gcggtcagct cctgcttcaa
     5461 tcattttttg agcatcttca aatgttctaa ctccaccagc tgctttaact aaagcattgt
     5521 ctttaacaac tgacttcatt agtttaacat cttcaaatgt tgcacctgat tttgaaaatc
     5581 ctgttgatgt tttaacaaat tctaatccag cttcaacagc tatttcacaa gctttcatga
     5641 tttcttcttt tgttaataaa caattttcca taatacattt aacaacatgt gatccagctg
     5701 ctttttttac agctttcatg tcttctaaaa ctaattcata atttttgtct tttaatgcac
     5761 caatatttaa taccatatca atttctgttg caccatcttt aattgcttca gaaacttcga
     5821 atgcttttgt agctgttgtg catgcaccta gaggaaaacc tacaacattt gttattccta
     5881 catttgtgcc ttttaataat tctttacaat agcttgttca atatgaatta acacaaactg
     5941 ttgcaaaatc aaattcaatt gcttcatcac ataattgttt aatttcagct ttcgtagcat
     6001 cttgttttaa taatgtgtga tctatatatt tgtttagttt cattttttct cctatatatt
     6061 catttttaat tttaattctt taataatttc gtctacttta actttagcgt tttgaacaga
     6121 ttcaccaaca cctataaaat aaatttttag tttaggttca gttccacttg ggcgaacagc
     6181 aaatcatgac ttatcttcta aataaaattt tagtaagtct tgtcctggca tattatacat
     6241 tccatcgatg tagtcttcaa cattaacaac tttaagtcca gcaatttgag ttaagggtgt
     6301 tgctctcaat gatttcatta atggttcaat ttttaatttc ttttcttctg gtttaaaatt
     6361 caagtttaaa gtgaaagtgt aatatgcacc catttcttta aataaatctt ctaaatagtc
     6421 tactaatgtt ttattttgtt ttttataaaa tcaagcagcc tctgctatta atatagaagc
     6481 ttgtattcca tctttatctc tagctgagtc atcaattaca tatccataac tttcttcata
     6541 agcaaaaaca aaatttaatc cgttatcttc ttctttagca atttctctac ccattcattt
     6601 aaatccagtt aaagttttta caatattaac tccatatttt tcatgagcga ttctatcacc
     6661 caaatcactt gttacaaaac ttgaatatag agccggattt tttggaatgc tatttaagcg
     6721 ttttagattt gataattttc aatcaattaa aattggtcct gtttgatttc catctaatct
     6781 tacaaaatga ccatcatgtt ttattgccat tccaaatctg tcagcatctg ggtcattcat
     6841 aataataata tctgcatcat gtttaatacc atattcaagc ggtatttttc atgcaggatc
     6901 aaattctgga tttggattta caacattttt aaatgtttca tcttcaaatg catgctcttc
     6961 aacctcaata acgttatatc ctgattcacg taatattttt ggggtaaatt tagttcctgt
     7021 tccattaact gcgctaaaaa taatttttaa atctttttta gcttcttgct cttttttgta
     7081 cgtctctGTT TCAGAGCTAT GCTGGAAACA GCATAGCAAG TTGAAATAAG GCTAGTCCGT
     7141 TATCAACTTG AAAAAGTGGC ACCGAGTCGG Tgctttttta agcttggcgt aactagatct
     7201 tgagacactg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     7261 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     7321 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     7381 ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca
     7441 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     7501 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7561 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     7621 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     7681 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     7741 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     7801 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     7861 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     7921 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     7981 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     8041 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     8101 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     8161 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     8221 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     8281 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     8341 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     8401 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     8461 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     8521 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     8581 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     8641 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     8701 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     8761 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     8821 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     8881 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     8941 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     9001 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     9061 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     9121 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     9181 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     9241 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     9301 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     9361 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     9421 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     9481 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     9541 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     9601 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     9661 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     9721 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     9781 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     9841 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     9901 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     9961 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
    10021 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
    10081 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    10141 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
    10201 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
    10261 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
    10321 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aag
//