LOCUS       pGag-mCherry_(pxN01-mCh 7031 bp ds-DNA     circular     15-NOV-2023
DEFINITION  .
KEYWORDS    "Test:Functional"
FEATURES             Location/Qualifiers
     promoter        83..658
                     /label="CMV_immearly_promoter"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    83..1319
                     /label="CAG promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    161..448
                     /label="CAG_enhancer"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        616..685
                     /label="CMV_promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    936..1319
                     /label="bGlob_int"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    1329..2918
                     /label="MLV Gag (UniProt ID: P03332)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             1329..3647
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MGQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTFNVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKPPPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGAKPKPQVLSDSGGPLIDLLTEDPPPYRDPRPPPSDRDGNGGEATPAGEAPDPSPMASRLRGRREPPVADSTTSQAFPLRAGGNGQLQYWPFSSSDLYNWKNNNPSFSEDPGKLTALIESVLITHQPTWDDCQQLLGTLLTGEEKQRVLLEARKAVRGDDGRPTQLPNEVDAAFPLERPDWDYTTQAGRNHLVHYRQLLLAGLQNAGRSPTNLAKVKGITQGPNESPSAFLERLKEAYRRYTPYDPEDPGQETNVSMSFIWQSAPDIGRKLERLEDLKNKTLGDLVREAEKIFNKRETPEEREERIRRETEEKEERRRTEDEQKEKERDRRRHREMSKLLATVVSGQKQDRQGGERRRSQLDRDQCAYCKEKGHWAKDCPKKPRGPRGPRPQGSGSGSGVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK*"
     misc_feature    1335..1718
                     /label="MA p15"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1719..1973
                     /label="RBPP p12"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1812..1823
                     /label="PPPY budding motif"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1974..2762
                     /label="CA p30"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2763..2918
                     /label="NC p10"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2919..2939
                     /label="Linker"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2940..3647
                     /label="mCherry"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    3648..3879
                     /label="bGH pA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     promoter        4872..4900
                     /label="AmpR_promoter"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     gene            4942..5802
                     /label="Ampicillin"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             4942..5802
                     /label="Translation 4942-5802"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     rep_origin      5895..6656
                     /label="pBR322_origin"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
ORIGIN
        1 AGCTTGGCCC ATTGCATACG TTGTATCCAT ATCATAATAT GTACATTTAT ATTGGCTCAT
       61 GTCCAACATT ACCGCCATGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA
      121 CGGGGTCATT AGTTCATAGC CCATATTGGA GTTCCGCGTT ACATAACTTA CGGTAAATGG
      181 CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCCTTGACG TCAATAATGA CGTATGTTCC
      241 CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC
      301 TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA
      361 TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC
      421 TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TGTGGCAGTA
      481 CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA
      541 CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA
      601 CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG
      661 AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA
      721 TAGAAGACAC CGGGACCGAT CCAGCCTCCC CTCGAAGCTT TACATGTGGT ACCGAGCTCG
      781 GATCCTGAGA ACTTCAGGGT GAGTCTATGG GACCCTTGAT GTTTTCTTTC CCCTTCTTTT
      841 CTATGGTTAA GTTCATGTCA TAGGAAGGGG AGAAGTAACA GGGTACACAT ATTGACCAAA
      901 TCAGGGTAAT TTTGCATTTG TAATTTTAAA AAATGCTTTC TTCTTTTAAT ATACTTTTTT
      961 GTTTATCTTA TTTCTAATAC TTTCCCTAAT CTCTTTCTTT CAGGGCAATA ATGATACAAT
     1021 GTATCATGCC TCTTTGCACC ATTCTAAAGA ATAACAGTGA TAATTTCTGG GTTAAGGCAA
     1081 TAGCAATATT TCTGCATATA AATATTTCTG CATATAAATT GTAACTGATG TAAGAGGTTT
     1141 CATATTGCTA ATAGCAGCTA CAATCCAGCT ACCATTCTGC TTTTATTTTA TGGTTGGGAT
     1201 AAGGCTGGAT TATTCTGAGT CCAAGCTAGG CCCTTTTGCT AATCATGTTC ATACCTCTTA
     1261 TCTTCCTCCC ACAGCTCCTG GGCAACGTGC TGGTCTGTGT GCTGGCCCAT CACTTTGGCA
     1321 AAGAATTCAT GGGCCAGACT GTTACCACTC CCTTAAGTTT GACCTTAGGT CACTGGAAAG
     1381 ATGTCGAGCG GATCGCTCAC AACCAGTCGG TAGATGTCAA GAAGAGACGT TGGGTTACCT
     1441 TCTGCTCTGC AGAATGGCCA ACCTTTAACG TCGGATGGCC GCGAGACGGC ACCTTTAACC
     1501 GAGACCTCAT CACCCAGGTT AAGATCAAGG TCTTTTCACC TGGCCCGCAT GGACACCCAG
     1561 ACCAGGTCCC CTACATCGTG ACCTGGGAAG CCTTGGCTTT TGACCCCCCT CCCTGGGTCA
     1621 AGCCCTTTGT ACACCCTAAG CCTCCGCCTC CTCTTCCTCC ATCCGCCCCG TCTCTCCCCC
     1681 TTGAACCTCC TCGTTCGACC CCGCCTCGAT CCTCCCTTTA TCCAGCCCTC ACTCCTTCTC
     1741 TAGGCGCCAA ACCTAAACCT CAAGTTCTTT CTGACAGTGG GGGGCCGCTC ATCGACCTAC
     1801 TTACAGAAGA CCCCCCGCCT TATAGGGACC CAAGACCACC CCCTTCCGAC AGGGACGGAA
     1861 ATGGTGGAGA AGCGACCCCT GCGGGAGAGG CACCGGACCC CTCCCCAATG GCATCTCGCC
     1921 TACGTGGGAG ACGGGAGCCC CCTGTGGCCG ACTCCACTAC CTCGCAGGCA TTCCCCCTCC
     1981 GCGCAGGAGG AAACGGACAG CTTCAATACT GGCCGTTCTC CTCTTCTGAC CTTTACAACT
     2041 GGAAAAATAA TAACCCTTCT TTTTCTGAAG ATCCAGGTAA ACTGACAGCT CTGATCGAGT
     2101 CTGTTCTCAT CACCCATCAG CCCACCTGGG ACGACTGTCA GCAGCTGTTG GGGACTCTGC
     2161 TGACCGGAGA AGAAAAACAA CGGGTGCTCT TAGAGGCTAG AAAGGCGGTG CGGGGCGATG
     2221 ATGGGCGCCC CACTCAACTG CCCAATGAAG TCGATGCCGC TTTTCCCCTC GAGCGCCCAG
     2281 ACTGGGATTA CACCACCCAG GCAGGTAGGA ACCACCTAGT CCACTATCGC CAGTTGCTCC
     2341 TAGCGGGTCT CCAAAACGCG GGCAGAAGCC CCACCAATTT GGCCAAGGTA AAAGGAATAA
     2401 CACAAGGGCC CAATGAGTCT CCCTCGGCCT TCCTAGAGAG ACTTAAGGAA GCCTATCGCA
     2461 GGTACACTCC TTATGACCCT GAGGACCCAG GGCAAGAAAC TAATGTGTCT ATGTCTTTCA
     2521 TTTGGCAGTC TGCCCCAGAC ATTGGGAGAA AGTTAGAGAG GTTAGAAGAT TTAAAAAACA
     2581 AGACGCTTGG AGATTTGGTT AGAGAGGCAG AAAAGATCTT TAATAAACGA GAAACCCCGG
     2641 AAGAAAGAGA GGAACGTATC AGGAGAGAAA CAGAGGAAAA AGAAGAACGC CGTAGGACAG
     2701 AGGATGAGCA GAAAGAGAAA GAAAGAGATC GTAGGAGACA TAGAGAGATG AGCAAGCTAT
     2761 TGGCCACTGT CGTTAGTGGA CAGAAACAGG ATAGACAGGG AGGAGAACGA AGGAGGTCCC
     2821 AACTCGATCG CGACCAGTGT GCCTACTGCA AAGAAAAGGG GCACTGGGCT AAAGATTGTC
     2881 CCAAGAAACC ACGAGGACCT CGGGGACCAA GACCGCAGGG ATCAGGATCA GGATCAGGAG
     2941 TGTCCAAGGG CGAGGAAGAT AACATGGCCA TCATCAAGGA GTTCATGAGG TTTAAGGTCC
     3001 ACATGGAGGG TTCAGTCAAT GGCCACGAGT TCGAGATTGA AGGCGAGGGC GAGGGCCGCC
     3061 CCTACGAAGG GACACAGACG GCGAAATTGA AGGTGACCAA AGGCGGGCCA TTGCCCTTCG
     3121 CATGGGACAT CTTGTCCCCT CAGTTTATGT ATGGCAGCAA GGCCTATGTT AAGCACCCCG
     3181 CTGATATCCC GGACTACTTG AAGCTGTCCT TTCCAGAGGG GTTTAAATGG GAGCGCGTTA
     3241 TGAATTTCGA AGACGGAGGA GTGGTTACGG TGACGCAGGA CTCATCCCTG CAGGACGGAG
     3301 AATTTATATA TAAGGTTAAG TTGAGAGGCA CAAACTTCCC AAGCGACGGC CCTGTGATGC
     3361 AGAAGAAAAC AATGGGGTGG GAAGCTTCCA GCGAGCGCAT GTACCCCGAA GATGGCGCCC
     3421 TCAAGGGCGA GATAAAGCAA AGGCTGAAAC TTAAGGACGG CGGTCATTAC GACGCGGAGG
     3481 TCAAGACAAC TTACAAGGCT AAAAAACCCG TTCAGTTGCC TGGGGCTTAC AATGTTAATA
     3541 TCAAACTTGA CATCACAAGC CACAATGAAG ACTATACGAT CGTGGAGCAG TATGAACGAG
     3601 CGGAAGGCAG GCACTCAACG GGGGGGATGG ACGAGCTTTA CAAGTAACTA GAGCTCGCTG
     3661 ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGCC
     3721 TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG AGGAAATTGC
     3781 ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT GGGGTGGGGC AGGACAGCAA
     3841 GGGGGAGGAT TGGGAAGAgA ATAGCAGGCA TGCTGGGGAC ATGGCGCGTT CAACGCTCTC
     3901 AAAACCCCTT AAAAATAAGG TTAACCCGCG AGGCCCCCTA AGAATTCACC CCACCAGTGC
     3961 AGGCTGCCTA TCAGAAAGTG GTGGCTGGTG TGGCTAATGC CCTGGCCCAC AAGTATCACT
     4021 AAGCTCGCTT TCTTGCTGTC CAATTTCTAT TAAAGGTTCC TTTGTTCCCT AAGTCCAACT
     4081 ACTAAACTGG GGGATATTAT GAAGGGCCTT GAGCATCTGG ATTCTGCCTA ATAAAAAACA
     4141 TTTATTTTCA TTGCAATGAT GTATTTAAAT TATTTCTGAA TATTTTACTA AAAAGGGAAT
     4201 GTGGGAGGTC AGTGCATTTA AAACATAAAG AAATGAAGAG CTAGTTCAAA CCTTGGGAAA
     4261 ATACACTATA TCTTAAACTC CATGAAAGAA GGTGAGGCTG CAAACAGCTA ATGCACATTG
     4321 GCAACAGCCC TGATGCCTAT GCCTTATTCA TCCCTCAGAA AAGGATTCAA GTAGAGGCTT
     4381 GATTTGGAGG TTAAAGTTTT GCTATGCTGT ATTTTACATT ACTTATTGTT TTAGCTGTCC
     4441 TCATGAATGT CTTTTCACTA CCCATTTGCT TATCCTGCAT CTCTCAGCCT TGACTCCACT
     4501 CAGTTCTCTT GCTTAGAGAT ACCACCTTTC CCCTGAAGTG TTCCTTCCAT GTTTTACGGC
     4561 GAAGATGGTT TCTCCTCGCC TGGCCACTCA GCCTTAGTTG TCTCTGTTGT CTTATAGAGG
     4621 TCTACTTGAA GAAGGAAAAA CAGGGGGCAT GGTTTGACTG TCCTGTGAGC CCTTCTTCCC
     4681 TGCCTCCCCC ACTCACAGTG ACCCGGAATC CCTCGACATG GCAGTCTAGA TCATTCTTGA
     4741 AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT
     4801 TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT
     4861 TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA
     4921 TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT
     4981 TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT
     5041 GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG
     5101 ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG
     5161 CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA
     5221 CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT
     5281 GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC
     5341 AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG
     5401 GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC
     5461 GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT
     5521 GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA
     5581 GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT
     5641 GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC
     5701 TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA
     5761 CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC
     5821 TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG
     5881 ATCCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC
     5941 GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC
     6001 AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC
     6061 GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC
     6121 GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG
     6181 AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA
     6241 CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA
     6301 TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC
     6361 CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG
     6421 ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGGATGCG CCGCGTGCGG
     6481 CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA
     6541 GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC
     6601 CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA
     6661 GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG
     6721 AGGCGGCATA AATCCCCGTG ACGATCAGCG GTCCAATGAT CGAAGTTAGG CTGGTAAGAG
     6781 CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG
     6841 CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA
     6901 TCCAGCCTCG CGTCGGGGAG CTTTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC
     6961 TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT
     7021 AGTCAGCCAT G
//