LOCUS       pFLAG-VSVG_(pVSVG-FLAG) 7710 bp ds-DNA     circular     15-NOV-2023
DEFINITION  .
KEYWORDS    "accession:addgene_80606_157302"
FEATURES             Location/Qualifiers
     promoter        236..852
                     /label="CMV_immearly_promoter"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    315..602
                     /label="CAG_enhancer"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        863..881
                     /label="T7_promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     gene            906..2492
                     /label="VSV-G"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             906..3266
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPSGGDYKDDDDKGGSLGHNQKGNWKNVPSNYHYCPSSSDLNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPSVEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQFINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFRSNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQTSVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLKYFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFPLYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWFSSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGKEFGSGATNFSLLKQAGDVEENPGPVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK*"
     misc_feature    984..1007
                     /label="FLAG Tag"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2454..2486
                     /label="VSVG Tag"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    2493..2558
                     /label="P2A"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2559..3266
                     /label="mCherry"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     terminator      3307..3534
                     /label="bGH_PA_terminator"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3597..3903
                     /label="f1_origin"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(4023..4238)
                     /label="SV40_enhancer"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     promoter        4035..4303
                     /label="SV40_promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    4202..4279
                     /label="SV40_origin"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     gene            4421..5209
                     /label="NeoR/KanR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     terminator      5389..5508
                     /label="SV40_PA_terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     gene            complement(6714..7574)
                     /label="Ampicillin"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(7616..7644)
                     /label="AmpR_promoter"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
ORIGIN
        1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG
       61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG
      121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC
      181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT
      241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA
      301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC
      361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC
      421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT
      481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT
      541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA
      601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG
      661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC
      721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG
      781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA
      841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC
      901 CCACCATGAA GTGCCTTTTG TACTTAGCCT TTTTATTCAT TGGGGTGAAT TGCAAGTTCA
      961 CCATAGTTTT TCCATCCGGA GGAGATTACA AGGATGACGA CGATAAGGGC GGAAGCTTGG
     1021 GACACAACCA AAAAGGAAAC TGGAAAAATG TTCCTTCTAA TTACCATTAT TGCCCGTCAA
     1081 GCTCAGATTT AAATTGGCAT AATGACTTAA TAGGCACAGC CTTACAAGTC AAAATGCCCA
     1141 AGAGTCACAA GGCTATTCAA GCAGACGGTT GGATGTGTCA TGCTTCCAAA TGGGTCACTA
     1201 CTTGTGATTT CCGCTGGTAT GGACCGAAGT ATATAACACA TTCCATCCGA TCCTTCACTC
     1261 CATCTGTAGA ACAATGCAAG GAAAGCATTG AACAAACGAA ACAAGGAACT TGGCTGAATC
     1321 CAGGCTTCCC TCCTCAAAGT TGTGGATATG CAACTGTGAC GGATGCCGAA GCAGTGATTG
     1381 TCCAGGTGAC TCCTCACCAT GTGCTGGTTG ATGAATACAC AGGAGAATGG GTTGATTCAC
     1441 AGTTCATCAA CGGAAAATGC AGCAATTACA TATGCCCCAC TGTCCATAAC TCTACAACCT
     1501 GGCATTCTGA CTATAAGGTC AAAGGGCTAT GTGATTCTAA CCTCATTTCC ATGGACATCA
     1561 CCTTCTTCTC AGAGGACGGA GAGCTATCAT CCCTGGGAAA GGAGGGCACA GGGTTCAGAA
     1621 GTAACTACTT TGCTTATGAA ACTGGAGGCA AGGCCTGCAA AATGCAATAC TGCAAGCATT
     1681 GGGGAGTCAG ACTCCCATCA GGTGTCTGGT TCGAGATGGC TGATAAGGAT CTCTTTGCTG
     1741 CAGCCAGATT CCCTGAATGC CCAGAAGGGT CAAGTATCTC TGCTCCATCT CAGACCTCAG
     1801 TGGATGTAAG TCTAATTCAG GACGTTGAGA GGATCTTGGA TTATTCCCTC TGCCAAGAAA
     1861 CCTGGAGCAA AATCAGAGCG GGTCTTCCAA TCTCTCCAGT GGATCTCAGC TATCTTGCTC
     1921 CTAAAAACCC AGGAACCGGT CCTGCTTTCA CCATAATCAA TGGTACCCTA AAATACTTTG
     1981 AGACCAGATA CATCAGAGTC GATATTGCTG CTCCAATCCT CTCAAGAATG GTCGGAATGA
     2041 TCAGTGGAAC TACCACAGAA AGGGAACTGT GGGATGACTG GGCACCATAT GAAGACGTGG
     2101 AAATTGGACC CAATGGAGTT CTGAGGACCA GTTCAGGATA TAAGTTTCCT TTATACATGA
     2161 TTGGACATGG TATGTTGGAC TCCGATCTTC ATCTTAGCTC AAAGGCTCAG GTGTTCGAAC
     2221 ATCCTCACAT TCAAGACGCT GCTTCGCAAC TTCCTGATGA TGAGAGTTTA TTTTTTGGTG
     2281 ATACTGGGCT ATCCAAAAAT CCAATCGAGC TTGTAGAAGG TTGGTTCAGT AGTTGGAAAA
     2341 GCTCTATTGC CTCTTTTTTC TTTATCATAG GGTTAATCAT TGGACTATTC TTGGTTCTCC
     2401 GAGTTGGTAT CCATCTTTGC ATTAAATTAA AGCACACCAA GAAAAGACAG ATTTATACAG
     2461 ACATAGAGAT GAACCGACTT GGAAAGGAAT TCGGATCCGG CGCAACAAAC TTCTCTCTGC
     2521 TGAAACAAGC CGGAGATGTC GAAGAGAATC CTGGACCGGT GTCCAAGGGC GAGGAAGATA
     2581 ACATGGCCAT CATCAAGGAG TTCATGAGGT TTAAGGTCCA CATGGAGGGT TCAGTCAATG
     2641 GCCACGAGTT CGAGATTGAA GGCGAGGGCG AGGGCCGCCC CTACGAAGGG ACACAGACGG
     2701 CGAAATTGAA GGTGACCAAA GGCGGGCCAT TGCCCTTCGC ATGGGACATC TTGTCCCCTC
     2761 AGTTTATGTA TGGCAGCAAG GCCTATGTTA AGCACCCCGC TGATATCCCG GACTACTTGA
     2821 AGCTGTCCTT TCCAGAGGGG TTTAAATGGG AGCGCGTTAT GAATTTCGAA GACGGAGGAG
     2881 TGGTTACGGT GACGCAGGAC TCATCCCTGC AGGACGGAGA ATTTATATAT AAGGTTAAGT
     2941 TGAGAGGCAC AAACTTCCCA AGCGACGGCC CTGTGATGCA GAAGAAAACA ATGGGGTGGG
     3001 AAGCTTCCAG CGAGCGCATG TACCCCGAAG ATGGCGCCCT CAAGGGCGAG ATAAAGCAAA
     3061 GGCTGAAACT TAAGGACGGC GGTCATTACG ACGCGGAGGT CAAGACAACT TACAAGGCTA
     3121 AAAAACCCGT TCAGTTGCCT GGGGCTTACA ATGTTAATAT CAAACTTGAC ATCACAAGCC
     3181 ACAATGAAGA CTATACGATC GTGGAGCAGT ATGAACGAGC GGAAGGCAGG CACTCAACGG
     3241 GGGGGATGGA CGAGCTTTAC AAGTAACTCG AGTCTAGAGG GCCCGTTTAA ACCCGCTGAT
     3301 CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT
     3361 CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT
     3421 CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG
     3481 GGGAGGATTG GGAAGACAAT AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGCTTCTG
     3541 AGGCGGAAAG AACCAGCTGG GGCTCTAGGG GGTATCCCCA CGCGCCCTGT AGCGGCGCAT
     3601 TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG
     3661 CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC
     3721 AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC
     3781 CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT
     3841 TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA
     3901 CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG
     3961 CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTAA TTCTGTGGAA
     4021 TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG
     4081 CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG
     4141 AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC
     4201 CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT
     4261 TTTTATTTAT GCAGAGGCCG AGGCCGCCTC TGCCTCTGAG CTATTCCAGA AGTAGTGAGG
     4321 AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT
     4381 CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA
     4441 CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC
     4501 AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT
     4561 TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC
     4621 GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG
     4681 AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC
     4741 TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC
     4801 GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT
     4861 GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC
     4921 CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA
     4981 TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA
     5041 CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT
     5101 TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC
     5161 TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT
     5221 CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC
     5281 ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG
     5341 ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA
     5401 GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT
     5461 TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA
     5521 CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT
     5581 TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG
     5641 GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG
     5701 TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT
     5761 TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG
     5821 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG
     5881 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG
     5941 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA
     6001 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT
     6061 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC
     6121 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG
     6181 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC
     6241 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA
     6301 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG
     6361 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT
     6421 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC
     6481 ACCGCTGGTA GCGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT
     6541 CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT
     6601 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA
     6661 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA
     6721 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC
     6781 TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT
     6841 GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA
     6901 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT
     6961 AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT
     7021 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC
     7081 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC
     7141 TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT
     7201 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT
     7261 GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC
     7321 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT
     7381 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG
     7441 ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT
     7501 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA
     7561 TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT
     7621 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC
     7681 ACATTTCCCC GAAAAGTGCC ACCTGACGTC
//