LOCUS       pPB_EF1a_HA-tNGFR_PuroR 8910 bp ds-DNA     circular     22-NOV-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    123..363
                     /label="PB"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    127..189
                     /label="piggyBac right (3') inverted repeat"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    367..595
                     /label="Chicken Insulator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    665..1194
                     /label="CBX3 (approx) UCOE"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     promoter        1356..2534
                     /label="EF-1-alpha promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1375..1586
                     /label="EF1a core promoter"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     intron          1587..2525
                     /label="EF-1-alpha intron A"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2541..2550
                     /label="kozak"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2547..3413
                     /label="tNGFR"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             2547..4079
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MGAGATGRAMDGPRLLLLLLLGVSLGGAKEACGGGYPYDVPDYAGGGPTGLYTHSGECCKACNLGEGVAQPCGANQTVCEPCLDSVTFSDVVSATEPCKPCTECVGLQSMSAPCVEADDAVCHCAYGYYQDETTGRCEACRVCEAGSGLVFSCQDKQNTVCEECPDGTYSDEANHVDPCLPCTVCEDTERQLRECTRWADAECEEIPGRWITRSTPPEGSDSTAPSTQEPEAPPEQDLIASTVAGVVTTVMGSSQPVVTRGTTDNLIPVYCSILAAVVVGLVAYIAFKRGSGATNFSLLKQAGDVEENPGPMTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     misc_feature    2652..2678
                     /label="HA Tag"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    3414..3479
                     /label="P2A"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             3480..4079
                     /label="PuroR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_RNA        4095..4683
                     /label="WPRE"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     polyA_signal    4733..4854
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(5113..5341)
                     /label="Chicken Insulator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(5343..5655)
                     /label="PB"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     repeat_region   complement(5617..5651)
                     /label="piggyBac left (5') inverted repeat"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     rep_origin      complement(6544..7132)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(7303..8163)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(7303..8163)
                     /label="Translation 8163-7303"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(8164..8268)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     rep_origin      complement(8294..8749)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 cgcgcctcgt tcattcacgt ttttgaaccc gtggaggacg ggcagactcg cggtgcaaat
       61 gtgttttaca gcgtgatgga gcagatgaag atgctcgaca cgctgcagaa cacgcagcta
      121 gaTTAACCCT AGAAAGATAA TCATATTGTG ACGTACGTTA AAGATAATCA TGTGTAAAAT
      181 TGACGCATGT GTTTTATCGG TCTGTATATC GAGGTTTATT TATTAATTTG AATAGATATT
      241 AAGTTTTATT ATATTTACAC TTACATACTA ATAATAAATT CAACAAACAA TTTATTTATG
      301 TTTATTTATT TATTAAAAAA AACAAAAACT CAAAATTTCT TCTATAAAGT AACAAAACTT
      361 TTAtgaGGGA CAGCCCCCCC CCAAAGCCCC CAGGGATGTA ATTACGTCCC TCCCCCGCTA
      421 GGGGGCAGCA GCGAGCCGCC CGGGGCTCCG CTCCGGTCCG GCGCTCCCCC CGCATCCCCG
      481 AGCCGGCAGC GTGCGGGGAC AGCCCGGGCA CGGGGAAGGT GGCACGGGAT CGCTTTCCTC
      541 TGAACGCTTC TCGCTGCTCT TTGAGCCTGC AGACACCTGG GGGGATACGG GGAAAaggcc
      601 tccaccataa agatggttaa ttaacccacc caagatctgg atcgataagc ttgatatcga
      661 attcgggagg tggtccctgc agttacgcca atgataaccc ccgccagaaa aatcttagta
      721 gccttccctt tttgttttcc gtgccccaac tcggcggatt gactcggccc cttccggaaa
      781 cacccgaatc aacttctagt caaattattg ttcacgccgc aatgacccac ccctggcccg
      841 cgtctgtgga actgacccct ggtgtacagg agagttcgct gctgaaagtg gtcccaaagg
      901 ggtactagtt tttaagctcc caactccccc tcccccagcg tctggaggat tccacaccct
      961 cgcaccgcag gggcgaggaa gtgggcggag tccggttttg gcgccagccg ctgaggctgc
     1021 caagcagaaa agccaccgct gaggagactc cggtcactgt cctcgccccg cctccccctt
     1081 ccctcccctt ggggaccacc gggcgccacg ccgcgaacgg taagtgccgc ggtcgtcggc
     1141 gcctccgccc tccccctagg gccccaattc ccagcgggcg cggcgcgcgg ccccgcgcgc
     1201 agctcccggc tccctccccc ttcggatgtg gcttgagctg taggcgcgga gtcctgcagc
     1261 cccgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac
     1321 ctgtaggttt ggcaatcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
     1381 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgatccggtg
     1441 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
     1501 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
     1561 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
     1621 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
     1681 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
     1741 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
     1801 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
     1861 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
     1921 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
     1981 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
     2041 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
     2101 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
     2161 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
     2221 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     2281 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     2341 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     2401 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     2461 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     2521 ttcaggtgtc gtgacgtacg gccaccatgg gggcaggtgc caccggccgc gccatggacg
     2581 ggccgcgcct gctgctgttg ctgcttctgg gggtgtccct tggaggtgcc aaggaggcat
     2641 gcgggggagg gTACCCATAC GATGTTCCAG ATTACGCTgg aggaggcccc acaggcctgt
     2701 acacacacag cggtgagtgc tgcaaagcct gcaacctggg cgagggtgtg gcccagcctt
     2761 gtggagccaa ccagaccgtg tgtgagccct gcctggacag cgtgacgttc tccgacgtgg
     2821 tgagcgcgac cgagccgtgc aagccgtgca ccgagtgcgt ggggctccag agcatgtcgg
     2881 cgccgtgcgt ggaggccgac gacgccgtgt gccactgcgc ctacggctac taccaggatg
     2941 agacgactgg gcgctgcgag gcgtgccgcg tgtgcgaggc gggctcgggc ctcgtgttct
     3001 cctgccagga caagcagaac accgtgtgcg aggagtgccc cgacggcacg tattccgacg
     3061 aggccaacca cgtggacccg tgcctgccct gcaccgtgtg cgaggacacc gagcgccagc
     3121 tccgcgagtg cacacgctgg gccgacgccg agtgcgagga gatccctggc cgttggatta
     3181 cacggtccac acccccagag ggctcggaca gcacagcccc cagcacccag gagcctgagg
     3241 cacctccaga acaagacctc atagccagca cggtggcagg tgtggtgacc acagtgatgg
     3301 gcagctccca gcccgtggtg acccgaggca ccaccgacaa cctcatccct gtctattgct
     3361 ccatcctggc tgctgtggtt gtgggccttg tggcctacat agccttcaag aggGGATCCG
     3421 GCGCAACAAA CTTCTCTCTG CTCAAACAAG CCGGCGACGT CGAAGAGAAT CCTGGGCCGA
     3481 TGACCGAGTA CAAGCCCACG GTGCGCCTCG CCACCCGCGA CGACGTCCCC AGGGCCGTAC
     3541 GCACCCTCGC CGCCGCGTTC GCCGACTACC CCGCCACGCG CCACACCGTC GATCCGGACC
     3601 GCCACATCGA GCGGGTCACC GAGCTGCAAG AACTCTTCCT CACGCGCGTC GGGCTCGACA
     3661 TCGGCAAGGT GTGGGTCGCG GACGACGGCG CCGCGGTGGC GGTCTGGACC ACGCCGGAGA
     3721 GCGTCGAAGC GGGGGCGGTG TTCGCCGAGA TCGGCCCGCG CATGGCCGAG TTGAGCGGTT
     3781 CCCGGCTGGC CGCGCAGCAA CAGATGGAAG GCCTCCTGGC GCCGCACCGG CCCAAGGAGC
     3841 CCGCGTGGTT CCTGGCCACC GTCGGCGTCT CGCCCGACCA CCAGGGCAAG GGTCTGGGCA
     3901 GCGCCGTCGT GCTCCCCGGA GTGGAGGCGG CCGAGCGCGC CGGGGTGCCC GCCTTCCTGG
     3961 AGACCTCCGC GCCCCGCAAC CTCCCCTTCT ACGAGCGGCT CGGCTTCACC GTCACCGCCG
     4021 ACGTCGAGGT GCCCGAAGGA CCGCGCACCT GGTGCATGAC CCGCAAGCCC GGTGCCTAAA
     4081 CGCGTTAAGT CGACaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     4141 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     4201 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     4261 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     4321 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     4381 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     4441 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     4501 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     4561 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     4621 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     4681 cgcCTCGAGT CTAGAGGGCC CGTTTAAACC CGCTGATCAG CCTaactaac taaacttgtt
     4741 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     4801 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     4861 ctggaattga ctcaaatgat gtcaattagt ctatcagaag ctcatctggt ctcccttccg
     4921 ggggacaaga catccctgtt taatatttaa acagcagtgt tcccaaactg ggttcttata
     4981 tcccttgctc tggtcaacca ggttgcaggg tttcctgtcc tcacaggaac gaagtcccta
     5041 aagaaacagt ggcagccagg tttagccccg gaattgactg gattcctttt ttagggccca
     5101 ttggtatggc ttTTTCCCCG TATCCCCCCA GGTGTCTGCA GGCTCAAAGA GCAGCGAGAA
     5161 GCGTTCAGAG GAAAGCGATC CCGTGCCACC TTCCCCGTGC CCGGGCTGTC CCCGCACGCT
     5221 GCCGGCTCGG GGATGCGGGG GGAGCGCCGG ACCGGAGCGG AGCCCCGGGC GGCTCGCTGC
     5281 TGCCCCCTAG CGGGGGAGGG ACGTAATTAC ATCCCTGGGG GCTTTGGGGG GGGGCTGTCC
     5341 CtGATATCTA TAACAAGAAA ATATATATAT AATAAGTTAT CACGTAAGTA GAACATGAAA
     5401 TAACAATATA ATTATCGTAT GAGTTAAATC TTAAAAGTCA CGTAAAAGAT AATCATGCGT
     5461 CATTTTGACT CACGCGGTCG TTATAGTTCA AAATCAGTGA CACTTACCGC ATTGACAAGC
     5521 ACGCCTCACG GGAGCTCCAA GCGGCGACTG AGATGTCCTA AATGCACAGC GACGGATTCG
     5581 CGCTATTTAG AAAGAGAGAG CAATATTTCA AGAATGcatg cgtcaatttt acgcagacta
     5641 tctttctagg gTTAAtctag ctgcatcagg atcatatcgt cgggtctttt ttccggctca
     5701 gtcatcgccc aagctggcgc tatctgggca tcggggagga agaagcccgt gccttttccc
     5761 gcgaggttga agcggcatgg aaagagtttg ccgaggatga ctgctgctgc attgacgttg
     5821 agcgaaaacg cacgtttacc atgatgattc gggaaggtgt ggccatgcac gcctttaacg
     5881 gtgaactgtt cgttcaggcc acctgggata ccagttcgtc gcggcttttc cggacacagt
     5941 tccggatggt cagcccgaag cgcatcagca acccgaacaa taccggcgac agccggaact
     6001 gccgtgccgg tgtgcagatt aatgacagcg gtgcggcgct gggatattac gtcagcgagg
     6061 acgggtatcc tggctggatg ccgcagaaat ggacatggat accccgtgag ttacccggcg
     6121 ggcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     6181 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     6241 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     6301 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     6361 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     6421 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     6481 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     6541 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     6601 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     6661 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     6721 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     6781 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     6841 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     6901 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     6961 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     7021 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     7081 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     7141 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     7201 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     7261 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     7321 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     7381 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     7441 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     7501 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     7561 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     7621 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     7681 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     7741 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     7801 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     7861 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     7921 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     7981 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     8041 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     8101 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     8161 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     8221 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     8281 aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     8341 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     8401 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     8461 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     8521 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     8581 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     8641 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     8701 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca
     8761 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     8821 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     8881 tcacgacgtt gtaaaacgac ggccagtgag
//