LOCUS       pPB_EF1a_Gag∆MA_(PB03-g 8484 bp ds-DNA     circular     16-NOV-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    123..363
                     /label="PB"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    127..189
                     /label="piggyBac right (3') inverted repeat"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    367..595
                     /label="Chicken Insulator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    665..1194
                     /label="CBX3 (approx) UCOE"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     promoter        1356..2534
                     /label="EF-1-alpha promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1375..1586
                     /label="EF1a core promoter"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     intron          1587..2525
                     /label="EF-1-alpha intron A"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2541..2549
                     /label="kozak"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2547..3494
                     /label="MLV Gag (UniProt ID: P03332)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             2547..4265
                     /label="Translation 2547-4265"
                     /translation="MPLRAGGNGQLQYWPFSSSDLYNWKNNNPSFSEDPGKLTALIESVLITHQPTWDDCQQLLGTLLTGEEKQRVLLEARKAVRGDDGRPTQLPNEVDAAFPLERPDWDYTTQAGRNHLVHYRQLLLAGLQNAGRSPTNLAKVKGITQGPNESPSAFLERLKEAYRRYTPYDPEDPGQETNVSMSFIWQSAPDIGRKLERLEDLKNKTLGDLVREAEKIFNKRETPEEREERIRRETEEKEERRRTEDEQKEKERDRRRHREMSKLLATVVSGQKQDRQGGERRRSQLDRDQCAYCKEKGHWAKDCPKKPRGPRGPRPQGSGATNFSLLKQAGDVEENPGPVSKGEEDNASLPATHELHIFGSINGVDFDLVGQGTGNPNDGYEELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDGASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVLTNSLTAADWCRSKKTYPNDKTIISTFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQKAFTDVMGMDELYK*"
     misc_feature    2550..3338
                     /label="CA p30"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    3339..3494
                     /label="NC p10"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    3495..3560
                     /label="P2A"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3561..4265
                     /label="mNeon"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     polyA_signal    4307..4428
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(4687..4915)
                     /label="Chicken Insulator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(4917..5229)
                     /label="PB"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     repeat_region   complement(5191..5225)
                     /label="piggyBac left (5') inverted repeat"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     rep_origin      complement(6118..6706)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(6877..7737)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(6877..7737)
                     /label="Translation 7737-6877"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(7738..7842)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     rep_origin      complement(7868..8323)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 cgcgcctcgt tcattcacgt ttttgaaccc gtggaggacg ggcagactcg cggtgcaaat
       61 gtgttttaca gcgtgatgga gcagatgaag atgctcgaca cgctgcagaa cacgcagcta
      121 gaTTAACCCT AGAAAGATAA TCATATTGTG ACGTACGTTA AAGATAATCA TGTGTAAAAT
      181 TGACGCATGT GTTTTATCGG TCTGTATATC GAGGTTTATT TATTAATTTG AATAGATATT
      241 AAGTTTTATT ATATTTACAC TTACATACTA ATAATAAATT CAACAAACAA TTTATTTATG
      301 TTTATTTATT TATTAAAAAA AACAAAAACT CAAAATTTCT TCTATAAAGT AACAAAACTT
      361 TTAtgaGGGA CAGCCCCCCC CCAAAGCCCC CAGGGATGTA ATTACGTCCC TCCCCCGCTA
      421 GGGGGCAGCA GCGAGCCGCC CGGGGCTCCG CTCCGGTCCG GCGCTCCCCC CGCATCCCCG
      481 AGCCGGCAGC GTGCGGGGAC AGCCCGGGCA CGGGGAAGGT GGCACGGGAT CGCTTTCCTC
      541 TGAACGCTTC TCGCTGCTCT TTGAGCCTGC AGACACCTGG GGGGATACGG GGAAAaggcc
      601 tccaccataa agatggttaa ttaacccacc caagatctgg atcgataagc ttgatatcga
      661 attcgggagg tggtccctgc agttacgcca atgataaccc ccgccagaaa aatcttagta
      721 gccttccctt tttgttttcc gtgccccaac tcggcggatt gactcggccc cttccggaaa
      781 cacccgaatc aacttctagt caaattattg ttcacgccgc aatgacccac ccctggcccg
      841 cgtctgtgga actgacccct ggtgtacagg agagttcgct gctgaaagtg gtcccaaagg
      901 ggtactagtt tttaagctcc caactccccc tcccccagcg tctggaggat tccacaccct
      961 cgcaccgcag gggcgaggaa gtgggcggag tccggttttg gcgccagccg ctgaggctgc
     1021 caagcagaaa agccaccgct gaggagactc cggtcactgt cctcgccccg cctccccctt
     1081 ccctcccctt ggggaccacc gggcgccacg ccgcgaacgg taagtgccgc ggtcgtcggc
     1141 gcctccgccc tccccctagg gccccaattc ccagcgggcg cggcgcgcgg ccccgcgcgc
     1201 agctcccggc tccctccccc ttcggatgtg gcttgagctg taggcgcgga gtcctgcagc
     1261 cccgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac
     1321 ctgtaggttt ggcaatcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
     1381 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgatccggtg
     1441 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
     1501 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
     1561 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
     1621 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
     1681 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
     1741 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
     1801 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
     1861 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
     1921 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
     1981 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
     2041 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
     2101 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
     2161 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
     2221 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     2281 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     2341 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     2401 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     2461 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     2521 ttcaggtgtc gtgacgtacg gccaccATGC CCCTCCGCGC AGGAGGAAAC GGACAGCTTC
     2581 AATACTGGCC GTTCTCCTCT TCTGACCTTT ACAACTGGAA AAATAATAAC CCTTCTTTTT
     2641 CTGAAGATCC AGGTAAACTG ACAGCTCTGA TCGAGTCTGT TCTCATCACC CATCAGCCCA
     2701 CCTGGGACGA CTGTCAGCAG CTGTTGGGGA CTCTGCTGAC CGGAGAAGAA AAACAACGGG
     2761 TGCTCTTAGA GGCTAGAAAG GCGGTGCGGG GCGATGATGG GCGCCCCACT CAACTGCCCA
     2821 ATGAAGTCGA TGCCGCTTTT CCCCTCGAGC GCCCAGACTG GGATTACACC ACCCAGGCAG
     2881 GTAGGAACCA CCTAGTCCAC TATCGCCAGT TGCTCCTAGC GGGTCTCCAA AACGCGGGCA
     2941 GAAGCCCCAC CAATTTGGCC AAGGTAAAAG GAATAACACA AGGGCCCAAT GAGTCTCCCT
     3001 CGGCCTTCCT AGAGAGACTT AAGGAAGCCT ATCGCAGGTA CACTCCTTAT GACCCTGAGG
     3061 ACCCAGGGCA AGAAACTAAT GTGTCTATGT CTTTCATTTG GCAGTCTGCC CCAGACATTG
     3121 GGAGAAAGTT AGAGAGGTTA GAAGATTTAA AAAACAAGAC GCTTGGAGAT TTGGTTAGAG
     3181 AGGCAGAAAA GATCTTTAAT AAACGAGAAA CCCCGGAAGA AAGAGAGGAA CGTATCAGGA
     3241 GAGAAACAGA GGAAAAAGAA GAACGCCGTA GGACAGAGGA TGAGCAGAAA GAGAAAGAAA
     3301 GAGATCGTAG GAGACATAGA GAGATGAGCA AGCTATTGGC CACTGTCGTT AGTGGACAGA
     3361 AACAGGATAG ACAGGGAGGA GAACGAAGGA GGTCCCAACT CGATCGCGAC CAGTGTGCCT
     3421 ACTGCAAAGA AAAGGGGCAC TGGGCTAAAG ATTGTCCCAA GAAACCACGA GGACCTCGGG
     3481 GACCAAGACC GCAGGGATCC GGCGCAACAA ACTTCTCTCT GCTGAAACAA GCCGGAGATG
     3541 TCGAAGAGAA TCCTGGACCG GTCAGCAAGG GCGAGGAGGA CAACGCCTCT CTCCCAGCGA
     3601 CACACGAGTT ACACATCTTT GGCTCCATCA ACGGTGTGGA CTTCGACCTC GTGGGTCAGG
     3661 GCACCGGCAA TCCAAACGAC GGTTACGAGG AGCTCAACCT CAAGTCCACC AAGGGCGACC
     3721 TCCAGTTCTC CCCCTGGATT CTGGTCCCTC ATATCGGGTA CGGCTTCCAT CAGTACCTGC
     3781 CCTACCCAGA CGGGATGTCG CCTTTCCAGG CCGCCATGGT GGACGGCTCC GGATACCAAG
     3841 TCCATCGCAC AATGCAGTTC GAAGACGGTG CCTCCCTTAC TGTCAACTAC CGCTACACCT
     3901 ACGAGGGAAG CCACATCAAA GGAGAGGCCC AGGTCAAGGG GACTGGTTTC CCTGCCGACG
     3961 GTCCTGTCCT CACCAACTCG CTCACCGCTG CGGACTGGTG CAGGTCGAAG AAGACTTACC
     4021 CCAACGACAA AACCATCATC AGTACCTTCA AGTGGAGTTA CACCACTGGA AACGGCAAGC
     4081 GCTACCGGAG CACTGCGCGG ACCACCTACA CCTTTGCCAA GCCAATGGCG GCCAACTATC
     4141 TCAAGAACCA GCCGATGTAC GTGTTCCGCA AGACGGAGCT CAAGCACTCC AAGACCGAGC
     4201 TCAACTTCAA GGAGTGGCAA AAGGCCTTTA CCGACGTCAT GGGCATGGAC GAGCTCTACA
     4261 AGtaacaatc aacctctgga ttacaaaatt tgtggttaac taactaaact tgtttattgc
     4321 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     4381 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggaa
     4441 ttgactcaaa tgatgtcaat tagtctatca gaagctcatc tggtctccct tccgggggac
     4501 aagacatccc tgtttaatat ttaaacagca gtgttcccaa actgggttct tatatccctt
     4561 gctctggtca accaggttgc agggtttcct gtcctcacag gaacgaagtc cctaaagaaa
     4621 cagtggcagc caggtttagc cccggaattg actggattcc ttttttaggg cccattggta
     4681 tggcttTTTC CCCGTATCCC CCCAGGTGTC TGCAGGCTCA AAGAGCAGCG AGAAGCGTTC
     4741 AGAGGAAAGC GATCCCGTGC CACCTTCCCC GTGCCCGGGC TGTCCCCGCA CGCTGCCGGC
     4801 TCGGGGATGC GGGGGGAGCG CCGGACCGGA GCGGAGCCCC GGGCGGCTCG CTGCTGCCCC
     4861 CTAGCGGGGG AGGGACGTAA TTACATCCCT GGGGGCTTTG GGGGGGGGCT GTCCCtGATA
     4921 TCTATAACAA GAAAATATAT ATATAATAAG TTATCACGTA AGTAGAACAT GAAATAACAA
     4981 TATAATTATC GTATGAGTTA AATCTTAAAA GTCACGTAAA AGATAATCAT GCGTCATTTT
     5041 GACTCACGCG GTCGTTATAG TTCAAAATCA GTGACACTTA CCGCATTGAC AAGCACGCCT
     5101 CACGGGAGCT CCAAGCGGCG ACTGAGATGT CCTAAATGCA CAGCGACGGA TTCGCGCTAT
     5161 TTAGAAAGAG AGAGCAATAT TTCAAGAATG catgcgtcaa ttttacgcag actatctttc
     5221 tagggTTAAt ctagctgcat caggatcata tcgtcgggtc ttttttccgg ctcagtcatc
     5281 gcccaagctg gcgctatctg ggcatcgggg aggaagaagc ccgtgccttt tcccgcgagg
     5341 ttgaagcggc atggaaagag tttgccgagg atgactgctg ctgcattgac gttgagcgaa
     5401 aacgcacgtt taccatgatg attcgggaag gtgtggccat gcacgccttt aacggtgaac
     5461 tgttcgttca ggccacctgg gataccagtt cgtcgcggct tttccggaca cagttccgga
     5521 tggtcagccc gaagcgcatc agcaacccga acaataccgg cgacagccgg aactgccgtg
     5581 ccggtgtgca gattaatgac agcggtgcgg cgctgggata ttacgtcagc gaggacgggt
     5641 atcctggctg gatgccgcag aaatggacat ggataccccg tgagttaccc ggcgggcgcg
     5701 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     5761 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     5821 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     5881 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     5941 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     6001 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     6061 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     6121 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     6181 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     6241 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     6301 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     6361 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     6421 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     6481 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     6541 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     6601 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     6661 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     6721 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     6781 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     6841 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     6901 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     6961 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     7021 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     7081 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     7141 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     7201 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     7261 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     7321 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     7381 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     7441 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     7501 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     7561 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     7621 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     7681 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     7741 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     7801 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     7861 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     7921 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     7981 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     8041 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     8101 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     8161 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     8221 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     8281 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg
     8341 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     8401 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     8461 cgttgtaaaa cgacggccag tgag
//