LOCUS       CamKII-4xmitoGC-IRES_M 11689 bp ds-DNA     circular     22-JAN-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    7..12
                     /label="KOZAK"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    7..108
                     /label="Insert1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    7..423
                     /label="Insert MTS longer"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             13..108
                     /label="#1 COX8 sig pept"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             13..1776
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAKIHSLGDPMSVLTPLLLRGLTGSARRLPVPRAKIHSLGDPMSVLTPLLLRGLTGSARRLPVPRAKIHSLGDPMSVLTPLLLRGLTGSARRLPVPRAKIHSLGDPRSGSAKDPTMGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNKTGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEEFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK*"
     CDS             109..204
                     /label="#2 COX8 sig pept"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             205..300
                     /label="#3 COX8 sig pept"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             301..396
                     /label="#4 COX8 sig pept"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             397..423
                     /label="linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             397..423
                     /label="Translation 397-423"
                     /translation="RSGSAKDPT"
     CDS             424..1773
                     /label="GCaMP6f"
                     /ApEinfo_revcolor="#339966"
                     /ApEinfo_fwdcolor="#339966"
     CDS             433..450
                     /label="6xHis"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             490..498
                     /label="Xpress™ tag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             499..513
                     /label="Xpress™ tag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1815..2402
                     /label="IRES2"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_fwdcolor="#800080"
                     /locus_tag="IRES2"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    2409..2414
                     /label="Kozak sequence"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             2415..2426
                     /label="Translation 2415-2426"
                     /translation="RPV*"
     misc_feature    2481..3069
                     /label="WPRE"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(2952..2963)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(3072..3088)
                     /label="KS primer"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     LTR             3594..3774
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal    3806..4030
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     rep_origin      4076..4504
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        4518..4847
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     rep_origin      4698..4833
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        4895..4942
                     /label="EM7 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             4961..5335
                     /label="BleoR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     polyA_signal    5465..5586
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(5635..5651)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     protein_bind    5659..5675
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     promoter        complement(5683..5689)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(5690..5707)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(5708..5713)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    5728..5749
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     rep_origin      complement(6037..6625)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(6796..7587)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(7588..7656)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        complement(7657..7761)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     enhancer        8027..8406
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        8408..8606
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     LTR             8624..8804
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    8851..8976
                     /label="HIV-1 Ψ"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    9469..9702
                     /label="RRE"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    10229..10346
                     /label="cPPT/CTS"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        10448..11648
                     /label="CamKII promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 GCTAGCACCG CCATGAGCGT GCTGACACCT CTGCTGCTGA GAGGCCTGAC AGGCTCTGCT
       61 AGAAGGCTGC CTGTGCCTAG AGCCAAGATC CACTCTCTGG GCGACCCTAT GTCTGTGCTG
      121 ACTCCACTGC TCCTGCGGGG ACTGACTGGA TCTGCTCGTA GACTGCCAGT GCCACGGGCT
      181 AAGATTCACA GCCTGGGCGA TCCAATGAGT GTGCTTACCC CACTCCTGCT CAGAGGACTG
      241 ACCGGAAGTG CTAGACGCCT GCCAGTTCCA AGGGCCAAAA TCCATAGTCT GGGAGATCCC
      301 ATGTCCGTGT TGACACCCCT CTTGCTGAGG GGACTTACCG GCTCAGCTCG GAGACTCCCT
      361 GTTCCAAGAG CAAAGATTCA TTCCCTCGGC GACCCAAGGT CTGGCAGCGC TAAAGACCCT
      421 ACCATGGGTA GCCACCACCA CCATCACCAC GGCATGGCTT CTATGACAGG CGGCCAGCAG
      481 ATGGGCCGCG ACCTGTACGA TGACGACGAT AAGGACCTGG CCACAATGGT GGACAGCAGC
      541 AGGCGGAAGT GGAACAAGAC AGGCCACGCC GTTAGAGCCA TCGGCAGACT GTCTAGCCTG
      601 GAAAACGTGT ACATCAAGGC CGACAAGCAG AAGAACGGCA TCAAGGCTAA CTTCAAGATC
      661 CGGCACAACA TCGAGGACGG CGGAGTGCAG CTGGCTTACC ACTACCAGCA GAACACCCCT
      721 ATCGGCGACG GACCAGTTCT GCTGCCTGAC AACCACTACC TGAGCGTGCA GAGCAAGCTG
      781 AGCAAGGACC CCAACGAGAA GAGGGACCAC ATGGTGCTGC TGGAATTTGT GACCGCCGCT
      841 GGCATCACAC TCGGCATGGA CGAACTGTAC AAAGGCGGCA CAGGCGGCTC TATGGTGTCC
      901 AAGGGCGAAG AACTGTTCAC CGGCGTGGTG CCCATTCTGG TGGAACTGGA TGGGGATGTG
      961 AACGGCCACA AGTTCAGCGT CAGCGGAGAA GGCGAAGGCG ACGCCACATA CGGAAAGCTG
     1021 ACCCTGAAGT TCATCTGCAC CACCGGCAAA CTGCCCGTGC CTTGGCCTAC ACTGGTCACC
     1081 ACACTGACAT ACGGCGTGCA GTGCTTCAGC AGATACCCCG ACCATATGAA GCAGCACGAC
     1141 TTCTTCAAGA GCGCCATGCC TGAGGGCTAC ATCCAAGAGA GAACCATCTT CTTTAAGGAC
     1201 GACGGCAACT ACAAGACCAG GGCCGAAGTG AAGTTCGAGG GCGACACCCT GGTCAACAGA
     1261 ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAACA TCCTGGGCCA CAAGCTGGAG
     1321 TACAACCTGC CTGACCAGCT GACCGAGGAA CAGATCGCCG AGTTCAAAGA AGAGTTCAGC
     1381 CTGTTCGACA AGGACGGCGA CGGCACAATC ACCACCAAAG AACTGGGCAC CGTGATGAGA
     1441 AGCCTGGGAC AGAACCCTAC AGAGGCCGAG CTGCAGGACA TGATCAACGA GGTTGACGCT
     1501 GATGGCGACG GAACTATCGA CTTCCCCGAG TTCCTGACCA TGATGGCCAG AAAGATGAAG
     1561 TACAGGGACA CAGAGGAAGA GATCCGCGAG GCCTTCGGCG TGTTCGATAA GGATGGAAAC
     1621 GGCTACATCT CTGCCGCTGA GCTGAGACAC GTGATGACCA ACCTGGGCGA GAAGCTGACA
     1681 GACGAAGAGG TGGACGAGAT GATCAGAGAG GCCGACATTG ACGGCGACGG GCAAGTGAAC
     1741 TACGAAGAGT TTGTCCAGAT GATGACCGCC AAGTGAGCGG CCGCGGGGGC CCGTTTGGGC
     1801 ATGCTCGAAG ATCCGCCCCT CTCCCTCCCC CCCCCCTAAC GTTACTGGCC GAAGCCGCTT
     1861 GGAATAAGGC CGGTGTGCGT TTGTCTATAT GTTATTTTCC ACCATATTGC CGTCTTTTGG
     1921 CAATGTGAGG GCCCGGAAAC CTGGCCCTGT CTTCTTGACG AGCATTCCTA GGGGTCTTTC
     1981 CCCTCTCGCC AAAGGAATGC AAGGTCTGTT GAATGTCGTG AAGGAAGCAG TTCCTCTGGA
     2041 AGCTTCTTGA AGACAAACAA CGTCTGTAGC GACCCTTTGC AGGCAGCGGA ACCCCCCACC
     2101 TGGCGACAGG TGCCTCTGCG GCCAAAAGCC ACGTGTATAA GATACACCTG CAAAGGCGGC
     2161 ACAACCCCAG TGCCACGTTG TGAGTTGGAT AGTTGTGGAA AGAGTCAAAT GGCTCTCCTC
     2221 AAGCGTATTC AACAAGGGGC TGAAGGATGC CCAGAAGGTA CCCCATTGTA TGGGATCTGA
     2281 TCTGGGGCCT CGGTGCACAT GCTTTACATG TGTTTAGTCG AGGTTAAAAA AACGTCTAGG
     2341 CCCCCCGAAC CACGGGGACG TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA
     2401 CCgGATCCGC CACCCGACCG GTATAAAGAT CTgaattcga tatcTGAAAT CTCGAGTCTA
     2461 GAGATATCaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     2521 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     2581 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     2641 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     2701 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     2761 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     2821 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     2881 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     2941 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     3001 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     3061 cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta
     3121 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     3181 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     3241 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     3301 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga
     3361 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag
     3421 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
     3481 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
     3541 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
     3601 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     3661 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     3721 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
     3781 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     3841 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     3901 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     3961 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     4021 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     4081 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     4141 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     4201 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     4261 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     4321 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     4381 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     4441 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     4501 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     4561 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     4621 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     4681 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     4741 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     4801 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     4861 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
     4921 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
     4981 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
     5041 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
     5101 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
     5161 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
     5221 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
     5281 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
     5341 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     5401 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     5461 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     5521 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     5581 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     5641 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     5701 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     5761 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     5821 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     5881 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     5941 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     6001 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6061 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     6121 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     6181 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     6241 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     6301 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     6361 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     6421 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     6481 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     6541 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     6601 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     6661 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     6721 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     6781 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     6841 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     6901 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     6961 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     7021 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     7081 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     7141 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     7201 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     7261 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     7321 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     7381 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     7441 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     7501 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     7561 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     7621 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     7681 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     7741 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     7801 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
     7861 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
     7921 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
     7981 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
     8041 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     8101 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     8161 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     8221 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     8281 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     8341 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     8401 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     8461 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     8521 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     8581 gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga
     8641 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     8701 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     8761 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     8821 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     8881 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     8941 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     9001 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     9061 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     9121 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     9181 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     9241 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     9301 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     9361 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     9421 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     9481 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     9541 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     9601 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     9661 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     9721 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     9781 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     9841 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     9901 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     9961 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
    10021 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
    10081 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
    10141 gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct
    10201 gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
    10261 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
    10321 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt
    10381 ggttaattaa cattatggcc ttaggtcact tcatctccat ggggttcttc ttctgatttt
    10441 ctagaaaatg agatgggggt gcagagagct tcctcagtga cctgcccagg gtcacatcag
    10501 aaatgtcaga gctagaactt gaactcagat tactaatctt aaattccatg ccttgggggc
    10561 atgcaagtac gatatacaga aggagtgaac tcattagggc agatgaccaa tgagtttagg
    10621 aaagaagagt ccagggcagg gtacatctac accacccgcc cagccctggg tgagtccagc
    10681 cacgttcacc tcattatagt tgcctctctc cagtcctacc ttgacgggaa gcacaagcag
    10741 aaactgggac aggagcccca ggagaccaaa tcttcatggt ccctctggga ggatgggtgg
    10801 ggagagctgt ggcagaggcc tcaggagggg ccctgctgct cagtggtgac agataggggt
    10861 gagaaagcag acagagtcat tccgtcagca ttctgggtct gtttggtact tcttctcacg
    10921 ctaaggtggc ggtgtgatat gcacaatggc taaaaagcag ggagagctgg aaagaaacaa
    10981 ggacagagac agaggccaag tcaaccagac caattcccag aggaagcaaa gaaaccatta
    11041 cagagactac aagggggaag ggaaggagag atgaattagc ttcccctgta aaccttagaa
    11101 cccagctgtt gccagggcaa cggggcaata cctgtctctt cagaggagat gaagttgcca
    11161 gggtaactac atcctgtctt tctcaaggac catcccagaa tgtggcaccc actagccgtt
    11221 accatagcaa ctgcctcttt gccccactta atcccatccc gtctgttaaa agggccctat
    11281 agttggaggt gggggaggta ggaagagcga tgatcacttg tggactaagt ttgttcacat
    11341 ccccttctcc aaccccctca gtacatcacc ctgggagaac aaggtccact tgcttctggg
    11401 cccacacagt cctgcagtat tgtgtatata aggccagggc aacggaggag caggttttga
    11461 agtgaaaggc aggcaggtgt tggggaggca gttaccgggg caacgggaac agggcgtttc
    11521 ggaggtggtt gccatgggga cctggatgct gacgaaggct cgcgaggctg tgagcagcca
    11581 cagtgccctg ctcagaagcc ccaagctcgt caatcaagct ggttctccat ttgcactcag
    11641 gagcacgggc aggcgagtgg cccctagttc tgggggcagc gggGgatcc
//