LOCUS       CamKII-mitoGCaMP6f-IRE 13909 bp ds-DNA     circular     22-JAN-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    7..12
                     /label="KOZAK"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    7..108
                     /label="Insert1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    7..423
                     /label="Insert MTS longer"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             13..108
                     /label="#1 COX8 sig pept"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             109..204
                     /label="#2 COX8 sig pept"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             205..300
                     /label="#3 COX8 sig pept"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             301..396
                     /label="#4 COX8 sig pept"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             397..423
                     /label="linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             424..1773
                     /label="GCaMP6f"
                     /ApEinfo_revcolor="#339966"
                     /ApEinfo_fwdcolor="#339966"
     CDS             433..450
                     /label="6xHis"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             490..498
                     /label="Xpress™ tag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             499..513
                     /label="Xpress™ tag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1815..2402
                     /label="IRES2"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_fwdcolor="#800080"
                     /locus_tag="IRES2"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    2409..2414
                     /label="Kozak sequence"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    2415..4634
                     /label="Letm1 codon optimised"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    4401..4508
                     /label="Ef-hand uniprot"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    4440..4478
                     /label="EF-hand Ca binding domain"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    4701..5289
                     /label="WPRE"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(5172..5183)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(5292..5308)
                     /label="KS primer"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     LTR             5814..5994
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal    6026..6250
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     rep_origin      6296..6724
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        6738..7067
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     rep_origin      6918..7053
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        7115..7162
                     /label="EM7 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             7181..7555
                     /label="BleoR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     polyA_signal    7685..7806
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(7855..7871)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     protein_bind    7879..7895
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     promoter        complement(7903..7909)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(7910..7927)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(7928..7933)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    7948..7969
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     rep_origin      complement(8257..8845)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(9016..9807)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(9808..9876)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        complement(9877..9981)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     enhancer        10247..10626
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        10628..10826
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     LTR             10844..11024
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    11071..11196
                     /label="HIV-1 Ψ"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    11689..11922
                     /label="RRE"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    12449..12566
                     /label="cPPT/CTS"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        12668..13868
                     /label="CamKII promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 GCTAGCACCG CCATGAGCGT GCTGACACCT CTGCTGCTGA GAGGCCTGAC AGGCTCTGCT
       61 AGAAGGCTGC CTGTGCCTAG AGCCAAGATC CACTCTCTGG GCGACCCTAT GTCTGTGCTG
      121 ACTCCACTGC TCCTGCGGGG ACTGACTGGA TCTGCTCGTA GACTGCCAGT GCCACGGGCT
      181 AAGATTCACA GCCTGGGCGA TCCAATGAGT GTGCTTACCC CACTCCTGCT CAGAGGACTG
      241 ACCGGAAGTG CTAGACGCCT GCCAGTTCCA AGGGCCAAAA TCCATAGTCT GGGAGATCCC
      301 ATGTCCGTGT TGACACCCCT CTTGCTGAGG GGACTTACCG GCTCAGCTCG GAGACTCCCT
      361 GTTCCAAGAG CAAAGATTCA TTCCCTCGGC GACCCAAGGT CTGGCAGCGC TAAAGACCCT
      421 ACCATGGGTA GCCACCACCA CCATCACCAC GGCATGGCTT CTATGACAGG CGGCCAGCAG
      481 ATGGGCCGCG ACCTGTACGA TGACGACGAT AAGGACCTGG CCACAATGGT GGACAGCAGC
      541 AGGCGGAAGT GGAACAAGAC AGGCCACGCC GTTAGAGCCA TCGGCAGACT GTCTAGCCTG
      601 GAAAACGTGT ACATCAAGGC CGACAAGCAG AAGAACGGCA TCAAGGCTAA CTTCAAGATC
      661 CGGCACAACA TCGAGGACGG CGGAGTGCAG CTGGCTTACC ACTACCAGCA GAACACCCCT
      721 ATCGGCGACG GACCAGTTCT GCTGCCTGAC AACCACTACC TGAGCGTGCA GAGCAAGCTG
      781 AGCAAGGACC CCAACGAGAA GAGGGACCAC ATGGTGCTGC TGGAATTTGT GACCGCCGCT
      841 GGCATCACAC TCGGCATGGA CGAACTGTAC AAAGGCGGCA CAGGCGGCTC TATGGTGTCC
      901 AAGGGCGAAG AACTGTTCAC CGGCGTGGTG CCCATTCTGG TGGAACTGGA TGGGGATGTG
      961 AACGGCCACA AGTTCAGCGT CAGCGGAGAA GGCGAAGGCG ACGCCACATA CGGAAAGCTG
     1021 ACCCTGAAGT TCATCTGCAC CACCGGCAAA CTGCCCGTGC CTTGGCCTAC ACTGGTCACC
     1081 ACACTGACAT ACGGCGTGCA GTGCTTCAGC AGATACCCCG ACCATATGAA GCAGCACGAC
     1141 TTCTTCAAGA GCGCCATGCC TGAGGGCTAC ATCCAAGAGA GAACCATCTT CTTTAAGGAC
     1201 GACGGCAACT ACAAGACCAG GGCCGAAGTG AAGTTCGAGG GCGACACCCT GGTCAACAGA
     1261 ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAACA TCCTGGGCCA CAAGCTGGAG
     1321 TACAACCTGC CTGACCAGCT GACCGAGGAA CAGATCGCCG AGTTCAAAGA AGAGTTCAGC
     1381 CTGTTCGACA AGGACGGCGA CGGCACAATC ACCACCAAAG AACTGGGCAC CGTGATGAGA
     1441 AGCCTGGGAC AGAACCCTAC AGAGGCCGAG CTGCAGGACA TGATCAACGA GGTTGACGCT
     1501 GATGGCGACG GAACTATCGA CTTCCCCGAG TTCCTGACCA TGATGGCCAG AAAGATGAAG
     1561 TACAGGGACA CAGAGGAAGA GATCCGCGAG GCCTTCGGCG TGTTCGATAA GGATGGAAAC
     1621 GGCTACATCT CTGCCGCTGA GCTGAGACAC GTGATGACCA ACCTGGGCGA GAAGCTGACA
     1681 GACGAAGAGG TGGACGAGAT GATCAGAGAG GCCGACATTG ACGGCGACGG GCAAGTGAAC
     1741 TACGAAGAGT TTGTCCAGAT GATGACCGCC AAGTGAGCGG CCGCGGGGGC CCGTTTGGGC
     1801 ATGCTCGAAG ATCCGCCCCT CTCCCTCCCC CCCCCCTAAC GTTACTGGCC GAAGCCGCTT
     1861 GGAATAAGGC CGGTGTGCGT TTGTCTATAT GTTATTTTCC ACCATATTGC CGTCTTTTGG
     1921 CAATGTGAGG GCCCGGAAAC CTGGCCCTGT CTTCTTGACG AGCATTCCTA GGGGTCTTTC
     1981 CCCTCTCGCC AAAGGAATGC AAGGTCTGTT GAATGTCGTG AAGGAAGCAG TTCCTCTGGA
     2041 AGCTTCTTGA AGACAAACAA CGTCTGTAGC GACCCTTTGC AGGCAGCGGA ACCCCCCACC
     2101 TGGCGACAGG TGCCTCTGCG GCCAAAAGCC ACGTGTATAA GATACACCTG CAAAGGCGGC
     2161 ACAACCCCAG TGCCACGTTG TGAGTTGGAT AGTTGTGGAA AGAGTCAAAT GGCTCTCCTC
     2221 AAGCGTATTC AACAAGGGGC TGAAGGATGC CCAGAAGGTA CCCCATTGTA TGGGATCTGA
     2281 TCTGGGGCCT CGGTGCACAT GCTTTACATG TGTTTAGTCG AGGTTAAAAA AACGTCTAGG
     2341 CCCCCCGAAC CACGGGGACG TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA
     2401 CCgGATCCGC CACCATGGCG AGCATCCTGC TGAGAAGCTG TAGAGGCAGA GGCCCTGCCA
     2461 GACTGGCTTC TCCTAGAGCT GCTTCCCCTA GAGGCAGCCT GAGAGACAGA GCCTGTCTGA
     2521 GCTGTACCAG AACCGTGGGC CTGACCTCTC ACGAGTCTGT GCTGAGCAGA TGCTGCACCC
     2581 CTGCTAACCC CGTGTACCTG TACTTCAAGA GCGAGCCCCT GAGCTGCTGG ACACAGAGGC
     2641 CTGAATGTCA AGGCACAGTG GCCAGAGCCG CTTGGACTCC TGCTTCTGCT AGACTGGTGG
     2701 TCACAGGCCC TCAGTACCTG CCTGTTAGAG GCTGGCACAG CTCTAGCCCT CTGGGCGAAG
     2761 ATAGCATGAT CGAGAAGTCC CTGAAGTCTC TGAAGGACAA GAACAAGAAG CTGGAAGAAG
     2821 GCGGCCCTGT CTACAGCCCT CCTGCTCAGG TGGTGGTCAA GAAAAGCCTG GGCCAGAAGA
     2881 TCCTGGACGA GCTGAAGCAC TACTACCACG GCTTCAGGCT GCTGTGGATC GACACAAAGA
     2941 TCGCCGCCAG AATGCTGTGG CGCATCCTGA ACGGACACAC CCTGACCAGA AGAGAGAGGC
     3001 GGCAGTTCCT GAGAATCTGC GCCGACCTGT TCAGGCTGGT GCCCTTCCTG GTGTTTGTGG
     3061 TGGTGCCTTT CATGGAATTT CTGCTGCCCG TGGTCGTGAA GCTGTTCCCC AACATGCTGC
     3121 CCAGCACCTT CGAGACACAG AGCATCAAAG AGGAACGGCT GAAGAAAGAG CTGCGGGTCA
     3181 AGCTGGAACT GGCCAAGTTT CTGCAGGACA CCATCGAGGA AATGGCCCTG AAGAACAAGG
     3241 CCGCCAAGGG CAACGCCACC AAGGACTTCT CTGCATTCTT TCAGAAGATC AGAGAGACAG
     3301 GCGAGAGGCC CAGCAACGAG GAAATCATGA GGTTCAGCAA GCTGTTCGAG GATGAGCTGA
     3361 CCCTGGACAA CCTGACCAGG CCTCAACTGG TGGCTCTGTG CAAGCTGCTC GAACTGCAGT
     3421 CTATCGGGAC CAACAACTTC CTGAGATTCC AGCTGACCAT GAGGCTGCGG AGCATCAAGG
     3481 CCGACGACAA GCTGATCTCT GAGGAAGGCG TGGACTCCCT GACCGTCAAA GAACTGCAGG
     3541 CCGCCTGTAG AGCCAGGGGC ATGAGAGCTT TGGGAGTGAC CGAGGACAGA CTGAAGGGCC
     3601 AGCTGAAACA GTGGCTGGAC CTGCACCTGT ACCACGAGAT CCCTACCAGC CTGCTGATCC
     3661 TGTCCAGGGC CATGTACCTG CCAGATACAC TGAGCCCTGC CGACCAGCTG AAGTCTACCC
     3721 TGCAGACACT GCCTGAGATC GTGGCCAAAG AGGCCCAAGT CAAGGCCGCT GAAGTGGAAG
     3781 GCGAACAGGT GGACAACAAG GCCAAACTGG AAGCCACACT GCAAGAGGAA GCCGCCATCC
     3841 AGCAAGAGCA CCTGGAAGAA CTGAAGAGAG CCGCCGAGAC AGCCAAGGAC ATTCAGCCAG
     3901 AAGTGGCCGA GGCTACCGTT CCTGGTAGAC CTGGTGCTGA GCTGCAGCCC AAGATGGTGG
     3961 ATGTGATCCC TCCTAGCGAG ATCCTGAAGG ATACAGCCCC TGTGCTGGAA GGCCTGAAGG
     4021 GCGAAGAGAT CACCAAAGAA GAGATCGACA TCCTGAGCGA CGCCTGCTCC AAGCTGAAAG
     4081 AGCAGAAAAA GAGCCTGACG AAAGAGAAAG AGGAATTGGA GCTGTTGAAA GAGGACGTCC
     4141 AGGACTACAG CGAGGACCTG CAAGAGATCA AGAAAGAACT GAGCAAGACC GGCGACGAGA
     4201 AGTACATCGA AGAGAGCACC GCCTCCAAGA GGCTGTCCAA GAGGGTCCAG CAGATGATCG
     4261 GCCAGATCGA CGGCCTGATC ACCCAGCTGG AAACCACACA GCAGAACGGC AAGCTGGACC
     4321 CAGCTGCTGC TAGTTCTCCT ACTGGCGAGA GCGTGATCAG CGTGGACGAA CTGATCAGCG
     4381 CCATGAAGCA GATCAAGCAC ATCCCCGAGC ACAAACTGAT CTCCCTGACA AGCGCCCTGG
     4441 ATGAGAACAA GGACGGCAAC ATCAACATCG ACGACCTGGT CAAAGTGATC GACCTCGTGA
     4501 ACAAAGAAGA TGTGCAGATC AGCACTACCC AGGTGGCCGA AATCGTGGCT ACCCTGGAAA
     4561 AAGAGGAAAA GGTCGAGGAA AAAGAAAAGG CTAAAGAGAA GGCCGAGAAA GAAGCTGCCG
     4621 AAGTCAAGAA CGGACGACCG GTATAAAGAT CTgaattcga tatcTGAAAT CTCGAGTCTA
     4681 GAGATATCaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     4741 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     4801 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     4861 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     4921 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     4981 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     5041 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     5101 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     5161 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     5221 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     5281 cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta
     5341 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     5401 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     5461 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     5521 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga
     5581 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag
     5641 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
     5701 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
     5761 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
     5821 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     5881 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     5941 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
     6001 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     6061 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     6121 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     6181 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     6241 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     6301 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     6361 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     6421 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     6481 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     6541 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     6601 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     6661 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     6721 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     6781 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     6841 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     6901 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     6961 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     7021 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     7081 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
     7141 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
     7201 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
     7261 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
     7321 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
     7381 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
     7441 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
     7501 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
     7561 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     7621 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     7681 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     7741 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     7801 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     7861 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7921 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7981 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     8041 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     8101 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8161 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8221 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8281 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8341 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8401 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8461 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8521 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8581 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8641 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     8701 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8761 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8821 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8881 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8941 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9001 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9061 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9121 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9181 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9241 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9301 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     9361 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9421 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9481 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9541 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9601 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9661 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9721 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9781 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9841 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     9901 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9961 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
    10021 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
    10081 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
    10141 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
    10201 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
    10261 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
    10321 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
    10381 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
    10441 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
    10501 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
    10561 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
    10621 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
    10681 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
    10741 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
    10801 gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga
    10861 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
    10921 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
    10981 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
    11041 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
    11101 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
    11161 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
    11221 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
    11281 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
    11341 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
    11401 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
    11461 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
    11521 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
    11581 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
    11641 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
    11701 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
    11761 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
    11821 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
    11881 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
    11941 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
    12001 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
    12061 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
    12121 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
    12181 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
    12241 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
    12301 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
    12361 gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct
    12421 gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
    12481 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
    12541 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt
    12601 ggttaattaa cattatggcc ttaggtcact tcatctccat ggggttcttc ttctgatttt
    12661 ctagaaaatg agatgggggt gcagagagct tcctcagtga cctgcccagg gtcacatcag
    12721 aaatgtcaga gctagaactt gaactcagat tactaatctt aaattccatg ccttgggggc
    12781 atgcaagtac gatatacaga aggagtgaac tcattagggc agatgaccaa tgagtttagg
    12841 aaagaagagt ccagggcagg gtacatctac accacccgcc cagccctggg tgagtccagc
    12901 cacgttcacc tcattatagt tgcctctctc cagtcctacc ttgacgggaa gcacaagcag
    12961 aaactgggac aggagcccca ggagaccaaa tcttcatggt ccctctggga ggatgggtgg
    13021 ggagagctgt ggcagaggcc tcaggagggg ccctgctgct cagtggtgac agataggggt
    13081 gagaaagcag acagagtcat tccgtcagca ttctgggtct gtttggtact tcttctcacg
    13141 ctaaggtggc ggtgtgatat gcacaatggc taaaaagcag ggagagctgg aaagaaacaa
    13201 ggacagagac agaggccaag tcaaccagac caattcccag aggaagcaaa gaaaccatta
    13261 cagagactac aagggggaag ggaaggagag atgaattagc ttcccctgta aaccttagaa
    13321 cccagctgtt gccagggcaa cggggcaata cctgtctctt cagaggagat gaagttgcca
    13381 gggtaactac atcctgtctt tctcaaggac catcccagaa tgtggcaccc actagccgtt
    13441 accatagcaa ctgcctcttt gccccactta atcccatccc gtctgttaaa agggccctat
    13501 agttggaggt gggggaggta ggaagagcga tgatcacttg tggactaagt ttgttcacat
    13561 ccccttctcc aaccccctca gtacatcacc ctgggagaac aaggtccact tgcttctggg
    13621 cccacacagt cctgcagtat tgtgtatata aggccagggc aacggaggag caggttttga
    13681 agtgaaaggc aggcaggtgt tggggaggca gttaccgggg caacgggaac agggcgtttc
    13741 ggaggtggtt gccatgggga cctggatgct gacgaaggct cgcgaggctg tgagcagcca
    13801 cagtgccctg ctcagaagcc ccaagctcgt caatcaagct ggttctccat ttgcactcag
    13861 gagcacgggc aggcgagtgg cccctagttc tgggggcagc gggGgatcc
//