LOCUS       pLV-XXX-hCDC6p-Venus-NL 8706 bp ds-DNA     circular     31-MAY-2022
DEFINITION  .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     - From Yilin (p59) Imported using the Genbank importer. File name:
            pLV-XXX-minCMV-Venus-NLS-PEST-IRES-Puro.gbk lentiviral backbone.
            Source name: pLV-EF1a-IRES-Puro (Note: Geneious sometimes stores
            the sequence name in the source attribute) ------------------------
            ---------------------------------------------------------------- -
            Vector: pLV-XXX-hCDC6p-Venus-NLS-PEST-IRES-Puro (EcoRI/XmaI) -
            Insert: #1 PCR product(bGH polyA signal) - Insert:  #2 PCR
            product(PGKpro-PuroRstop) *promoter region can be cut out with
            AgeI/BstBI
FEATURES             Location/Qualifiers
     LTR             1..634
                     /label="3' LTR"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1303..1536
                     /label="RRE"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2028..2143
                     /label="cPPT/CTS"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2161..3516
                     /label="gBlocks"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2191..3486
                     /label="human CDC6 promoter (1296bp/gBlocks)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    3220..3227
                     /label="E2F binding site 1"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    3248..3459
                     /label="5'-truncated 5'UTR (updated)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    3248..3459
                     /label="exon 1 (updated)"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3255..3262
                     /label="E2F binding site 2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    3271..3406
                     /label="5'UTR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    3354..3358
                     /label="cell Cycle gene Homology Region; CHR"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    3384..3388
                     /label="cell Cycle gene Homology Region; CHR"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    3407..3486
                     /label="5'-truncated intron"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    3487..3492
                     /label="BstBI"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     source          3493..4398
                     /label="minCMV-mVenus-NLS-PEST gblock"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             3493..4398
                     /label="Translation 3493-4398"
                     /translation="TSNSSATMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSGGPKKKRKVGSGHGFPPAVAAQDDGTLPMSCAQESGMDRHPAACASARINV*EF"
     regulatory      3511..3516
                     /label="vertebrate consensus sequence for stronginitiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             3517..4395
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    4132..4134
                     /label=" The revertant of the monomeric substitution A206K"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          4167..4188
                     /label="EGFP-C-For"
                     /note="sequence: CATGGTCCTGCTGGAGTTCGTG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             4246..4266
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             4246..4266
                     /label="Translation 4246-4266"
                     /translation="KKKRKVG"
     CDS             4276..4392
                     /label="mODC fragment (EVEE-->AVAA mutation to reducelifetime, same mutation in Clontech pZsGreen1-DR)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          4372..4442
                     /label="PEST-bGHpolyA-Fw"
                     /note="sequence: tctgctaggatcaatgtgtaggaattcTCTAGACCTAGGGGATCCTGATCAcgactgtgccttctagttgc"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     polyA_signal    4423..4650
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    4516..4521
                     /label="polyA signal"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(4631..4682)
                     /label="bGHpolyA-WPRE-Rv"
                     /note="sequence: attgttccagacgcgccccgcccgggctcgagccatagagcccaccgcatcc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    4683..5271
                     /label="WPRE"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     complement(4736..4756)
                     /label="WPRE-R"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(4755..4772)
                     /label="pWPTRev"
                     /note="sequence: CATTAAAGCAGCGTATCC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             complement(5154..5165)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     LTR             5478..6111
                     /label="3' LTR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     complement(6239..6255)
                     /label="M13 Reverse"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(6239..6255)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(6252..6274)
                     /label="M13/pUC Reverse"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     protein_bind    6263..6279
                     /label="lac operator"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     promoter        complement(6287..6317)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     protein_bind    6332..6353
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     complement(6470..6487)
                     /label="L4440"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     rep_origin      complement(6641..7229)
                     /label="ori"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     complement(6721..6740)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             complement(7400..8260)
                     /label="AmpR"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     8023..8042
                     /label="Amp-R"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     promoter        complement(8261..8365)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     polyA_signal    8408..8542
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     complement(8445..8464)
                     /label="SV40pA-R"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     8499..8518
                     /label="EBV-rev"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataccggt CAATTCACTG ATGATCGTAA TATTGTTGCT
     2221 TCCCTCTTTC TAGGCTTTAT GGCTCTATTT TGTTTGTTAC TGAGGGGTAA AAGATAAATG
     2281 TTTACCATCA CCTAGAATTG GGTTCTGGCC TCTAAAGGAA CCTGAGGCTT AGATGAATTA
     2341 TTGGCTTTGG AAGCTGGCCT TCAAATTACT GCGCTAATTT ATATTTTTCA TTAAAAACTC
     2401 AGCTTGCCTC TTCTATATAG CTGTCTTCCC TGGCCCTGAA ACCCTAGTGT TTCGCCATAA
     2461 AAGATTTTAA AATTAAGGGG TCATAATTCC CTCCCCATGA TGTGTGGATT AATGGTAAGA
     2521 AGATGCACAG AACATAATAT TCTTAGGTTG AACGAAATAA AAGTAAAGAG TTGGCTCTGT
     2581 TTCTCACCTT TGAAGCACAA ATCAAGAGAT ACTATGATGA AGCATAGTTT TTCTTTATAT
     2641 AGGTGTGTAG AACTTTACCA TAAAAATCAC TAGTTCAGCC ATCAGGAGAT CTGGATCCTA
     2701 GGCTCTTCAC TGTCACCAAG ATGCTGTGAC CTCTAACCTT GTATAGAAGT TTGCTTTGTA
     2761 CTTTGCGAGG TTGAGCATTA GAGAGGTAAG GAAAGTGCCT AGCATCATAC CTGGCGCACA
     2821 GAACCCAAAA CGGTAGGTAT CATGTAGCAG TTCTGAAAAT CTAGCCCATC AGGATGATGC
     2881 AAATGGGTAC TTTAGGCAGT GAGAAGGGGA ACCACATCTT GACACTTCCA GTCGAAGGAA
     2941 GAGTGCGACT GCGCGGCAGC AAAGACTACG CCTCCCAGCG TGCTTTGCGG CGGGCCGGCC
     3001 CGCTTTACCC AGAGTCGCCC TGCCGCAATC GCGCGTCTTT CCACCGAGGC CCCGGATGTA
     3061 GATTCCCTCC CCCGTTCAGT GGTCGTGGCC TCACAGCGAC TCTAAGACTT GGGGCTCTCT
     3121 CATTGGCTGT AACTCTTCCA CTGGATTGGT AGCAAAAAAA GAGGCGGTGC CCAAGGCGAA
     3181 AGGCTCTGTG ACTACAGCCA ATCAGAATCG AGGCCGGGCT TTGGCGGGAG GTGGGAACGC
     3241 TGTGGCCATT CGGATTTGGC GCGAGCGCGG CTGGAGTTTG CTGCTGCCGC TGTGCAGTTT
     3301 GTTCAGGGGC TTGTGGTGGT GAGTCCGAGA GGCTGCGTGT GAGAGACGTG AGAAGGATCC
     3361 TGCACTGAGG AGGTGGAAAG AAGAGGATTG CTCGAGGAGG CCTGGGGTCT GTGAGGCAGC
     3421 GGAGCTGGGT GAAGGCTGCG GGTTCCGGCG AGGCCTGAGG TGAAGTGAAG GGAAAAGAGC
     3481 TGAGCTttcg aaactagtaa ttcgagcgcc accatggtga gcaagggcga ggagctgttc
     3541 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     3601 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gctgatctgc
     3661 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctggg ctacggcctg
     3721 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     3781 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     3841 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     3901 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     3961 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc
     4021 cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc
     4081 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc
     4141 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     4201 atcactctcg gcatggacga gctgtacaag ggaAGTGGtg gaccaaagaa gaagagaaag
     4261 gtaggtAGTG Gacatggctt cccgccggca gtggcagcac aggatgatgg cacgctgccc
     4321 atgtcttgtg cccaggagag cgggatggac cgtcaccctg cagcctgtgc ttctgctagg
     4381 atcaatgtgt aggaattcTC TAGACCTAGG GGATCCTGAT CAcgactgtg ccttctagtt
     4441 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     4501 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     4561 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     4621 ggcatgctgg ggatgcggtg ggctctatgg ctcgagcccg ggcggggcgc gtctggaaca
     4681 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     4741 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     4801 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     4861 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     4921 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     4981 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     5041 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     5101 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     5161 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     5221 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattaatt
     5281 ctgcagtcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     5341 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     5401 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     5461 agaaaagagg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
     5521 gtggatctac cacacacaag gctacttccc tgattagcag aactacacac cagggccagg
     5581 ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa
     5641 ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg
     5701 gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca
     5761 tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta
     5821 caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg
     5881 gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt
     5941 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     6001 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     6061 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     6121 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc
     6181 cttgacattg ctagcgttta ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     6241 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     6301 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     6361 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     6421 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     6481 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6541 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6601 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6661 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6721 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6781 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6841 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6901 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6961 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7021 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     7081 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7141 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     7201 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7261 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     7321 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     7381 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     7441 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     7501 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     7561 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     7621 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     7681 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     7741 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     7801 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7861 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7921 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7981 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     8041 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     8101 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     8161 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     8221 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     8281 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     8341 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac Sggatcggga
     8401 gatcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     8461 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     8521 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
     8581 caccccatac cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga
     8641 ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt
     8701 agtagt
//