LOCUS       Exported                4550 bp ds-DNA     circular SYN 16-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Nov 17, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      22..858
                     /label=more stringent p15A
                     /label=more\stringent\p15A
     CDS             complement(930..1745)
                     /label=Kanamycin phosphotransferase
                     /label=Kanamycin\phosphotransferase
     terminator      1846..1891
                     /label=L3S3P21
                     /note="Geneious type: terminator"
     misc_feature    1898..1901
                     /label=4bp_scar5
     promoter        1922..2011
                     /label=pCymRC promoter
                     /label=pCymRC
     misc_feature    1922..1953
                     /label=CymO
                     /note="Geneious type: operator"
     promoter        1945..1950
                     /label=-35 promoter
                     /label=-35
     promoter        1968..1973
                     /label=-10 promoter
                     /label=-10
     misc_feature    1980..2011
                     /label=CymO
                     /note="Geneious type: operator"
     misc_feature    2013..2086
                     /label=RiboJ53
                     /note="Geneious type: riboj"
     RBS             2087..2111
                     /label=RBS
                     /note="strength = 47274"
     CDS             2112..3458
                     /codon_start=1
                     /product="CYP002"
                     /label=CYP002
                     /db_xref="UniProtKB/Swiss-Prot:P55540"
                     /db_xref="Refseq:GI:16519831|GI:2182499|GI:499177759|GI:249
                     3410|EMBL-CDS:AAB 
                     91753.1|REFSEQ:NP_443951.1|REFSEQ:WP_010875299.1"
                     /translation="MNVLLNPLNRRHRLRYDIPVMPGAFPLVGHLPAIVCDLPRLLRRA
                     ERTLGSHFWLDFGPAGHLMTCVDPHAFALLRHKDVSSALIEEIAPELLGGTLVAQDGGA
                     HRQARDAIKAAFLPEGLTQAGIGDLFAPVIRARVQAWRDRGDVTILPETGDLMLKLIFT
                     LMGVPAQDLPGWHRKYRQLLQLIVAPSVDLPGLPLRRGRAARDWIDAQLRQFVRDARAH
                     AARTGLINDMVSAFDRSDDALSDDLLVANIRLLLLAGHDTTASTMAWMVIELARQPMLW
                     DALVEEAQRVGAVPTRHADLEQCPVAEALFRETLRVHPATTLLPRRALQELQLGQRRIP
                     AGTHLCIPLLHFSTSALLHEAPDQFRLARWLQRTEPIRPVDMLQFGTGPHVCIGYHLVW
                     LELVQFSIALALTMHKAGVRPLLLSGVEKGRRYYPTAHPSMTIRIGFS*"
     primer_bind     2570..2589
                     /label=seq1_CYP02
     primer_bind     3283..3300
                     /label=seq2_CYP02
     terminator      3502..3548
                     /label=L3S3P41
     misc_feature    3544
                     /label=C to G
     misc_feature    3558..3561
                     /label=4bp_scar6
     promoter        3577..3658
                     /label=Constitutive Promoter + RBS (DNA2.0 Vector)
                     /note="Geneious type: promoter"
     promoter        3595..3600
                     /label=-35 promoter
                     /label=-35
     misc_feature    3596
                     /label=C
                     /note="Geneious type: Editing History Replacement"
     promoter        3618..3623
                     /label=-10 promoter
                     /label=-10
     CDS             3659..4270
                     /label=CymR
     misc_feature    3956..3958
                     /label=E100D
                     /note="Geneious type: mutation"
     misc_feature    3958
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3986..3988
                     /label=S110
                     /note="Geneious type: mutation"
     misc_feature    3986
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4040..4042
                     /label=Q128L
                     /note="Geneious type: mutation"
     misc_feature    4041
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4169..4171
                     /label=A171
                     /note="Geneious type: mutation"
     misc_feature    4169
                     /label=G
                     /note="Geneious type: Editing History Replacement"
     terminator      4280..4534
                     /label=induction operon terminator region
ORIGIN
        1 actgactcgc tgcacgaggc agacctcagc gctagcggag tgtatactgg cttactatgt
       61 tggcactgat gagggtgtca gtgaagtgct tcatgtggca ggagaaaaaa ggctgcaccg
      121 gtgcgtcagc agaatatgtg atacaggata tattccgctt cctcgctcac tgactcgcta
      181 cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg
      241 gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc
      301 ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa
      361 acccgacagg actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc
      421 ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt
      481 ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa
      541 ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      601 gaaagacatg caaaagcacc actggcagca gccactggta attgatttag aggagttagt
      661 cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca agttttggtg actgcgctcc
      721 tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc
      781 ctgcaaggcg gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca
      841 agaagatcat cttattaagg ggtctgacgc tcagtggaac gaaaaatcaa tctaaagtat
      901 atatgagtaa acttggtctg acagttacct tagaaaaact catcgagcat caaatgaaac
      961 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     1021 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     1081 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     1141 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc
     1201 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     1261 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
     1321 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     1381 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg
     1441 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     1501 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     1561 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
     1621 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     1681 tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg ttgaatatgg
     1741 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat
     1801 atatttttat cttgtgcaat gtacatcaga gattttgaga cacaaccaat tattgaaggc
     1861 ctccctaacg gggggccttt ttttgtttct ggtctcccaa gcgcttaacg atcgttggct
     1921 gaacaaacag acaatctggt ctgtttgtat tatggaaaat ttttctgtat aatagattca
     1981 acaaacagac aatctggtct gtttgtatta tcagcggtca acgcatgtgc tttgcgttct
     2041 gatgagacag tgatgtcgaa accgcctcta caaataattt tgtttaggcc aagaacaccc
     2101 gggaggttat aatgaatgtc ctgctgaatc cgttgaatcg ccgccatcgt ctgcgatatg
     2161 acattccagt aatgcctggt gcgttccccc ttgtcgggca tttgccggcg attgtatgtg
     2221 atctgccacg cttactccgc cgggcagaac gtactctcgg tagtcatttt tggctggatt
     2281 tcggtccggc aggtcatttg atgacctgtg ttgatccgca cgcgttcgcg ctgttgcgcc
     2341 acaaagatgt tagtagcgcg ttgattgagg aaatcgctcc cgaactgctc ggcggcacct
     2401 tggtagcaca agatggcggt gcccatcgcc aggcgcgtga tgcaatcaaa gcagccttcc
     2461 tgccagaagg tttaacccag gcggggattg gcgatctttt tgctccggtg attcgtgcgc
     2521 gtgtgcaagc gtggcgtgat cgtggcgacg taaccatact gcctgaaaca ggcgacctga
     2581 tgcttaaact gatttttacc ctgatgggcg ttcctgcaca ggacctgccg ggttggcacc
     2641 gtaaatatcg tcaacttctg caacttattg tcgcccctag tgtggatctg ccaggattgc
     2701 cacttcgccg tggccgcgca gcacgtgatt ggattgacgc gcaactgcgc caattcgttc
     2761 gcgacgcgcg tgcacatgcc gcccggacag gtctgattaa tgatatggta agcgcgttcg
     2821 atcgctccga tgatgccctg tctgacgatc tgttggtggc taatattcgt ctgctgttgc
     2881 ttgcaggaca tgataccacg gcgagcacaa tggcatggat ggtgattgaa ctggccagac
     2941 aaccaatgtt gtgggatgcg ctggttgaag aagcccagcg tgtgggagcg gttccgaccc
     3001 gtcatgccga tttagaacaa tgtcctgtgg cggaagccct gttccgcgaa acactgcgcg
     3061 ttcacccggc gacgacttta ctgccacgcc gcgcattaca agaactccag ttgggccaac
     3121 gcagaattcc tgcgggcaca catctgtgca taccgttact gcattttagc acctctgctc
     3181 tgttacatga agccccggac cagtttcgcc tggcacgctg gttgcagcga accgagccga
     3241 tacggccggt tgatatgctg cagtttggaa ccggcccgca tgtgtgtatt ggttaccacc
     3301 tggtctggct tgagcttgtc cagttcagta tagccctggc gctgacaatg cataaagctg
     3361 gagtgcgccc gctcctgctt agcggcgttg aaaaaggtcg cagatactac ccgacggcgc
     3421 atccgagcat gacgattcgt atcggatttt cgtaataagg atgagctcta caaataagca
     3481 gaggtggttg tgttgcgaaa aaaaaaaaaa aaacacccta acgggtgttt tttttttttt
     3541 ggtgtcccca cgtgtggcgc tggagaccgt ccaatggcgg cgcgccatcg aatggtgcaa
     3601 aacctttcgc ggtatggcat gatagcgccc ggaagagagt caattcaggg tggtgaatat
     3661 gagcccgaaa cgtcgtaccc aggcagaacg tgcaatggaa acccagggta aactgattgc
     3721 agcagcactg ggtgttctgc gtgaaaaagg ttatgcaggt tttcgtattg cagatgttcc
     3781 gggtgcagcc ggtgttagcc gtggtgcaca gagccatcat tttccgacca aactggaact
     3841 gctgctggca acctttgaat ggctgtatga gcagattacc gaacgtagcc gtgcacgtct
     3901 ggcaaaactg aaaccggaag atgatgttat tcagcagatg ctggatgatg cagcagattt
     3961 ttttctggat gatgatttta gcatcggcct ggatctgatt gttgcagcag atcgtgatcc
     4021 ggcactgcgt gaaggtattc tgcgtaccgt tgaacgtaat cgttttgttg ttgaagatat
     4081 gtggctgggt gtgctggtga gccgtggtct gagccgtgat gatgccgaag atattctgtg
     4141 gctgattttt aacagcgttc gtggtctgac agttcgtagc ctgtggcaga aagataaaga
     4201 acgttttgaa cgtgtgcgta atagcaccct ggaaattgca cgtgaacgtt atgcaaaatt
     4261 caaacgttga taaggatcct aattggtaac gaatcagaca attgacggct cgagggagta
     4321 gcatagggtt tgcagaatcc ctgcttcgtc catttgacag gcacattatg catcgatgat
     4381 aagctgtcaa acatgagcag atcctctacg ccggacgcat cgtggccggc atcaccggcg
     4441 ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc
     4501 gccacttcgg gctcatgagc aaatatttta tctgaggtgc ttcctcgctc
//
