LOCUS       Exported                4580 bp ds-DNA     circular SYN 16-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Nov 17, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      22..858
                     /label=more stringent p15A
                     /label=more\stringent\p15A
     CDS             complement(930..1745)
                     /label=Kanamycin phosphotransferase
                     /label=Kanamycin\phosphotransferase
     terminator      1846..1891
                     /label=L3S3P21
                     /note="Geneious type: terminator"
     misc_feature    1898..1901
                     /label=4bp_scar5
     promoter        1922..2011
                     /label=pCymRC promoter
                     /label=pCymRC
     misc_feature    1922..1953
                     /label=CymO
                     /note="Geneious type: operator"
     promoter        1945..1950
                     /label=-35 promoter
                     /label=-35
     promoter        1968..1973
                     /label=-10 promoter
                     /label=-10
     misc_feature    1980..2011
                     /label=CymO
                     /note="Geneious type: operator"
     misc_feature    2013..2086
                     /label=RiboJ53
                     /note="Geneious type: riboj"
     RBS             2087..2112
                     /label=RBS
                     /note="strength = 49714"
     CDS             2113..3489
                     /codon_start=1
                     /product="CYP005"
                     /label=CYP005
                     /db_xref="UniProtKB/Swiss-Prot:A0A0H3KUT7"
                     /db_xref="Refseq:GI:501174601|GI:189338338|GI:160346524|EMB
                     L-CDS:BAG47406.1| 
                     REFSEQ:WP_012218293.1|GI:685684764|GI:1061780332|GI:9797243
                      
                     35|EMBL-CDS:PRE95249.1|EMBL-CDS:PRF53850.1|EMBL-CDS:PRF5850
                      8.1"
                     /translation="MLLNPLSRRVRMQQGMPVLPGAFPVVGHLPAVARALPDLLAYGER
                     RWGTHFWLDMGAMGRMLVCTRPEAFELFNNKDVLADLLGDVAPAMRDTMATQDGELHRR
                     TRAWANPSFQPQGLSAAKVGELFADIMMAWIRAWPARGTINLVDETSALTLTMIFRMLG
                     IAEDDIATWETHYRHYALVSLLPVDLPGTPLRRSRRAQVWIDARLSALVNELGRSPGDS
                     GLLATLIRSPAAPDASSPESNLLGNLRALIHAAHKTTAVTMAHLAIRLAEQPDHWDALV
                     AEARAAGTVPRNPSELARFPFAEALFLETLRSEPAFPLVFRRTRERIELGGRELAAGTR
                     LIIPLILLSRAPSLYPQPDTFLPQRWLEHRGAHKPIQTLQFGSGPHRCLGFHTACMQLV
                     QFAVTLALVLDERKMRPQLVPQRTCGRRRFPINYPPRDTQVAFRSVSSNHDNRSEIPS*
                     "
     primer_bind     2581..2600
                     /label=seq1_CYP05
     primer_bind     3284..3301
                     /label=seq2_CYP05
     terminator      3532..3578
                     /label=L3S3P41
     misc_feature    3574
                     /label=C to G
     misc_feature    3588..3591
                     /label=4bp_scar6
     promoter        3607..3688
                     /label=Constitutive Promoter + RBS (DNA2.0 Vector)
                     /note="Geneious type: promoter"
     promoter        3625..3630
                     /label=-35 promoter
                     /label=-35
     misc_feature    3626
                     /label=C
                     /note="Geneious type: Editing History Replacement"
     promoter        3648..3653
                     /label=-10 promoter
                     /label=-10
     CDS             3689..4300
                     /label=CymR
     misc_feature    3986..3988
                     /label=E100D
                     /note="Geneious type: mutation"
     misc_feature    3988
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4016..4018
                     /label=S110
                     /note="Geneious type: mutation"
     misc_feature    4016
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4070..4072
                     /label=Q128L
                     /note="Geneious type: mutation"
     misc_feature    4071
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4199..4201
                     /label=A171
                     /note="Geneious type: mutation"
     misc_feature    4199
                     /label=G
                     /note="Geneious type: Editing History Replacement"
     terminator      4310..4564
                     /label=induction operon terminator region
ORIGIN
        1 actgactcgc tgcacgaggc agacctcagc gctagcggag tgtatactgg cttactatgt
       61 tggcactgat gagggtgtca gtgaagtgct tcatgtggca ggagaaaaaa ggctgcaccg
      121 gtgcgtcagc agaatatgtg atacaggata tattccgctt cctcgctcac tgactcgcta
      181 cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg
      241 gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc
      301 ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa
      361 acccgacagg actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc
      421 ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt
      481 ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa
      541 ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      601 gaaagacatg caaaagcacc actggcagca gccactggta attgatttag aggagttagt
      661 cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca agttttggtg actgcgctcc
      721 tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc
      781 ctgcaaggcg gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca
      841 agaagatcat cttattaagg ggtctgacgc tcagtggaac gaaaaatcaa tctaaagtat
      901 atatgagtaa acttggtctg acagttacct tagaaaaact catcgagcat caaatgaaac
      961 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     1021 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     1081 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     1141 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc
     1201 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     1261 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
     1321 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     1381 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg
     1441 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     1501 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     1561 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
     1621 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     1681 tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg ttgaatatgg
     1741 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat
     1801 atatttttat cttgtgcaat gtacatcaga gattttgaga cacaaccaat tattgaaggc
     1861 ctccctaacg gggggccttt ttttgtttct ggtctcccaa gcgcttaacg atcgttggct
     1921 gaacaaacag acaatctggt ctgtttgtat tatggaaaat ttttctgtat aatagattca
     1981 acaaacagac aatctggtct gtttgtatta tcagcggtca acgcatgtgc tttgcgttct
     2041 gatgagacag tgatgtcgaa accgcctcta caaataattt tgtttagagc cagcactata
     2101 aggaggttat tgatgctgct gaacccgtta agtcgtcgtg tgcgcatgca gcaaggcatg
     2161 cccgtgttac cgggcgcgtt cccggtcgta ggccatctgc ccgctgttgc gcgcgcactg
     2221 cctgatctgc tggcatacgg cgaacgtaga tggggcaccc atttctggtt ggacatgggc
     2281 gcgatgggtc gtatgctggt gtgcacccgc cccgaggcct ttgaactgtt caacaacaaa
     2341 gacgtgctcg ccgatttact gggagatgtc gctccggcca tgcgtgacac gatggccacg
     2401 caagatggtg aactgcatcg tcgtacccgc gcttgggcga acccatcttt tcaaccacag
     2461 ggtcttagtg ccgccaaggt tggcgaactg ttcgcggata taatgatggc gtggattcgt
     2521 gcttggccag cgcgcggtac aattaacttg gtggacgaaa cgagcgcttt gacgctgaca
     2581 atgatcttcc gtatgttagg gatcgcagag gatgacattg caacgtggga aacccattat
     2641 cgccattatg cgctggtgag cctgcttccc gtggacctgc cgggtacgcc tttacgccgc
     2701 agccgtcgcg cacaagtctg gattgatgcg cgtctgtcag cattggtaaa cgaattgggt
     2761 cgtagcccag gcgactcggg cctcttagcg accctgatcc gttcacccgc ggccccggat
     2821 gcgtcgtcac cagaatcaaa tcttttaggt aacctgcgtg cactcattca cgccgcgcac
     2881 aaaaccaccg cggtaacgat ggctcacttg gcgatccgcc tcgcagagca gccagaccac
     2941 tgggatgcat tagtcgcaga agctcgggct gctggaacgg tgccgcgcaa cccctcggaa
     3001 ctggcacgtt tcccgttcgc tgaggccctc tttctggaaa cgctgcgctc tgagccggca
     3061 tttcccttag tgtttcgtcg cacccgcgaa cgaattgagc tgggcggtcg cgagctggct
     3121 gcaggtacac ggctgattat ccctctgatc ttgttatcgc gtgcgccaag cctgtacccg
     3181 cagccagata ctttcttacc gcagcgctgg ttagagcacc gaggcgccca taaaccaatt
     3241 cagactttac agttcggaag cggtccacat cgttgtctgg gttttcatac tgcctgcatg
     3301 caactggtgc agtttgcagt taccttggcc ctggtgctgg atgaacggaa aatgcgcccg
     3361 caacttgtgc cgcagcggac ttgtgggcgc cgtcgttttc cgattaacta tccaccacgt
     3421 gacacccagg tggcctttag aagtgtttct tcgaatcacg acaaccgtag tgagattccg
     3481 agctaataag gatgagctct acaaataagc agaggtggtt gtgttgcgaa aaaaaaaaaa
     3541 aaacacccta acgggtgttt tttttttttt ggtgtcccca cgtgtggcgc tggagaccgt
     3601 ccaatggcgg cgcgccatcg aatggtgcaa aacctttcgc ggtatggcat gatagcgccc
     3661 ggaagagagt caattcaggg tggtgaatat gagcccgaaa cgtcgtaccc aggcagaacg
     3721 tgcaatggaa acccagggta aactgattgc agcagcactg ggtgttctgc gtgaaaaagg
     3781 ttatgcaggt tttcgtattg cagatgttcc gggtgcagcc ggtgttagcc gtggtgcaca
     3841 gagccatcat tttccgacca aactggaact gctgctggca acctttgaat ggctgtatga
     3901 gcagattacc gaacgtagcc gtgcacgtct ggcaaaactg aaaccggaag atgatgttat
     3961 tcagcagatg ctggatgatg cagcagattt ttttctggat gatgatttta gcatcggcct
     4021 ggatctgatt gttgcagcag atcgtgatcc ggcactgcgt gaaggtattc tgcgtaccgt
     4081 tgaacgtaat cgttttgttg ttgaagatat gtggctgggt gtgctggtga gccgtggtct
     4141 gagccgtgat gatgccgaag atattctgtg gctgattttt aacagcgttc gtggtctgac
     4201 agttcgtagc ctgtggcaga aagataaaga acgttttgaa cgtgtgcgta atagcaccct
     4261 ggaaattgca cgtgaacgtt atgcaaaatt caaacgttga taaggatcct aattggtaac
     4321 gaatcagaca attgacggct cgagggagta gcatagggtt tgcagaatcc ctgcttcgtc
     4381 catttgacag gcacattatg catcgatgat aagctgtcaa acatgagcag atcctctacg
     4441 ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg
     4501 ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc aaatatttta
     4561 tctgaggtgc ttcctcgctc
//
