LOCUS       Exported                4808 bp ds-DNA     circular SYN 16-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Nov 17, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      22..858
                     /label=more stringent p15A
                     /label=more\stringent\p15A
     CDS             complement(930..1745)
                     /label=Kanamycin phosphotransferase
                     /label=Kanamycin\phosphotransferase
     terminator      1846..1891
                     /label=L3S3P21
                     /note="Geneious type: terminator"
     misc_feature    1898..1901
                     /label=4bp_scar5
     promoter        1922..2011
                     /label=pCymRC promoter
                     /label=pCymRC
     misc_feature    1922..1953
                     /label=CymO
                     /note="Geneious type: operator"
     promoter        1945..1950
                     /label=-35 promoter
                     /label=-35
     promoter        1968..1973
                     /label=-10 promoter
                     /label=-10
     misc_feature    1980..2011
                     /label=CymO
                     /note="Geneious type: operator"
     misc_feature    2013..2086
                     /label=RiboJ53
                     /note="Geneious type: riboj"
     RBS             2087..2112
                     /label=RBS
                     /note="strength = 49417"
     CDS             2113..3717
                     /codon_start=1
                     /product="CYP007"
                     /label=CYP007
                     /db_xref="UniProtKB/Swiss-Prot:A0A1I4YL09"
                     /db_xref="Refseq:EMBL-CDS:SFN38758.1"
                     /translation="MEVQETTAACRDAFAELASPARIHDPYPFIRWLREHDPVHRAASG
                     LFLLSRHADIYWALKATGDAFRGPAPGELARYFPHAATSLSLNLLASTLAMKDPPTHTR
                     LRRLISRDFTMRKIDNLRPSIARIVEARLDGMAPALERGEAVDLHQDFALALPILVFAE
                     LFGMPHDDMFGLATGIGAILEGLSPHASDPRLAAADAASARVKAYFGGLIQRKRTHPCH
                     DIVSMLVGAHEDDVDTLSDAELISMLWGMLLGGFTTTAATIDHAVLAMLAYPEQRHWLQ
                     GDAMGVKAFVEEVLRCDAPAMFSSIPRIAQRDIELGGVVIPKNADVRVLIASGNRDPDA
                     FADPDRFDPARFYGTSPGMSTDGKIMLSFGHGIHFCLGAQLARVQLAESLPRIQARFPT
                     LALAGLPTREPSAFLRTFRALPVRLRAQGAEMRVVVDQNLCGTSGQCVLALPGTFRQRE
                     PDGVAEVCRATVPQALHAAVRLAASQCPVAAIRVIRGEAGGDERASADNAPSPAAEAER
                     HADRDQRNPGGHHGTV*"
     primer_bind     2582..2599
                     /label=seq1_CYP07
     primer_bind     3275..3292
                     /label=seq2_CYP07
     terminator      3760..3806
                     /label=L3S3P41
     misc_feature    3802
                     /label=C to G
     misc_feature    3816..3819
                     /label=4bp_scar6
     promoter        3835..3916
                     /label=Constitutive Promoter + RBS (DNA2.0 Vector)
                     /note="Geneious type: promoter"
     promoter        3853..3858
                     /label=-35 promoter
                     /label=-35
     misc_feature    3854
                     /label=C
                     /note="Geneious type: Editing History Replacement"
     promoter        3876..3881
                     /label=-10 promoter
                     /label=-10
     CDS             3917..4528
                     /label=CymR
     misc_feature    4214..4216
                     /label=E100D
                     /note="Geneious type: mutation"
     misc_feature    4216
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4244..4246
                     /label=S110
                     /note="Geneious type: mutation"
     misc_feature    4244
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4298..4300
                     /label=Q128L
                     /note="Geneious type: mutation"
     misc_feature    4299
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4427..4429
                     /label=A171
                     /note="Geneious type: mutation"
     misc_feature    4427
                     /label=G
                     /note="Geneious type: Editing History Replacement"
     terminator      4538..4792
                     /label=induction operon terminator region
ORIGIN
        1 actgactcgc tgcacgaggc agacctcagc gctagcggag tgtatactgg cttactatgt
       61 tggcactgat gagggtgtca gtgaagtgct tcatgtggca ggagaaaaaa ggctgcaccg
      121 gtgcgtcagc agaatatgtg atacaggata tattccgctt cctcgctcac tgactcgcta
      181 cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg
      241 gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc
      301 ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa
      361 acccgacagg actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc
      421 ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt
      481 ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa
      541 ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      601 gaaagacatg caaaagcacc actggcagca gccactggta attgatttag aggagttagt
      661 cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca agttttggtg actgcgctcc
      721 tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc
      781 ctgcaaggcg gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca
      841 agaagatcat cttattaagg ggtctgacgc tcagtggaac gaaaaatcaa tctaaagtat
      901 atatgagtaa acttggtctg acagttacct tagaaaaact catcgagcat caaatgaaac
      961 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     1021 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     1081 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     1141 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc
     1201 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     1261 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
     1321 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     1381 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg
     1441 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     1501 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     1561 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
     1621 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     1681 tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg ttgaatatgg
     1741 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat
     1801 atatttttat cttgtgcaat gtacatcaga gattttgaga cacaaccaat tattgaaggc
     1861 ctccctaacg gggggccttt ttttgtttct ggtctcccaa gcgcttaacg atcgttggct
     1921 gaacaaacag acaatctggt ctgtttgtat tatggaaaat ttttctgtat aatagattca
     1981 acaaacagac aatctggtct gtttgtatta tcagcggtca acgcatgtgc tttgcgttct
     2041 gatgagacag tgatgtcgaa accgcctcta caaataattt tgtttaggta tatttacaag
     2101 gaggtaacta atatggaggt gcaggaaact actgccgcgt gccgcgatgc atttgctgaa
     2161 ttagcgagtc cggcacgcat acatgatcct tatccgttca ttcgttggct ccgggaacat
     2221 gatcccgtgc atcgcgccgc atcaggactg tttttactga gccgtcatgc cgatatttat
     2281 tgggcactca aggcgactgg cgatgcgttt cgcggcccag cgccgggaga attagcacgt
     2341 tatttccctc atgcggcgac atccttatcg ctgaatttgc ttgcgagtac cttggcgatg
     2401 aaagatccgc caactcatac ccgcttgcgt cgtctgataa gtcgtgactt tacgatgcgc
     2461 aaaatagaca acttgcggcc tagcattgcg cgtattgtgg aagcacgcct ggacggcatg
     2521 gcaccagcct tagaaagagg cgaagcagtg gatctccatc aggattttgc cctcgcgctg
     2581 ccgatcctgg tttttgcaga actttttggg atgccgcacg atgatatgtt cggcttggct
     2641 accggcattg gagccatcct ggaaggatta agccctcatg catctgatcc cagactggcg
     2701 gcggcagatg ccgcctctgc gcgtgtaaaa gcctattttg gaggcctgat acagcgtaaa
     2761 cgcactcatc cttgccatga catagtaagt atgctggtag gtgcgcacga agatgatgtg
     2821 gacaccctta gcgacgccga actgattagt atgctgtggg gcatgttact gggtggcttt
     2881 acaaccactg cggctacaat agatcatgct gtactcgcaa tgctggccta cccggaacaa
     2941 cgccactggc tgcaggggga tgcgatgggc gtaaaagcat ttgttgagga agtgttaaga
     3001 tgcgatgccc ccgccatgtt ttcgtcaatt ccacgcatcg cgcagcgtga catcgaattg
     3061 ggtggagtcg ttattccgaa aaacgctgac gtccgggtgc tgatcgcttc tggtaatcgt
     3121 gatccagatg ccttcgcgga ccctgatcgg tttgatccgg cacgctttta cggcaccagc
     3181 ccaggtatgt caactgatgg caagattatg ctgtcctttg gtcacggcat ccacttctgc
     3241 ctgggggcac aattagcacg cgttcagctg gccgaaagcc tgccacgcat tcaggcgcga
     3301 tttcccacac tggccctggc cggtttgcca acccgagagc cctcagcgtt tctgcgtacc
     3361 tttcgtgcgt taccggtacg cctgcgtgca caaggtgcgg aaatgcgagt cgttgtagat
     3421 cagaaccttt gtggtacttc aggccagtgt gtgctggctt tacctggtac gttccgccaa
     3481 cgagaaccgg atggcgtggc ggaagtatgt cgagcaacgg taccgcaggc gctgcatgct
     3541 gccgtgcgtc tggctgcatc tcagtgcccg gttgcggcca tccgcgttat acgtggtgaa
     3601 gcaggcggtg atgagcgcgc atctgcggac aacgctccga gccctgcagc cgaagctgaa
     3661 cgtcacgccg atcgtgatca gcgtaacccg ggtgggcacc acggtaccgt ctaataagga
     3721 tgagctctac aaataagcag aggtggttgt gttgcgaaaa aaaaaaaaaa acaccctaac
     3781 gggtgttttt ttttttttgg tgtccccacg tgtggcgctg gagaccgtcc aatggcggcg
     3841 cgccatcgaa tggtgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca
     3901 attcagggtg gtgaatatga gcccgaaacg tcgtacccag gcagaacgtg caatggaaac
     3961 ccagggtaaa ctgattgcag cagcactggg tgttctgcgt gaaaaaggtt atgcaggttt
     4021 tcgtattgca gatgttccgg gtgcagccgg tgttagccgt ggtgcacaga gccatcattt
     4081 tccgaccaaa ctggaactgc tgctggcaac ctttgaatgg ctgtatgagc agattaccga
     4141 acgtagccgt gcacgtctgg caaaactgaa accggaagat gatgttattc agcagatgct
     4201 ggatgatgca gcagattttt ttctggatga tgattttagc atcggcctgg atctgattgt
     4261 tgcagcagat cgtgatccgg cactgcgtga aggtattctg cgtaccgttg aacgtaatcg
     4321 ttttgttgtt gaagatatgt ggctgggtgt gctggtgagc cgtggtctga gccgtgatga
     4381 tgccgaagat attctgtggc tgatttttaa cagcgttcgt ggtctgacag ttcgtagcct
     4441 gtggcagaaa gataaagaac gttttgaacg tgtgcgtaat agcaccctgg aaattgcacg
     4501 tgaacgttat gcaaaattca aacgttgata aggatcctaa ttggtaacga atcagacaat
     4561 tgacggctcg agggagtagc atagggtttg cagaatccct gcttcgtcca tttgacaggc
     4621 acattatgca tcgatgataa gctgtcaaac atgagcagat cctctacgcc ggacgcatcg
     4681 tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg
     4741 atggggaaga tcgggctcgc cacttcgggc tcatgagcaa atattttatc tgaggtgctt
     4801 cctcgctc
//
