LOCUS       Exported                4498 bp ds-DNA     circular SYN 16-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Nov 17, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      22..858
                     /label=more stringent p15A
                     /label=more\stringent\p15A
     CDS             complement(930..1745)
                     /label=Kanamycin phosphotransferase
                     /label=Kanamycin\phosphotransferase
     terminator      1846..1891
                     /label=L3S3P21
                     /note="Geneious type: terminator"
     misc_feature    1898..1901
                     /label=4bp_scar5
     promoter        1922..2011
                     /label=pCymRC promoter
                     /label=pCymRC
     misc_feature    1922..1953
                     /label=CymO
                     /note="Geneious type: operator"
     promoter        1945..1950
                     /label=-35 promoter
                     /label=-35
     promoter        1968..1973
                     /label=-10 promoter
                     /label=-10
     misc_feature    1980..2011
                     /label=CymO
                     /note="Geneious type: operator"
     misc_feature    2013..2086
                     /label=RiboJ53
                     /note="Geneious type: riboj"
     RBS             2087..2111
                     /label=RBS
                     /note="strength = 48780"
     CDS             2112..3407
                     /codon_start=1
                     /product="CYP010"
                     /label=CYP010
                     /db_xref="UniProtKB/Swiss-Prot:A0A2R4EAI7"
                     /db_xref="Refseq:GI:1373262376|GI:1373285628|EMBL-CDS:AVS92
                     397.1"
                     /translation="MTIQEEAILTCRDAFAELASPACLHDPYPFMHQLRTHDPVHRAAS
                     GLFLLSRHADIFWVLKATGDAFRGPAPGELARYYPRAATSLSLQLLASTLAMKEPPTHT
                     RLRRLISRDFTLRQIDVLRPDIVRIIEERLDGMAPALERGEAVDLHREFSLAVSMLGFA
                     RLFGMPRDDMSRIAAGIGAILEGLSPRASDAQLAAADAASAGAQAYFEELIACKRAAPQ
                     DDLVSLLVGAHDGDADTLSDPELTSMLWGMLLGGFATTAATMDHAALAMISQPGAQRWL
                     QGDAAGVKAFVEEVLRHQAPAMFSSIPRIAQRDIELGGVVIPKDSDVRVLIAAGNRDPD
                     AFPDPDRFDPARFLGTSPGFSTDGQVMLSFGHGIHFCVGAQLARVEVAEGLSRLHARFP
                     ALSLAGTPVREPSAFLRTFRALPVRLQTPGG*"
     primer_bind     2596..2611
                     /label=seq1_CYP10
     primer_bind     3281..3298
                     /label=seq2_CYP10
     terminator      3450..3496
                     /label=L3S3P41
     misc_feature    3492
                     /label=C to G
     misc_feature    3506..3509
                     /label=4bp_scar6
     promoter        3525..3606
                     /label=Constitutive Promoter + RBS (DNA2.0 Vector)
                     /note="Geneious type: promoter"
     promoter        3543..3548
                     /label=-35 promoter
                     /label=-35
     misc_feature    3544
                     /label=C
                     /note="Geneious type: Editing History Replacement"
     promoter        3566..3571
                     /label=-10 promoter
                     /label=-10
     CDS             3607..4218
                     /label=CymR
     misc_feature    3904..3906
                     /label=E100D
                     /note="Geneious type: mutation"
     misc_feature    3906
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3934..3936
                     /label=S110
                     /note="Geneious type: mutation"
     misc_feature    3934
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3988..3990
                     /label=Q128L
                     /note="Geneious type: mutation"
     misc_feature    3989
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4117..4119
                     /label=A171
                     /note="Geneious type: mutation"
     misc_feature    4117
                     /label=G
                     /note="Geneious type: Editing History Replacement"
     terminator      4228..4482
                     /label=induction operon terminator region
ORIGIN
        1 actgactcgc tgcacgaggc agacctcagc gctagcggag tgtatactgg cttactatgt
       61 tggcactgat gagggtgtca gtgaagtgct tcatgtggca ggagaaaaaa ggctgcaccg
      121 gtgcgtcagc agaatatgtg atacaggata tattccgctt cctcgctcac tgactcgcta
      181 cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg
      241 gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc
      301 ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa
      361 acccgacagg actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc
      421 ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt
      481 ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa
      541 ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      601 gaaagacatg caaaagcacc actggcagca gccactggta attgatttag aggagttagt
      661 cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca agttttggtg actgcgctcc
      721 tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc
      781 ctgcaaggcg gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca
      841 agaagatcat cttattaagg ggtctgacgc tcagtggaac gaaaaatcaa tctaaagtat
      901 atatgagtaa acttggtctg acagttacct tagaaaaact catcgagcat caaatgaaac
      961 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     1021 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     1081 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     1141 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc
     1201 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     1261 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
     1321 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     1381 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg
     1441 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     1501 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     1561 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
     1621 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     1681 tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg ttgaatatgg
     1741 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat
     1801 atatttttat cttgtgcaat gtacatcaga gattttgaga cacaaccaat tattgaaggc
     1861 ctccctaacg gggggccttt ttttgtttct ggtctcccaa gcgcttaacg atcgttggct
     1921 gaacaaacag acaatctggt ctgtttgtat tatggaaaat ttttctgtat aatagattca
     1981 acaaacagac aatctggtct gtttgtatta tcagcggtca acgcatgtgc tttgcgttct
     2041 gatgagacag tgatgtcgaa accgcctcta caaataattt tgtttagagc tactacttaa
     2101 gggggaaatt gatgactatt caggaagagg caattttaac ttgtcgtgat gccttcgccg
     2161 aactggccag cccggcatgc ctgcatgatc catatccgtt tatgcatcaa ctgcgcaccc
     2221 acgatccggt gcatcgggcc gcgagtggtc tgttcttgct gtctcgtcac gccgatatct
     2281 tttgggttct gaaggcgacc ggtgatgctt ttcgcggacc ggctccgggt gagcttgccc
     2341 gttactatcc gagagcggcg actagtctgt cgcttcaact cttggcttca acactggcaa
     2401 tgaaagaacc tccaacgcat actcgattgc gccgtctgat ctcacgcgac tttaccctgc
     2461 gtcaaatcga tgtccttcgt ccagacattg ttcgcattat cgaagaacgt ttggatggca
     2521 tggcgccggc cttggaacgt ggtgaggccg tcgacttaca tcgggagttc tcacttgcgg
     2581 tgagtatgct gggttttgct cgtctgttcg gtatgcctcg agatgatatg tctcggatag
     2641 ccgccggtat cggtgcaatc ctcgaaggcc tcagtccgcg agcaagcgat gcgcaacttg
     2701 ccgcagcgga tgccgcaagc gcgggtgcac aggcatactt tgaggaactg attgcgtgta
     2761 aacgtgcagc gccgcaggac gacctggtca gcttattggt aggtgcgcac gacggcgacg
     2821 ctgatacgct gtctgatcct gagctcacct cgatgctctg gggtatgtta ctgggcggat
     2881 ttgctaccac ggcggcgacc atggatcatg ccgcgctggc tatgataagc caacctggtg
     2941 cgcagcgttg gctgcaaggt gatgccgccg gtgttaaggc tttcgttgaa gaagtcctcc
     3001 gtcaccaagc gccggccatg ttcagctcaa ttccgcgcat tgcgcaacgc gatatcgaac
     3061 tgggcggggt tgtaattcca aaggatagcg atgttcgcgt gttgatagcg gccggaaacc
     3121 gtgatccgga cgcctttccg gacccggata gatttgatcc ggcccgcttt ctgggcacct
     3181 cgccgggctt tagcactgat ggccaagtga tgctctcttt cgggcatgga atccacttct
     3241 gcgttggcgc acagctggca cgtgtggaag tggcggaggg attgtcacgg ttgcacgctc
     3301 gttttccggc actgagcctg gctggaactc cagttcgcga gccaagcgca tttctgcgca
     3361 cgtttcgcgc gctcccggtg cgcttacaga ctccgggcgg ttaataagga tgagctctac
     3421 aaataagcag aggtggttgt gttgcgaaaa aaaaaaaaaa acaccctaac gggtgttttt
     3481 ttttttttgg tgtccccacg tgtggcgctg gagaccgtcc aatggcggcg cgccatcgaa
     3541 tggtgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg
     3601 gtgaatatga gcccgaaacg tcgtacccag gcagaacgtg caatggaaac ccagggtaaa
     3661 ctgattgcag cagcactggg tgttctgcgt gaaaaaggtt atgcaggttt tcgtattgca
     3721 gatgttccgg gtgcagccgg tgttagccgt ggtgcacaga gccatcattt tccgaccaaa
     3781 ctggaactgc tgctggcaac ctttgaatgg ctgtatgagc agattaccga acgtagccgt
     3841 gcacgtctgg caaaactgaa accggaagat gatgttattc agcagatgct ggatgatgca
     3901 gcagattttt ttctggatga tgattttagc atcggcctgg atctgattgt tgcagcagat
     3961 cgtgatccgg cactgcgtga aggtattctg cgtaccgttg aacgtaatcg ttttgttgtt
     4021 gaagatatgt ggctgggtgt gctggtgagc cgtggtctga gccgtgatga tgccgaagat
     4081 attctgtggc tgatttttaa cagcgttcgt ggtctgacag ttcgtagcct gtggcagaaa
     4141 gataaagaac gttttgaacg tgtgcgtaat agcaccctgg aaattgcacg tgaacgttat
     4201 gcaaaattca aacgttgata aggatcctaa ttggtaacga atcagacaat tgacggctcg
     4261 agggagtagc atagggtttg cagaatccct gcttcgtcca tttgacaggc acattatgca
     4321 tcgatgataa gctgtcaaac atgagcagat cctctacgcc ggacgcatcg tggccggcat
     4381 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga
     4441 tcgggctcgc cacttcgggc tcatgagcaa atattttatc tgaggtgctt cctcgctc
//
