LOCUS       Exported                4653 bp ds-DNA     circular SYN 16-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Nov 17, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      22..858
                     /label=more stringent p15A
                     /label=more\stringent\p15A
     CDS             complement(930..1745)
                     /label=Kanamycin phosphotransferase
                     /label=Kanamycin\phosphotransferase
     terminator      1846..1891
                     /label=L3S3P21
                     /note="Geneious type: terminator"
     misc_feature    1898..1901
                     /label=4bp_scar5
     promoter        1922..2011
                     /label=pCymRC promoter
                     /label=pCymRC
     misc_feature    1922..1953
                     /label=CymO
                     /note="Geneious type: operator"
     promoter        1945..1950
                     /label=-35 promoter
                     /label=-35
     promoter        1968..1973
                     /label=-10 promoter
                     /label=-10
     misc_feature    1980..2011
                     /label=CymO
                     /note="Geneious type: operator"
     misc_feature    2013..2086
                     /label=RiboJ53
                     /note="Geneious type: riboj"
     RBS             2087..2113
                     /label=RBS
                     /note="strength = 50994"
     CDS             2114..3562
                     /codon_start=1
                     /product="CYP026"
                     /label=CYP026
                     /db_xref="UniProtKB/Swiss-Prot:A0A1C6NZN6"
                     /db_xref="Refseq:EMBL-CDS:SCK36502.1"
                     /translation="MRPGTRRTGMSRIGARLTGGPGRRTAADAPTDAPTDAATEAPVAP
                     GRVPLAGHAPRIHRDAPGFFRELRYHGPVTKIYIGPRQVHAVNSHEIVRELLTVQARAF
                     DKGAMFDALRVPLGDGLITAAGDRHLRRRRLMQPAFHHDRIAGYARIMSERSLARAAVW
                     EPGTTLDLVPEIHRLTLDILLRTLFADPQDSELRTAVKDWLSVKYHSMRLALSPLQAWA
                     ERLPLPAGWRPPHAGPLRRLVAVQHRIIETYRADGRDRGDLLSMLLATGGPDGGLTDAE
                     VTDELITLFLAGTGTVSASLAWTLHEISRRPDVQRRIHDELDAVLAGRPPGFEDLPALV
                     YTRQVLTEVLRLHPPSWLLMRRAVRPVTLGGVRLSRGAEVFFSPYALHRDPQLYENPDD
                     FDPERWSHDAAAKAPRHTYLPFGAGSRLCIGEDFAWTELTLALVAFTAHRRLTPAGSTP
                     VRTLVGTVLRPDRLPLTAHARPS*"
     primer_bind     2593..2607
                     /label=seq1_CYP26
     primer_bind     3323..3339
                     /label=seq2_CYP26
     terminator      3605..3651
                     /label=L3S3P41
     misc_feature    3647
                     /label=C to G
     misc_feature    3661..3664
                     /label=4bp_scar6
     promoter        3680..3761
                     /label=Constitutive Promoter + RBS (DNA2.0 Vector)
                     /note="Geneious type: promoter"
     promoter        3698..3703
                     /label=-35 promoter
                     /label=-35
     misc_feature    3699
                     /label=C
                     /note="Geneious type: Editing History Replacement"
     promoter        3721..3726
                     /label=-10 promoter
                     /label=-10
     CDS             3762..4373
                     /label=CymR
     misc_feature    4059..4061
                     /label=E100D
                     /note="Geneious type: mutation"
     misc_feature    4061
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4089..4091
                     /label=S110
                     /note="Geneious type: mutation"
     misc_feature    4089
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4143..4145
                     /label=Q128L
                     /note="Geneious type: mutation"
     misc_feature    4144
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4272..4274
                     /label=A171
                     /note="Geneious type: mutation"
     misc_feature    4272
                     /label=G
                     /note="Geneious type: Editing History Replacement"
     terminator      4383..4637
                     /label=induction operon terminator region
ORIGIN
        1 actgactcgc tgcacgaggc agacctcagc gctagcggag tgtatactgg cttactatgt
       61 tggcactgat gagggtgtca gtgaagtgct tcatgtggca ggagaaaaaa ggctgcaccg
      121 gtgcgtcagc agaatatgtg atacaggata tattccgctt cctcgctcac tgactcgcta
      181 cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg
      241 gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc
      301 ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa
      361 acccgacagg actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc
      421 ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt
      481 ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa
      541 ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      601 gaaagacatg caaaagcacc actggcagca gccactggta attgatttag aggagttagt
      661 cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca agttttggtg actgcgctcc
      721 tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc
      781 ctgcaaggcg gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca
      841 agaagatcat cttattaagg ggtctgacgc tcagtggaac gaaaaatcaa tctaaagtat
      901 atatgagtaa acttggtctg acagttacct tagaaaaact catcgagcat caaatgaaac
      961 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     1021 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     1081 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     1141 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc
     1201 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     1261 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
     1321 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     1381 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg
     1441 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     1501 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     1561 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
     1621 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     1681 tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg ttgaatatgg
     1741 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat
     1801 atatttttat cttgtgcaat gtacatcaga gattttgaga cacaaccaat tattgaaggc
     1861 ctccctaacg gggggccttt ttttgtttct ggtctcccaa gcgcttaacg atcgttggct
     1921 gaacaaacag acaatctggt ctgtttgtat tatggaaaat ttttctgtat aatagattca
     1981 acaaacagac aatctggtct gtttgtatta tcagcggtca acgcatgtgc tttgcgttct
     2041 gatgagacag tgatgtcgaa accgcctcta caaataattt tgtttaggcc caaatacaga
     2101 gaggggaggt tgtatgcgtc cgggcacaag acgtacgggc atgtctcgca ttggcgcgcg
     2161 tctgacggga ggcccgggcc gccgcacagc agctgatgca cctactgatg cgccgacgga
     2221 tgcggcaacc gaagctccag tggcacctgg ccgcgttcct ttggcggggc atgccccacg
     2281 gattcaccgt gatgcaccgg ggttcttcag agagttacgg tatcatggac cggtcactaa
     2341 gatctacatt ggaccgcgcc aggtacatgc agtcaatagc cacgaaatag tccgagagct
     2401 gctgacggtt caggctcgtg cgttcgacaa aggcgcaatg ttcgatgcgt tacgcgttcc
     2461 gttgggtgat ggcttaatta ccgccgccgg ggatcgccat ttacgccgcc gtcgtttaat
     2521 gcagcctgcg tttcaccatg accgcatcgc gggttatgcg cgcattatga gcgaacgttc
     2581 actggcccgt gcagctgttt gggagccggg cacgactctc gatctggtgc cggagatcca
     2641 ccgtctgacc ctggacattc tgctgagaac tctgtttgcc gatccgcagg atagcgaact
     2701 gcggaccgcg gtcaaagact ggttaagtgt gaaataccat tcgatgagat tggcccttag
     2761 tccgttacaa gcctgggctg aacgtctgcc ccttccagca ggatggcgtc caccgcatgc
     2821 cggcccactc cgtcgacttg tcgcggttca gcatcgtatt attgagactt accgcgcaga
     2881 cggtcgcgat cgtggagatc tcctgtcgat gctgcttgca accggcggtc cagacggcgg
     2941 gctgaccgat gcagaagtga cagatgaact cattaccctt tttctcgcag ggactggcac
     3001 cgtgagtgcc agcctggcct ggacccttca cgagattagc cgccggcctg atgttcagcg
     3061 gcgcattcac gatgagctgg atgcggttct ggctggacgg cctccaggtt ttgaagatct
     3121 gccggcttta gtatacacgc gccaggtcct gacggaggtg ctgcgtctgc atccgcctag
     3181 ctggttactg atgcgtcgtg ccgttcggcc agttacgctg ggtggcgtac gcctgagccg
     3241 cggcgctgag gtgttctttt ccccttatgc gctgcaccgc gatccgcagc tgtacgaaaa
     3301 tccggatgat tttgatccgg aacgttggtc gcatgatgcg gcggcaaaag cgccgcgtca
     3361 cacctatctg ccgtttggag cgggttctcg actgtgcatt ggtgaagatt tcgcgtggac
     3421 ggaattaacg ttggccttgg tggcatttac ggcccaccgt cgcttaacgc cggccggtag
     3481 tacgccagtg cgcactttag ttggtaccgt attacgtcca gatcgtctgc cgttaaccgc
     3541 gcacgcacgt ccaagctaat aaggatgagc tctacaaata agcagaggtg gttgtgttgc
     3601 gaaaaaaaaa aaaaaacacc ctaacgggtg tttttttttt tttggtgtcc ccacgtgtgg
     3661 cgctggagac cgtccaatgg cggcgcgcca tcgaatggtg caaaaccttt cgcggtatgg
     3721 catgatagcg cccggaagag agtcaattca gggtggtgaa tatgagcccg aaacgtcgta
     3781 cccaggcaga acgtgcaatg gaaacccagg gtaaactgat tgcagcagca ctgggtgttc
     3841 tgcgtgaaaa aggttatgca ggttttcgta ttgcagatgt tccgggtgca gccggtgtta
     3901 gccgtggtgc acagagccat cattttccga ccaaactgga actgctgctg gcaacctttg
     3961 aatggctgta tgagcagatt accgaacgta gccgtgcacg tctggcaaaa ctgaaaccgg
     4021 aagatgatgt tattcagcag atgctggatg atgcagcaga tttttttctg gatgatgatt
     4081 ttagcatcgg cctggatctg attgttgcag cagatcgtga tccggcactg cgtgaaggta
     4141 ttctgcgtac cgttgaacgt aatcgttttg ttgttgaaga tatgtggctg ggtgtgctgg
     4201 tgagccgtgg tctgagccgt gatgatgccg aagatattct gtggctgatt tttaacagcg
     4261 ttcgtggtct gacagttcgt agcctgtggc agaaagataa agaacgtttt gaacgtgtgc
     4321 gtaatagcac cctggaaatt gcacgtgaac gttatgcaaa attcaaacgt tgataaggat
     4381 cctaattggt aacgaatcag acaattgacg gctcgaggga gtagcatagg gtttgcagaa
     4441 tccctgcttc gtccatttga caggcacatt atgcatcgat gataagctgt caaacatgag
     4501 cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct
     4561 ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg
     4621 agcaaatatt ttatctgagg tgcttcctcg ctc
//
