LOCUS       Exported                4505 bp ds-DNA     circular SYN 16-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Nov 17, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      22..858
                     /label=more stringent p15A
                     /label=more\stringent\p15A
     CDS             complement(930..1745)
                     /label=Kanamycin phosphotransferase
                     /label=Kanamycin\phosphotransferase
     terminator      1846..1891
                     /label=L3S3P21
                     /note="Geneious type: terminator"
     misc_feature    1898..1901
                     /label=4bp_scar5
     promoter        1922..2011
                     /label=pCymRC promoter
                     /label=pCymRC
     misc_feature    1922..1953
                     /label=CymO
                     /note="Geneious type: operator"
     promoter        1945..1950
                     /label=-35 promoter
                     /label=-35
     promoter        1968..1973
                     /label=-10 promoter
                     /label=-10
     misc_feature    1980..2011
                     /label=CymO
                     /note="Geneious type: operator"
     misc_feature    2013..2086
                     /label=RiboJ53
                     /note="Geneious type: riboj"
     RBS             2087..2118
                     /label=RBS
                     /note="strength = 53298"
     CDS             2119..3414
                     /codon_start=1
                     /product="CYP034"
                     /label=CYP034
                     /db_xref="UniProtKB/Swiss-Prot:D8L2V0"
                     /db_xref="Refseq:GI:225791076|EMBL-CDS:ACO31280.1"
                     /translation="MSPPPSGAGDGTSCAPRPPSGPLPIKGRDVTDASSHPAPLPRARR
                     CPLDPPEEYAALRRTDPVSRLAFPDGSAGWLVTRYDDVRTVLTDPRFSARGDLVTSPVA
                     SQLRRRDAPAPGMFARMDPPDHTRYRRLLARHFHVRRVRALVPAIERIVADRLDALRRA
                     GPPADLVEIFALPVPTLVICELLGVPYEDRAAFQSWTASMVSVDSTREESDAAVAALAG
                     YVRSLVVAKRGVPAHDLLADLAADGELTDEETANIGLSVLVAGHETTANMLSLGAFALL
                     RHPEELAAFRADPGLTEQAVEELLRYLTIPQFGRERAALEDVVLGGRTLAAGEVVVASL
                     LSANRDPGRFDDPDTLDLRRPSAGHLAFGHGIHQCIGQQLAREQLRAGLRALFTQGPTL
                     RLAVPPEEVPMCEDSLNYGVRRLPVTWGTGR*"
     primer_bind     2597..2612
                     /label=seq1_CYP34
     primer_bind     3274..3290
                     /label=seq2_CYP34
     terminator      3457..3503
                     /label=L3S3P41
     misc_feature    3499
                     /label=C to G
     misc_feature    3513..3516
                     /label=4bp_scar6
     promoter        3532..3613
                     /label=Constitutive Promoter + RBS (DNA2.0 Vector)
                     /note="Geneious type: promoter"
     promoter        3550..3555
                     /label=-35 promoter
                     /label=-35
     misc_feature    3551
                     /label=C
                     /note="Geneious type: Editing History Replacement"
     promoter        3573..3578
                     /label=-10 promoter
                     /label=-10
     CDS             3614..4225
                     /label=CymR
     misc_feature    3911..3913
                     /label=E100D
                     /note="Geneious type: mutation"
     misc_feature    3913
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3941..3943
                     /label=S110
                     /note="Geneious type: mutation"
     misc_feature    3941
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3995..3997
                     /label=Q128L
                     /note="Geneious type: mutation"
     misc_feature    3996
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4124..4126
                     /label=A171
                     /note="Geneious type: mutation"
     misc_feature    4124
                     /label=G
                     /note="Geneious type: Editing History Replacement"
     terminator      4235..4489
                     /label=induction operon terminator region
ORIGIN
        1 actgactcgc tgcacgaggc agacctcagc gctagcggag tgtatactgg cttactatgt
       61 tggcactgat gagggtgtca gtgaagtgct tcatgtggca ggagaaaaaa ggctgcaccg
      121 gtgcgtcagc agaatatgtg atacaggata tattccgctt cctcgctcac tgactcgcta
      181 cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg
      241 gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc
      301 ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa
      361 acccgacagg actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc
      421 ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt
      481 ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa
      541 ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      601 gaaagacatg caaaagcacc actggcagca gccactggta attgatttag aggagttagt
      661 cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca agttttggtg actgcgctcc
      721 tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc
      781 ctgcaaggcg gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca
      841 agaagatcat cttattaagg ggtctgacgc tcagtggaac gaaaaatcaa tctaaagtat
      901 atatgagtaa acttggtctg acagttacct tagaaaaact catcgagcat caaatgaaac
      961 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     1021 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     1081 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     1141 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc
     1201 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     1261 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
     1321 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     1381 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg
     1441 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     1501 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     1561 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
     1621 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     1681 tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg ttgaatatgg
     1741 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat
     1801 atatttttat cttgtgcaat gtacatcaga gattttgaga cacaaccaat tattgaaggc
     1861 ctccctaacg gggggccttt ttttgtttct ggtctcccaa gcgcttaacg atcgttggct
     1921 gaacaaacag acaatctggt ctgtttgtat tatggaaaat ttttctgtat aatagattca
     1981 acaaacagac aatctggtct gtttgtatta tcagcggtca acgcatgtgc tttgcgttct
     2041 gatgagacag tgatgtcgaa accgcctcta caaataattt tgtttaggaa cacaccagta
     2101 agctatagga ggtatttaat gagtccgcca ccgagtggtg ccggggatgg tacatcctgc
     2161 gcgccccgtc ctccatcagg gcctctcccg attaaaggtc gtgatgtgac cgatgctagc
     2221 agccatccgg cgcctttacc gcgtgcgcgc cgatgtccgt tagacccacc agaggaatac
     2281 gccgccttgc gtcggaccga tccggtatcc cgtctggcat ttcccgatgg ttccgctggg
     2341 tggctggtga cccgttatga tgacgtgcga accgtgctga ccgatccgcg ctttagcgct
     2401 cggggagatc tcgttacctc gccggtagcc agccagttac ggcgccgtga tgcaccagcg
     2461 ccgggcatgt ttgctcgtat ggaccctccg gaccatactc gctatcgacg tctgttagcg
     2521 cgccactttc atgttcgtcg ggtccgcgca ctggtgcccg caatcgaacg catcgttgcc
     2581 gatcgcctcg atgcgcttcg tcgtgcaggt ccgccggccg atttagtgga aattttcgcc
     2641 ctgcctgtgc ccactctggt catttgcgaa ctgctgggtg tgccgtatga ggaccgagcg
     2701 gcgttccaga gctggacagc atctatggtt tcagtggaca gcacgcggga agaatctgat
     2761 gcggccgtgg ccgctctggc gggctatgtt cgctcactgg ttgtggcgaa acggggagtg
     2821 ccggcgcatg atcttcttgc cgacttagcc gcggatggtg aattgacgga tgaagaaacc
     2881 gccaacattg gccttagcgt acttgtggct ggccatgaaa ccaccgctaa tatgttgtcg
     2941 ttgggagctt tcgcactttt gcgccatccc gaggaactgg ctgccttccg tgccgatccg
     3001 ggtctgaccg agcaagctgt ggaagaactg ttgcgctatc tgaccatccc ccagttcggg
     3061 cgtgagcgcg ctgcactcga agatgtcgtg ctgggaggcc gcaccttggc cgccggtgaa
     3121 gttgtggtcg cttcactgtt gagcgcaaat cgcgacccgg ggcgctttga cgatccggat
     3181 accctggatc tgcgtcggcc atccgccggc cacctggcat ttggtcacgg aatccaccag
     3241 tgcataggac aacaactggc acgcgaacag ctcagagcgg gcttacgtgc tctgttcacc
     3301 caggggccca ctttacggtt ggcagttccc ccagaagaag taccaatgtg cgaggatagc
     3361 cttaattatg gtgttcgtcg cttacctgtc acgtggggta caggtcgcta ataaggatga
     3421 gctctacaaa taagcagagg tggttgtgtt gcgaaaaaaa aaaaaaaaca ccctaacggg
     3481 tgtttttttt tttttggtgt ccccacgtgt ggcgctggag accgtccaat ggcggcgcgc
     3541 catcgaatgg tgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt
     3601 cagggtggtg aatatgagcc cgaaacgtcg tacccaggca gaacgtgcaa tggaaaccca
     3661 gggtaaactg attgcagcag cactgggtgt tctgcgtgaa aaaggttatg caggttttcg
     3721 tattgcagat gttccgggtg cagccggtgt tagccgtggt gcacagagcc atcattttcc
     3781 gaccaaactg gaactgctgc tggcaacctt tgaatggctg tatgagcaga ttaccgaacg
     3841 tagccgtgca cgtctggcaa aactgaaacc ggaagatgat gttattcagc agatgctgga
     3901 tgatgcagca gatttttttc tggatgatga ttttagcatc ggcctggatc tgattgttgc
     3961 agcagatcgt gatccggcac tgcgtgaagg tattctgcgt accgttgaac gtaatcgttt
     4021 tgttgttgaa gatatgtggc tgggtgtgct ggtgagccgt ggtctgagcc gtgatgatgc
     4081 cgaagatatt ctgtggctga tttttaacag cgttcgtggt ctgacagttc gtagcctgtg
     4141 gcagaaagat aaagaacgtt ttgaacgtgt gcgtaatagc accctggaaa ttgcacgtga
     4201 acgttatgca aaattcaaac gttgataagg atcctaattg gtaacgaatc agacaattga
     4261 cggctcgagg gagtagcata gggtttgcag aatccctgct tcgtccattt gacaggcaca
     4321 ttatgcatcg atgataagct gtcaaacatg agcagatcct ctacgccgga cgcatcgtgg
     4381 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
     4441 gggaagatcg ggctcgccac ttcgggctca tgagcaaata ttttatctga ggtgcttcct
     4501 cgctc
//
