LOCUS       Exported                4394 bp ds-DNA     circular SYN 16-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Nov 17, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      22..858
                     /label=more stringent p15A
                     /label=more\stringent\p15A
     CDS             complement(930..1745)
                     /label=Kanamycin phosphotransferase
                     /label=Kanamycin\phosphotransferase
     terminator      1846..1891
                     /label=L3S3P21
                     /note="Geneious type: terminator"
     misc_feature    1898..1901
                     /label=4bp_scar5
     promoter        1922..2011
                     /label=pCymRC promoter
                     /label=pCymRC
     misc_feature    1922..1953
                     /label=CymO
                     /note="Geneious type: operator"
     promoter        1945..1950
                     /label=-35 promoter
                     /label=-35
     promoter        1968..1973
                     /label=-10 promoter
                     /label=-10
     misc_feature    1980..2011
                     /label=CymO
                     /note="Geneious type: operator"
     misc_feature    2013..2086
                     /label=RiboJ53
                     /note="Geneious type: riboj"
     RBS             2087..2115
                     /label=RBS
                     /note="strength = 46600"
     CDS             2116..3303
                     /codon_start=1
                     /product="CYP037"
                     /label=CYP037
                     /db_xref="UniProtKB/Swiss-Prot:A0A1I5LUC1"
                     /db_xref="Refseq:EMBL-CDS:SFP00928.1"
                     /translation="MTAAEQQFCPFDVGEEFRRLREDDPISRIELPSGTPAWFVARHAD
                     ARHVLTDERLSSVDASRLMRPGAGDDQVAPPGPGVLLFHDPPEHTRLRRMLAGNFTVRR
                     MNQLRPMVESIVNAQLDEMARAGSSTDFVRSFALPVPSLVICELLGVPYADRAEFQRRT
                     AAQVDLAMGEEHAAALWAESTEYMARLVVDKRANPTDDLLGRLVAEHGAELSDAELAGI
                     GVFLLGAGYETTANMLGLGTLLLLRHPEQLARLRDSTGDGTAVDRAVEELLRILTVVNN
                     AAMRQAKEDVTVGGQLIRAGEFVLVSLGSANRDDAVHERPDEFDIGRAPVGHMAFGHGI
                     HHCLGAPLARLEMQVAFPALLKRFPALRLDVPFDEIEYLPRSLVHGVRALPVAW*"
     primer_bind     2586..2600
                     /label=seq1_CYP37
     primer_bind     3232..3252
                     /label=seq2_CYP37
     terminator      3346..3392
                     /label=L3S3P41
     misc_feature    3388
                     /label=C to G
     misc_feature    3402..3405
                     /label=4bp_scar6
     promoter        3421..3502
                     /label=Constitutive Promoter + RBS (DNA2.0 Vector)
                     /note="Geneious type: promoter"
     promoter        3439..3444
                     /label=-35 promoter
                     /label=-35
     misc_feature    3440
                     /label=C
                     /note="Geneious type: Editing History Replacement"
     promoter        3462..3467
                     /label=-10 promoter
                     /label=-10
     CDS             3503..4114
                     /label=CymR
     misc_feature    3800..3802
                     /label=E100D
                     /note="Geneious type: mutation"
     misc_feature    3802
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3830..3832
                     /label=S110
                     /note="Geneious type: mutation"
     misc_feature    3830
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3884..3886
                     /label=Q128L
                     /note="Geneious type: mutation"
     misc_feature    3885
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4013..4015
                     /label=A171
                     /note="Geneious type: mutation"
     misc_feature    4013
                     /label=G
                     /note="Geneious type: Editing History Replacement"
     terminator      4124..4378
                     /label=induction operon terminator region
ORIGIN
        1 actgactcgc tgcacgaggc agacctcagc gctagcggag tgtatactgg cttactatgt
       61 tggcactgat gagggtgtca gtgaagtgct tcatgtggca ggagaaaaaa ggctgcaccg
      121 gtgcgtcagc agaatatgtg atacaggata tattccgctt cctcgctcac tgactcgcta
      181 cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg
      241 gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc
      301 ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa
      361 acccgacagg actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc
      421 ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt
      481 ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa
      541 ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      601 gaaagacatg caaaagcacc actggcagca gccactggta attgatttag aggagttagt
      661 cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca agttttggtg actgcgctcc
      721 tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc
      781 ctgcaaggcg gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca
      841 agaagatcat cttattaagg ggtctgacgc tcagtggaac gaaaaatcaa tctaaagtat
      901 atatgagtaa acttggtctg acagttacct tagaaaaact catcgagcat caaatgaaac
      961 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     1021 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     1081 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     1141 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc
     1201 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     1261 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
     1321 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     1381 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg
     1441 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     1501 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     1561 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
     1621 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     1681 tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg ttgaatatgg
     1741 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat
     1801 atatttttat cttgtgcaat gtacatcaga gattttgaga cacaaccaat tattgaaggc
     1861 ctccctaacg gggggccttt ttttgtttct ggtctcccaa gcgcttaacg atcgttggct
     1921 gaacaaacag acaatctggt ctgtttgtat tatggaaaat ttttctgtat aatagattca
     1981 acaaacagac aatctggtct gtttgtatta tcagcggtca acgcatgtgc tttgcgttct
     2041 gatgagacag tgatgtcgaa accgcctcta caaataattt tgtttaggtc ccgagagaga
     2101 ttataaggag gttctatgac tgcagcagag cagcagttct gtccgtttga cgtgggcgag
     2161 gagtttcgtc gcctccgcga ggacgacccg attagccgaa tcgagctgcc cagcggtact
     2221 ccggcgtggt tcgtcgcacg ccacgccgac gcaagacatg tgttgaccga tgaacgttta
     2281 tcttcagtag atgcaagtcg tctgatgcgt ccaggtgccg gggatgatca ggtagcgccg
     2341 cctggtccgg gtgtgctgct gtttcacgat ccgccggaac acacgcgcct ccgacgcatg
     2401 ctggcaggca attttacggt tagacgcatg aaccaattac gtcctatggt ggagtcaatc
     2461 gtcaacgcac agttagacga aatggcgcgc gcgggcagct cgacggactt tgtgcgttcc
     2521 ttcgcgctcc cggtgccctc cctggtaatt tgtgaattgc tcggggttcc gtatgctgat
     2581 cgcgctgaat tccaacggcg taccgcggct caggtggact tagctatggg tgaagaacac
     2641 gcggcggcgc tctgggctga atccaccgaa tacatggccc gtctggttgt agataaacgc
     2701 gccaacccga ccgacgacct tctgggccgc ttggttgccg aacatggagc agaactgtct
     2761 gatgcggagc tggccggaat tggtgttttc ttattgggcg ctgggtatga aacgacagca
     2821 aatatgctgg ggctcggtac actgttatta ctgagacacc cggagcagct cgcacgcctg
     2881 cgcgactcta cgggggatgg cacagcggtc gatcgtgcgg tggaggaact tctgcgtatt
     2941 ctgaccgtgg ttaacaacgc agcaatgcgc caggcaaagg aagatgttac cgtcggcgga
     3001 cagttaattc gcgccgggga atttgttctg gtgtccctgg gctccgctaa ccgcgatgac
     3061 gcagtgcacg aacgccccga cgaatttgac attggccgtg caccggtggg ccacatggcc
     3121 ttcggtcatg gcattcatca ctgtcttgga gccccgctgg cgcgcctcga gatgcaggtc
     3181 gcttttccgg ccctgctcaa acgctttccg gcgctgcggc tggacgttcc gttcgacgag
     3241 attgaatatc tgccgcgcag cttggtccat ggcgtacgtg cgctgccggt ggcttggtaa
     3301 taaggatgag ctctacaaat aagcagaggt ggttgtgttg cgaaaaaaaa aaaaaaacac
     3361 cctaacgggt gttttttttt ttttggtgtc cccacgtgtg gcgctggaga ccgtccaatg
     3421 gcggcgcgcc atcgaatggt gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga
     3481 gagtcaattc agggtggtga atatgagccc gaaacgtcgt acccaggcag aacgtgcaat
     3541 ggaaacccag ggtaaactga ttgcagcagc actgggtgtt ctgcgtgaaa aaggttatgc
     3601 aggttttcgt attgcagatg ttccgggtgc agccggtgtt agccgtggtg cacagagcca
     3661 tcattttccg accaaactgg aactgctgct ggcaaccttt gaatggctgt atgagcagat
     3721 taccgaacgt agccgtgcac gtctggcaaa actgaaaccg gaagatgatg ttattcagca
     3781 gatgctggat gatgcagcag atttttttct ggatgatgat tttagcatcg gcctggatct
     3841 gattgttgca gcagatcgtg atccggcact gcgtgaaggt attctgcgta ccgttgaacg
     3901 taatcgtttt gttgttgaag atatgtggct gggtgtgctg gtgagccgtg gtctgagccg
     3961 tgatgatgcc gaagatattc tgtggctgat ttttaacagc gttcgtggtc tgacagttcg
     4021 tagcctgtgg cagaaagata aagaacgttt tgaacgtgtg cgtaatagca ccctggaaat
     4081 tgcacgtgaa cgttatgcaa aattcaaacg ttgataagga tcctaattgg taacgaatca
     4141 gacaattgac ggctcgaggg agtagcatag ggtttgcaga atccctgctt cgtccatttg
     4201 acaggcacat tatgcatcga tgataagctg tcaaacatga gcagatcctc tacgccggac
     4261 gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca
     4321 tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcaaatat tttatctgag
     4381 gtgcttcctc gctc
//
