LOCUS       Exported                4440 bp ds-DNA     circular SYN 16-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Nov 17, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      22..858
                     /label=more stringent p15A
                     /label=more\stringent\p15A
     CDS             complement(930..1745)
                     /label=Kanamycin phosphotransferase
                     /label=Kanamycin\phosphotransferase
     terminator      1846..1891
                     /label=L3S3P21
                     /note="Geneious type: terminator"
     misc_feature    1898..1901
                     /label=4bp_scar5
     promoter        1922..2011
                     /label=pCymRC promoter
                     /label=pCymRC
     misc_feature    1922..1953
                     /label=CymO
                     /note="Geneious type: operator"
     promoter        1945..1950
                     /label=-35 promoter
                     /label=-35
     promoter        1968..1973
                     /label=-10 promoter
                     /label=-10
     misc_feature    1980..2011
                     /label=CymO
                     /note="Geneious type: operator"
     misc_feature    2013..2086
                     /label=RiboJ53
                     /note="Geneious type: riboj"
     RBS             2087..2110
                     /label=RBS
                     /note="strength = 53538"
     CDS             2111..3349
                     /codon_start=1
                     /product="CYP041"
                     /label=CYP041
                     /db_xref="UniProtKB/Swiss-Prot:A0A0K2ZXB3"
                     /db_xref="Refseq:EMBL-CDS:CTP90308.1"
                     /translation="MRVEGDRYDVIDMSVAQSQLDNASSSILQWDGVARVALPGNVVTW
                     AAGRHQTLKCILADQRFSRDWRHWRALQDGEIPEDHPLIGMCRLDNMVTAHGADHQRLR
                     GLLARSFAPSRIALLAPDIERRVDQLLAGIAARGSADLMQEFAVPLPASVIAELFGLPE
                     ENRAEIILLTNSLASTSASAAEVQQTRQRIPDFFRWLIALKRRRPGDDLASALIAARDS
                     GELVSDTELIDMLFMVLSAGFVTTTGVIGNGVLALLTHPQQLRLVLSGQVPWAQAIEEI
                     LRWGSSVTNLPFRYATQDVEIDGCTIRRGEAILMAFHAANRDERAFGPGAEAFDVTRQP
                     NPHLSFGQGPHFCLGAALARLELLYAFPALFARLHELALAVALEDIAYQPSYVIRCPQR
                     LPVTFRPSIARK*"
     primer_bind     2575..2595
                     /label=seq1_CYP41
     primer_bind     3273..3293
                     /label=seq2_CYP41
     terminator      3392..3438
                     /label=L3S3P41
     misc_feature    3434
                     /label=C to G
     misc_feature    3448..3451
                     /label=4bp_scar6
     promoter        3467..3548
                     /label=Constitutive Promoter + RBS (DNA2.0 Vector)
                     /note="Geneious type: promoter"
     promoter        3485..3490
                     /label=-35 promoter
                     /label=-35
     misc_feature    3486
                     /label=C
                     /note="Geneious type: Editing History Replacement"
     promoter        3508..3513
                     /label=-10 promoter
                     /label=-10
     CDS             3549..4160
                     /label=CymR
     misc_feature    3846..3848
                     /label=E100D
                     /note="Geneious type: mutation"
     misc_feature    3848
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3876..3878
                     /label=S110
                     /note="Geneious type: mutation"
     misc_feature    3876
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3930..3932
                     /label=Q128L
                     /note="Geneious type: mutation"
     misc_feature    3931
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4059..4061
                     /label=A171
                     /note="Geneious type: mutation"
     misc_feature    4059
                     /label=G
                     /note="Geneious type: Editing History Replacement"
     terminator      4170..4424
                     /label=induction operon terminator region
ORIGIN
        1 actgactcgc tgcacgaggc agacctcagc gctagcggag tgtatactgg cttactatgt
       61 tggcactgat gagggtgtca gtgaagtgct tcatgtggca ggagaaaaaa ggctgcaccg
      121 gtgcgtcagc agaatatgtg atacaggata tattccgctt cctcgctcac tgactcgcta
      181 cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg
      241 gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc
      301 ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa
      361 acccgacagg actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc
      421 ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt
      481 ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa
      541 ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      601 gaaagacatg caaaagcacc actggcagca gccactggta attgatttag aggagttagt
      661 cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca agttttggtg actgcgctcc
      721 tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc
      781 ctgcaaggcg gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca
      841 agaagatcat cttattaagg ggtctgacgc tcagtggaac gaaaaatcaa tctaaagtat
      901 atatgagtaa acttggtctg acagttacct tagaaaaact catcgagcat caaatgaaac
      961 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     1021 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     1081 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     1141 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc
     1201 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     1261 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
     1321 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     1381 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg
     1441 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     1501 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     1561 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
     1621 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     1681 tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg ttgaatatgg
     1741 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat
     1801 atatttttat cttgtgcaat gtacatcaga gattttgaga cacaaccaat tattgaaggc
     1861 ctccctaacg gggggccttt ttttgtttct ggtctcccaa gcgcttaacg atcgttggct
     1921 gaacaaacag acaatctggt ctgtttgtat tatggaaaat ttttctgtat aatagattca
     1981 acaaacagac aatctggtct gtttgtatta tcagcggtca acgcatgtgc tttgcgttct
     2041 gatgagacag tgatgtcgaa accgcctcta caaataattt tgtttatttg tattgattaa
     2101 ggaggatctt atgcgtgtgg agggcgacag atatgacgtt atcgacatga gtgtggccca
     2161 gagccagctg gataatgcgt cctcaagcat tctgcagtgg gatggcgtcg cacgcgtggc
     2221 cttaccgggc aatgtggtta cgtgggccgc agggcgacac cagaccttga aatgtattct
     2281 ggcggatcag cgtttctccc gcgattggcg acattggcgc gctctccagg atggggagat
     2341 tccggaggat catcctctga ttggaatgtg tcgcttggat aacatggtga ctgcgcacgg
     2401 tgctgatcat cagcggctgc gtggcctgct ggcacgttcc ttcgcacctt cgcggatcgc
     2461 gttattagca ccagacatcg agcgtcgtgt tgaccagttg ttggcgggca ttgctgcgcg
     2521 tgggagcgcg gatctgatgc aggagttcgc cgttccgctg ccagcgagcg ttattgctga
     2581 gttatttgga ttgccggagg aaaatcgcgc agaaatcatt ctgctgacca atagcctggc
     2641 ctcgacaagc gcttctgcgg cagaagtgca gcaaacgcgc caacgcattc ctgacttctt
     2701 tcgctggctg attgcgctga aacgtcgtcg ccccggtgat gatctggcca gcgctctgat
     2761 tgcggcgcgc gattctggag aattagtgag cgatacggaa cttatagaca tgttgtttat
     2821 ggtactgtcg gcgggctttg tgaccacgac gggtgttatc ggaaacggcg tcctggcgtt
     2881 gcttacgcat ccccaacagc tgcgtctggt gctgagcgga caggtgccgt gggcacaggc
     2941 tatcgaagaa atcttacgct ggggcagctc ggtcaccaac ctgccgtttc ggtacgcaac
     3001 ccaggatgtt gaaatcgatg gctgcaccat ccgccgtggc gaggctatcc tcatggcgtt
     3061 tcacgctgcc aatcgtgacg aacgagcgtt tggacccggt gcagaagcct ttgatgttac
     3121 gcgtcaacct aacccgcacc tgagctttgg tcagggccca catttctgtt tgggcgctgc
     3181 actggcgcgc ctggaactgt tgtatgcctt cccagcgtta tttgcacgct tacacgagct
     3241 cgcgctggcg gttgccttag aggacattgc ataccaaccc agctatgtaa tccgttgccc
     3301 gcaacgcctg ccggtgacct ttcgtccaag cattgcccgt aaataataag gatgagctct
     3361 acaaataagc agaggtggtt gtgttgcgaa aaaaaaaaaa aaacacccta acgggtgttt
     3421 tttttttttt ggtgtcccca cgtgtggcgc tggagaccgt ccaatggcgg cgcgccatcg
     3481 aatggtgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt caattcaggg
     3541 tggtgaatat gagcccgaaa cgtcgtaccc aggcagaacg tgcaatggaa acccagggta
     3601 aactgattgc agcagcactg ggtgttctgc gtgaaaaagg ttatgcaggt tttcgtattg
     3661 cagatgttcc gggtgcagcc ggtgttagcc gtggtgcaca gagccatcat tttccgacca
     3721 aactggaact gctgctggca acctttgaat ggctgtatga gcagattacc gaacgtagcc
     3781 gtgcacgtct ggcaaaactg aaaccggaag atgatgttat tcagcagatg ctggatgatg
     3841 cagcagattt ttttctggat gatgatttta gcatcggcct ggatctgatt gttgcagcag
     3901 atcgtgatcc ggcactgcgt gaaggtattc tgcgtaccgt tgaacgtaat cgttttgttg
     3961 ttgaagatat gtggctgggt gtgctggtga gccgtggtct gagccgtgat gatgccgaag
     4021 atattctgtg gctgattttt aacagcgttc gtggtctgac agttcgtagc ctgtggcaga
     4081 aagataaaga acgttttgaa cgtgtgcgta atagcaccct ggaaattgca cgtgaacgtt
     4141 atgcaaaatt caaacgttga taaggatcct aattggtaac gaatcagaca attgacggct
     4201 cgagggagta gcatagggtt tgcagaatcc ctgcttcgtc catttgacag gcacattatg
     4261 catcgatgat aagctgtcaa acatgagcag atcctctacg ccggacgcat cgtggccggc
     4321 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
     4381 gatcgggctc gccacttcgg gctcatgagc aaatatttta tctgaggtgc ttcctcgctc
//
