LOCUS       Exported                4504 bp ds-DNA     circular SYN 16-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Nov 17, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      22..858
                     /label=more stringent p15A
                     /label=more\stringent\p15A
     CDS             complement(930..1745)
                     /label=Kanamycin phosphotransferase
                     /label=Kanamycin\phosphotransferase
     terminator      1846..1891
                     /label=L3S3P21
                     /note="Geneious type: terminator"
     misc_feature    1898..1901
                     /label=4bp_scar5
     promoter        1922..2011
                     /label=pCymRC promoter
                     /label=pCymRC
     misc_feature    1922..1953
                     /label=CymO
                     /note="Geneious type: operator"
     promoter        1945..1950
                     /label=-35 promoter
                     /label=-35
     promoter        1968..1973
                     /label=-10 promoter
                     /label=-10
     misc_feature    1980..2011
                     /label=CymO
                     /note="Geneious type: operator"
     misc_feature    2013..2086
                     /label=RiboJ53
                     /note="Geneious type: riboj"
     RBS             2087..2114
                     /label=RBS
                     /note="strength = 49427"
     CDS             2115..3413
                     /codon_start=1
                     /product="CYP051"
                     /label=CYP051
                     /db_xref="UniProtKB/Swiss-Prot:A0A4R7JD75"
                     /db_xref="Refseq:EMBL-CDS:TDT35620.1"
                     /translation="MSITPPAVTAVPLHGPEHAANPQRSYELLRLQGPVGLAEVAPGVV
                     VHLVTDYRAALDLLQDTDTWTKDTRSWLPQVPENSPIRPLVEWRPSLFFADGEEHAHLR
                     KVITDSFGLLDPYDVRVLTFRHADRLLQDFAADGTVDLVSQYAQQLPLMVFNALFGMGD
                     EYGPQLVRALSAMMDTDPEKKAEGAQSFGAYLQTLYQTKAAQRGHDLTSWFIDHPNGLN
                     QEEAINQILITLGAGNEPTANLIANTCVRMLSDDRYYGTLTTGSLPIQHAIDDALWNDS
                     PLANYSFHFPKEDITFHGKRIPAGSPVMVSYAAANTCPHAGVPSDGYRSGTKAHLAFAA
                     GPHTCPARDLALLIATAAIERLASYLPDIELAVSPDQLTYRDGAIHRTLTSLPARFTPL
                     TPDANGATPWSRNTSSPKTDTGPPQQQATASR*"
     primer_bind     2585..2599
                     /label=seq1_CYP51
     primer_bind     3276..3292
                     /label=seq2_CYP51
     terminator      3456..3502
                     /label=L3S3P41
     misc_feature    3498
                     /label=C to G
     misc_feature    3512..3515
                     /label=4bp_scar6
     promoter        3531..3612
                     /label=Constitutive Promoter + RBS (DNA2.0 Vector)
                     /note="Geneious type: promoter"
     promoter        3549..3554
                     /label=-35 promoter
                     /label=-35
     misc_feature    3550
                     /label=C
                     /note="Geneious type: Editing History Replacement"
     promoter        3572..3577
                     /label=-10 promoter
                     /label=-10
     CDS             3613..4224
                     /label=CymR
     misc_feature    3910..3912
                     /label=E100D
                     /note="Geneious type: mutation"
     misc_feature    3912
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3940..3942
                     /label=S110
                     /note="Geneious type: mutation"
     misc_feature    3940
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3994..3996
                     /label=Q128L
                     /note="Geneious type: mutation"
     misc_feature    3995
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4123..4125
                     /label=A171
                     /note="Geneious type: mutation"
     misc_feature    4123
                     /label=G
                     /note="Geneious type: Editing History Replacement"
     terminator      4234..4488
                     /label=induction operon terminator region
ORIGIN
        1 actgactcgc tgcacgaggc agacctcagc gctagcggag tgtatactgg cttactatgt
       61 tggcactgat gagggtgtca gtgaagtgct tcatgtggca ggagaaaaaa ggctgcaccg
      121 gtgcgtcagc agaatatgtg atacaggata tattccgctt cctcgctcac tgactcgcta
      181 cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg
      241 gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc
      301 ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa
      361 acccgacagg actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc
      421 ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt
      481 ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa
      541 ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      601 gaaagacatg caaaagcacc actggcagca gccactggta attgatttag aggagttagt
      661 cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca agttttggtg actgcgctcc
      721 tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc
      781 ctgcaaggcg gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca
      841 agaagatcat cttattaagg ggtctgacgc tcagtggaac gaaaaatcaa tctaaagtat
      901 atatgagtaa acttggtctg acagttacct tagaaaaact catcgagcat caaatgaaac
      961 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     1021 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     1081 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     1141 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc
     1201 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     1261 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
     1321 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     1381 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg
     1441 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     1501 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     1561 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
     1621 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     1681 tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg ttgaatatgg
     1741 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat
     1801 atatttttat cttgtgcaat gtacatcaga gattttgaga cacaaccaat tattgaaggc
     1861 ctccctaacg gggggccttt ttttgtttct ggtctcccaa gcgcttaacg atcgttggct
     1921 gaacaaacag acaatctggt ctgtttgtat tatggaaaat ttttctgtat aatagattca
     1981 acaaacagac aatctggtct gtttgtatta tcagcggtca acgcatgtgc tttgcgttct
     2041 gatgagacag tgatgtcgaa accgcctcta caaataattt tgtttatggt ctagcctgtc
     2101 taggaggtca agtaatgagc atcacacccc cggccgtaac cgcggtgccg ttgcatggtc
     2161 ctgagcatgc cgctaaccca cagcgctctt atgaactgtt aagactccag ggccctgttg
     2221 gcctggcgga agtagcgccg ggagtggtgg tccatctggt aaccgactac cgggctgcat
     2281 tagacctgtt acaagacacc gatacctgga ccaaagatac ccgcagttgg ctgccgcagg
     2341 ttcctgaaaa ctctcccatt cgcccattgg tagaatggcg cccaagcctc ttctttgcgg
     2401 atggcgaaga acatgcgcat ttgcgaaaag tgattacaga ttcgttcggc cttcttgatc
     2461 cttacgatgt tcgcgttctg acctttcgcc acgctgatcg cttactgcaa gatttcgcag
     2521 cggacggaac cgttgatctg gttagccagt atgctcagca gctgccgctg atggttttta
     2581 atgcactgtt cggcatgggt gatgagtatg gcccgcagct tgtgcgcgct ttaagcgcaa
     2641 tgatggatac agatcctgaa aagaaagccg aaggcgcaca gtcattcggg gcctatctgc
     2701 agacactgta ccagacaaaa gcggcgcaaa gagggcatga tctgacctcc tggtttatcg
     2761 atcatccgaa tggtttgaac caggaagaag caattaacca gattctgata acactgggcg
     2821 cagggaatga gcctaccgcc aacctgatcg cgaacacttg tgtgcggatg ctctccgatg
     2881 atagatatta tggcactctg acaacggggt cattaccgat tcagcatgcg attgatgatg
     2941 ctttatggaa tgatagcccg ctggccaact actcttttca ttttccaaag gaggacatta
     3001 cattccatgg caaacgtata ccagccggaa gcccggtgat ggttagttat gcggctgcta
     3061 atacgtgtcc tcatgccggg gtcccgagtg acggttatcg gagcggtacc aaagcgcact
     3121 tagcatttgc tgccggcccg catacgtgtc cggcacgcga cttagctctg ttgatcgcga
     3181 cggcggctat cgaacgtctg gcatcatatc tcccagacat tgaactggcg gtaagcccgg
     3241 accagttaac ctaccgggac ggcgcgatcc atcgcacact tacgtcattg ccagcgagat
     3301 ttactccgct caccccggat gcgaacggcg caaccccgtg gagtcgtaat acttcatccc
     3361 ctaaaaccga tactggtccg ccacagcaac aagcgacagc ttcgcgctaa taaggatgag
     3421 ctctacaaat aagcagaggt ggttgtgttg cgaaaaaaaa aaaaaaacac cctaacgggt
     3481 gttttttttt ttttggtgtc cccacgtgtg gcgctggaga ccgtccaatg gcggcgcgcc
     3541 atcgaatggt gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc
     3601 agggtggtga atatgagccc gaaacgtcgt acccaggcag aacgtgcaat ggaaacccag
     3661 ggtaaactga ttgcagcagc actgggtgtt ctgcgtgaaa aaggttatgc aggttttcgt
     3721 attgcagatg ttccgggtgc agccggtgtt agccgtggtg cacagagcca tcattttccg
     3781 accaaactgg aactgctgct ggcaaccttt gaatggctgt atgagcagat taccgaacgt
     3841 agccgtgcac gtctggcaaa actgaaaccg gaagatgatg ttattcagca gatgctggat
     3901 gatgcagcag atttttttct ggatgatgat tttagcatcg gcctggatct gattgttgca
     3961 gcagatcgtg atccggcact gcgtgaaggt attctgcgta ccgttgaacg taatcgtttt
     4021 gttgttgaag atatgtggct gggtgtgctg gtgagccgtg gtctgagccg tgatgatgcc
     4081 gaagatattc tgtggctgat ttttaacagc gttcgtggtc tgacagttcg tagcctgtgg
     4141 cagaaagata aagaacgttt tgaacgtgtg cgtaatagca ccctggaaat tgcacgtgaa
     4201 cgttatgcaa aattcaaacg ttgataagga tcctaattgg taacgaatca gacaattgac
     4261 ggctcgaggg agtagcatag ggtttgcaga atccctgctt cgtccatttg acaggcacat
     4321 tatgcatcga tgataagctg tcaaacatga gcagatcctc tacgccggac gcatcgtggc
     4381 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
     4441 ggaagatcgg gctcgccact tcgggctcat gagcaaatat tttatctgag gtgcttcctc
     4501 gctc
//
