LOCUS       Exported                4314 bp ds-DNA     circular SYN 16-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Nov 17, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      22..858
                     /label=more stringent p15A
                     /label=more\stringent\p15A
     CDS             complement(930..1745)
                     /label=Kanamycin phosphotransferase
                     /label=Kanamycin\phosphotransferase
     terminator      1846..1891
                     /label=L3S3P21
                     /note="Geneious type: terminator"
     misc_feature    1898..1901
                     /label=4bp_scar5
     promoter        1922..2011
                     /label=pCymRC promoter
                     /label=pCymRC
     misc_feature    1922..1953
                     /label=CymO
                     /note="Geneious type: operator"
     promoter        1945..1950
                     /label=-35 promoter
                     /label=-35
     promoter        1968..1973
                     /label=-10 promoter
                     /label=-10
     misc_feature    1980..2011
                     /label=CymO
                     /note="Geneious type: operator"
     misc_feature    2013..2086
                     /label=RiboJ53
                     /note="Geneious type: riboj"
     RBS             2087..2116
                     /label=RBS
                     /note="strength = 51960"
     CDS             2117..3223
                     /codon_start=1
                     /product="CYP058"
                     /label=CYP058
                     /db_xref="UniProtKB/Swiss-Prot:A0A316I275"
                     /db_xref="Refseq:EMBL-CDS:PWK86591.1"
                     /translation="MNRTELRFALQLIEFLDSVGDFEDGLVKLRDRRWLVWHPDVMAAL
                     FRHDRALRHPPSRTLSPLLGNRSVLWLDGEEHTAYRRTLAPVLRGSRYHGLIGEMVERS
                     LAELPSGDPVSLAGWTRKLTLRIMGRIIFGVDDDELLTAFTRWLDRRLGSRVRTLAHRC
                     FTPGTTRFDPHLDRLLLQRARSAEPPALAACLPPDDLRDQIVSLLFAGHETTASATAWT
                     VYWIDRTPGLAEQLLTDETLLDAAITESLRLSPPATLAGNRMSDDGMVITPSIYLAHRR
                     AATYENPHTFDPSRKPPPPGAFFPFGGGVRHCPGKDLALTEIRMIVTALLRHRRIRLNT
                     PRSCTPQLRGAAMAPPPSLAMVATCRR*"
     primer_bind     2598..2613
                     /label=seq1_CYP58
     primer_bind     3187..3204
                     /label=seq2_CYP58
     terminator      3266..3312
                     /label=L3S3P41
     misc_feature    3308
                     /label=C to G
     misc_feature    3322..3325
                     /label=4bp_scar6
     promoter        3341..3422
                     /label=Constitutive Promoter + RBS (DNA2.0 Vector)
                     /note="Geneious type: promoter"
     promoter        3359..3364
                     /label=-35 promoter
                     /label=-35
     misc_feature    3360
                     /label=C
                     /note="Geneious type: Editing History Replacement"
     promoter        3382..3387
                     /label=-10 promoter
                     /label=-10
     CDS             3423..4034
                     /label=CymR
     misc_feature    3720..3722
                     /label=E100D
                     /note="Geneious type: mutation"
     misc_feature    3722
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3750..3752
                     /label=S110
                     /note="Geneious type: mutation"
     misc_feature    3750
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3804..3806
                     /label=Q128L
                     /note="Geneious type: mutation"
     misc_feature    3805
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3933..3935
                     /label=A171
                     /note="Geneious type: mutation"
     misc_feature    3933
                     /label=G
                     /note="Geneious type: Editing History Replacement"
     terminator      4044..4298
                     /label=induction operon terminator region
ORIGIN
        1 actgactcgc tgcacgaggc agacctcagc gctagcggag tgtatactgg cttactatgt
       61 tggcactgat gagggtgtca gtgaagtgct tcatgtggca ggagaaaaaa ggctgcaccg
      121 gtgcgtcagc agaatatgtg atacaggata tattccgctt cctcgctcac tgactcgcta
      181 cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg
      241 gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc
      301 ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa
      361 acccgacagg actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc
      421 ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt
      481 ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa
      541 ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      601 gaaagacatg caaaagcacc actggcagca gccactggta attgatttag aggagttagt
      661 cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca agttttggtg actgcgctcc
      721 tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc
      781 ctgcaaggcg gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca
      841 agaagatcat cttattaagg ggtctgacgc tcagtggaac gaaaaatcaa tctaaagtat
      901 atatgagtaa acttggtctg acagttacct tagaaaaact catcgagcat caaatgaaac
      961 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     1021 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     1081 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     1141 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc
     1201 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     1261 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
     1321 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     1381 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg
     1441 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     1501 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     1561 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
     1621 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     1681 tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg ttgaatatgg
     1741 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat
     1801 atatttttat cttgtgcaat gtacatcaga gattttgaga cacaaccaat tattgaaggc
     1861 ctccctaacg gggggccttt ttttgtttct ggtctcccaa gcgcttaacg atcgttggct
     1921 gaacaaacag acaatctggt ctgtttgtat tatggaaaat ttttctgtat aatagattca
     1981 acaaacagac aatctggtct gtttgtatta tcagcggtca acgcatgtgc tttgcgttct
     2041 gatgagacag tgatgtcgaa accgcctcta caaataattt tgtttagagt aagacgaaaa
     2101 aaccaggagc ccgcttatga accgtactga actgcgcttt gcattacaac tgatcgagtt
     2161 tttagattct gtgggtgatt tcgaagatgg cctggttaaa ttgcgcgacc gtcgttggtt
     2221 ggtgtggcat ccggatgtca tggcggcctt gttccgccac gaccgagcgc ttagacatcc
     2281 gcccagtcgc acacttagcc cgctcttggg aaacagatcc gtgttgtggt tggatggcga
     2341 agaacatact gcataccgtc gcacgctcgc tccagtatta cgcggttcac gttaccacgg
     2401 cttaatcggc gagatggtag aacggtcatt ggcggaactg ccgagtggcg atccggtttc
     2461 attggccggg tggacccgta aactgaccct gcgcattatg ggccgcatta tcttcggtgt
     2521 cgatgatgat gaactgctga ctgcgttcac gcgttggtta gaccgtcgac tcggctctcg
     2581 ggtgcgcacg cttgcgcacc gttgcttcac tccaggcact acccgatttg atcctcatct
     2641 ggaccggctc cttctgcagc gagcacgtag tgcagaaccg ccggccctgg cggcttgttt
     2701 accgccagat gatttacgcg accagattgt tagcttgctg ttcgcaggtc acgaaacaac
     2761 agccagcgca actgcttgga ccgtctactg gatcgatcgc accccgggtc tggcagaaca
     2821 gttgttgact gatgaaacac tcctggacgc tgcaattact gagtctttgc gtttatcacc
     2881 tcctgcgaca ctggcaggca atcgtatgag tgatgacggc atggtcatca ccccgagcat
     2941 ctacctggcc caccgtcgtg cagcaacgta tgaaaatcca catacgtttg atccctcacg
     3001 taaaccgccg ccaccggggg ccttcttccc gttcggtgga ggcgttcgcc attgcccggg
     3061 gaaggacctc gcactgactg agattcgcat gatcgtgaca gctttgcttc gtcaccgtag
     3121 aatccgtctt aacaccccgc gatcttgtac cccgcaactg cgtggtgcag caatggcccc
     3181 gccgccaagc ctggcaatgg tggctacttg ccggcgttaa taaggatgag ctctacaaat
     3241 aagcagaggt ggttgtgttg cgaaaaaaaa aaaaaaacac cctaacgggt gttttttttt
     3301 ttttggtgtc cccacgtgtg gcgctggaga ccgtccaatg gcggcgcgcc atcgaatggt
     3361 gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga
     3421 atatgagccc gaaacgtcgt acccaggcag aacgtgcaat ggaaacccag ggtaaactga
     3481 ttgcagcagc actgggtgtt ctgcgtgaaa aaggttatgc aggttttcgt attgcagatg
     3541 ttccgggtgc agccggtgtt agccgtggtg cacagagcca tcattttccg accaaactgg
     3601 aactgctgct ggcaaccttt gaatggctgt atgagcagat taccgaacgt agccgtgcac
     3661 gtctggcaaa actgaaaccg gaagatgatg ttattcagca gatgctggat gatgcagcag
     3721 atttttttct ggatgatgat tttagcatcg gcctggatct gattgttgca gcagatcgtg
     3781 atccggcact gcgtgaaggt attctgcgta ccgttgaacg taatcgtttt gttgttgaag
     3841 atatgtggct gggtgtgctg gtgagccgtg gtctgagccg tgatgatgcc gaagatattc
     3901 tgtggctgat ttttaacagc gttcgtggtc tgacagttcg tagcctgtgg cagaaagata
     3961 aagaacgttt tgaacgtgtg cgtaatagca ccctggaaat tgcacgtgaa cgttatgcaa
     4021 aattcaaacg ttgataagga tcctaattgg taacgaatca gacaattgac ggctcgaggg
     4081 agtagcatag ggtttgcaga atccctgctt cgtccatttg acaggcacat tatgcatcga
     4141 tgataagctg tcaaacatga gcagatcctc tacgccggac gcatcgtggc cggcatcacc
     4201 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
     4261 gctcgccact tcgggctcat gagcaaatat tttatctgag gtgcttcctc gctc
//
