LOCUS       Exported                4553 bp ds-DNA     circular SYN 16-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Nov 17, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      22..858
                     /label=more stringent p15A
                     /label=more\stringent\p15A
     CDS             complement(930..1745)
                     /label=Kanamycin phosphotransferase
                     /label=Kanamycin\phosphotransferase
     terminator      1846..1891
                     /label=L3S3P21
                     /note="Geneious type: terminator"
     misc_feature    1898..1901
                     /label=4bp_scar5
     promoter        1922..2011
                     /label=pCymRC promoter
                     /label=pCymRC
     misc_feature    1922..1953
                     /label=CymO
                     /note="Geneious type: operator"
     promoter        1945..1950
                     /label=-35 promoter
                     /label=-35
     promoter        1968..1973
                     /label=-10 promoter
                     /label=-10
     misc_feature    1980..2011
                     /label=CymO
                     /note="Geneious type: operator"
     misc_feature    2013..2086
                     /label=RiboJ53
                     /note="Geneious type: riboj"
     primer_bind     complement(2052..2086)
                     /label=GML_amplify_Cym
     RBS             2087..2113
                     /note="TIR: 47502"
     CDS             2114..3463
                     /codon_start=1
                     /label=CYP065
                     /db_xref="UniProtKB/Swiss-Prot:A8M707"
                     /translation="MPAWKALPAAVRDTHRAFVDVGNWSDGDVVRVSLGVSRPYLVTNP
                     AHVQEVLHERAAIYPRGDDTALWRSVRKLVGDGILAEGDAWAASRRVLAPMFRPARINA
                     MVDTMADAIAGAIDDLHGAATAGTPIDVGRELSRIVCSAIMRVFFADRITVRDALRIMK
                     AQETIVTAMAPRILAPLVPWWIPMPGDRRFRAAVRSIDDILLPVLRQAQRQPDDGDDLL
                     SRLVHARADDGRALSEKRMRDDLVSMVAVTTETSTVVLTWLWPLLANHPDVANRLYDEI
                     DRVVGGGPVRGDHLAELTYTRMVLDELLRLYPAGWILPRRAATTDILGGVRINKGATVI
                     LSPYVTQRMTAWWGPTAEAFDPERFAAGREAADGRHRYAYYPFGVGMHRCLGEHLFNLE
                     AILIVATLLSRFRFALTDTSMPGVKVAASTRPARTVEVILKPVAPVPAR*"
     terminator      3505..3551
                     /label=L3S3P41
     misc_feature    3547
                     /label=C to G
     misc_feature    3561..3564
                     /label=4bp_scar6
     promoter        3580..3661
                     /label=Constitutive Promoter + RBS (DNA2.0 Vector)
                     /note="Geneious type: promoter"
     promoter        3598..3603
                     /label=-35 promoter
                     /label=-35
     misc_feature    3599
                     /label=C
                     /note="Geneious type: Editing History Replacement"
     promoter        3621..3626
                     /label=-10 promoter
                     /label=-10
     CDS             3662..4273
                     /label=CymR
     misc_feature    3959..3961
                     /label=E100D
                     /note="Geneious type: mutation"
     misc_feature    3961
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3989..3991
                     /label=S110
                     /note="Geneious type: mutation"
     misc_feature    3989
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4043..4045
                     /label=Q128L
                     /note="Geneious type: mutation"
     misc_feature    4044
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4172..4174
                     /label=A171
                     /note="Geneious type: mutation"
     misc_feature    4172
                     /label=G
                     /note="Geneious type: Editing History Replacement"
     terminator      4283..4537
                     /label=induction operon terminator region
ORIGIN
        1 actgactcgc tgcacgaggc agacctcagc gctagcggag tgtatactgg cttactatgt
       61 tggcactgat gagggtgtca gtgaagtgct tcatgtggca ggagaaaaaa ggctgcaccg
      121 gtgcgtcagc agaatatgtg atacaggata tattccgctt cctcgctcac tgactcgcta
      181 cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg
      241 gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc
      301 ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa
      361 acccgacagg actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc
      421 ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt
      481 ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa
      541 ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      601 gaaagacatg caaaagcacc actggcagca gccactggta attgatttag aggagttagt
      661 cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca agttttggtg actgcgctcc
      721 tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc
      781 ctgcaaggcg gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca
      841 agaagatcat cttattaagg ggtctgacgc tcagtggaac gaaaaatcaa tctaaagtat
      901 atatgagtaa acttggtctg acagttacct tagaaaaact catcgagcat caaatgaaac
      961 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     1021 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     1081 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     1141 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc
     1201 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     1261 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
     1321 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     1381 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg
     1441 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     1501 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     1561 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
     1621 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     1681 tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg ttgaatatgg
     1741 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat
     1801 atatttttat cttgtgcaat gtacatcaga gattttgaga cacaaccaat tattgaaggc
     1861 ctccctaacg gggggccttt ttttgtttct ggtctcccaa gcgcttaacg atcgttggct
     1921 gaacaaacag acaatctggt ctgtttgtat tatggaaaat ttttctgtat aatagattca
     1981 acaaacagac aatctggtct gtttgtatta tcagcggtca acgcatgtgc tttgcgttct
     2041 gatgagacag tgatgtcgaa accgcctcta caaataattt tgtttaccac ctcttctcat
     2101 aaggaggttt tttatgccag cctggaaagc cctgcctgcg gccgttcgtg atactcatcg
     2161 tgcctttgtg gatgtgggta actggtcaga cggtgatgtg gtgcgcgttt cccttggtgt
     2221 cagccgcccg tatctcgtga ccaatccggc gcacgtgcag gaagtgttac atgaacgggc
     2281 ggcaatctat ccgcgcggag atgataccgc actctggcgt agtgtacgca agttggtggg
     2341 tgatggcatc ttggctgaag gcgacgcgtg ggccgctagt agaagagtcc ttgcgccaat
     2401 gtttcgccct gcccgtatca acgccatggt agataccatg gcggatgcta tcgcaggcgc
     2461 tatagatgat ttgcacggcg cggcaacggc aggcacgccg attgacgttg gtcgcgaact
     2521 gagccgcatt gtgtgcagtg ccataatgcg tgtgttcttt gcagaccgta tcacggtacg
     2581 cgacgccctt cggatcatga aagcccagga aaccattgtg actgccatgg cgccgcgcat
     2641 tttagcacct ctggtaccgt ggtggattcc catgccgggg gatcgtagat tccgcgccgc
     2701 ggtgcgttca atcgatgaca ttctgctgcc cgtgcttaga caggcccagc gtcagcccga
     2761 cgatggcgac gatttattat cccgtttggt gcacgcgcgc gcagatgatg gtcgcgccct
     2821 gagcgaaaaa cgtatgcgcg acgatttggt gagcatggtc gcggttacta ccgaaacaag
     2881 caccgtggta ctgacatggt tgtggccttt gctggcgaac catcccgatg ttgcaaatcg
     2941 tctgtatgac gaaattgatc gcgttgttgg cggcggacca gtccgtggtg atcatctggc
     3001 ggaactgacg tatacccgca tggttctgga tgaattgtta cgactgtacc cggccggatg
     3061 gattctccct cgtcgggcag cgaccacgga tattctggga ggggtgcgta taaataaagg
     3121 tgccaccgta atcctgtcgc cctatgtaac acagcgtatg accgcatggt ggggtcccac
     3181 ggcagaagcc ttcgacccgg aaagattcgc ggcgggtcga gaagccgcgg atgggagaca
     3241 tcggtacgct tactatccct tcggcgtcgg catgcaccga tgtctggggg agcatctgtt
     3301 taacctggaa gctatcctca tcgttgccac gcttttgtca cgtttccgct ttgctctgac
     3361 ggatacaagc atgccgggag ttaaagtggc tgcctccacg cggccggctc gaaccgtaga
     3421 ggtgattctg aaacctgtcg cgccggttcc agcgcgttaa taaggatgag ctctacaaat
     3481 aagcagaggt ggttgtgttg cgaaaaaaaa aaaaaacacc ctaacgggtg tttttttttt
     3541 tttggtgtcc ccacgtgtgg cgctggagac cgtccaatgg cggcgcgcca tcgaatggtg
     3601 caaaaccttt cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa
     3661 tatgagcccg aaacgtcgta cccaggcaga acgtgcaatg gaaacccagg gtaaactgat
     3721 tgcagcagca ctgggtgttc tgcgtgaaaa aggttatgca ggttttcgta ttgcagatgt
     3781 tccgggtgca gccggtgtta gccgtggtgc acagagccat cattttccga ccaaactgga
     3841 actgctgctg gcaacctttg aatggctgta tgagcagatt accgaacgta gccgtgcacg
     3901 tctggcaaaa ctgaaaccgg aagatgatgt tattcagcag atgctggatg atgcagcaga
     3961 tttttttctg gatgatgatt ttagcatcgg cctggatctg attgttgcag cagatcgtga
     4021 tccggcactg cgtgaaggta ttctgcgtac cgttgaacgt aatcgttttg ttgttgaaga
     4081 tatgtggctg ggtgtgctgg tgagccgtgg tctgagccgt gatgatgccg aagatattct
     4141 gtggctgatt tttaacagcg ttcgtggtct gacagttcgt agcctgtggc agaaagataa
     4201 agaacgtttt gaacgtgtgc gtaatagcac cctggaaatt gcacgtgaac gttatgcaaa
     4261 attcaaacgt tgataaggat cctaattggt aacgaatcag acaattgacg gctcgaggga
     4321 gtagcatagg gtttgcagaa tccctgcttc gtccatttga caggcacatt atgcatcgat
     4381 gataagctgt caaacatgag cagatcctct acgccggacg catcgtggcc ggcatcaccg
     4441 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
     4501 ctcgccactt cgggctcatg agcaaatatt ttatctgagg tgcttcctcg ctc
//
