LOCUS       Exported                4424 bp ds-DNA     circular SYN 16-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Nov 17, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      22..858
                     /label=more stringent p15A
                     /label=more\stringent\p15A
     CDS             complement(930..1745)
                     /label=Kanamycin phosphotransferase
                     /label=Kanamycin\phosphotransferase
     terminator      1846..1891
                     /label=L3S3P21
                     /note="Geneious type: terminator"
     misc_feature    1898..1901
                     /label=4bp_scar5
     promoter        1922..2011
                     /label=pCymRC promoter
                     /label=pCymRC
     misc_feature    1922..1953
                     /label=CymO
                     /note="Geneious type: operator"
     promoter        1945..1950
                     /label=-35 promoter
                     /label=-35
     promoter        1968..1973
                     /label=-10 promoter
                     /label=-10
     misc_feature    1980..2011
                     /label=CymO
                     /note="Geneious type: operator"
     misc_feature    2013..2086
                     /label=RiboJ53
                     /note="Geneious type: riboj"
     primer_bind     complement(2052..2086)
                     /label=GML_amplify_Cym
     RBS             2087..2113
                     /note="TIR: 45631"
     CDS             2114..3334
                     /codon_start=1
                     /product="CYP068"
                     /label=CYP068
                     /db_xref="UniProtKB/Swiss-Prot:P18326"
                     /translation="MTDTATTPQTTDAPAFPSNRSCPYQLPDGYAQLRDTPGPLHRVTL
                     YDGRQAWVVTKHEAARKLLGDPRLSSNRTDDNFPATSPRFEAVRESPQAFIGLDPPEHG
                     TRRRMTISEFTVKRIKGMRPEVEEVVHGFLDEMLAAGPTADLVSQFALPVPSMVICRLL
                     GVPYADHEFFQDASKRLVQSTDAQSALTARNDLAGYLDGLITQFQTEPGAGLVGALVAD
                     QLANGEIDREELISTAMLLLIAGHETTASMTSLSVITLLDHPEQYAALRADRSLVPGAV
                     EELLRYLAIADIAGGRVATADIEVEGHLIRAGEGVIVVNSIANRDGTVYEDPDALDIHR
                     SARHHLAFGFGVHQCLGQNLARLELEVILNALMDRVPTLRLAVPVEQLVLRPGTTIQGV
                     NELPVTW"
     terminator      3376..3422
                     /label=L3S3P41
     misc_feature    3418
                     /label=C to G
     misc_feature    3432..3435
                     /label=4bp_scar6
     promoter        3451..3532
                     /label=Constitutive Promoter + RBS (DNA2.0 Vector)
                     /note="Geneious type: promoter"
     promoter        3469..3474
                     /label=-35 promoter
                     /label=-35
     misc_feature    3470
                     /label=C
                     /note="Geneious type: Editing History Replacement"
     promoter        3492..3497
                     /label=-10 promoter
                     /label=-10
     CDS             3533..4144
                     /label=CymR
     misc_feature    3830..3832
                     /label=E100D
                     /note="Geneious type: mutation"
     misc_feature    3832
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3860..3862
                     /label=S110
                     /note="Geneious type: mutation"
     misc_feature    3860
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3914..3916
                     /label=Q128L
                     /note="Geneious type: mutation"
     misc_feature    3915
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4043..4045
                     /label=A171
                     /note="Geneious type: mutation"
     misc_feature    4043
                     /label=G
                     /note="Geneious type: Editing History Replacement"
     terminator      4154..4408
                     /label=induction operon terminator region
ORIGIN
        1 actgactcgc tgcacgaggc agacctcagc gctagcggag tgtatactgg cttactatgt
       61 tggcactgat gagggtgtca gtgaagtgct tcatgtggca ggagaaaaaa ggctgcaccg
      121 gtgcgtcagc agaatatgtg atacaggata tattccgctt cctcgctcac tgactcgcta
      181 cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg
      241 gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc
      301 ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa
      361 acccgacagg actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc
      421 ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt
      481 ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa
      541 ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      601 gaaagacatg caaaagcacc actggcagca gccactggta attgatttag aggagttagt
      661 cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca agttttggtg actgcgctcc
      721 tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc
      781 ctgcaaggcg gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca
      841 agaagatcat cttattaagg ggtctgacgc tcagtggaac gaaaaatcaa tctaaagtat
      901 atatgagtaa acttggtctg acagttacct tagaaaaact catcgagcat caaatgaaac
      961 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     1021 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     1081 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     1141 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc
     1201 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     1261 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
     1321 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     1381 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg
     1441 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     1501 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     1561 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
     1621 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     1681 tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg ttgaatatgg
     1741 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat
     1801 atatttttat cttgtgcaat gtacatcaga gattttgaga cacaaccaat tattgaaggc
     1861 ctccctaacg gggggccttt ttttgtttct ggtctcccaa gcgcttaacg atcgttggct
     1921 gaacaaacag acaatctggt ctgtttgtat tatggaaaat ttttctgtat aatagattca
     1981 acaaacagac aatctggtct gtttgtatta tcagcggtca acgcatgtgc tttgcgttct
     2041 gatgagacag tgatgtcgaa accgcctcta caaataattt tgtttagagc aacatatccc
     2101 taggagaatt aaaatgacgg ataccgccac cacaccccag accacagatg ccccggcctt
     2161 ccctagcaac cgttcctgcc cgtaccaact cccggatggc tatgcacaac tccgtgatac
     2221 accgggcccg ttacaccgcg tgacattgta cgatggccgt caagcgtggg tggtgaccaa
     2281 gcatgaagcc gcacgtaaac tgcttggcga cccacgttta tcgtcaaatc gtacggacga
     2341 caatttcccg gcaacatccc cgcgttttga agcagtgcgt gagagcccac aggcctttat
     2401 cggcctggac ccgcccgaac atggtacgcg ccgtcggatg acgattagtg agttcacggt
     2461 taaacgcatt aagggcatgc gtccggaagt cgaagaggtg gttcacggtt tcctggatga
     2521 aatgctggcc gctggcccaa ctgcagactt agtcagtcaa tttgcgttgc cggttccatc
     2581 gatggtgatt tgccgcctct taggcgttcc gtatgcggac catgaattct tccaggatgc
     2641 gagtaaacgc ttggtgcaaa gtaccgacgc acagtccgcc ttgaccgccc gcaacgatct
     2701 ggccggttat ttggacggac tgattaccca gtttcaaacg gagccaggcg cgggattggt
     2761 tggcgccctt gtcgctgacc agctggcaaa tggtgaaatt gaccgagagg aattaatcag
     2821 caccgccatg ctgctgctga tcgcggggca cgaaaccacc gcctctatga cgtcattatc
     2881 ggtaattacg ctgctggatc atcctgaaca gtacgcggcc ctgcgtgcgg atcgttcgct
     2941 cgttcctggt gcagtggagg aacttctccg ctacttagcg attgccgaca ttgccggcgg
     3001 ccgtgttgcg acagccgata tcgaagtgga gggacatctg atacgtgcgg gtgaaggggt
     3061 gattgtggtg aacagcattg cgaaccgcga tggcaccgtt tatgaagatc cggatgcgct
     3121 ggatatacat cgtagtgcac ggcatcactt agcgttcggt tttggcgttc accagtgctt
     3181 agggcagaat ttggcccgct tagagttgga ggtcatcctt aacgccctga tggatcgggt
     3241 accgaccctg cgcttagccg ttcctgttga acagctggta ctccgtccgg gcaccaccat
     3301 tcaaggtgtt aacgaattgc cggtcacttg gtgaggatga gctctacaaa taagcagagg
     3361 tggttgtgtt gcgaaaaaaa aaaaaaacac cctaacgggt gttttttttt ttttggtgtc
     3421 cccacgtgtg gcgctggaga ccgtccaatg gcggcgcgcc atcgaatggt gcaaaacctt
     3481 tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga atatgagccc
     3541 gaaacgtcgt acccaggcag aacgtgcaat ggaaacccag ggtaaactga ttgcagcagc
     3601 actgggtgtt ctgcgtgaaa aaggttatgc aggttttcgt attgcagatg ttccgggtgc
     3661 agccggtgtt agccgtggtg cacagagcca tcattttccg accaaactgg aactgctgct
     3721 ggcaaccttt gaatggctgt atgagcagat taccgaacgt agccgtgcac gtctggcaaa
     3781 actgaaaccg gaagatgatg ttattcagca gatgctggat gatgcagcag atttttttct
     3841 ggatgatgat tttagcatcg gcctggatct gattgttgca gcagatcgtg atccggcact
     3901 gcgtgaaggt attctgcgta ccgttgaacg taatcgtttt gttgttgaag atatgtggct
     3961 gggtgtgctg gtgagccgtg gtctgagccg tgatgatgcc gaagatattc tgtggctgat
     4021 ttttaacagc gttcgtggtc tgacagttcg tagcctgtgg cagaaagata aagaacgttt
     4081 tgaacgtgtg cgtaatagca ccctggaaat tgcacgtgaa cgttatgcaa aattcaaacg
     4141 ttgataagga tcctaattgg taacgaatca gacaattgac ggctcgaggg agtagcatag
     4201 ggtttgcaga atccctgctt cgtccatttg acaggcacat tatgcatcga tgataagctg
     4261 tcaaacatga gcagatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag
     4321 gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact
     4381 tcgggctcat gagcaaatat tttatctgag gtgcttcctc gctc
//
