LOCUS       Exported                4594 bp ds-DNA     circular SYN 16-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Nov 17, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      22..858
                     /label=more stringent p15A
                     /label=more\stringent\p15A
     CDS             complement(930..1745)
                     /label=Kanamycin phosphotransferase
                     /label=Kanamycin\phosphotransferase
     terminator      1846..1891
                     /label=L3S3P21
                     /note="Geneious type: terminator"
     misc_feature    1898..1901
                     /label=4bp_scar5
     promoter        1922..2011
                     /label=pCymRC promoter
                     /label=pCymRC
     misc_feature    1922..1953
                     /label=CymO
                     /note="Geneious type: operator"
     promoter        1945..1950
                     /label=-35 promoter
                     /label=-35
     promoter        1968..1973
                     /label=-10 promoter
                     /label=-10
     misc_feature    1980..2011
                     /label=CymO
                     /note="Geneious type: operator"
     misc_feature    2013..2086
                     /label=RiboJ53
                     /note="Geneious type: riboj"
     primer_bind     complement(2052..2086)
                     /label=GML_amplify_Cym
     RBS             2087..2112
                     /note="TIR: 49878"
     CDS             2113..3504
                     /codon_start=1
                     /product="CYP072"
                     /label=CYP072
                     /db_xref="UniProtKB/Swiss-Prot:Q5KSN6"
                     /translation="MPELPAIRRAPRRLPLAGHAVPLLVQPLEFLKSLPALGDMVRVRM
                     GPDDAYVPCDPALFRQILKDTRLYDKGGLFYDRGREAVGNGLVTSRWADHRRQRPLMQP
                     SFDHRHIGHYAELMADETEALMRSWRSGEVVDVDKAMARLTLRITTRSLFSVPADHTLV
                     AQVEKWLPILMDGFFRRMFVPARVLSLVPGKMNRQYPPPIAEMRKLTEEIIDEVRRKKD
                     EDPGLLAGLMNARDETTGAPLGTQEIFDQVLILLIAGSETTATALAFTFHLLGAHPEIG
                     ARLREEVDGALGGRTPRFEDLSGLTFTRQVLMESLRLYPPAWMFTRSTTTACELGGHAF
                     PEGTTFLLSPYILQHDPELFPRPERFDPDRWRPGAMSDVARRSVLPFGAGGRKCIGDQF
                     ALNEAMLAVAAIAGRWNLTPVGDRPIRPIARATLKTGPLPMRLQERPHEPVRNATTTDL
                     RDQR*"
     terminator      3546..3592
                     /label=L3S3P41
     misc_feature    3588
                     /label=C to G
     misc_feature    3602..3605
                     /label=4bp_scar6
     promoter        3621..3702
                     /label=Constitutive Promoter + RBS (DNA2.0 Vector)
                     /note="Geneious type: promoter"
     promoter        3639..3644
                     /label=-35 promoter
                     /label=-35
     misc_feature    3640
                     /label=C
                     /note="Geneious type: Editing History Replacement"
     promoter        3662..3667
                     /label=-10 promoter
                     /label=-10
     CDS             3703..4314
                     /label=CymR
     misc_feature    4000..4002
                     /label=E100D
                     /note="Geneious type: mutation"
     misc_feature    4002
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4030..4032
                     /label=S110
                     /note="Geneious type: mutation"
     misc_feature    4030
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4084..4086
                     /label=Q128L
                     /note="Geneious type: mutation"
     misc_feature    4085
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4213..4215
                     /label=A171
                     /note="Geneious type: mutation"
     misc_feature    4213
                     /label=G
                     /note="Geneious type: Editing History Replacement"
     terminator      4324..4578
                     /label=induction operon terminator region
ORIGIN
        1 actgactcgc tgcacgaggc agacctcagc gctagcggag tgtatactgg cttactatgt
       61 tggcactgat gagggtgtca gtgaagtgct tcatgtggca ggagaaaaaa ggctgcaccg
      121 gtgcgtcagc agaatatgtg atacaggata tattccgctt cctcgctcac tgactcgcta
      181 cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg
      241 gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc
      301 ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa
      361 acccgacagg actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc
      421 ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt
      481 ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa
      541 ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      601 gaaagacatg caaaagcacc actggcagca gccactggta attgatttag aggagttagt
      661 cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca agttttggtg actgcgctcc
      721 tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc
      781 ctgcaaggcg gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca
      841 agaagatcat cttattaagg ggtctgacgc tcagtggaac gaaaaatcaa tctaaagtat
      901 atatgagtaa acttggtctg acagttacct tagaaaaact catcgagcat caaatgaaac
      961 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     1021 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     1081 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     1141 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc
     1201 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     1261 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
     1321 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     1381 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg
     1441 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     1501 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     1561 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
     1621 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     1681 tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg ttgaatatgg
     1741 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat
     1801 atatttttat cttgtgcaat gtacatcaga gattttgaga cacaaccaat tattgaaggc
     1861 ctccctaacg gggggccttt ttttgtttct ggtctcccaa gcgcttaacg atcgttggct
     1921 gaacaaacag acaatctggt ctgtttgtat tatggaaaat ttttctgtat aatagattca
     1981 acaaacagac aatctggtct gtttgtatta tcagcggtca acgcatgtgc tttgcgttct
     2041 gatgagacag tgatgtcgaa accgcctcta caaataattt tgtttaggag gaagaccact
     2101 aaggaggaga taatgccaga actgccagca atccgccgtg cccctagacg tctgcctttg
     2161 gccgggcacg ctgttccatt actggtccaa ccgttggagt ttctcaaatc tctgccggcc
     2221 ttaggtgaca tggttcgtgt gcgtatggga ccggacgatg cgtatgttcc ttgtgatcct
     2281 gctctgttcc gtcaaattct caaagacacc agactctatg acaaaggtgg cctgttttat
     2341 gatcgcgggc gtgaggcagt cgggaatggt ctggttacct cgcggtgggc tgaccatcgc
     2401 cgccagcgtc ccctgatgca accgtccttc gaccaccgtc atatcgggca ttatgcagaa
     2461 ctgatggcag atgaaaccga agcattaatg cggtcctggc gtagtggcga agtggtggat
     2521 gtggataaag caatggcccg gctcacgtta cgcattacta cccgtagtct gttttctgtg
     2581 cccgccgatc atactctggt cgcgcaagtc gaaaagtggc tgcccatcct gatggacgga
     2641 ttcttccgcc gcatgtttgt cccagcgcgt gtattatccc tggtccctgg taaaatgaac
     2701 cgtcagtatc cgcctccgat tgccgaaatg cgtaaattga ccgaggagat tatcgatgaa
     2761 gtacgtcgta agaaggacga agatccgggc ctgttggcgg gactgatgaa tgcacgcgat
     2821 gaaacgaccg gcgcgccgtt gggtactcag gagatttttg accaagtcct cattttgctg
     2881 atcgcaggta gcgaaacgac cgcgacggcc ctggccttta cctttcacct cctgggggcc
     2941 catccagaaa ttggggcacg tttacgtgaa gaagttgacg gagcattggg tggccgcacc
     3001 cctcggtttg aggatctgtc tggtctgacc tttacacgtc aggttctgat ggaaagcctc
     3061 cgtctgtacc caccagcatg gatgttcacc cgttctacca ccacggcatg cgagctgggc
     3121 ggccacgctt ttccggaggg caccacgttt ttgctcagtc catatatcct gcagcatgat
     3181 ccggaactct ttccgcgccc ggaacgtttc gatcctgatc gctggcgccc aggtgcgatg
     3241 tctgacgtcg cccgacgtag tgttttgccg ttcggggcgg gtgggcgcaa atgtattggt
     3301 gatcagtttg cgctcaacga agccatgtta gcagtggccg cgatcgctgg ccgctggaat
     3361 ctcactccgg tgggagatag acccattcgc cctattgctc gcgccacatt gaaaactggt
     3421 ccactgccaa tgcgactgca ggaacgcccg cacgaaccgg ttcgtaatgc gactaccact
     3481 gacctccgcg accagcggta ataaggatga gctctacaaa taagcagagg tggttgtgtt
     3541 gcgaaaaaaa aaaaaaacac cctaacgggt gttttttttt ttttggtgtc cccacgtgtg
     3601 gcgctggaga ccgtccaatg gcggcgcgcc atcgaatggt gcaaaacctt tcgcggtatg
     3661 gcatgatagc gcccggaaga gagtcaattc agggtggtga atatgagccc gaaacgtcgt
     3721 acccaggcag aacgtgcaat ggaaacccag ggtaaactga ttgcagcagc actgggtgtt
     3781 ctgcgtgaaa aaggttatgc aggttttcgt attgcagatg ttccgggtgc agccggtgtt
     3841 agccgtggtg cacagagcca tcattttccg accaaactgg aactgctgct ggcaaccttt
     3901 gaatggctgt atgagcagat taccgaacgt agccgtgcac gtctggcaaa actgaaaccg
     3961 gaagatgatg ttattcagca gatgctggat gatgcagcag atttttttct ggatgatgat
     4021 tttagcatcg gcctggatct gattgttgca gcagatcgtg atccggcact gcgtgaaggt
     4081 attctgcgta ccgttgaacg taatcgtttt gttgttgaag atatgtggct gggtgtgctg
     4141 gtgagccgtg gtctgagccg tgatgatgcc gaagatattc tgtggctgat ttttaacagc
     4201 gttcgtggtc tgacagttcg tagcctgtgg cagaaagata aagaacgttt tgaacgtgtg
     4261 cgtaatagca ccctggaaat tgcacgtgaa cgttatgcaa aattcaaacg ttgataagga
     4321 tcctaattgg taacgaatca gacaattgac ggctcgaggg agtagcatag ggtttgcaga
     4381 atccctgctt cgtccatttg acaggcacat tatgcatcga tgataagctg tcaaacatga
     4441 gcagatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc
     4501 tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat
     4561 gagcaaatat tttatctgag gtgcttcctc gctc
//
