LOCUS       292_CAGGS_TetR_d        8362 bp DNA     circular SYN 22-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 22, 2023 from SnapGene Viewer 7.0.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            327..986
                     /label=AmpR
     misc_feature    1084..1766
                     /label=ColE1_origin
                     /label=nonstandard type: Origin
     misc_feature    2106..2128
                     /label=LacO
                     /label=nonstandard type: binding_site
     misc_feature    2223..2548
                     /label=ePiggyBac_5'ITR
                     /label=nonstandard type: ITR-PB
     promoter        2569..4902
                     /label=CAGGS
     misc_feature    3633..4310
                     /note="/feature_type=Duplication"
     misc_feature    4934..5557
                     /label=TetR
     misc_feature    5567..5632
                     /label=ICP22NLS
     misc_feature    5645..7075
                     /label=tdTomato
                     /label=dTomato
                     /note="/feature_type=fluorescent protein"
     misc_feature    7107..7116
                     /label=attL
                     /note="/feature_type=recombination site"
     misc_feature    7137..7145
                     /label=AU rich element
                     /note="/feature_type=destabilization element"
     misc_feature    7151..7159
                     /label=AU rich element
                     /note="/feature_type=destabilization element"
     misc_feature    7165..7173
                     /label=AU rich element
                     /note="/feature_type=destabilization element"
     misc_feature    7179..7187
                     /label=AU rich element
                     /note="/feature_type=destabilization element"
     misc_feature    7200..7437
                     /label=SV40 poly(A)
                     /note="/feature_type=polyadenylation signal"
     misc_feature    7443..7696
                     /label=ePiggyBac_3'ITR
                     /label=nonstandard type: ITR-PB
     gene            7835..7903
                     /label=LacZ
     rep_origin      7922..8228
                     /label=F1_ori
                     /label=F1_oru
     misc_feature    join(8261^8266,8263^8264)
                     /label=PsiI
                     /label=nonstandard type: Restriction_sit
                     /note="Name: PsiI"
                     /note="Pattern: ttataa"
                     /note="Number_of_matches: 3"
                     /note="cut_0_on_positive_strand: 6491^6492"
                     /note="cut_0_on_negative_strand: 6491^6492"
                     /note="inhibited_by: "
                     /note="site_type: other"
                     /note="restriction site"
     misc_feature    join(8261^8266,8263^8264)
                     /label=PsiI
                     /label=nonstandard type: Restriction_sit
                     /note="Name: PsiI"
                     /note="Pattern: ttataa"
                     /note="Number_of_matches: 3"
                     /note="cut_0_on_positive_strand: 6491^6492"
                     /note="cut_0_on_negative_strand: 6491^6492"
                     /note="inhibited_by: "
                     /note="site_type: other"
                     /note="restriction site"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggggcgc
     2221 gccgcagcta gattaaccct agaaagatag tctgcgtaaa attgacgcat gcattcttga
     2281 aatattgctc tctctttcta aatagcgcga atccgtcgct gtgcatttag gacatctcag
     2341 tcgccgcttg gagctcccgt gaggcgtgct tgtcaatgcg gtaagtgtca ctgattttga
     2401 actataacaa ccgcgtgagt caaaatgacg catgattatc ttttacgtga cttttaagat
     2461 ttaactcata cgataattat attgttattt cgtgttctac ttacgtgata acttattata
     2521 tatatatttt cttgttatag atatccttct cgacattgat tattgactag agatctatca
     2581 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     2641 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     2701 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     2761 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     2821 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     2881 cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt
     2941 tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt
     3001 tttaattatt ttgtgcagcg atgggggcga ggggcggggc ggggcgaggc ggagaggtgc
     3061 ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg
     3121 gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg
     3181 ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt
     3241 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt
     3301 ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg
     3361 ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
     3421 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
     3481 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
     3541 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
     3601 tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca
     3661 cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg
     3721 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg
     3781 ctcgggggag ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
     3841 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
     3901 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga
     3961 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc
     4021 gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg
     4081 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctgt gagcgctgcg
     4141 ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg
     4201 gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     4261 tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc
     4321 ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg
     4381 cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc
     4441 cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg cccccggagc gccggcggct
     4501 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     4561 gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct
     4621 ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc
     4681 ttcgtgcgtc gccgcgccgc cgtccccctt ctccctctcc agcctcgggg ctgtccgcgg
     4741 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     4801 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     4861 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcct cgagaattct
     4921 cacgcgcgcc accatgatgt ccagattaga taaaagtaaa gtgattaaca gcgcattaga
     4981 gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc agaagctagg
     5041 tgtagagcag cctacattgt attggcatgt aaaaaataag cgggctttgc tcgacgcctt
     5101 agccattgag atgttagata ggcaccatac tcacttttgc cctttagaag gggaaagctg
     5161 gcaagatttt ttacgtaata acgctaaaag ttttagatgt gctttactaa gtcatcgcga
     5221 tggagcaaaa gtacatttag gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa
     5281 tcaattagcc tttttatgcc aacaaggttt ttcactagag aatgcattat atgcactcag
     5341 cgctgtgggg cattttactt taggttgcgt attggaagat caagagcatc aagtcgctaa
     5401 agaagaaagg gaaacaccta ctactgatag tatgccgcca ttattacgac aagctatcga
     5461 attatttgat caccaaggtg cagagccagc cttcttattc ggccttgaat tgatcatatg
     5521 cggattagaa aaacaactta aatgtgaaag tgggtccgcg gatctaaagg cacgtcgtcc
     5581 agcactaaga tcaccaccac taggaactcg taagcgaaag cgtccttctc gggatcacgc
     5641 caccatggtg agcaagggcg aggaggtcat caaagagttc atgcgcttca aggtgcgcat
     5701 ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     5761 cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg
     5821 ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc accccgccga
     5881 catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     5941 cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg acggcacgct
     6001 gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg taatgcagaa
     6061 gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa
     6121 gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa
     6181 gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg tggacaccaa
     6241 gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga
     6301 gggccgccac cacctgttcc tggggcatgg caccggcagc accggcagcg gcagctccgg
     6361 caccgcctcc tccgaggaca acaacatggc cgtcatcaaa gagttcatgc gcttcaaggt
     6421 gcgcatggag ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg
     6481 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt
     6541 cgcctgggac atcctgtccc cccagttcat gtacggctcc aaggcgtacg tgaagcaccc
     6601 cgccgacatc cccgattaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     6661 gatgaacttc gaggacggcg gtctggtgac cgtgacccag gactcctccc tgcaggacgg
     6721 cacgctgatc tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat
     6781 gcagaagaag accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt
     6841 gctgaagggc gagatccacc aggccctgaa gctgaaggac ggcggccact acctggtgga
     6901 gttcaagacc atctacatgg ccaagaagcc cgtgcaactg cccggctact actacgtgga
     6961 caccaagctg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg
     7021 ctccgagggc cgccaccacc tgttcctgta cggcatggac gagctgtaca agtaagtgcc
     7081 taggtggccg cactcgagat atctagaccc agctttcttg tacaaagtgg tgatccttat
     7141 ttattgatcc ttatttattg tagcttattt attgctactt atttattgat ctgcggccgc
     7201 gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     7261 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     7321 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     7381 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggaa
     7441 ttcgataaaa gttttgttac tttatagaag aaattttgag tttttgtttt tttttaataa
     7501 ataaataaac ataaataaat tgtttgttga atttattatt agtatgtaag tgtaaatata
     7561 ataaaactta atatctattc aaattaataa ataaacctcg atatacagac cgataaaaca
     7621 catgcgtcaa ttttacgcat gattatcttt aacgtacgtc acaatatgat tatctttcta
     7681 gggttaatct agctgcggcg cgccggtacc caattcgccc tatagtgagt cgtattacgc
     7741 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     7801 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     7861 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     7921 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     7981 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     8041 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     8101 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     8161 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     8221 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     8281 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     8341 aatattaacg cttacaattt ag
//
