LOCUS       polio_3ABC_WT_03              4479 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             3449..4309
                     /note="pLannotate"
                     /label="AmpR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     rep_origin      2555..3010
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     misc_feature    complement(1859..2358)
                     /note="pLannotate"
                     /label="IRES"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="90.57971014492753"
                     /fragment="True"
                     /other="misc feature"
     promoter        3344..3448
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    complement(3011..3112)
                     /note="pLannotate"
                     /label="bom"
                     /database="addgene"
                     /identity="98.039"
                     /match_length="73.38129496402878"
                     /fragment="True"
                     /other="misc feature"
     terminator      complement(789..836)
                     /note="pLannotate"
                     /label="T7 terminator"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     CDS             complement(1827..1853)
                     /note="pLannotate"
                     /label="HA"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(2373..2391)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             2394..2554
                     /note="pLannotate"
                     /label="lacZ"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="5.235772357723578"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(3214..3249)
                     /note="pLannotate"
                     /label="rop"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="19.047619047619047"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcccgga tatagttcct
      781 cctttcagca aaaaacccct caagacccgt ttagaggccc caaggggtta tgctagttat
      841 tgctcagcgg actagtgttt aaactttttt tttttttttt tttttttttt ttcagtcaga
      901 tctcagtggt ggtggtggtg gtgctcgaga ctctgagtga agtatgatcg cttcagggcc
      961 gctgcaaacc cgtgtgaacc gttcccacca acatgcatcc cgatgacttt cccagtacat
     1021 gtgatgactc caccacactg tcctgctctg gttggaaagt tgtacattag agtacgagca
     1081 gtttggcgcc caccgagatt tagatatccc tgttcagtca cagcaccgac aggaacatac
     1141 atattggggt acttgctagt gttcacgatc aagactccat catttgtctc agtgatttga
     1201 gtaggtatat gtggtctaat gtctctgaac ttttcatttc tctttagagt gattatagtg
     1261 atttcaagat tggttcctgc ttgatcttcg agcgctttgg catccaagat ctccacttct
     1321 ttgccatcga tcacaatgct ttcaccaggt gaagcgtggg ttggtaaaat agccacgttg
     1381 tcgtggactc ctaacatagt gaactctccc ttgctagtag ttgctgtaac aatgtttctt
     1441 ttagccatag ccactgcgta atcgaaccct ggtccttgta cctttgctgt ccgaatggtg
     1501 ggcacgttgg gttttttgtt tggtaaacca gtgtatgctc cctggtgtcc agcaaacagt
     1561 ttatacatga catagacaac tccagccact gcggcgaagg ttgtcaccgc ttgtagaatt
     1621 gtcattgccc tgttgatgtt cctttctgtt tgaacctggc tggtgatgtt gactatccaa
     1681 cccttcttct cacagtaatc tctcacctcc tgggagtcaa ctgcttggag caagtcattg
     1741 atacattcag gagggggact cgtcttgatg tcaattttca agtctttata ctggagtggt
     1801 ccggatccca tatgggtggt ggccagggcg tagtcgggca cgtcgtaggg gtaggccata
     1861 ttatcatcgt gtttttcaaa ggaaaaccac gtccccgtgg ttcggggggc ctagacgttt
     1921 tttaacctcg actaaacaca tgtaaagcat gtgcaccgag gccccagatc agatcccata
     1981 caatggggta ccttctgggc atccttcagc cccttgttga atacgcttga ggtgagccat
     2041 ttgactcttt ccacaactat ccaactcaca acgtggcact ggggttgtgc cgcctttgca
     2101 ggtgtatctt atacacgtgg cttttggccg cagaggcacc tgtcgccagg tggggggttc
     2161 cgctgcctgc aaagggtcgc tacagacgtt gtttgtcttc aagaagcttc cagaggaact
     2221 gcttccttca cgacattcaa cagaccttgc attcctttgg cgagagggga aagaccccta
     2281 ggaatgctcg tcaagaagac agggccaggt ttccgggccc tcacattgcc aaaagacggc
     2341 aatatggtgg aaaataaccg gaattaattc gccctatagt gagtcgtatt acaattcact
     2401 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     2461 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     2521 ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg cgcattaagc
     2581 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     2641 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     2701 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     2761 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     2821 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     2881 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     2941 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     3001 cttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     3061 tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     3121 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     3181 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     3241 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     3301 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     3361 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     3421 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     3481 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     3541 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     3601 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     3661 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     3721 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     3781 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     3841 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     3901 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     3961 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     4021 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     4081 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     4141 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     4201 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     4261 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     4321 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     4381 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     4441 ctgagcgtca gaccccgtag aaaagatcaa aggatcttc
//
