LOCUS       polio_VP12A_WT_01              4969 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             3939..4799
                     /note="pLannotate"
                     /label="AmpR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     rep_origin      3045..3500
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     misc_feature    complement(2349..2848)
                     /note="pLannotate"
                     /label="IRES"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="90.57971014492753"
                     /fragment="True"
                     /other="misc feature"
     promoter        3834..3938
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    complement(3501..3602)
                     /note="pLannotate"
                     /label="bom"
                     /database="addgene"
                     /identity="98.039"
                     /match_length="73.38129496402878"
                     /fragment="True"
                     /other="misc feature"
     terminator      complement(789..836)
                     /note="pLannotate"
                     /label="T7 terminator"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     CDS             complement(2317..2343)
                     /note="pLannotate"
                     /label="HA"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(2863..2881)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             2884..3044
                     /note="pLannotate"
                     /label="lacZ"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="5.235772357723578"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(3704..3739)
                     /note="pLannotate"
                     /label="rop"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="19.047619047619047"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcccgga tatagttcct
      781 cctttcagca aaaaacccct caagacccgt ttagaggccc caaggggtta tgctagttat
      841 tgctcagcgg actagtgttt aaactttttt tttttttttt tttttttttt tttcagtcag
      901 atctcagtgg tggtggtggt ggtgctcgag ttgttccatg gcttcttctt cgtaggcata
      961 caagtctcta atgtctgaaa atgcaaccaa cccttcgcca ccagcagtaa tgatccctat
     1021 caccccgtgg tgacatctga gtatgccacc acaatcccct ggagatgcga atccatggcc
     1081 aatgagcata tgggactggt acctagctgg gtaatagtta ttagcctcca tgtactggaa
     1141 cgttgggcca acgaaggata ctgggtagta tttccttcta gactcgcagt agtacacccc
     1201 tgcgttgcaa ttgcaccttg cgattgaatc ggtgccctgg gctcttgatt ctgtgactaa
     1261 gaggtctcta ctccacatga cgttcactgc gttttgcaaa tcatcctgag tggccaagtg
     1321 gtagttgcaa attttgtaac ctgcagtgta caccgctttg ttttggtgtc cgaatccata
     1381 tgtggtcaga tccttggtgg agaggggtgt aagcgtacca tccttgtaat ccactccagg
     1441 gccgtagtac gccactgccc tcggtggacg cgggcaccag actctgatgt gtttgggttt
     1501 tagatacact ctgattttgg aggtgacctt ggtcgggttg tgatcattga ctactctaac
     1561 agccaaaata ccgaagtcat ttagagatgc tgcaccataa agggagtcac ctagtgctgc
     1621 cgactggtcc ttcagtggta ctttggaaaa accgtcgtaa aagtgtgaat aggcgttcga
     1681 aataccaaca tacggtaccg agatccgggc tggagctgtt ccgtaggtgt aaaagattga
     1741 tggatttgat gaggtttgcc atgtgtagtc gtcccatttc tcgggcactg gagcgcctgg
     1801 tggtacgtac ataatttggt acacttgatt taaggcatgc ccattgttag tctcagtgaa
     1861 atttgcagta accacaaagg taagttccat atcaaatcta gaataggtga agaactccaa
     1921 tttcctccgt aactggacag tatctttata agtgatcttc cacactgcaa atagcttagc
     1981 ataatcaggg acatcataag gataagctgg gttatccacg gtcataatgg tcacgcatgc
     2041 accccgcgcg aagaaagact ctatgctaga ctctgacctt gacctatgtt gtacaacatg
     2101 tctggtttgc actgtatcag aagggactag tggatttgtg gccccagttt ccactgcggt
     2161 gagtgccgga atttccttgg agtgtgttgg tccactggct tcagtgtttg ggagagcgtc
     2221 tctagatgtt gccgccccca ccgtttcacg gactgtgttg tcaatcatgc tttcaagcat
     2281 ctgacctaac ccggatccca tatgggtggt ggccagggcg tagtcgggca cgtcgtaggg
     2341 gtaggccata ttatcatcgt gtttttcaaa ggaaaaccac gtccccgtgg ttcggggggc
     2401 ctagacgttt tttaacctcg actaaacaca tgtaaagcat gtgcaccgag gccccagatc
     2461 agatcccata caatggggta ccttctgggc atccttcagc cccttgttga atacgcttga
     2521 ggtgagccat ttgactcttt ccacaactat ccaactcaca acgtggcact ggggttgtgc
     2581 cgcctttgca ggtgtatctt atacacgtgg cttttggccg cagaggcacc tgtcgccagg
     2641 tggggggttc cgctgcctgc aaagggtcgc tacagacgtt gtttgtcttc aagaagcttc
     2701 cagaggaact gcttccttca cgacattcaa cagaccttgc attcctttgg cgagagggga
     2761 aagaccccta ggaatgctcg tcaagaagac agggccaggt ttccgggccc tcacattgcc
     2821 aaaagacggc aatatggtgg aaaataaccg gaattaattc gccctatagt gagtcgtatt
     2881 acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     2941 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     3001 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg
     3061 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     3121 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     3181 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     3241 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     3301 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     3361 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     3421 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     3481 aatattaacg cttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     3541 tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     3601 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     3661 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     3721 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     3781 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     3841 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     3901 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     3961 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     4021 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     4081 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     4141 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     4201 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     4261 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     4321 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     4381 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     4441 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     4501 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     4561 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     4621 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     4681 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     4741 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     4801 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     4861 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     4921 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttc
//
