LOCUS       pMT646_ORFeus_OR       15135 bp DNA     circular SYN 28-JUN-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15135)
  AUTHORS   Marty Taylor
  TITLE     Direct Submission
  JOURNAL   Exported Nov 27, 2023 from SnapGene 7.0.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15135
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..588
                     /label=CMV Promoter
                     /note="color: #993366; direction: RIGHT"
     misc_feature    619..647
                     /label=MCS
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     661..687
                     /label=MTp905_ORFeus-Fwd-Lenti2
                     /note="color: black; sequence: 
                     gtgaGCCCGGGCTTAATTAAGCCACCATGGGCAAGAAG; added: 2023-02-23"
     primer_bind     662..687
                     /label=MTp901_ORFeus-Fwd-C
                     /note="color: black; sequence: 
                     gtttgccgccagaacacaggTTAATTAAGCCACCATGGGCAAGAAG; added: 
                     2023-02-23"
     primer_bind     670..689
                     /label=MTp954_ORFeus-LentiFwd
                     /note="color: black; sequence: 
                     atggatcctgacgcgtGCCACCATGGGCAAGAAGCA; added: 2023-04-19"
     primer_bind     670..688
                     /label=MTp962_ORFeus-Lenti
                     /note="color: black; sequence: 
                     cacttcctaccctcgtaaagGCCCGGGCGCCACCATGGGCAAGAAGC; added: 
                     2023-06-21"
     primer_bind     670..688
                     /label=MTp964_ORFeus-LentiFwd-Long
                     /note="color: black; sequence: 
                     ctttccgtaccacttcctaccctcgtaaagGCCCGGGCGCCACCATGGGCAAGAAGC; 
                     added: 2023-06-28"
     regulatory      670..679
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             676..1692
                     /codon_start=1
                     /label=ORF1
                     /note="color: #993366"
                     /translation="MGKKQNRKTGNSKTQSASPPPKERSSSPATEQSWMENDFDELREE
                     GFRRSNYSELREDIQTKGKEVENFEKNLEECITRITNTEKCLKELMELKTKARELREEC
                     RSLRSRCDQLEERVSAMEDEMNEMKREGKFREKRIKRNEQSLQEIWDYVKRPNLRLIGV
                     PESDVENGTKLENTLQDIIQENFPNLARQANVQIQEIQRTPQRYSSRRATPRHIIVRFT
                     KVEMKEKMLRAAREKGRVTLKGKPIRLTADLSAETLQARREWGPIFNILKEKNFQPRIS
                     YPAKLSFISEGEIKYFIDKQMLRDFVTTRPALKELLKEALNMERNNRYQPLQNHAKM"
     primer_bind     complement(1671..1691)
                     /label=MTp907_ORFeus-ORF1-pcDNA-Rev
                     /note="color: black; sequence: 
                     GGTTGATTGGATCCTCACTACATCTTGGCGTGGTTCTGC; added: 2023-03-14"
     primer_bind     1711..1737
                     /label=MTp342_Inter-ORF Fwd
                     /note="color: black; sequence: AGAAACTGCATCAACTAATGAGCAAAA;
                     added: 2020-07-07"
     CDS             1756..5695
                     /codon_start=1
                     /label=Orf2-PPX-3xFlag
                     /note="color: #993366"
                     /translation="MTGSNSHITILTLNINGLNSAIKRHRLASWIKSQDPSVCCIQETH
                     LTCRDTHRLKIKGWRKIYQANGKQKKAGVAILVSDKTDFKPTKIKRDKEGHYIMVKGSI
                     QQEELTILNIYAPNTGAPRFIKQVLSDLQRDLDSHTLIMGDFNTPLSTLDRSTRQKVNK
                     DTQELNSALHQADLIDIYRTLHPKSTEYTFFSAPHHTYSKIDHIVGSKALLSKCKRTEI
                     ITNYLSDHSAIKLELRIKNLTQSRSTTWKLNNLLLNDYWVHNEMKAEIKMFFETNENKD
                     TTYQNLWDAFKAVCRGKFIALNAYKRKQERSKIDTLTSQLKELEKQEQTHSKASRRQEI
                     TKIRAELKEIETQKTLQKINESRSWFFERINKIDRPLARLIKKKREKNQIDTIKNDKGD
                     ITTDPTEIQTTIREYYKHLYANKLENLEEMDTFLDTYTLPRLNQEEVESLNRPITGSEI
                     VAIINSLPTKKSPGPDGFTAEFYQRYKEELVPFLLKLFQSIEKEGILPNSFYEASIILI
                     PKPGRDTTKKENFRPISLMNIDAKILNKILANRIQQHIKKLIHHDQVGFIPGMQGWFNI
                     RKSINVIQHINRAKDKNHMIISIDAEKAFDKIQQPFMLKTLNKLGIDGTYFKIIRAIYD
                     KPTANIILNGQKLEAFPLKTGTRQGCPLSPLLFNIVLEVLARAIRQEKEIKGIQLGKEE
                     VKLSLFADDMIVYLENPIVSAQNLLKLISNFSKVSGYKINVQKSQAFLYTNNRQTESQI
                     MGELPFTIASKRIKYLGIQLTRDVKDLFKENYKPLLKEIKEETNKWKNIPCSWVGRINI
                     VKMAILPKVIYRFNAIPIKLPMTFFTELEKTTLKFIWNQKRARIAKSILSQKNKAGGIT
                     LPDFKLYYKATVTKTAWYWYQNRDIDQWNRTEPSEIMPHIYNYLIFDKPEKNKQWGKDS
                     LFNKWCWENWLAICRKLKLDPFLTPYTKINSRWIKDLNVKPKTIKTLEENLGITIQDIG
                     VGKDFMSKTPKAMATKDKIDKWDLIKLKSFCTAKETTIRVNRQPTTWEKIFATYSSDKG
                     LISRIYNELKQIYKKKTNNPIKKWAKDMNRHFSKEDIYAAKKHMKKCSSSLAIREMQIK
                     TTMRYHLTPVRMAIIKKSGNNRCWRGCGEIGTLLHCWWDCKLVQPLWKSVWRFLRDLEL
                     EIPFDPAIPLLGIYPNEYKSCCYKDTCTRMFIAALFTIAKTWNQPKCPTMIDWIKKMWH
                     IYTMEYYAAIKNDEFISFVGTWMKLETIILSKLSQEQKTKHRIFSLIGGNGGGSGGGSL
                     EVLFQGPDYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    2188..2190
                     /label=D145
                     /note="color: #a6acb3"
     primer_bind     complement(2345..2364)
                     /label=MTp301_ORF2_500_rev
                     /note="color: black; sequence: CTTGCTGTAGGTGTGGTGGG; added:
                     2021-01-28"
     primer_bind     2345..2364
                     /label=MTp308_ORF2_500f_v2
                     /note="color: black; sequence: CCCACCACACCTACAGCAAG; added:
                     2021-01-28"
     misc_feature    2368..2370
                     /label=D205
                     /note="color: #a6acb3"
     misc_feature    2444..2447
                     /label=H230 Endo
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(2580..2601)
                     /label=MTp343_ORF2-BstBI-Rev
                     /note="color: black; sequence: GGTGTCCTTGTTCTCGTTGGTT; 
                     added: 2020-07-07"
     primer_bind     2713..2732
                     /label=MTp302_ORF2_1000f
                     /note="color: black; sequence: CAGCTGAAGGAGCTGGAGAA; added:
                     2021-01-28"
     primer_bind     3204..3223
                     /label=MTp303_ORF2_1500f
                     /note="color: black; sequence: CTACAAGGAGGAGCTGGTGC; added:
                     2021-01-28"
     primer_bind     3703..3720
                     /label=MTp304_ORF2_2000f
                     /note="color: black; sequence: GCCTTCCCCCTGAAGACC; added: 
                     2021-01-28"
     misc_feature    3860..3863
                     /label=D702 RT
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     4211..4230
                     /label=MTp305_ORF2_2500f
                     /note="color: black; sequence: CCAAGGTGATCTACCGCTTC; added:
                     2021-01-28"
     primer_bind     4704..4721
                     /label=MTp306_ORF2_3000f
                     /note="color: black; sequence: CACCATCCAGGACATCGG; added: 
                     2021-01-28"
     primer_bind     5210..5227
                     /label=MTp307_ORF2_3500f
                     /note="color: black; sequence: CCCTGTGGAAGAGCGTGT; added: 
                     2021-01-28"
     CDS             5605..5628
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /note="color: #cc99b2
                     Cleavage site after base 5622"
                     /translation="LEVLFQGP"
     CDS             5629..5694
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /note="color: #cc99b2
                     Cleavage site after base 5694"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(5676..5697)
                     /label=MTp896v2_L1RP-Lenti-Rev_v2
                     /note="color: black; sequence: 
                     ccaacatggccGATATCGGTACCcctgcaggCTActtgtcatcgtcatccttg; 
                     added: 2023-03-14"
     misc_feature    5696..5902
                     /label=3'UTR
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(5865..5912)
                     /label=MTp970_L1-3UTR-WenLenti-Rev-Xlong
                     /note="color: black; sequence: 
                     cttagctCGCAGGGGAGGTGGTCTGGATCCGTTTAAACATTATACTCTAAGTTTTAGGG
                     TACATGTGCACATTGTG; added: 2023-06-28"
     primer_bind     complement(5868..5902)
                     /label=MTp937_L1RP-LentiRev-IRES-v2
                     /note="color: black; sequence: 
                     tctggaataagctcgagacactagtATGCATattatactctaagttttagggtacatgt
                     gcacatt; added: 2023-04-19"
     primer_bind     complement(5869..5902)
                     /label=MTp938v2_L1RP-LentiRev3
                     /note="color: black; sequence: 
                     tgtcgactcagtcagtcaaGCATGCaattatactctaagttttagggtacatgtgcaca
                     t; added: 2023-04-20"
     primer_bind     complement(5874..5912)
                     /label=MTp960_L1RP-WenLenti-Rev
                     /note="color: black; sequence: 
                     CGCAGGGGAGGTGGTCTGGATCCGTTTAAACATTATACTCTAAGTTTTAGGGTACATGT
                     G; added: 2023-06-21"
     primer_bind     complement(5875..5908)
                     /label=MTp902_ORFeus-Rev-C
                     /note="color: black; sequence: 
                     tggccaacatggccGATATCGGTACCGGATCCATTATACTCTAAGTTTTAGGGTACATG
                     T; added: 2023-02-23"
     primer_bind     complement(5880..5908)
                     /label=MTp906_ORFeus-Rev-Lenti2
                     /note="color: black; sequence: 
                     ccaacatggccGATATCGGTACCcctgcaggGGATCCATTATACTCTAAGTTTTAGGGT
                     A; added: 2023-02-23"
     misc_feature    5904..5908
                     /label=MCS
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    5917..6158
                     /label=SV40
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    6576..8551
                     /label=OriP
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             complement(8852..10777)
                     /label=EBNA-1
                     /note="color: #993366; direction: LEFT"
     CDS             11403..12263
                     /label=Amp
                     /note="color: #993366; direction: RIGHT"
     rep_origin      12272..13047
                     /direction=RIGHT
                     /label=pUC Ori
                     /note="color: #ffff00"
     promoter        13532..13815
                     /label=pTK--
                     /note="color: #ffffff; direction: RIGHT"
     CDS             13756..14355
                     /label=Puro
                     /note="color: #993366; direction: RIGHT"
     promoter        14858..15026
                     /label=TKpA
                     /note="color: #ffffff; direction: RIGHT"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatctc tagaagctgg gtaccagctg ctagcaagct tgctagcggc cgcgtttaaa
      661 cttaattaag ccaccatggg caagaagcag aaccgcaaga ccggcaacag caagacccag
      721 agcgccagcc ccccccccaa ggagcgcagc agcagccccg ccaccgagca gagctggatg
      781 gagaacgact tcgacgagct gcgcgaggag ggcttccgcc gcagcaacta cagcgagctg
      841 cgcgaggaca tccagaccaa gggcaaggag gtggagaact tcgagaagaa cctggaggag
      901 tgcatcaccc gcatcaccaa caccgagaag tgcctgaagg agctgatgga gctgaagacc
      961 aaggcccgcg agctgcgcga ggagtgccgc agcctgcgca gccgctgcga ccaattggag
     1021 gagcgcgtga gcgccatgga ggacgagatg aacgagatga agcgcgaggg caagttccgc
     1081 gagaagcgca tcaagcgcaa cgagcagagc ctgcaggaga tctgggacta cgtgaagcgc
     1141 cccaacctgc gcctgatcgg cgtgcccgag agcgacgtgg agaacggcac caagctggag
     1201 aacaccctgc aggacatcat ccaggagaac ttccccaacc tggcccgcca ggccaacgtg
     1261 cagatccagg agatccagcg caccccccag cgctacagca gccgccgcgc caccccccgc
     1321 cacatcatcg tgcgcttcac caaggtggag atgaaggaga agatgctgcg cgccgcccgc
     1381 gagaagggcc gcgtgaccct gaagggcaag cccatccgtc tcaccgccga cctgagcgcc
     1441 gagaccctgc aggcccgccg cgagtggggc cccatcttca acatcctgaa ggagaagaac
     1501 ttccagcccc gcatcagcta ccccgccaag ctgagcttca tcagcgaggg cgagatcaag
     1561 tacttcatcg acaagcagat gctgcgcgac ttcgtgacca cccgccccgc cctgaaggag
     1621 ctgctgaagg aggccctgaa catggagcgc aacaaccgct accagcccct gcagaaccac
     1681 gccaagatgt aaagaccatc gagactagga agaaactgca tcaactaatg agcaaaatca
     1741 ggcgcgccca tcataatgac cggcagcaac agccacatca ccatcctgac cctgaacatc
     1801 aacggcctga acagcgccat caagcgccac cgcctggcca gctggatcaa gagccaggac
     1861 cccagcgtgt gctgcatcca ggagacccac ctgacctgcc gcgacaccca ccgcctgaag
     1921 atcaagggct ggcgcaagat ctaccaggcc aacggcaagc agaagaaggc cggcgtggcc
     1981 atcctggtga gcgacaagac cgacttcaag cccaccaaga tcaagcgcga caaggagggc
     2041 cactacatca tggtgaaggg cagcatccag caggaggagc tgaccatcct gaacatctac
     2101 gcccccaaca ccggtgcccc ccgcttcatc aagcaggtgc tgagcgacct gcagcgcgac
     2161 ctggacagcc acaccctgat catgggcgac ttcaacaccc ccctgagcac cctggaccgc
     2221 agcacccgcc agaaggtgaa caaggacacc caggagctga acagcgccct gcaccaggcc
     2281 gacctgatcg acatctaccg caccctgcac cccaagagca ccgagtacac cttcttcagc
     2341 gccccccacc acacctacag caagatcgac cacatcgtgg gcagcaaggc cctgctgagc
     2401 aagtgcaagc gcaccgagat catcaccaac tacctgagcg accacagcgc catcaagctg
     2461 gagctgcgca tcaagaacct gacccagagc cgcagcacca cctggaagct gaacaacctg
     2521 ctgctgaacg actactgggt gcacaacgag atgaaggccg agatcaagat gttcttcgaa
     2581 accaacgaga acaaggacac cacctaccag aacctgtggg acgccttcaa ggccgtgtgc
     2641 cgcggcaagt tcatcgccct gaacgcctac aagcgcaagc aggagcgcag caagatcgac
     2701 accctgacca gccagctgaa ggagctggag aagcaggagc agacccacag caaggccagc
     2761 cgccgccagg agatcaccaa gatccgcgcc gagctgaagg agatcgagac ccagaagacc
     2821 ctgcagaaga tcaacgagtc gcgaagctgg ttcttcgagc gcatcaacaa gatcgaccgc
     2881 cccctggccc gcctgatcaa gaagaagcgc gagaagaacc agatcgacac catcaagaac
     2941 gacaagggcg acatcaccac cgaccccacc gagatccaga ccaccatccg cgagtactac
     3001 aagcacctgt acgccaacaa gctggagaac ctggaggaga tggacacctt cctggacacc
     3061 tacaccctgc cccgcctgaa ccaggaggag gtggagagcc tgaaccgccc catcaccggc
     3121 agcgagatcg tggccatcat caacagcctg cccaccaaga agagccccgg ccccgacggc
     3181 ttcaccgccg agttctacca gcgctacaag gaggagctgg tgcccttcct gctgaagctg
     3241 ttccaaagca tcgagaagga gggcatcctg cccaacagct tctacgaggc cagcatcatc
     3301 ctgatcccca agcccggccg cgacaccacc aagaaggaga acttccgccc catcagcctg
     3361 atgaacatcg acgccaagat cctgaacaag atcctggcca accgcatcca gcagcacatc
     3421 aagaagctga tccaccacga ccaggtgggc ttcatccccg ggatgcaggg ctggttcaac
     3481 atccgcaaga gcatcaacgt gatccagcac atcaaccgcg ccaaggacaa gaaccacatg
     3541 atcatcagca tcgacgccga gaaggccttc gacaagatcc agcagccctt catgctgaag
     3601 accctgaaca agctgggcat cgacggcacc tacttcaaga tcatccgcgc catctacgac
     3661 aagcccaccg ccaacatcat cctgaacggc cagaagctgg aggccttccc cctgaagacc
     3721 ggcacgcgtc agggctgccc cctgagcccc ctgctgttca acatcgtgct ggaggtgctg
     3781 gcccgcgcca tccgccagga gaaggagatc aagggcatcc agctgggcaa ggaggaggtg
     3841 aagctgagcc tgttcgccga cgacatgatc gtgtacctgg agaaccccat cgtgagcgcc
     3901 cagaacctgc tgaagctgat cagcaacttc agcaaggtga gcggctacaa gatcaacgtg
     3961 cagaagagcc aggccttcct gtacaccaac aaccgccaga ccgagagcca gatcatgggc
     4021 gagctgccct tcaccatcgc tagcaagcgc atcaagtacc tgggcatcca gctgacccgc
     4081 gacgtgaagg acctgttcaa ggagaactac aagcccctgc tgaaggagat caaggaggag
     4141 accaacaagt ggaagaacat cccctgcagc tgggtgggcc gcatcaacat cgtgaagatg
     4201 gccatcctgc ccaaggtgat ctaccgcttc aacgccatcc ccatcaagct gcccatgacc
     4261 ttcttcaccg agctggagaa gaccaccctg aagttcatct ggaaccagaa gcgcgcccgc
     4321 atcgccaaga gcatcctgag ccagaagaac aaggccggcg gcatcaccct gcccgacttc
     4381 aagctgtact acaaggccac cgtgaccaag accgcctggt actggtacca gaaccgcgat
     4441 atcgaccagt ggaaccgcac cgagcccagc gagatcatgc cccacatcta caactacctg
     4501 atcttcgaca agcccgagaa gaacaagcag tggggcaagg acagcctgtt caacaagtgg
     4561 tgctgggaga actggctggc catctgccgc aagctgaagc tggacccctt cctgaccccc
     4621 tacaccaaga tcaacagccg ctggatcaag gacctgaacg tgaagcccaa gaccatcaag
     4681 accctggagg agaacctggg catcaccatc caggacatcg gcgtgggcaa ggacttcatg
     4741 agcaagaccc ccaaggccat ggccaccaag gacaagatcg acaagtggga cctgatcaag
     4801 ctgaagagct tctgcaccgc caaggagacc accatccgcg tgaaccgcca gcccaccacc
     4861 tgggagaaga tcttcgccac ctacagcagc gacaagggcc tgatcagccg catctacaac
     4921 gagctgaagc agatctacaa gaagaagacc aacaacccca tcaagaagtg ggccaaggac
     4981 atgaaccgcc acttcagcaa ggaggacatc tacgccgcca agaagcatat gaagaagtgc
     5041 agcagcagcc tggccatccg cgagatgcag atcaagacca ccatgcgcta ccacctgacc
     5101 cccgtgcgca tggccatcat caagaagagc ggcaacaacc gctgctggcg cggctgcggc
     5161 gagatcggca ccctgctgca ctgctggtgg gactgcaagc tggtgcagcc cctgtggaag
     5221 agcgtgtggc gcttcctgcg cgacctggag ctggagatcc ccttcgaccc cgccatcccc
     5281 ctgctgggca tctaccccaa cgagtacaag agctgctgct acaaggacac ctgcacccgc
     5341 atgttcatcg ccgccctgtt caccatcgcc aagacctgga accagcccaa gtgccccacc
     5401 atgatcgatt ggatcaagaa gatgtggcac atctacacca tggagtacta cgccgccatc
     5461 aagaacgacg agttcatcag cttcgtgggc acctggatga agctggagac catcatcctg
     5521 agcaagctga gccaggagca gaagaccaag caccgcatct tcagcctgat cggcggcaac
     5581 ggcggcggca gcggcggcgg cagcctggaa gttctgttcc aggggcccga ctacaaagac
     5641 catgacggtg attataaaga tcatgacatc gattacaagg atgacgatga caagtagaca
     5701 atgagatcac atggacacag gaaggggaat atcacactct ggggactgtg gtggggtcgg
     5761 gggagggggg agggatagca ttgggagata tacctaatgc tagatgacac attagtgggt
     5821 gcagcgcacc agcatggcac atgtatacat atgtaactaa cctgcacaat gtgcacatgt
     5881 accctaaaac ttagagtata atggatccag acatgataag atacattgat gagtttggac
     5941 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     6001 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     6061 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     6121 aatgtggtat ggctgattat gatccggctg cctcgcgcgt ttcggtgatg acggtgaaaa
     6181 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     6241 cagacaagcc cgtcaggcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaggtc
     6301 gatcgactct agaggatcga tgccccgccc cggacgaact aaacctgact acgacatctc
     6361 tgccccttct tcgcggggca gtgcatgtaa tcccttcagt tggttggtac aacttgccaa
     6421 ctgggccctg ttccacatgt gacacggggg gggaccaaac acaaaggggt tctctgactg
     6481 tagttgacat ccttataaat ggatgtgcac atttgccaac actgagtggc tttcatcctg
     6541 gagcagactt tgcagtctgt ggactgcaac acaacattgc ctttatgtgt aactcttggc
     6601 tgaagctctt acaccaatgc tgggggacat gtacctccca ggggcccagg aagactacgg
     6661 gaggctacac caacgtcaat cagaggggcc tgtgtagcta ccgataagcg gaccctcaag
     6721 agggcattag caatagtgtt tataaggccc ccttgttaac cctaaacggg tagcatatgc
     6781 ttcccgggta gtagtatata ctatccagac taaccctaat tcaatagcat atgttaccca
     6841 acgggaagca tatgctatcg aattagggtt agtaaaaggg tcctaaggaa cagcgatatc
     6901 tcccacccca tgagctgtca cggttttatt tacatggggt caggattcca cgagggtagt
     6961 gaaccatttt agtcacaagg gcagtggctg aagatcaagg agcgggcagt gaactctcct
     7021 gaatcttcgc ctgcttcttc attctccttc gtttagctaa tagaataact gctgagttgt
     7081 gaacagtaag gtgtatgtga ggtgctcgaa aacaaggttt caggtgacgc ccccagaata
     7141 aaatttggac ggggggttca gtggtggcat tgtgctatga caccaatata accctcacaa
     7201 accccttggg caataaatac tagtgtagga atgaaacatt ctgaatatct ttaacaatag
     7261 aaatccatgg ggtggggaca agccgtaaag actggatgtc catctcacac gaatttatgg
     7321 ctatgggcaa cacataatcc tagtgcaata tgatactggg gttattaaga tgtgtcccag
     7381 gcagggacca agacaggtga accatgttgt tacactctat ttgtaacaag gggaaagaga
     7441 gtggacgccg acagcagcgg actccactgg ttgtctctaa cacccccgaa aattaaacgg
     7501 ggctccacgc caatggggcc cataaacaaa gacaagtggc cactcttttt tttgaaattg
     7561 tggagtgggg gcacgcgtca gcccccacac gccgccctgc ggttttggac tgtaaaataa
     7621 gggtgtaata acttggctga ttgtaacccc gctaaccact gcggtcaaac cacttgccca
     7681 caaaaccact aatggcaccc cggggaatac ctgcataagt aggtgggcgg gccaagatag
     7741 gggcgcgatt gctgcgatct ggaggacaaa ttacacacac ttgcgcctga gcgccaagca
     7801 cagggttgtt ggtcctcata ttcacgaggt cgctgagagc acggtgggct aatgttgcca
     7861 tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc tatatctggg
     7921 tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg
     7981 tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg
     8041 tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg
     8101 tagcatatgc tatcctaata gagattaggg tagtatatgc tatcctaatt tatatctggg
     8161 tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc
     8221 atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc
     8281 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc
     8341 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     8401 atatgctatc ctaatctgta tccgggtagc atatgctatc ctcatgcata tacagtcagc
     8461 atatgatacc cagtagtaga gtgggagtgc tatcctttgc atatgccgcc acctcccaag
     8521 ggggcgtgaa ttttcgctgc ttgtcctttt cctgctggtt gctcccattc ttaggtgaat
     8581 ttaaggaggc caggctaaag ccgtcgcatg tctgattgct caccaggtaa atgtcgctaa
     8641 tgttttccaa cgcgagaagg tgttgagcgc ggagctgagt gacgtgacaa catgggtatg
     8701 cccaattgcc ccatgttggg aggacgaaaa tggtgacaag acagatggcc agaaatacac
     8761 caacagcacg catgatgtct actggggatt tattctttag tgcgggggaa tacacggctt
     8821 ttaatacgat tgagggcgtc tcctaacaag ttacatcact cctgcccttc ctcaccctca
     8881 tctccatcac ctccttcatc tccgtcatct ccgtcatcac cctccgcggc agccccttcc
     8941 accataggtg gaaaccaggg aggcaaatct actccatcgt caaagctgca cacagtcacc
     9001 ctgatattgc aggtaggagc gggctttgtc ataacaaggt ccttaatcgc atccttcaaa
     9061 acctcagcaa atatatgagt ttgtaaaaag accatgaaat aacagacaat ggactccctt
     9121 agcgggccag gttgtgggcc gggtccaggg gccattccaa aggggagacg actcaatggt
     9181 gtaagacgac attgtggaat agcaagggca gttcctcgcc ttaggttgta aagggaggtc
     9241 ttactacctc catatacgaa cacaccggcg acccaagttc cttcgtcggt agtcctttct
     9301 acgtgactcc tagccaggag agctcttaaa ccttctgcaa tgttctcaaa tttcgggttg
     9361 gaacctcctt gaccacgatg cttttccaaa ccaccctcct tttttgcgcc ctgcctccat
     9421 caccctgacc ccggggtcca gtgcttgggc cttctcctgg gtcatctgcg gggccctgct
     9481 ctatcgctcc cgggggcacg tcaggctcac catctgggcc accttcttgg tggtattcaa
     9541 aataatcggc ttcccctaca gggtggaaaa atggccttct acctggaggg ggcctgcgcg
     9601 gtggagaccc ggatgatgat gactgactac tgggactcct gggcctcttt tctccacgtc
     9661 cacgacctct ccccctggct ctttcacgac ttccccccct ggctctttca cgtcctctac
     9721 cccggcggcc tccactacct cctcgacccc ggcctccact acctcctcga ccccggcctc
     9781 cactgcctcc tcgaccccgg cctccacctc ctgctcctgc ccctcctgct cctgcccctc
     9841 ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctgctcctg
     9901 cccctcctgc ccctcctgct cctgcccctc ctgcccctcc tcctgctcct gcccctcctg
     9961 cccctcctcc tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct gcccctcctg
    10021 ctcctgcccc tcctgcccct cctgctcctg cccctcctgc tcctgcccct cctgctcctg
    10081 cccctcctgc tcctgcccct cctgcccctc ctgcccctcc tcctgctcct gcccctcctg
    10141 ctcctgcccc tcctgcccct cctgcccctc ctgctcctgc ccctcctcct gctcctgccc
    10201 ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct cctgctcctg
    10261 cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc tcctcctgct cctgcccctc
    10321 ctgcccctcc tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctgccc
    10381 ctcctcctgc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gcccctcctg
    10441 cccctcctcc tgctcctgcc cctcctcctg ctcctgcccc tcctgctcct gcccctcccg
    10501 ctcctgctcc tgctcctgtt ccaccgtggg tccctttgca gccaatgcaa cttggacgtt
    10561 tttggggtct ccggacacca tctctatgtc ttggccctga tcctgagccg cccggggctc
    10621 ctggtcttcc gcctcctcgt cctcgtcctc ttccccgtcc tcgtccatgg ttatcacccc
    10681 ctcttctttg aggtccactg ccgccggagc cttctggtcc agatgtgtct cccttctctc
    10741 ctaggccatt tccaggtcct gtacctggcc cctcgtcaga catgattcac actaaaagag
    10801 atcaatagac atctttatta gacgacgctc agtgaataca gggagtgcag actcctgccc
    10861 cctccaacag cccccccacc ctcatcccct tcatggtcgc tgtcagacag atccaggtct
    10921 gaaaattccc catcctccga accatcctcg tcctcatcac caattactcg cagcccggaa
    10981 aactcccgct gaacatcctc aagatttgcg tcctgagcct caagccaggc ctcaaattcc
    11041 tcgtccccct ttttgctgga cggtagggat ggggattctc gggacccctc ctcttcctct
    11101 tcaaggtcac cagacagaga tgctactggg gcaacggaag aaaagctggg tgcggcctgt
    11161 gaggatcagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc
    11221 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
    11281 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
    11341 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
    11401 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
    11461 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
    11521 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
    11581 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
    11641 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    11701 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    11761 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    11821 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    11881 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    11941 ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    12001 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    12061 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    12121 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    12181 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    12241 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    12301 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    12361 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    12421 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    12481 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    12541 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
    12601 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    12661 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    12721 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    12781 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    12841 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    12901 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    12961 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    13021 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttgaa
    13081 gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc
    13141 cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgaat
    13201 tcgtcgacct cgaaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
    13261 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca
    13321 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca
    13381 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
    13441 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag
    13501 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc
    13561 tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc
    13621 ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg
    13681 tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcat ccatctagat
    13741 ctcgagcagc tgaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc
    13801 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
    13861 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
    13921 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
    13981 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
    14041 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
    14101 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
    14161 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
    14221 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
    14281 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
    14341 acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc
    14401 gcacgacccc atgcatcgat gatatcagat ccccgggatg cagaaattga tgatctatta
    14461 aacaataaag atgtccacta aaatggaagt ttttcctgtc atactttgtt aagaagggtg
    14521 agaacagagt acctacattt tgaatggaag gattggagct acgggggtgg gggtggggtg
    14581 ggattagata aatgcctgct ctttactgaa ggctctttac tattgcttta tgataatgtt
    14641 tcatagttgg atatcataat ttaaacaagc aaaaccaaat taagggccag ctcattcctc
    14701 ccactcatga tctatagatc tatagatctc tcgtgggatc attgtttttc tcttgattcc
    14761 cactttgtgg ttctaagtac tgtggtttcc aaatgtgtca gtttcatagc ctgaagaacg
    14821 agatcagcag cctctgttcc acatacactt cattctcagt attgttttgc caagttctaa
    14881 ttccatcaga agctggtcga ccaattctca tgtttgacag cttatcatcg cagatccggg
    14941 caacgttgtt gccattgctg caggcgcaga actggtaggt atggaagatc tatacattga
    15001 atcaatattg gcaattagcc atattagtca ttggttatat agcataaatc aatattggct
    15061 attggccatt gcatacgttg tatctatatc ataatatgta catttatatt ggctcatgtc
    15121 caatatgacc gccat
//
