LOCUS       pMT647_human_L1       14044 bp DNA     circular SYN 03-DEC-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14044)
  AUTHORS   Marty Taylor
  TITLE     Direct Submission
  JOURNAL   Exported Dec 3, 2023 from SnapGene 7.0.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        384..587
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    619..651
                     /label=MCS
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     631..682
                     /label=MTp776_ORF2-pcDNA34-Fwd
                     /note="color: black; sequence: 
                     ACCGATCCAGCCTCCGGACTCTAGAAAGCTTGCCACCATGACCGGCAGCAACAGC; 
                     added: 2022-07-15"
     CDS             665..4604
                     /codon_start=1
                     /label=Orf2-PPX-3xFlag
                     /note="color: #993366"
                     /translation="MTGSNSHITILTLNINGLNSAIKRHRLASWIKSQDPSVCCIQETH
                     LTCRDTHRLKIKGWRKIYQANGKQKKAGVAILVSDKTDFKPTKIKRDKEGHYIMVKGSI
                     QQEELTILNIYAPNTGAPRFIKQVLSDLQRDLDSHTLIMGDFNTPLSTLDRSTRQKVNK
                     DTQELNSALHQADLIDIYRTLHPKSTEYTFFSAPHHTYSKIDHIVGSKALLSKCKRTEI
                     ITNYLSDHSAIKLELRIKNLTQSRSTTWKLNNLLLNDYWVHNEMKAEIKMFFETNENKD
                     TTYQNLWDAFKAVCRGKFIALNAYKRKQERSKIDTLTSQLKELEKQEQTHSKASRRQEI
                     TKIRAELKEIETQKTLQKINESRSWFFERINKIDRPLARLIKKKREKNQIDTIKNDKGD
                     ITTDPTEIQTTIREYYKHLYANKLENLEEMDTFLDTYTLPRLNQEEVESLNRPITGSEI
                     VAIINSLPTKKSPGPDGFTAEFYQRYKEELVPFLLKLFQSIEKEGILPNSFYEASIILI
                     PKPGRDTTKKENFRPISLMNIDAKILNKILANRIQQHIKKLIHHDQVGFIPGMQGWFNI
                     RKSINVIQHINRAKDKNHMIISIDAEKAFDKIQQPFMLKTLNKLGIDGTYFKIIRAIYD
                     KPTANIILNGQKLEAFPLKTGTRQGCPLSPLLFNIVLEVLARAIRQEKEIKGIQLGKEE
                     VKLSLFADDMIVYLENPIVSAQNLLKLISNFSKVSGYKINVQKSQAFLYTNNRQTESQI
                     MGELPFTIASKRIKYLGIQLTRDVKDLFKENYKPLLKEIKEETNKWKNIPCSWVGRINI
                     VKMAILPKVIYRFNAIPIKLPMTFFTELEKTTLKFIWNQKRARIAKSILSQKNKAGGIT
                     LPDFKLYYKATVTKTAWYWYQNRDIDQWNRTEPSEIMPHIYNYLIFDKPEKNKQWGKDS
                     LFNKWCWENWLAICRKLKLDPFLTPYTKINSRWIKDLNVKPKTIKTLEENLGITIQDIG
                     VGKDFMSKTPKAMATKDKIDKWDLIKLKSFCTAKETTIRVNRQPTTWEKIFATYSSDKG
                     LISRIYNELKQIYKKKTNNPIKKWAKDMNRHFSKEDIYAAKKHMKKCSSSLAIREMQIK
                     TTMRYHLTPVRMAIIKKSGNNRCWRGCGEIGTLLHCWWDCKLVQPLWKSVWRFLRDLEL
                     EIPFDPAIPLLGIYPNEYKSCCYKDTCTRMFIAALFTIAKTWNQPKCPTMIDWIKKMWH
                     IYTMEYYAAIKNDEFISFVGTWMKLETIILSKLSQEQKTKHRIFSLIGGNGGGSGGGSL
                     EVLFQGPDYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     665..682
                     /label=MTp787_ORF2-pRK5-Fwd
                     /note="color: black; sequence: 
                     AAGCTTCTCGAGGCCGCCACCATGACCGGCAGCAACAGC; added: 2022-07-15"
     primer_bind     complement(1254..1273)
                     /label=MTp301_ORF2_500_rev
                     /note="color: black; sequence: CTTGCTGTAGGTGTGGTGGG; added:
                     2021-01-28"
     primer_bind     1254..1273
                     /label=MTp308_ORF2_500f_v2
                     /note="color: black; sequence: CCCACCACACCTACAGCAAG; added:
                     2021-01-28"
     primer_bind     1622..1641
                     /label=MTp302_ORF2_1000f
                     /note="color: black; sequence: CAGCTGAAGGAGCTGGAGAA; added:
                     2021-01-28"
     primer_bind     2113..2132
                     /label=MTp303_ORF2_1500f
                     /note="color: black; sequence: CTACAAGGAGGAGCTGGTGC; added:
                     2021-01-28"
     primer_bind     2612..2629
                     /label=MTp304_ORF2_2000f
                     /note="color: black; sequence: GCCTTCCCCCTGAAGACC; added: 
                     2021-01-28"
     primer_bind     3120..3139
                     /label=MTp305_ORF2_2500f
                     /note="color: black; sequence: CCAAGGTGATCTACCGCTTC; added:
                     2021-01-28"
     primer_bind     3613..3630
                     /label=MTp306_ORF2_3000f
                     /note="color: black; sequence: CACCATCCAGGACATCGG; added: 
                     2021-01-28"
     primer_bind     4119..4136
                     /label=MTp307_ORF2_3500f
                     /note="color: black; sequence: CCCTGTGGAAGAGCGTGT; added: 
                     2021-01-28"
     CDS             4514..4537
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /note="color: #cc99b2
                     Cleavage site after base 4531"
                     /translation="LEVLFQGP"
     CDS             4538..4603
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /note="color: #cc99b2
                     Cleavage site after base 4603"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(4578..4604)
                     /label=MTp786_ORF2-pRK5-Rev
                     /note="color: black; sequence: 
                     tacttagcggccGCTTATCACTTGTCATCGTCATCCTTGTAATCGA; added: 
                     2022-07-15"
     primer_bind     complement(4579..4606)
                     /label=MTp777_ORF2-pcDNA34-Rev
                     /note="color: black; sequence: 
                     ATTTTGTAATCCAGAGGTTGATTGGATCCTCACTACTTGTCATCGTCATCCTTGTAATC
                     G; added: 2022-07-12"
     misc_feature    4605..4811
                     /label=3'UTR
                     /note="color: #a6acb3; direction: RIGHT"
     polyA_signal    4813..4947
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      5674..7463
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
                     /note="color: #ffff00"
     CDS             complement(7761..9686)
                     /label=EBNA-1
                     /note="color: #993366; direction: LEFT"
     promoter        10213..10317
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             10318..11178
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 10318 .. 10386 / #ccffcc / signal sequence
                        2: 10387 .. 11178 / #ccffcc
                     Cleavage site after base 10386"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11349..11937
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     promoter        12132..12631
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        12441..12724
                     /label=pTK--
                     /note="color: #ffffff; direction: RIGHT"
     CDS             12671..13270
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     promoter        13767..13935
                     /label=TKpA
                     /note="color: #ffffff; direction: RIGHT"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatctc tagaagctgg gtaccagctg ctagcaagct tgctagcggc ccgcgcccat
      661 cataatgacc ggcagcaaca gccacatcac catcctgacc ctgaacatca acggcctgaa
      721 cagcgccatc aagcgccacc gcctggccag ctggatcaag agccaggacc ccagcgtgtg
      781 ctgcatccag gagacccacc tgacctgccg cgacacccac cgcctgaaga tcaagggctg
      841 gcgcaagatc taccaggcca acggcaagca gaagaaggcc ggcgtggcca tcctggtgag
      901 cgacaagacc gacttcaagc ccaccaagat caagcgcgac aaggagggcc actacatcat
      961 ggtgaagggc agcatccagc aggaggagct gaccatcctg aacatctacg cccccaacac
     1021 cggtgccccc cgcttcatca agcaggtgct gagcgacctg cagcgcgacc tggacagcca
     1081 caccctgatc atgggcgact tcaacacccc cctgagcacc ctggaccgca gcacccgcca
     1141 gaaggtgaac aaggacaccc aggagctgaa cagcgccctg caccaggccg acctgatcga
     1201 catctaccgc accctgcacc ccaagagcac cgagtacacc ttcttcagcg ccccccacca
     1261 cacctacagc aagatcgacc acatcgtggg cagcaaggcc ctgctgagca agtgcaagcg
     1321 caccgagatc atcaccaact acctgagcga ccacagcgcc atcaagctgg agctgcgcat
     1381 caagaacctg acccagagcc gcagcaccac ctggaagctg aacaacctgc tgctgaacga
     1441 ctactgggtg cacaacgaga tgaaggccga gatcaagatg ttcttcgaaa ccaacgagaa
     1501 caaggacacc acctaccaga acctgtggga cgccttcaag gccgtgtgcc gcggcaagtt
     1561 catcgccctg aacgcctaca agcgcaagca ggagcgcagc aagatcgaca ccctgaccag
     1621 ccagctgaag gagctggaga agcaggagca gacccacagc aaggccagcc gccgccagga
     1681 gatcaccaag atccgcgccg agctgaagga gatcgagacc cagaagaccc tgcagaagat
     1741 caacgagtcg cgaagctggt tcttcgagcg catcaacaag atcgaccgcc ccctggcccg
     1801 cctgatcaag aagaagcgcg agaagaacca gatcgacacc atcaagaacg acaagggcga
     1861 catcaccacc gaccccaccg agatccagac caccatccgc gagtactaca agcacctgta
     1921 cgccaacaag ctggagaacc tggaggagat ggacaccttc ctggacacct acaccctgcc
     1981 ccgcctgaac caggaggagg tggagagcct gaaccgcccc atcaccggca gcgagatcgt
     2041 ggccatcatc aacagcctgc ccaccaagaa gagccccggc cccgacggct tcaccgccga
     2101 gttctaccag cgctacaagg aggagctggt gcccttcctg ctgaagctgt tccaaagcat
     2161 cgagaaggag ggcatcctgc ccaacagctt ctacgaggcc agcatcatcc tgatccccaa
     2221 gcccggccgc gacaccacca agaaggagaa cttccgcccc atcagcctga tgaacatcga
     2281 cgccaagatc ctgaacaaga tcctggccaa ccgcatccag cagcacatca agaagctgat
     2341 ccaccacgac caggtgggct tcatccccgg gatgcagggc tggttcaaca tccgcaagag
     2401 catcaacgtg atccagcaca tcaaccgcgc caaggacaag aaccacatga tcatcagcat
     2461 cgacgccgag aaggccttcg acaagatcca gcagcccttc atgctgaaga ccctgaacaa
     2521 gctgggcatc gacggcacct acttcaagat catccgcgcc atctacgaca agcccaccgc
     2581 caacatcatc ctgaacggcc agaagctgga ggccttcccc ctgaagaccg gcacgcgtca
     2641 gggctgcccc ctgagccccc tgctgttcaa catcgtgctg gaggtgctgg cccgcgccat
     2701 ccgccaggag aaggagatca agggcatcca gctgggcaag gaggaggtga agctgagcct
     2761 gttcgccgac gacatgatcg tgtacctgga gaaccccatc gtgagcgccc agaacctgct
     2821 gaagctgatc agcaacttca gcaaggtgag cggctacaag atcaacgtgc agaagagcca
     2881 ggccttcctg tacaccaaca accgccagac cgagagccag atcatgggcg agctgccctt
     2941 caccatcgct agcaagcgca tcaagtacct gggcatccag ctgacccgcg acgtgaagga
     3001 cctgttcaag gagaactaca agcccctgct gaaggagatc aaggaggaga ccaacaagtg
     3061 gaagaacatc ccctgcagct gggtgggccg catcaacatc gtgaagatgg ccatcctgcc
     3121 caaggtgatc taccgcttca acgccatccc catcaagctg cccatgacct tcttcaccga
     3181 gctggagaag accaccctga agttcatctg gaaccagaag cgcgcccgca tcgccaagag
     3241 catcctgagc cagaagaaca aggccggcgg catcaccctg cccgacttca agctgtacta
     3301 caaggccacc gtgaccaaga ccgcctggta ctggtaccag aaccgcgata tcgaccagtg
     3361 gaaccgcacc gagcccagcg agatcatgcc ccacatctac aactacctga tcttcgacaa
     3421 gcccgagaag aacaagcagt ggggcaagga cagcctgttc aacaagtggt gctgggagaa
     3481 ctggctggcc atctgccgca agctgaagct ggaccccttc ctgaccccct acaccaagat
     3541 caacagccgc tggatcaagg acctgaacgt gaagcccaag accatcaaga ccctggagga
     3601 gaacctgggc atcaccatcc aggacatcgg cgtgggcaag gacttcatga gcaagacccc
     3661 caaggccatg gccaccaagg acaagatcga caagtgggac ctgatcaagc tgaagagctt
     3721 ctgcaccgcc aaggagacca ccatccgcgt gaaccgccag cccaccacct gggagaagat
     3781 cttcgccacc tacagcagcg acaagggcct gatcagccgc atctacaacg agctgaagca
     3841 gatctacaag aagaagacca acaaccccat caagaagtgg gccaaggaca tgaaccgcca
     3901 cttcagcaag gaggacatct acgccgccaa gaagcatatg aagaagtgca gcagcagcct
     3961 ggccatccgc gagatgcaga tcaagaccac catgcgctac cacctgaccc ccgtgcgcat
     4021 ggccatcatc aagaagagcg gcaacaaccg ctgctggcgc ggctgcggcg agatcggcac
     4081 cctgctgcac tgctggtggg actgcaagct ggtgcagccc ctgtggaaga gcgtgtggcg
     4141 cttcctgcgc gacctggagc tggagatccc cttcgacccc gccatccccc tgctgggcat
     4201 ctaccccaac gagtacaaga gctgctgcta caaggacacc tgcacccgca tgttcatcgc
     4261 cgccctgttc accatcgcca agacctggaa ccagcccaag tgccccacca tgatcgattg
     4321 gatcaagaag atgtggcaca tctacaccat ggagtactac gccgccatca agaacgacga
     4381 gttcatcagc ttcgtgggca cctggatgaa gctggagacc atcatcctga gcaagctgag
     4441 ccaggagcag aagaccaagc accgcatctt cagcctgatc ggcggcaacg gcggcggcag
     4501 cggcggcggc agcctggaag ttctgttcca ggggcccgac tacaaagacc atgacggtga
     4561 ttataaagat catgacatcg attacaagga tgacgatgac aagtagacaa tgagatcaca
     4621 tggacacagg aaggggaata tcacactctg gggactgtgg tggggtcggg ggagggggga
     4681 gggatagcat tgggagatat acctaatgct agatgacaca ttagtgggtg cagcgcacca
     4741 gcatggcaca tgtatacata tgtaactaac ctgcacaatg tgcacatgta ccctaaaact
     4801 tagagtataa tggatccaga catgataaga tacattgatg agtttggaca aaccacaact
     4861 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     4921 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     4981 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg
     5041 gctgattatg atccggctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     5101 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     5161 gtcaggcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgaggtcg atcgactcta
     5221 gaggatcgat gccccgcccc ggacgaacta aacctgacta cgacatctct gccccttctt
     5281 cgcggggcag tgcatgtaat cccttcagtt ggttggtaca acttgccaac tgggccctgt
     5341 tccacatgtg acacgggggg ggaccaaaca caaaggggtt ctctgactgt agttgacatc
     5401 cttataaatg gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg agcagacttt
     5461 gcagtctgtg gactgcaaca caacattgcc tttatgtgta actcttggct gaagctctta
     5521 caccaatgct gggggacatg tacctcccag gggcccagga agactacggg aggctacacc
     5581 aacgtcaatc agaggggcct gtgtagctac cgataagcgg accctcaaga gggcattagc
     5641 aatagtgttt ataaggcccc cttgttaacc ctaaacgggt agcatatgct tcccgggtag
     5701 tagtatatac tatccagact aaccctaatt caatagcata tgttacccaa cgggaagcat
     5761 atgctatcga attagggtta gtaaaagggt cctaaggaac agcgatatct cccaccccat
     5821 gagctgtcac ggttttattt acatggggtc aggattccac gagggtagtg aaccatttta
     5881 gtcacaaggg cagtggctga agatcaagga gcgggcagtg aactctcctg aatcttcgcc
     5941 tgcttcttca ttctccttcg tttagctaat agaataactg ctgagttgtg aacagtaagg
     6001 tgtatgtgag gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa aatttggacg
     6061 gggggttcag tggtggcatt gtgctatgac accaatataa ccctcacaaa ccccttgggc
     6121 aataaatact agtgtaggaa tgaaacattc tgaatatctt taacaataga aatccatggg
     6181 gtggggacaa gccgtaaaga ctggatgtcc atctcacacg aatttatggc tatgggcaac
     6241 acataatcct agtgcaatat gatactgggg ttattaagat gtgtcccagg cagggaccaa
     6301 gacaggtgaa ccatgttgtt acactctatt tgtaacaagg ggaaagagag tggacgccga
     6361 cagcagcgga ctccactggt tgtctctaac acccccgaaa attaaacggg gctccacgcc
     6421 aatggggccc ataaacaaag acaagtggcc actctttttt ttgaaattgt ggagtggggg
     6481 cacgcgtcag cccccacacg ccgccctgcg gttttggact gtaaaataag ggtgtaataa
     6541 cttggctgat tgtaaccccg ctaaccactg cggtcaaacc acttgcccac aaaaccacta
     6601 atggcacccc ggggaatacc tgcataagta ggtgggcggg ccaagatagg ggcgcgattg
     6661 ctgcgatctg gaggacaaat tacacacact tgcgcctgag cgccaagcac agggttgttg
     6721 gtcctcatat tcacgaggtc gctgagagca cggtgggcta atgttgccat gggtagcata
     6781 tactacccaa atatctggat agcatatgct atcctaatct atatctgggt agcataggct
     6841 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     6901 atcctaattt atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     6961 atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct
     7021 atcctaatag agattagggt agtatatgct atcctaattt atatctgggt agcatatact
     7081 acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca tatgctatcc
     7141 taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc
     7201 taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc
     7261 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc
     7321 taatctgtat ccgggtagca tatgctatcc tcatgcatat acagtcagca tatgataccc
     7381 agtagtagag tgggagtgct atcctttgca tatgccgcca cctcccaagg gggcgtgaat
     7441 tttcgctgct tgtccttttc ctgctggttg ctcccattct taggtgaatt taaggaggcc
     7501 aggctaaagc cgtcgcatgt ctgattgctc accaggtaaa tgtcgctaat gttttccaac
     7561 gcgagaaggt gttgagcgcg gagctgagtg acgtgacaac atgggtatgc ccaattgccc
     7621 catgttggga ggacgaaaat ggtgacaaga cagatggcca gaaatacacc aacagcacgc
     7681 atgatgtcta ctggggattt attctttagt gcgggggaat acacggcttt taatacgatt
     7741 gagggcgtct cctaacaagt tacatcactc ctgcccttcc tcaccctcat ctccatcacc
     7801 tccttcatct ccgtcatctc cgtcatcacc ctccgcggca gccccttcca ccataggtgg
     7861 aaaccaggga ggcaaatcta ctccatcgtc aaagctgcac acagtcaccc tgatattgca
     7921 ggtaggagcg ggctttgtca taacaaggtc cttaatcgca tccttcaaaa cctcagcaaa
     7981 tatatgagtt tgtaaaaaga ccatgaaata acagacaatg gactccctta gcgggccagg
     8041 ttgtgggccg ggtccagggg ccattccaaa ggggagacga ctcaatggtg taagacgaca
     8101 ttgtggaata gcaagggcag ttcctcgcct taggttgtaa agggaggtct tactacctcc
     8161 atatacgaac acaccggcga cccaagttcc ttcgtcggta gtcctttcta cgtgactcct
     8221 agccaggaga gctcttaaac cttctgcaat gttctcaaat ttcgggttgg aacctccttg
     8281 accacgatgc ttttccaaac caccctcctt ttttgcgccc tgcctccatc accctgaccc
     8341 cggggtccag tgcttgggcc ttctcctggg tcatctgcgg ggccctgctc tatcgctccc
     8401 gggggcacgt caggctcacc atctgggcca ccttcttggt ggtattcaaa ataatcggct
     8461 tcccctacag ggtggaaaaa tggccttcta cctggagggg gcctgcgcgg tggagacccg
     8521 gatgatgatg actgactact gggactcctg ggcctctttt ctccacgtcc acgacctctc
     8581 cccctggctc tttcacgact tccccccctg gctctttcac gtcctctacc ccggcggcct
     8641 ccactacctc ctcgaccccg gcctccacta cctcctcgac cccggcctcc actgcctcct
     8701 cgaccccggc ctccacctcc tgctcctgcc cctcctgctc ctgcccctcc tcctgctcct
     8761 gcccctcctg cccctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc
     8821 cctcctgctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc ccctcctcct
     8881 gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctgc tcctgcccct
     8941 cctgcccctc ctgctcctgc ccctcctgct cctgcccctc ctgctcctgc ccctcctgct
     9001 cctgcccctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc tcctgcccct
     9061 cctgcccctc ctgcccctcc tgctcctgcc cctcctcctg ctcctgcccc tcctgcccct
     9121 cctgcccctc ctcctgctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctcct
     9181 gctcctgccc ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct
     9241 cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc tcctcctgct
     9301 cctgcccctc ctcctgctcc tgcccctcct gcccctcctg cccctcctgc ccctcctcct
     9361 gctcctgccc ctcctcctgc tcctgcccct cctgctcctg cccctcccgc tcctgctcct
     9421 gctcctgttc caccgtgggt ccctttgcag ccaatgcaac ttggacgttt ttggggtctc
     9481 cggacaccat ctctatgtct tggccctgat cctgagccgc ccggggctcc tggtcttccg
     9541 cctcctcgtc ctcgtcctct tccccgtcct cgtccatggt tatcaccccc tcttctttga
     9601 ggtccactgc cgccggagcc ttctggtcca gatgtgtctc ccttctctcc taggccattt
     9661 ccaggtcctg tacctggccc ctcgtcagac atgattcaca ctaaaagaga tcaatagaca
     9721 tctttattag acgacgctca gtgaatacag ggagtgcaga ctcctgcccc ctccaacagc
     9781 ccccccaccc tcatcccctt catggtcgct gtcagacaga tccaggtctg aaaattcccc
     9841 atcctccgaa ccatcctcgt cctcatcacc aattactcgc agcccggaaa actcccgctg
     9901 aacatcctca agatttgcgt cctgagcctc aagccaggcc tcaaattcct cgtccccctt
     9961 tttgctggac ggtagggatg gggattctcg ggacccctcc tcttcctctt caaggtcacc
    10021 agacagagat gctactgggg caacggaaga aaagctgggt gcggcctgtg aggatcagct
    10081 tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc
    10141 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
    10201 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
    10261 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
    10321 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
    10381 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
    10441 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
    10501 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
    10561 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
    10621 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
    10681 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
    10741 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
    10801 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
    10861 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
    10921 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
    10981 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
    11041 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
    11101 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
    11161 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
    11221 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
    11281 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
    11341 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
    11401 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
    11461 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
    11521 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
    11581 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
    11641 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
    11701 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
    11761 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
    11821 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
    11881 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
    11941 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttgaag ctgtccctga
    12001 tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg
    12061 gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgaatt cgtcgacctc
    12121 gaaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
    12181 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
    12241 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
    12301 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
    12361 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
    12421 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
    12481 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
    12541 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
    12601 gttctcctct tcctcatctc cgggcctttc gacctgcatc catctagatc tcgagcagct
    12661 gaagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
    12721 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
    12781 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
    12841 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
    12901 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
    12961 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
    13021 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
    13081 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
    13141 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
    13201 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
    13261 cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca
    13321 tgcatcgatg atatcagatc cccgggatgc agaaattgat gatctattaa acaataaaga
    13381 tgtccactaa aatggaagtt tttcctgtca tactttgtta agaagggtga gaacagagta
    13441 cctacatttt gaatggaagg attggagcta cgggggtggg ggtggggtgg gattagataa
    13501 atgcctgctc tttactgaag gctctttact attgctttat gataatgttt catagttgga
    13561 tatcataatt taaacaagca aaaccaaatt aagggccagc tcattcctcc cactcatgat
    13621 ctatagatct atagatctct cgtgggatca ttgtttttct cttgattccc actttgtggt
    13681 tctaagtact gtggtttcca aatgtgtcag tttcatagcc tgaagaacga gatcagcagc
    13741 ctctgttcca catacacttc attctcagta ttgttttgcc aagttctaat tccatcagaa
    13801 gctggtcgac caattctcat gtttgacagc ttatcatcgc agatccgggc aacgttgttg
    13861 ccattgctgc aggcgcagaa ctggtaggta tggaagatct atacattgaa tcaatattgg
    13921 caattagcca tattagtcat tggttatata gcataaatca atattggcta ttggccattg
    13981 catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg
    14041 ccat
//
