LOCUS       pLV-EF1a-mCherry-MinD- 10253 bp ds-DNA     circular     30-JUN-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     source          1..10253
                     /label="source"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     LTR             1..634
                     /label="3' LTR"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    1303..1536
                     /label="RRE"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2028..2143
                     /label="cPPT/CTS"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        2221..2432
                     /label="EF-1-alpha core promoter"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(2296..2301)
                     /label="Kozak"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     intron          2433..3371
                     /label="EF-1-alpha intron A"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     3328..3348
                     /label="EF1a-F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          3328..3348
                     /label="EF1a-F"
                     /note="sequence: TCAAGCCTCAGACAGTGGTTC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          3356..3404
                     /label="mCherry-MinD F"
                     /note="sequence: TTTTCTTCCATTTCAGGTGTCGTGAGGATCCGCCACCatggtgagcaag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    3381..3386
                     /label="BamHI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(3381..3386)
                     /label="BamHI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     regulatory      3387..3392
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    3387..3392
                     /label="Kozak"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             3393..4100
                     /label="mCherry"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     complement(3538..3556)
                     /label="mCherry-R"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     3806..3825
                     /label="mCherry-F"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    4101..4133
                     /label="Linker"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    4134..4943
                     /label="MinD"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             4134..4943
                     /label="Translation 4134-4943"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLRNLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGVAKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGILASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVLRASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     primer          complement(4929..4977)
                     /label="snap-MinD R"
                     /note="sequence: TACCGCGGCCCGTCGACTCTAGAGCGGCCGCTTATCCCCCGAACAGCCG"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    4947..4954
                     /label="NotI"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(4947..4954)
                     /label="NotI"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(4998..5018)
                     /label="pLV-EF1a-R"
                     /note="sequence: TAGAGGGAAACCGTTGCTAGC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     complement(5191..5208)
                     /label="IRES reverse"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     5418..5437
                     /label="IRES-F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    5465..5470
                     /label="KpnI Site"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(5465..5470)
                     /label="KpnI Site"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     complement(5599..5618)
                     /label="Puro-R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             5599..6198
                     /label="PuroR"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     6095..6115
                     /label="Puro-F"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    6222..6810
                     /label="WPRE"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer_bind     complement(6275..6295)
                     /label="WPRE-R"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             complement(6693..6704)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    6946..6951
                     /label="KpnI Site"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(6946..6951)
                     /label="KpnI Site"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     LTR             7017..7650
                     /label="3' LTR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     complement(7774..7794)
                     /label="M13-rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     complement(7778..7794)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     complement(7778..7794)
                     /label="M13 Reverse"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(7791..7813)
                     /label="M13/pUC Reverse"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_binding    complement(7800..7822)
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    7802..7818
                     /label="lac operator"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     promoter        complement(7826..7856)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    7871..7892
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     complement(8009..8026)
                     /label="L4440"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     rep_origin      complement(8162..8844)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     rep_origin      complement(8180..8768)
                     /label="ori"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     complement(8260..8279)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(8939..9799)
                     /label="AmpR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             complement(8942..9601)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     9562..9581
                     /label="Amp-R"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        complement(9800..9904)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     polyA_signal    complement(9952..10073)
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     polyA_signal    9952..10086
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     complement(9989..10008)
                     /label="SV40pA-R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     10043..10062
                     /label="EBV-rev"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
ORIGIN
        1 TGGAAGGGCT AATTCACTCC CAAAGAAGAC AAGATATCCT TGATCTGTGG ATCTACCACA
       61 CACAAGGCTA CTTCCCTGAT TAGCAGAACT ACACACCAGG GCCAGGGGTC AGATATCCAC
      121 TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCC AGATAAGGTA GAAGAGGCCA
      181 ATAAAGGAGA GAACACCAGC TTGTTACACC CTGTGAGCCT GCATGGGATG GATGACCCGG
      241 AGAGAGAAGT GTTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC GTGGCCCGAG
      301 AGCTGCATCC GGAGTACTTC AAGAACTGCT GATATCGAGC TTGCTACAAG GGACTTTCCG
      361 CTGGGGACTT TCCAGGGAGG CGTGGCCTGG GCGGGACTGG GGAGTGGCGA GCCCTCAGAT
      421 CCTGCATATA AGCAGCTGCT TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA
      481 GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT
      541 TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC
      601 AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACTTGAAAG
      661 CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA GCGCGCACGG
      721 CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC GGAGGCTAGA
      781 AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA TCGCGATGGG
      841 AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT ATAGTATGGG
      901 CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT
      961 GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT
     1021 CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA
     1081 CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGACC ACCGCACAGC
     1141 AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT ATGAGGGACA ATTGGAGAAG
     1201 TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC
     1261 AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG
     1321 GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC
     1381 CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT TTGCTGAGGG CTATTGAGGC
     1441 GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT
     1501 GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA
     1561 ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA
     1621 GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT
     1681 AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC AAGAATTATT
     1741 GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC ATAACAAATT GGCTGTGGTA
     1801 TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT TTAAGAATAG TTTTTGCTGT
     1861 ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT
     1921 CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA GAAGAAGGTG GAGAGAGAGA
     1981 CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCTCGACGG TATCGCCTTT AAAAGAAAAG
     2041 GGGGGATTGG GGGGTACAGT GCAGGGGAAA GAATAGTAGA CATAATAGCA ACAGACATAC
     2101 AAACTAAAGA ATTACAAAAA CAAATTACAA AAATTCAAAA TTTTCGGGTT TATTACAGGG
     2161 ACAGCAGAGA TCCAGTTTAT CGATGAGGCC CTTTCGTCTT CACTCGAGGT GCCCGTCAGT
     2221 GGGCAGAGCG CACATCGCCC ACAGTCCCCG AGAAGTTGGG GGGAGGGGTC GGCAATTGAA
     2281 CCGGTGCCTA GAGAAGGTGG CGCGGGGTAA ACTGGGAAAG TGATGTCGTG TACTGGCTCC
     2341 GCCTTTTTCC CGAGGGTGGG GGAGAACCGT ATATAAGTGC AGTAGTCGCC GTGAACGTTC
     2401 TTTTTCGCAA CGGGTTTGCC GCCAGAACAC AGGTAAGTGC CGTGTGTGGT TCCCGCGGGC
     2461 CTGGCCTCTT TACGGGTTAT GGCCCTTGCG TGCCTTGAAT TACTTCCACC TGGCTGCAGT
     2521 ACGTGATTCT TGATCCCGAG CTTCGGGTTG GAAGTGGGTG GGAGAGTTCG AGGCCTTGCG
     2581 CTTAAGGAGC CCCTTCGCCT CGTGCTTGAG TTGAGGCCTG GCCTGGGCGC TGGGGCCGCC
     2641 GCGTGCGAAT CTGGTGGCAC CTTCGCGCCT GTCTCGCTGC TTTCGATAAG TCTCTAGCCA
     2701 TTTAAAATTT TTGATGACCT GCTGCGACGC TTTTTTTCTG GCAAGATAGT CTTGTAAATG
     2761 CGGGCCAAGA TCTGCACACT GGTATTTCGG TTTTTGGGGC CGCGGGCGGC GACGGGGCCC
     2821 GTGCGTCCCA GCGCACATGT TCGGCGAGGC GGGGCCTGCG AGCGCGGCCA CCGAGAATCG
     2881 GACGGGGGTA GTCTCAAGCT GGCCGGCCTG CTCTGGTGCC TGGCCTCGCG CCGCCGTGTA
     2941 TCGCCCCGCC CTGGGCGGCA AGGCTGGCCC GGTCGGCACC AGTTGCGTGA GCGGAAAGAT
     3001 GGCCGCTTCC CGGCCCTGCT GCAGGGAGCT CAAAATGGAG GACGCGGCGC TCGGGAGAGC
     3061 GGGCGGGTGA GTCACCCACA CAAAGGAAAA GGGCCTTTCC GTCCTCAGCC GTCGCTTCAT
     3121 GTGACTCCAC GGAGTACCGG GCGCCGTCCA GGCACCTCGA TTAGTTCTCG AGCTTTTGGA
     3181 GTACGTCGTC TTTAGGTTGG GGGGAGGGGT TTTATGCGAT GGAGTTTCCC CACACTGAGT
     3241 GGGTGGAGAC TGAAGTTAGG CCAGCTTGGC ACTTGATGTA ATTCTCCTTG GAATTTGCCC
     3301 TTTTTGAGTT TGGATCTTGG TTCATTCTCA AGCCTCAGAC AGTGGTTCAA AGTTTTTTTC
     3361 TTCCATTTCA GGTGTCGTGA GGATCCGCCA CCatggtgag caagggcgag gaggataaca
     3421 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
     3481 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     3541 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
     3601 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
     3661 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
     3721 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
     3781 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     3841 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc
     3901 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga
     3961 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca
     4021 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
     4081 gcatggacga gctgtacaag GACAGCGCCG GAAGTGCTGG CTCTGCGGGT ACCATGGCCC
     4141 GCATAATAGT TGTGACTTCA GGTAAGGGCG GAGTTGGAAA AACCACATCC AGCGCAGCCA
     4201 TAGCTACCGG CCTCGCACAG AAAGGCAAGA AAACTGTGGT CATAGATTTT GATATTGGGC
     4261 TCAGGAACCT GGACCTGATA ATGGGCTGCG AACGGCGCGT TGTCTACGAT TTTGTCAATG
     4321 TCATCCAAGG CGACGCGACT CTTAATCAGG CACTTATAAA AGATAAACGA ACCGAAAACT
     4381 TGTATATTCT TCCTGCCTCA CAAACGCGGG ACAAAGACGC GCTCACCAGG GAGGGTGTCG
     4441 CAAAAGTTTT GGATGACTTG AAAGCGATGG ACTTTGAGTT CATAGTTTGT GACTCTCCCG
     4501 CCGGTATAGA GACAGGGGCA CTTATGGCAC TGTACTTCGC AGATGAAGCC ATAATCACAA
     4561 CTAATCCGGA GGTGTCTAGT GTGCGAGATT CAGACCGAAT ACTGGGGATA CTCGCCAGCA
     4621 AATCACGGAG GGCAGAAAAC GGGGAGGAGC CAATTAAGGA GCATCTCCTG CTTACCCGAT
     4681 ATAACCCCGG TCGAGTATCT CGGGGAGATA TGCTTTCAAT GGAAGATGTC CTCGAGATTC
     4741 TGAGGATAAA ACTGGTAGGC GTTATCCCGG AAGACCAGTC CGTCCTCCGC GCATCTAATC
     4801 AGGGGGAGCC TGTCATACTT GACATAAATG CGGATGCTGG GAAAGCTTAT GCCGATACGG
     4861 TAGAACGGCT CCTCGGGGAA GAACGGCCCT TCCGCTTCAT AGAGGAGGAA AAGAAGGGAT
     4921 TCCTTAAACG GCTGTTCGGG GGATAAGCGG CCGCTCTAGA GTCGACGGGC CGCGGTAACA
     4981 ATTGTTAACT AACTTAAGCT AGCAACGGTT TCCCTCTAGC GGGATCAATT CCGCCCCCCC
     5041 CCCCTAACGT TACTGGCCGA AGCCGCTTGG AATAAGGCCG GTGTGCGTTT GTCTATATGT
     5101 TATTTTCCAC CATATTGCCG TCTTTTGGCA ATGTGAGGGC CCGGAAACCT GGCCCTGTCT
     5161 TCTTGACGAG CATTCCTAGG GGTCTTTCCC CTCTCGCCAA AGGAATGCAA GGTCTGTTGA
     5221 ATGTCGTGAA GGAAGCAGTT CCTCTGGAAG CTTCTTGAAG ACAAACAACG TCTGTAGCGA
     5281 CCCTTTGCAG GCAGCGGAAC CCCCCACCTG GCGACAGGTG CCTCTGCGGC CAAAAGCCAC
     5341 GTGTATAAGA TACACCTGCA AAGGCGGCAC AACCCCAGTG CCACGTTGTG AGTTGGATAG
     5401 TTGTGGAAAG AGTCAAATGG CTCTCCTCAA GCGTATTCAA CAAGGGGCTG AAGGATGCCC
     5461 AGAAGGTACC CCATTGTATG GGATCTGATC TGGGGCCTCG GTGCACATGC TTTACATGTG
     5521 TTTAGTCGAG GTTAAAAAAA CGTCTAGGCC CCCCGAACCA CGGGGACGTG GTTTTCCTTT
     5581 GAAAAACACG ATAATACCAT GACCGAGTAC AAGCCCACGG TGCGCCTCGC CACCCGCGAC
     5641 GACGTCCCCA GGGCCGTACG CACCCTCGCC GCCGCGTTCG CCGACTACCC CGCCACGCGC
     5701 CACACCGTCG ATCCGGACCG CCACATCGAG CGGGTCACCG AGCTGCAAGA ACTCTTCCTC
     5761 ACGCGCGTCG GGCTCGACAT CGGCAAGGTG TGGGTCGCGG ACGACGGCGC CGCGGTGGCG
     5821 GTCTGGACCA CGCCGGAGAG CGTCGAAGCG GGGGCGGTGT TCGCCGAGAT CGGCCCGCGC
     5881 ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC GCGCAGCAAC AGATGGAAGG CCTCCTGGCG
     5941 CCGCACCGGC CCAAGGAGCC CGCGTGGTTC CTGGCCACCG TCGGCGTCTC GCCCGACCAC
     6001 CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG CTCCCCGGAG TGGAGGCGGC CGAGCGCGCC
     6061 GGGGTGCCCG CCTTCCTGGA GACCTCCGCG CCCCGCAACC TCCCCTTCTA CGAGCGGCTC
     6121 GGCTTCACCG TCACCGCCGA CGTCGAGGTG CCCGAAGGAC CGCGCACCTG GTGCATGACC
     6181 CGCAAGCCCG GTGCCTGACC CGGGCGGGGC GCGTCTGGAA CAATCAACCT CTGGATTACA
     6241 AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT
     6301 ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT
     6361 CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC
     6421 GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA
     6481 CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA
     6541 TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG
     6601 TGGTGTTGTC GGGGAAGCTG ACGTCCTTTC CATGGCTGCT CGCCTGTGTT GCCACCTGGA
     6661 TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT
     6721 CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA
     6781 GTCGGATCTC CCTTTGGGCC GCCTCCCCGC CTGGAATTAA TTCTGCAGTC GAGACCTAGA
     6841 AAAACATGGA GCAATCACAA GTAGCAATAC AGCAGCTACC AATGCTGATT GTGCCTGGCT
     6901 AGAAGCACAA GAGGAGGAGG AGGTGGGTTT TCCAGTCACA CCTCAGGTAC CTTTAAGACC
     6961 AATGACTTAC AAGGCAGCTG TAGATCTTAG CCACTTTTTA AAAGAAAAGA GGGGACTGGA
     7021 AGGGCTAATT CACTCCCAAC GAAGACAAGA TATCCTTGAT CTGTGGATCT ACCACACACA
     7081 AGGCTACTTC CCTGATTAGC AGAACTACAC ACCAGGGCCA GGGGTCAGAT ATCCACTGAC
     7141 CTTTGGATGG TGCTACAAGC TAGTACCAGT TGAGCCAGAT AAGGTAGAAG AGGCCAATAA
     7201 AGGAGAGAAC ACCAGCTTGT TACACCCTGT GAGCCTGCAT GGGATGGATG ACCCGGAGAG
     7261 AGAAGTGTTA GAGTGGAGGT TTGACAGCCG CCTAGCATTT CATCACGTGG CCCGAGAGCT
     7321 GCATCCGGAG TACTTCAAGA ACTGCTGATA TCGAGCTTGC TACAAGGGAC TTTCCGCTGG
     7381 GGACTTTCCA GGGAGGCGTG GCCTGGGCGG GACTGGGGAG TGGCGAGCCC TCAGATCCTG
     7441 CATATAAGCA GCTGCTTTTT GCCTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT
     7501 GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG
     7561 TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC
     7621 CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GTAGTAGTTC ATGTCATCTT ATTATTCAGT
     7681 ATTTATAACT TGCAAAGAAA TGAATATCAG AGAGTGAGAG GCCTTGACAT TGCTAGCGTT
     7741 TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA
     7801 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT
     7861 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC
     7921 AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG
     7981 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC
     8041 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG
     8101 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA
     8161 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC
     8221 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC
     8281 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG
     8341 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT
     8401 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC
     8461 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC
     8521 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG
     8581 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG
     8641 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA
     8701 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG
     8761 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT
     8821 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA
     8881 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT
     8941 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG
     9001 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA
     9061 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC
     9121 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT
     9181 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG
     9241 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA
     9301 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG
     9361 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA
     9421 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG
     9481 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT
     9541 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA
     9601 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA
     9661 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG
     9721 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC
     9781 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT
     9841 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC
     9901 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCGACGG ATCGGGAGAT CAACTTGTTT
     9961 ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA
    10021 TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC
    10081 TGGATCAACT GGATAACTCA AGCTAACCAA AATCATCCCA AACTTCCCAC CCCATACCCT
    10141 ATTACCACTG CCAATTACCT GTGGTTTCAT TTACTCTAAA CCTGTGATTC CTCTGAATTA
    10201 TTTTCATTTT AAAGAAATTG TATTTGTTAA ATATGTACTA CAAACTTAGT AGT
//