LOCUS       pAR216_RoRH        5267 bp DNA     circular SYN 28-NOV-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 28, 2023 from SnapGene Viewer 7.0.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    250..298
                     /label=T5 H207 promoter
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             353..1447
                     /codon_start=1
                     /label=NmPAL
                     /note="color: #993366"
                     /translation="MKVNRPAERASFGSFVLDAGSARFVGSDELALVLGFAPGDVVLTP
                     AVVLAHLHPDDRLEWQAGLQRCLATGRPVVVNHLLLTAEAEPRPAMTTLTALTEQDRVR
                     AVTGVITDLSDRVRRATEAEIRQAVRAAAATRSEIDQAKGIVMAAFDVDADQAFALLKW
                     HSSQSNRKLRDLATGMIEGLAAANSALPLRRRLSTVFTDMGCPAPSTKGWTVPVTDIGL
                     PPTSGLIPTALLPGILTRAAHDASVAITVADVTAPDQPLVYANPAFERLTGYAAAEVLG
                     RNCRFLQAESGDPHERSAIRSAIANGDAVTTLIRNFRQDGHAFWNEFHLSPVRNGAGRV
                     THYIGYQLDVTERVERDQQLEQLAG"
     CDS             1448..1477
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /note="color: #cc99b2"
                     /translation="EQKLISEEDL"
     terminator      1544..1591
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    1698..1746
                     /label=PL Lambda promoter
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1777..1850
                     /label=RoRH cis-RNA
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             1863..1880
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     CDS             1890..1907
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /note="color: #cc99b2
                     Cleavage site after base 1901"
                     /translation="LVPRGS"
     misc_feature    1908..1960
                     /label=multiple-cloning site
                     /note="color: #ff00ff"
     terminator      2087..2173
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
                     /note="color: #ffffff; direction: RIGHT"
     terminator      2265..2292
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             complement(2494..3285)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /note="color: #ccffcc"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(3286..3377)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      complement(3425..4161)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
                     /note="color: #ffff00"
     CDS             complement(4363..5241)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /note="color: #993366"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctt tttaaaaaat tcatttgcta aacgcttcaa attctcgtat aattactttt
      301 ctccataccc gttttttggg ctaacccgcc gaaacctaag gaaatacata gtatgaaagt
      361 taatcgtccg gcagaacgtg ccagctttgg tagctttgtt ctggatgcag gtagcgcacg
      421 ttttgttggt agtgatgaac tggcactggt tctgggtttt gcaccgggtg atgttgttct
      481 gacaccggca gttgttctgg cacatctgca tcctgatgat cgtctggaat ggcaggcagg
      541 tctgcagcgt tgtctggcaa ccggtcgtcc ggtggttgtt aatcatctgc tgctgaccgc
      601 agaagcagaa ccgcgtccgg caatgacaac cctgaccgca ctgaccgaac aggatcgtgt
      661 gcgtgcagtt accggtgtta ttaccgatct gagcgatcgt gttcgtcgtg caaccgaagc
      721 agaaattcgt caggcagttc gtgcagcagc agcaacccgt agcgaaattg atcaggcaaa
      781 aggtattgtt atggcagcct ttgatgttga tgcagatcag gcatttgcac tgctgaaatg
      841 gcatagcagc cagagcaatc gtaaactgcg tgatctggcc accggtatga ttgaaggtct
      901 ggcagcagcc aatagcgcac tgccgctgcg tcgtcgtctg agcaccgttt ttaccgatat
      961 gggttgtccg gcaccgagca ccaaaggttg gaccgttccg gtgaccgata ttggtctgcc
     1021 tccgaccagc ggtctgattc cgacagcact gctgcctggt attctgaccc gtgcagcaca
     1081 tgatgcaagc gttgcaatta ccgttgcaga tgttaccgca ccggatcagc ctctggttta
     1141 tgcaaatcct gcatttgaac gtctgaccgg ttatgcagca gccgaagttc tgggtcgtaa
     1201 ttgtcgtttt ctgcaggcag aaagcggtga tccgcatgaa cgtagcgcaa ttcgtagcgc
     1261 cattgcaaat ggtgatgcag tgaccacact gattcgtaat tttcgtcagg atggtcatgc
     1321 cttttggaac gaatttcatc tgagtccggt tcgtaatggt gcaggtcgtg ttacccatta
     1381 cattggttat cagctggatg tgaccgaacg tgttgaacgt gatcagcagc tggaacagct
     1441 ggcaggcgaa cagaaactga tttctgaaga agatctgtaa gatccggctg ctaacaaagc
     1501 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
     1561 ggcctctaaa cgggtcttga ggggtttttt gctcggccgc actcgagagc ttggctgttt
     1621 tggcggatga gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct
     1681 gataaaacag aatttgccgg tgttgacata aataccactg gcggtgatac tgagcacatc
     1741 agcaggcccc tctagaaata attttgttta actttaaata gatcacctgc tagccggttc
     1801 agcagcgagc cgtaagcctc actgccggaa ctgataggag gagatatacc atgggcagca
     1861 gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat atgggatccg
     1921 aattcgagct ccgtcgacaa gcttgcggcc gcactcgagc atgagatccc tggcggcagt
     1981 agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat
     2041 ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa
     2101 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct
     2161 gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg
     2221 gcgggcagga cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac
     2281 ggatggcctt tttgcgtttc tacaaactct ttttgtttat ttttctaaat acattcaaat
     2341 atgtatccgc tcatggacac tgcttccggt agtcaataaa ccggtaaacc agcaatagac
     2401 ataagcggct atttaacgac cctgccctga accgacgacc gggtcatcgt ggccggatct
     2461 tgcggcccct cggcttgaac gaattgttag acattatttg ccgactacct tggtgatctc
     2521 gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc
     2581 ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag
     2641 gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct
     2701 gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg
     2761 cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg
     2821 atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt
     2881 cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc
     2941 attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat
     3001 gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga
     3061 agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac
     3121 ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga
     3181 gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac
     3241 ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcatactct tcctttttca
     3301 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     3361 ttagaaaaat aaacaaatag ctagctcact cggtcgctac gctccgggcg tgagactgcg
     3421 gcgggcgctg cggacacata caaagttacc cacagattcc gtggataagc aggggactaa
     3481 catgtgaggc aaaacagcag ggccgcgccg gtggcgtttt tccataggct ccgccctcct
     3541 gccagagttc acataaacag acgcttttcc ggtgcatctg tgggagccgt gaggctcaac
     3601 catgaatctg acagtacggg cgaaacccga caggacttaa agatccccac cgtttccggc
     3661 gggtcgctcc ctcttgcgct ctcctgttcc gaccctgccg tttaccggat acctgttccg
     3721 cctttctccc ttacgggaag tgtggcgctt tctcatagct cacacactgg tatctcggct
     3781 cggtgtaggt cgttcgctcc aagctgggct gtaagcaaga actccccgtt cagcccgact
     3841 gctgccttat ccggtaactg ttcacttgag tccaacccgg aaaagcacgg taaaacgcca
     3901 ctggcagcag ccattggtaa ctgggagttc gcagaggatt tgtttagcta aacacgcggt
     3961 tgctcttgaa gtgtgcgcca aagtccggct acactggaag gacagatttg gttgctgtgc
     4021 tctgcgaaag ccagttacca cggttaagca gttccccaac tgacttaacc ttcgatcaaa
     4081 ccacctcccc aggtggtttt ttcgtttaca gggcaaaaga ttacgcgcag aaaaaaagga
     4141 tctcaagaag atcctttgat cttttctact gaaccgctct agatttcagt gcaatttatc
     4201 tcttcaaatg tagcacctga agtcagcccc atacgatata agttgtaatt ctcatgttag
     4261 tcatgccccg catgcataat gtgcctgtca aatggacgaa gcagggattc tgcaaaccct
     4321 atgctactcc gtcaagccgt caattgtctg attcgttacc aattatgaca acttgacggc
     4381 tacatcattc actttttctt cacaaccggc acggaactcg ctcgggctgg ccccggtgca
     4441 ttttttaaat acccgcgaga aatagagttg atcgtcaaaa ccaacattgc gaccgacggt
     4501 ggcgataggc atccgggtgg tgctcaaaag cagcttcgcc tggctgatac gttggtcctc
     4561 gcgccagctt aagacgctaa tccctaactg ctggcggaaa agatgtgaca gacgcgacgg
     4621 cgacaagcaa acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc
     4681 gctgatgtac tgacaagcct cgcgtacccg attatccatc ggtggatgga gcgactcgtt
     4741 aatcgcttcc atgcgccgca gtaacaattg ctcaagcaga tttatcgcca gcagctccga
     4801 atagcgccct tccccttgcc cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg
     4861 ctggtgcgct tcatccgggc gaaagaaccc cgtattggca aatattgacg gccagttaag
     4921 ccattcatgc cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc attcgcgagc
     4981 ctccggatga cgaccgtagt gatgaatctc tcctggcggg aacagcaaaa tatcacccgg
     5041 tcggcaaaca aattctcgtc cctgattttt caccaccccc tgaccgcgaa tggtgagatt
     5101 gagaatataa cctttcattc ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc
     5161 ctcaatcggc gttaaacccg ccaccagatg ggcattaaac gagtatcccg gcagcagggg
     5221 atcattttgc gcttcagcca tacttttcat actcccgcca ttcagag
//
