LOCUS       pST                     7054 bp ds-DNA     circular SYN 06-DEC-2023
DEFINITION  synthetic circular DNA.
ACCESSION   . (modified)
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 26, 2018 from SnapGene 4.2.6 http://www.snapgene.com
FEATURES             Location/Qualifiers
     misc_feature    join(<7025..7054,1..25)
                     /label="L3S2P21 term"
     regulatory      112..1194
                     /note="/label=lacI"
     misc_feature    1125
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     regulatory      1228..1827
                     /note="/label=TetR"
     gene            complement(3037..3852)
                     /label="KanR2"
     misc_feature    3870
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     promoter        3975..4003
                     /label="tac_promoter"
     misc_feature    4103..4139
                     /label="RBS Q2"
     Protein         4140..4919
                     /created_by="User"
                     /modified_by="User"
                     /label="TtgV TF"
     misc_feature    4920..4973
                     /label="Terminator"
     misc_feature    4978..>5007
                     /label="Promoter 2"
     promoter        5008..5056
                     /created_by="User"
                     /modified_by="User"
                     /label="Binding Sequence"
     RBS             5076..5084
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to
                     aregion in the 3' end of the 16S rRNA (Chen et al., 1994)"
                     /label="Shine-Dalgarno sequence"
     Protein         5090..5806
                     /created_by="User"
                     /Color="#00ee77"
                     /modified_by="User"
                     /label="SFGFP"
     misc_feature    5094..5803
                     /Original_Bases="TGCGTAAAGGTGAAGAACTGTTCACCGGTGTTGTTCCGATC
                     CTGGTTGAACTGGACGGTGACGTTAACGGTCACAAATTCTCTGTTCGTGGTGAAGGTG
                     AAGGTGACGCTACCAACGGTAAACTGACCCTGAAATTCATCTGCACCACCGGTAAACT
                     GCCGGTTCCGTGGCCGACCCTGGTTACCACCCTGACCTACGGTGTTCAGTGCTTCGCT
                     CGTTACCCGGACCACATGAAACAGCACGACTTCTTCAAATCTGCTATGCCGGAAGGTT
                     ACGTTCAGGAACGTACCATCTCTTTCAAAGACGACGGTACCTACAAAACCCGTGCTGA
                     AGTTAAATTCGAAGGTGACACCCTGGTTAACCGTATCGAACTGAAAGGTATCGACTTC
                     AAAGAAGACGGTAACATCCTGGGTCACAAACTGGAATACAACTTCAACTCTCACAACG
                     TTTACATCACCGCTGACAAACAGAAAAACGGTATCAAAGCTAACTTCAAAATCCGTCA
                     CAACGTTGAAGACGGTTCTGTTCAGCTGGCTGACCACTACCAGCAGAACACCCCGATC
                     GGTGACGGTCCGGTTCTGCTGCCGGACAACCACTACCTGTCTACCCAGTCTGTTCTGT
                     CTAAAGACCCGAACGAAAAACGTGACCACATGGTTCTGCTGGAATTCGTTACCGCTGC
                     TGGTATCACCCACGGTATGGACGAACTGTACAAA"
                     /label="TGCGTAAAGGTGAAGAACTGTTCACCGGTGTTGTTCCGATCCTGGTTGAA
                     CTGGACGGTGACGTTAACGGTCACAAATTCTCTGTTCGTGGTGAAGGTGAAGGTGACG
                     CTACCAACGGTAAACTGACCCTGAAATTCATCTGCACCACCGGTAAACTGCCGGTTCC
                     GTGGCCGACCCTGGTTACCACCCTGACCTACGGTGTTCAGTGCTTCGCTCGTTACCCG
                     GACCACATGAAACAGCACGACTTCTTCAAATCTGCTATGCCGGAAGGTTACGTTCAGG
                     AACGTACCATCTCTTTCAAAGACGACGGTACCTACAAAACCCGTGCTGAAGTTAAATT
                     CGAAGGTGACACCCTGGTTAACCGTATCGAACTGAAAGGTATCGACTTCAAAGAAGAC
                     GGTAACATCCTGGGTCACAAACTGGAATACAACTTCAACTCTCACAACGTTTACATCA
                     CCGCTGACAAACAGAAAAACGGTATCAAAGCTAACTTCAAAATCCGTCACAACGTTGA
                     AGACGGTTCTGTTCAGCTGGCTGACCACTACCAGCAGAACACCCCGATCGGTGACGGT
                     CCGGTTCTGCTGCCGGACAACCACTACCTGTCTACCCAGTCTGTTCTGTCTAAAGACC
                     CGAACGAAAAACGTGACCACATGGTTCTGCTGGAATTCGTTACCGCTGCTGGTATCAC
                     CCACGGTATGGACGAACTGTACAAA"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    5813..5824
                     /label="B0064 RBS"
     gene            5831..7024
                     /label="tetA"
ORIGIN      
        1 ggggggcctt ttttcgtttt ggtccaatgg cggcgcgcca tcgaatggcg caaaaccttt
       61 cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa tatgaaacca
      121 gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg
      181 gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg
      241 gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg
      301 attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt
      361 aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc
      421 gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc
      481 attaactatc cgctggatga ccaggatgcc attgctgtgg aagctacctg cactaatgtt
      541 ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat
      601 gaggacggta tgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg
      661 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa
      721 tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg
      781 tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg
      841 gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc
      901 gttggtgcgg atatctcggt agtgggatac gacgataccg aagatagctc atgttatatc
      961 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc
     1021 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc agtctcactg
     1081 gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctccccccg cgcgttggcc
     1141 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgataatcc
     1201 aggaggaaaa aaatgtccag attagataaa agtaaagtga ttaacagcgc attagagctg
     1261 cttaatgagg tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta
     1321 gagcagccta cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc
     1381 attgagatgt tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa
     1441 gattttttac gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga
     1501 gcaaaagtac atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa
     1561 ttagcctttt tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct
     1621 gtggggcatt ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa
     1681 gaaagggaaa cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta
     1741 tttgatcacc aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga
     1801 ttagaaaaac aacttaaatg tgaaagtggg tcctaataat tggtaacgaa tcagacaatt
     1861 gacggctcga gggagtagca tagggtttgc agaatccctg cttcgtccat ttgacaggca
     1921 cattatgcat cgatgataag ctgtcaaaca tgagcagatc ctctacgccg gacgcatcgt
     1981 ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga
     2041 tggggaagat cgggctcgcc acttcgggct catgagcaaa tgttttatct gaggtgcttc
     2101 ctcgctcact gactcgctgc acgaggcaga cctcagcgct agcggagtgt atactggctt
     2161 actatgttgg cactgatgag ggtgtcagtg aagtgcttca tgtggcagga gaaaaaaggc
     2221 tgcaccggtg cgtcagcaga atatgtgata caggatatat tccgcttcct cgctcactga
     2281 ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga
     2341 tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg
     2401 tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg
     2461 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg
     2521 cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg
     2581 tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat
     2641 gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     2701 caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg
     2761 agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact
     2821 gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa
     2881 aaccgccctg caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac
     2941 gatctcaaga agatcatctt attaaggggt ctgacgctca gtggaacgaa aaatcaatct
     3001 aaagtatata tgagtaaact tggtctgaca gttaccttag aaaaactcat cgagcatcaa
     3061 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
     3121 ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg
     3181 gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat
     3241 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag
     3301 cttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc
     3361 actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg
     3421 atcgctatta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc
     3481 cagcgcatca acaatgtttt cacctgaatc aggatattct tctaatacct ggaatgctgt
     3541 tttcccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt
     3601 gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac
     3661 atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc
     3721 atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc
     3781 atataaatca gcatccatgt tggaatttaa tcgcggcctg gagcaagacg tttcccgttg
     3841 aatatggctc ataacacccc ttgtattacc gtttatgtaa gcagacagtt ttattgttca
     3901 tgatgatata tttttatctt gtgcaatgta catcagagat tttgagacac aaggagaacg
     3961 atcgttggct gtgttgacaa ttaatcatcg gctcgtataa tgtgtggaat tgtgagcgct
     4021 cacaattagt cgtcaagtgc tgtgcttgca cttctgatga ggcagtgatg ccgaaacgac
     4081 ctctacaaat aattttgttt aagccatgtc attggctttt gatagaggac aactactaga
     4141 tgaatcaatc ggacgagaac atcggtaaag cgggtggtat tcaagttatt gcacgtgcgg
     4201 cgtctattat gcgtgccctc ggttcccacc cccatggtct ttctctggcc gcaatcgccc
     4261 agcttgtggg tttgccgcgt agtacggtgc aacgtattat taatgcgtta gaagaggaat
     4321 ttcttgtgga agccctgggc ccggcaggcg gcttccgtct gggaccggcc ctgggtcaac
     4381 tcattaacca agctcagact gatatcctga gcctcgttaa gccatattta cgttccttgg
     4441 ccgaagaatt agatgaatcc gtttgcttag ccagtttggc tggtgacaag atttatgttt
     4501 tagatcgcat tgtctcggaa cgtgaattgc gtgtcgtttt cccaatcggc atcaatgttc
     4561 cggcggcggc caccgccgcg ggtaaggttt tgctggccgc gctgccagat gagactctcc
     4621 aggcagcctt aggtgaacaa ttgcccgttc tgacatcaaa tacgctgggg cgtaaagctt
     4681 tggtaaagca gctcagtgag gtccgccaat ccggagtggc atcagacctt gatgaacata
     4741 tcgacggtgt ctgtagtttt gcgaccctgc tggacacgta tctgggttac tatagcctgg
     4801 caattgttat gccttccagc cgtgcttcga aacaaagcga tctcattaag aaggcacttt
     4861 tacaatcgaa acttaacatt gaacgcgcga ttggtcgtgc cagcaagaag gccccgtgag
     4921 tcagtttcac ctgttttacg taaaaacccg cttcggcggg tttttacttt tggtactggt
     4981 atggaagcta tacgttacca attgacaaaa aaccacatag tgatacacta ttctgcaatg
     5041 cgggccatgc attgtgtcac acaggaaaca tgacctaagg aggtaaataa tgagaaaggg
     5101 agaggagttg tttactggcg tcgtgcccat tttagtcgag ttagatggag atgtaaatgg
     5161 gcataagttt agtgtacggg gcgagggaga gggcgatgcc acaaatggaa agcttacttt
     5221 aaagtttatt tgtacaacgg ggaagttacc agtaccttgg cctacacttg ttacaacttt
     5281 aacgtatgga gtccaatgtt ttgcacggta tccagatcat atgaagcaac atgatttctt
     5341 taagtcggcc atgccagagg gatatgtcca agagcgcaca ataagcttta aggatgatgg
     5401 aacatataag acacgggcag aggtgaagtt tgagggagat acacttgtaa atcgcataga
     5461 gttgaagggc attgatttta aggaagatgg caacattctg gggcataagt tagagtataa
     5521 ctttaacagc cacaatgtat atattacggc ggacaagcaa aagaatggga taaaggccaa
     5581 ttttaagatt cgccataatg tcgaggatgg gtccgtccaa ctcgccgatc attatcaaca
     5641 aaatactccc attggcgatg gcccagtctt gctccctgat aatcattatc tctcaactca
     5701 atcagtactc tcaaaggatc ctaatgagaa gcgggatcat atggtccttt tagagtttgt
     5761 cactgcagcc ggcattacac atggcatgga tgagttgtat aagtaactcg gtaaagaggg
     5821 gaaatactag atgatgaaat ctaacaatgc gctcatcgtc atcctcggca ccgtcaccct
     5881 ggatgctgta ggcataggct tggttatgcc ggtactgccg ggcctcttgc gggatatcgt
     5941 ccattccgac agcatcgcca gtcactatgg cgtgctgcta gcgctatatg cgttgatgca
     6001 atttctatgc gcacccgttc tcggagcact gtccgaccgc tttggccgcc gcccagtcct
     6061 gctcgcttcg ctacttggag ccactatcga ctacgcgatc atggcgacca cacccgtcct
     6121 gtggatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc
     6181 tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat
     6241 gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc
     6301 catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact
     6361 gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaccgatgc ccttgagagc
     6421 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat
     6481 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt
     6541 cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg
     6601 aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac gtttcggcga
     6661 gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt
     6721 cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg
     6781 gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct
     6841 tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc attggaccgc tgatcgtcac
     6901 ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg taggcgccgc
     6961 cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac
     7021 ctgaaccaaa ttccagaaaa gaggcctccc gaaa
//
