LOCUS       pCDF-B-pC        6170 bp DNA     circular SYN 05-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 24, 2024 from SnapGene 6.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6170
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..28
                     /label=SDB1a
     misc_feature    347..399
                     /label=TT_L3S3P21
     misc_feature    425..479
                     /label=Prpa
     misc_feature    480..496
                     /label=cI*-O5G6GP
                     /note="cI operator"
     misc_feature    497..574
                     /label=ribozyme_elvJ
                     /note="Molecular insulator between Promoters and genes."
     misc_feature    581..591
                     /label=RBS_B0033
                     /label=nonstandard type: B0033
     misc_feature    598..1302
                     /label=cinI_LVA
                     /note="cinI gene. Express the enzyme that synthesize OHC14"
     CDS             598..1302
                     /codon_start=1
                     /label=cinI_LVA
                     /translation="MFVIIQAHEYQKYAAVLDQMFRLRKKVFADTLCWDVPVIGPYERD
                     SYDSLAPAYLVWCNDSRTRLYGGMRLMPTTGPTLLYDVFRETFPDAADLIAPGIWEGTR
                     MCIDEEAIAKDFPEIDAGRAFSMMLLALCECALDHGIHTMISNYEPYLKRVYKRAGAEV
                     EELGRADGYGKYPVCCGAFEVSDRVLRKMRAALGLTLPLYVRHVPARSVVTQFLEMAAT
                     SAANDENYALVA"
     primer_bind     657..676
                     /label=SDB1b
     misc_feature    1267..1299
                     /label=degtag_LVA
     misc_feature    1315..1375
                     /label=TT_L3S2P21
     misc_feature    1376..1607
                     /label=Pcin
     primer_bind     1466..1487
                     /label=SDB1c
     misc_feature    1608..1682
                     /label=ribozyme_riboJ
                     /note="Molecular insulator between promoter and gene"
     RBS             1690..1701
                     /label=B0064
                     /label=RBS B0031 (0.07)
     misc_feature    1708..2832
                     /label=lacI_LVA
     CDS             1708..2832
                     /codon_start=1
                     /label=lacI_LVA
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQRPAANDENYALVA*"
     primer_bind     2266..2286
                     /label=SDB1d
     misc_feature    2788..2826
                     /label=degtag_LVA
     primer_bind     complement(2815..2832)
                     /label=DB4-rv
     RBS             2846..2857
                     /label=B0064
     misc_feature    2867..3619
                     /label=cI_LVA
     CDS             2867..3619
                     /codon_start=1
                     /label=cI_LVA
                     /translation="STKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQS
                     GVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEYP
                     VFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPDG
                     MLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSVV
                     GKVIASQWPEETFGRPAANDENYALVA*"
     primer_bind     3092..3109
                     /label=SDB1e
     misc_feature    3575..3613
                     /label=degtag_LVA
     primer_bind     complement(3602..3626)
                     /label=DB4-rv
     misc_feature    3627..3638
                     /label=RBS B0030 (0.6)
     misc_feature    3645..4403
                     /label=sfGFP_ASV
     CDS             3645..4403
                     /codon_start=1
                     /transl_table=11
                     /label=sfGFP_ASV
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYKRPAANDENYAASV*"
     primer_bind     3923..3941
                     /label=SDB1f
     misc_feature    4359..4397
                     /label=degtag_ASV
     primer_bind     4386..4410
                     /label=DB4-fw
     misc_feature    4413..4499
                     /label=TT_rrnB1
     misc_feature    complement(4662..5267)
                     /label=ori
     primer_bind     4837..4857
                     /label=SDB1g
     primer_bind     4893..4910
                     /label=SDB6-6
     misc_feature    join(5605..6170,1..223)
                     /label=Spec
                     /note="Spec resistance"
     primer_bind     5758..5775
                     /label=SDB1h
ORIGIN
        1 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
       61 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
      121 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
      181 tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc taacaattcg
      241 ttcaagccga ggggccgcaa gatccggcca cgatgacccg gtcgtcggtt cagggcaggg
      301 tcgttaaata gccgcttatg tctattgctg gtttaccggt agatctccaa ttattgaagg
      361 cctccctaac ggggggcctt tttttgtttc tggtctcccg cttaacgatc gttggctggt
      421 cgacacctgt ccgatcggac agttttacgc aagaaaatgg tttgttactt tcgaataaat
      481 atcggcgccg ccgataagcc ccatagggtg gtgtgtacca cccctgatga gtccaaaagg
      541 acgaaatggg gcctctacaa ataattttgt ttaaggatcc tcacacagga cgaattcatg
      601 ttcgttatca ttcaggcaca tgagtatcag aaatacgctg ccgtactcga ccagatgttt
      661 cgtctgcgca agaaggtctt cgccgatacg ctctgctggg acgttcctgt catcggccct
      721 tacgaacgtg acagctacga ttcgcttgct cccgcctatc tcgtctggtg caacgacagc
      781 cgcacccgtc tttatggcgg catgcgcctg atgccgacga ccggcccgac ccttctctac
      841 gacgtcttcc gcgagacgtt ccctgatgcc gccgatctta tcgcccccgg catctgggaa
      901 ggcacgcgca tgtgcatcga cgaggaggcg atcgccaagg atttccccga gatcgacgcc
      961 ggccgcgcct tctccatgat gctgctcgcg ctttgcgaat gcgcgctcga tcacggcatc
     1021 cacacgatga tctccaacta cgagccctac ctcaagcgcg tctacaagcg cgccggcgcc
     1081 gaggtggaag aactcggccg cgcagacggc tacggcaaat atcccgtctg ctgcggcgcc
     1141 ttcgaagtct cggaccgcgt gctgcgcaag atgcgcgccg ccctcggcct caccctaccc
     1201 ctttatgtca ggcacgtgcc ggcccgctcg gtcgtgaccc aattcctgga gatggcagca
     1261 actagtgctg caaacgacga aaactacgct ttagtagctt gaactagttg ataactcggt
     1321 accaaattcc agaaaagagg cctcccgaaa ggggggcctt ttttcgtttt ggtccccctt
     1381 tgtgcgtcca aacggacgca cggcgctcta aagcgggtcg cgatctttca gattcgctcc
     1441 tcgcgctttc agtctttgtt ttggcgcatg tcgttatcgc aaaaccgctg cacacttttg
     1501 cgcgacatgc tctgatcccc ctcatctggg ggggcctatc tgagggaatt tccgatccgg
     1561 ctcgcctgaa ccattctgct ttccacgaac ttgaaaacgc tacgcgtagc tgtcaccgga
     1621 tgtgctttcc ggtctgatga gtccgtgagg acgaaacagc ctctacaaat aattttgttt
     1681 aatctagaga aagaggggaa atactagatg aaaccagtaa cgttatacga tgtcgcagag
     1741 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct
     1801 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc
     1861 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg
     1921 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt
     1981 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg
     2041 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag
     2101 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct
     2161 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg
     2221 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct
     2281 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag
     2341 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa
     2401 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg
     2461 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggacat ctcggtagtg
     2521 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag
     2581 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag
     2641 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg
     2701 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     2761 caggtttccc gactggaaag cgggcagagg cctgctgcta acgatgaaaa ctatgctctg
     2821 gtcgcctgat aaccatggtc tagagaaaga ggggaaatac tagatgagca caaaaaagaa
     2881 accattaaca caagagcagc ttgaggacgc acgtcgcctt aaagcaattt atgaaaaaaa
     2941 gaaaaatgaa cttggcttat cccaggaatc tgtcgcagac aagatgggga tggggcagtc
     3001 aggcgttggt gctttattta atggcatcaa tgcattaaat gcttataacg ccgcattgct
     3061 tgcaaaaatt ctcaaagtta gcgttgaaga atttagccct tcaatcgcca gagaaatcta
     3121 cgagatgtat gaagcggtta gtatgcagcc gtcacttaga agtgagtatg agtaccctgt
     3181 tttttctcat gttcaggcag ggatgttctc acctgagctt agaaccttta ccaaaggtga
     3241 tgcggagaga tgggtaagca caaccaaaaa agccagtgat tctgcattct ggcttgaggt
     3301 tgaaggtaat tccatgaccg caccaacagg ctccaagcca agctttcctg acggaatgtt
     3361 aattctcgtt gaccctgagc aggctgttga gccaggtgat ttctgcatag ccagacttgg
     3421 gggtgatgag tttaccttca agaaactgat cagggatagc ggtcaggtgt ttttacaacc
     3481 actaaaccca cagtacccaa tgatcccatg caatgagagt tgttccgttg tggggaaagt
     3541 tatcgctagt cagtggcctg aagagacgtt tggcaggcct gctgctaacg atgaaaacta
     3601 tgctctggtc gcctgataat ctagagaaag aggagaaata ctagatgcgt aaaggcgaag
     3661 aactgtttac cggtgtggtt ccgattctgg tggaactgga cggcgatgtt aatggtcata
     3721 aattcagtgt tcgcggcgaa ggtgaaggcg atgcgacgaa cggcaaactg accctgaaat
     3781 ttatctgcac cacgggtaaa ctgccggtcc cgtggccgac gctggtgacc acgctgacct
     3841 atggcgttca atgttttgcg cgttacccgg atcacatgaa acagcacgac tttttcaaat
     3901 cggccatgcc ggaaggctat gtgcaggaac gtacgattag ctttaaagac gatggtacgt
     3961 ataaaacccg cgcggaagtg aaattcgaag gcgataccct ggttaaccgt atcgaactga
     4021 aaggtatcga tttcaaagaa gacggcaata ttctgggtca taaactggaa tataacttca
     4081 attcccacaa cgtgtacatc accgcggata aacagaaaaa cggcattaaa gccaatttca
     4141 aaatccgcca taatgtggaa gatggtagcg ttcagctggc cgaccactat cagcaaaaca
     4201 cgccgattgg tgatggcccg gtcctgctgc cggacaatca ctacctgagt acccagtccg
     4261 tgctgtcaaa agatccgaac gaaaaacgtg accacatggt cctgctggaa tttgtgacgg
     4321 ctgcgggtat cacccacggc atggacgaac tgtataaaag gcctgctgct aacgatgaaa
     4381 actacgcagc tagcgtttga taatagaggc atcaaataaa acgaaaggct cagtcgaaag
     4441 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc
     4501 cctcgagtca ctgcatccta ggagcaaccc agtcagctcc ttccggtggg cgcggggcat
     4561 gactaacatg agaattacaa cttatatcgt atggggctga cttcaggtgc tacatttgaa
     4621 gagataaatt gcactgaaat ctagagcggt tcagtagaaa agatcaaagg atcttcttga
     4681 gatccttttt ttctgcgcgt aatcttttgc cctgtaaacg aaaaaaccac ctggggaggt
     4741 ggtttgatcg aaggttaagc agttccccaa ctgacttaac cttcgaaaaa accacctggg
     4801 gaggtggttt gatcgaaggt taagtcagtt ggggaactgc ttaaccgtgg taactggctt
     4861 tcgcagagca cagcaaccaa atctgtcctt ccagtgtagc cggactttgg cgcacacttc
     4921 aagagcaacc gcgtgtttag ctaaacaaat cctctgcgaa ctcccagtta ccaatggctg
     4981 ctgccagtgg cgttttaccg tgcttttccg ggttggactc aagtgaacag ttaccggata
     5041 aggcgcagca gtcgggctga acggggagtt cttgcttaca gcccagcttg gagcaaacga
     5101 cctacaccga gccgagatac cagtgtgtga gctatgagaa agcgccacac ttcccgtaag
     5161 ggagaaaggc ggaacaggta tccggtaaac ggcagggtcg gaacaggaga gcgcaagagg
     5221 gagcgacccg ccggaaacgg tggggatctt taagtcctgt cgggtttcgc ccgtactgtc
     5281 agattcatgg ttgagcctca cggctcccac agatgcaccg gaaaagcgtc tgtttatgtg
     5341 aactctggca ggagggcgga gcctatggaa aaacgccacc ggcgcggccc tgctgttttg
     5401 cctcacatgt tagtcccctg cttatccacg gaatctgtgg gtaactttgt atgtgtccgc
     5461 agcgcccgcc gcagtctcac gcccggagcg tagcgaccga gtgagctagc tatttgttta
     5521 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5581 caataatatt gaaaaaggaa gagtatgagg gaagcggtga tcgccgaagt atcgactcaa
     5641 ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat
     5701 ttgtacggct ccgcagtgga tgacggcctg aagccacaca gtgatattga tttgctggtt
     5761 acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa
     5821 acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg
     5881 cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg
     5941 cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct
     6001 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa
     6061 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta
     6121 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
//
