LOCUS       pRL055_pDest_attB_acryaa:venus_new      6636 bp    DNA     circular SYN 29-MAY-2013
DEFINITION  Contig[0033], 7418 bases, 6140 checksum.
ACCESSION   -
DBSOURCE    GenBank
KEYWORDS    -.
SOURCE      Danio rerio (unknown)
  ORGANISM  Danio rerio
            Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Cyprinidae; Danio
FEATURES             Location/Qualifiers
     misc_feature    3..459
                     /note="Oriinal location: 3..459"
                     /note="Unknown feature type:frag  1 to 457 of f1 +
                     orientation cass."
                     /label="Misc_Feature_1"
     source          complement(3..459)
                     /organism="synthetic construct"
                     /db_xref="taxon:32630 [Split]"
                     /note="Oriinal location: complement(<3..>459)"
                     /note="mol_type=other DNA"
                     /label="Source_1"
     gene            667..746
                     /note="Oriinal location: 667..4802"
                     /note="white"
                     /label="Gene_1"
     misc_feature    805..1264
                     /feature_type="Regulatory Region"
                     /label="cryaa"
     CDS             1370..2089
                     /cds_type="ORF"
                     /label="venus"
     primer_bind     2352..2373
                     /ApEinfo_fwdcolor="#777777"
                     /ApEinfo_revcolor="#777777"
                     /note="Oriinal location: 1061..1082"
                     /label="M13R"
     misc_feature    2388..2408
                     /ApEinfo_fwdcolor="#4a33cc"
                     /ApEinfo_revcolor="#4a33cc"
                     /note="Oriinal location: 1097..1117"
                     /label="att4_shared"
     misc_recomb     2388..2512
                     /ApEinfo_fwdcolor="#5d40ff"
                     /ApEinfo_revcolor="#5d40ff"
                     /note="Oriinal location: 1097..1221"
                     /label="attR4"
     misc_feature    complement(2552..2857)
                     /ApEinfo_fwdcolor="#ffbf3f"
                     /ApEinfo_revcolor="#ffbf3f"
                     /note="Oriinal location: complement(1261..1566)"
                     /label="ccdB"
     CDS             complement(3199..3858)
                     /ApEinfo_fwdcolor="#ffbf00"
                     /ApEinfo_revcolor="#ffbf00"
                     /note="Oriinal location: complement(1908..2567)"
                     /label="cmR"
     misc_recomb     3967..4091
                     /ApEinfo_fwdcolor="#5370ff"
                     /ApEinfo_revcolor="#5370ff"
                     /note="Oriinal location: 2676..2800"
                     /label="attR3"
     misc_feature    4071..4085
                     /ApEinfo_fwdcolor="#4259cc"
                     /ApEinfo_revcolor="#4259cc"
                     /note="Oriinal location: 2780..2794"
                     /label="att3_shared"
     primer_bind     complement(4100..4115)
                     /ApEinfo_fwdcolor="#777777"
                     /ApEinfo_revcolor="#777777"
                     /note="Oriinal location: complement(2809..2824)"
                     /label="M13F"
     misc_signal     4139..4423
                     /note="Oriinal location: 4921..5205"
                     /note="attB"
     gene            5651..6508
                     /note="Oriinal location: 6433..7290"
                     /note="Amp"
                     /label="Gene_2"
ORIGIN
        1 CTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC
       61 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA
      121 GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC
      181 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC
      241 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG
      301 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA
      361 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC
      421 CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCC CATTCGCCAT TCAGGCTGCG
      481 CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG
      541 GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG
      601 TAAAACGACG GCCAGTGAAT TGTAATACGA CTCACTATAG GGCGAATTGG GTACGTACCG
      661 GGCCCCTAGT ATGTATGTAA GTTAATAAAA CCCATTTTTG CGGAAAGTAG ATAAAAAAAA
      721 CATTTTTTTT TTTTACTGCA CTGGATCTAG CGGATCCGGG AATTGGGAAT TCGTTAACAG
      781 ATCTGCGGCC GCGGCTCGAG TACCATTAAT AGTGTGCATT CAGTGCAGGA TGTTATGTAT
      841 TTATTTTCTG TAGTGCAAAC TTTTTCAAAG AATTTTCCAG TCTAAGCTTT TCAGTCAAGA
      901 AAAAAAAAAA GCGATTGATT ATCTGTGATA TTTAAAAAAA TTAGCTCTTA AATCTGGTGA
      961 ACCAAGTGGC CACAACACAT TAAATACTGA CGTTCTACAG CCATGTAGCT TCATCTGGTC
     1021 TGGTTTGTTT TGGCAGGCAC TTATTTTCCG GTGATAGTTG GCCACAAGAG TATTGTCTGA
     1081 GCCATTCAGT GCTAGACTGT CATTCTCAGG TCAGAGTCCA CCCCGCTGAT TCTGCTGACA
     1141 ACACTGTTCC CACCATGAGA TAATGCCATT CCAGAGAGAT CCATTTGTAA GCCCCTCTTT
     1201 CTGCAGCACA GGTATATAAC CAGGGGTCTG CCTCCACTAA GGCCGGCACA CATCATTTGG
     1261 GGATCTTTGT ACTGTACTAG CCTCTATTAG CCTCCTACTT GCGTTCAGTT CACATCTTTT
     1321 GTTCATCAGG TGCAATAAGT TACAGTAAGG AGTTACCAGG TCTGACAACA TGGTGAGCAA
     1381 GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA
     1441 CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC
     1501 CCTGAAGCTG ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC
     1561 CCTGGGCTAC GGCCTGCAGT GCTTCGCCCG CTACCCCGAC CACATGAAGC AGCACGACTT
     1621 CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA
     1681 CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT
     1741 CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA
     1801 CAACTACAAC AGCCACAACG TCTATATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGC
     1861 CAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCGGCGTG CAGCTCGCCG ACCACTACCA
     1921 GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCTA
     1981 CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT
     2041 CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGTAAA GCGGCCCGCT
     2101 GATCAGCCTC GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC
     2161 CTTCCTTGAC CCTGGAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG
     2221 CATCGCATTG TCTGAGTAGG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA
     2281 AGGGGGAGGA TTGGGAAGAC AATAGCAGGC ATGCTGGGGA GAGATCGCCG CCACCGCGGT
     2341 GGGTACTCGA GCAGGAAACA GCTATGACCA TGATTACGCC AAGCTATCAA CTTTGTATAG
     2401 AAAAGTTGAA CGAGAAACGT AAAATGATAT AAATATCAAT ATATTAAATT AGATTTTGCA
     2461 TAAAAAACAG ACTACATAAT ACTGTAAAAC ACAACATATC CAGTCACTAT GGTCGACCTG
     2521 CAGACTGGCT GTGTATAAGG GAGCCTGACA TTTATATTCC CCAGAACATC AGGTTAATGG
     2581 CGTTTTTGAT GTCATTTTCG CGGTGGCTGA GATCAGCCAC TTCTTCCCCG ATAACGGAGA
     2641 CCGGCACACT GGCCATATCG GTGGTCATCA TGCGCCAGCT TTCATCCCCG ATATGCACCA
     2701 CCGGGTAAAG TTCACGGGGG ACTTTATCTG ACAGCAGACG TGCACTGGCC AGGGGGATCA
     2761 CCATCCGTCG CCCGGGCGTG TCAATAATAT CACTCTGTAC ATCCACAAAC AGACGATAAC
     2821 GGCTCTCTCT TTTATAGGTG TAAACCTTAA ACTGCATTTC ACCAGCCCCT GTTCTCGTCG
     2881 GCAAAGGAGC CGTTCATTTC AATAAACCGG GCGACCTCAG CCATCCCTTC CTGATTTTCC
     2941 GCTTTCCAGC GTTCGGCACG CAGACGACGG GCTTCATTCT GCATGGTTGT GCTTACCGAA
     3001 CCGGAGATAT TGACATCATA TATGCCTTGA GCAACTGATA GCTGTCGCTG TCAACTGTCA
     3061 CTGTAATACG CTGCTTCATA GCATACCTCT TTTTGACATA CTTCGGGTAT ACATATCAGT
     3121 ATATATTCTT ATACCGCAAA AATCAGCGCG CAAATACGCA TACTGTTATC TGGCTTTTAG
     3181 TAAGCCGGAT CCTCTAGATT ACGCCCCGCC CTGCCACTCA TCGCAGTACT GTTGTAATTC
     3241 ATTAAGCATT CTGCCGACAT GGAAGCCATC ACAAACGGCA TGATGAACCT GAATCGCCAG
     3301 CGGCATCAGC ACCTTGTCGC CTTGCGTATA ATATTTGCCC ATGGTGAAAA CGGGGGCGAA
     3361 GAAGTTGTCC ATATTGGCCA CGTTTAAATC AAAACTGGTG AAACTCACCC AGGGATTGGC
     3421 TGAGACGAAA AACATATTCT CAATAAACCC TTTAGGGAAA TAGGCCAGGT TTTCACCGTA
     3481 ACACGCCACA TCTTGCGAAT ATATGTGTAG AAACTGCCGG AAATCGTCGT GGTATTCACT
     3541 CCAGAGCGAT GAAAACGTTT CAGTTTGCTC ATGGAAAACG GTGTAACAAG GGTGAACACT
     3601 ATCCCATATC ACCAGCTCAC CGTCTTTCAT TGCCATACGG AATTCCGGAT GAGCATTCAT
     3661 CAGGCGGGCA AGAATGTGAA TAAAGGCCGG ATAAAACTTG TGCTTATTTT TCTTTACGGT
     3721 CTTTAAAAAG GCCGTAATAT CCAGCTGAAC GGTCTGGTTA TAGGTACATT GAGCAACTGA
     3781 CTGAAATGCC TCAAAATGTT CTTTACGATG CCATTGGGAT ATATCAACGG TGGTATATCC
     3841 AGTGATTTTT TTCTCCATTT TAGCTTCCTT AGCTCCTGAA AATCTCGACG GATCCTAACT
     3901 CAAAATCCAC ACATTATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG GTGCCTAATG
     3961 CGGCCGCCAT AGTGACTGGA TATGTTGTGT TTTACAGTAT TATGTAGTCT GTTTTTTATG
     4021 CAAAATCTAA TTTAATATAT TGATATTTAT ATCATTTTAC GTTTCTCGTT CAACTTTATT
     4081 ATACATAGTT GATAATTCAC TGGCCGTCGT TTTACGGTAC CTCTAGAGAT CCACTAGTGT
     4141 CGACGATGTA GGTCACGGTC TCGAAGCCGC GGTGCGGGTG CCAGGGCGTG CCCTTGGGCT
     4201 CCCCGGGCGC GTACTCCACC TCACCCATCT GGTCCATCAT GATGAACGGG TCGAGGTGGC
     4261 GGTAGTTGAT CCCGGCGAAC GCGCGGCGCA CCGGGAAGCC CTCGCCCTCG AAACCGCTGG
     4321 GCGCGGTGGT CACGGTGAGC ACGGGACGTG CGACGGCGTC GGCGGGTGCG GATACGCGGG
     4381 GCAGCGTCAG CGGGTTCTCG ACGGTCACGG CGGGCATGTC GACACTAGTT CTAGCCAGCT
     4441 TTTGTTCCCT TTAGTGAGGG TTAATTTCGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC
     4501 CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT
     4561 GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC
     4621 CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG
     4681 GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT
     4741 CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA
     4801 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA
     4861 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC
     4921 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG
     4981 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT
     5041 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT
     5101 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC
     5161 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG
     5221 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG
     5281 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG
     5341 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG
     5401 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA
     5461 GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA
     5521 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA
     5581 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT
     5641 CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT
     5701 CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT
     5761 CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG
     5821 CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT
     5881 CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT
     5941 TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG
     6001 CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA
     6061 AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT
     6121 TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT
     6181 GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC
     6241 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA
     6301 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT
     6361 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT
     6421 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA
     6481 GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT
     6541 ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA
     6601 TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCAC
//
