LOCUS       pCK122_pDESTattB_exorh:mCherry      7061 bp    DNA     circular SYN 29-MAY-2013
DEFINITION  Contig[0033], 7418 bases, 6140 checksum.
ACCESSION   -
DBSOURCE    GenBank
KEYWORDS    -.
SOURCE      Danio rerio (unknown)
  ORGANISM  Danio rerio
            Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Cyprinidae; Danio
FEATURES             Location/Qualifiers
     misc_feature    3..459
                     /note="Oriinal location: 3..459"
                     /note="Unknown feature type:frag  1 to 457 of f1 +
                     orientation cass."
                     /label="Misc_Feature_1"
     source          complement(3..459)
                     /organism="synthetic construct"
                     /db_xref="taxon:32630 [Split]"
                     /note="Oriinal location: complement(<3..>459)"
                     /note="mol_type=other DNA"
                     /label="Source_1"
     gene            667..746
                     /note="Oriinal location: 667..4802"
                     /note="white"
                     /label="Gene_1"
     promoter        801..1855
                     /label="exorh"
     CDS             1856..2563
                     /cds_type="ORF"
                     /label="mcherry"
     polyA_signal    2573..2770
                     /label="SV40 late polyA (from pCS2+)"
     primer_bind     2777..2798
                     /ApEinfo_fwdcolor="#777777"
                     /ApEinfo_revcolor="#777777"
                     /note="Oriinal location: 1061..1082"
                     /label="M13R"
     misc_feature    2813..2833
                     /ApEinfo_fwdcolor="#4a33cc"
                     /ApEinfo_revcolor="#4a33cc"
                     /note="Oriinal location: 1097..1117"
                     /label="att4_shared"
     misc_recomb     2813..2937
                     /ApEinfo_fwdcolor="#5d40ff"
                     /ApEinfo_revcolor="#5d40ff"
                     /note="Oriinal location: 1097..1221"
                     /label="attR4"
     misc_feature    complement(2977..3282)
                     /ApEinfo_fwdcolor="#ffbf3f"
                     /ApEinfo_revcolor="#ffbf3f"
                     /note="Oriinal location: complement(1261..1566)"
                     /label="ccdB"
     CDS             complement(3624..4283)
                     /ApEinfo_fwdcolor="#ffbf00"
                     /ApEinfo_revcolor="#ffbf00"
                     /note="Oriinal location: complement(1908..2567)"
                     /label="cmR"
     misc_recomb     4392..4516
                     /ApEinfo_fwdcolor="#5370ff"
                     /ApEinfo_revcolor="#5370ff"
                     /note="Oriinal location: 2676..2800"
                     /label="attR3"
     misc_feature    4496..4510
                     /ApEinfo_fwdcolor="#4259cc"
                     /ApEinfo_revcolor="#4259cc"
                     /note="Oriinal location: 2780..2794"
                     /label="att3_shared"
     primer_bind     complement(4525..4540)
                     /ApEinfo_fwdcolor="#777777"
                     /ApEinfo_revcolor="#777777"
                     /note="Oriinal location: complement(2809..2824)"
                     /label="M13F"
     misc_signal     4564..4848
                     /note="Oriinal location: 4921..5205"
                     /note="attB"
     attB            4564..4848
     gene            6076..6933
                     /note="Oriinal location: 6433..7290"
                     /note="Amp"
                     /label="Gene_2"
ORIGIN
        1 CTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC
       61 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA
      121 GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC
      181 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC
      241 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG
      301 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA
      361 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC
      421 CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCC CATTCGCCAT TCAGGCTGCG
      481 CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG
      541 GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG
      601 TAAAACGACG GCCAGTGAAT TGTAATACGA CTCACTATAG GGCGAATTGG GTACGTACCG
      661 GGCCCCTAGT ATGTATGTAA GTTAATAAAA CCCATTTTTG CGGAAAGTAG ATAAAAAAAA
      721 CATTTTTTTT TTTTACTGCA CTGGATCTAG CGGATCCGGG AATTGGGAAT TCGTTAACAG
      781 ATCTGCGGCC GCGGCTCGAG GCTCAGCTGG CAGTACTACC GCTCACTCCT GCCCGACGTC
      841 AGCATCACCA TGTGCCCATC CTGCTTCCAG GTGCGCTCAT CTGTGTGTGT GTGTGTGTGT
      901 GTCTCCTCTG TTATTTGTCT GTTTGACTCT ATATGCTTTG TTGTGTGTCT GATGGTTGTG
      961 TGTGTGTGTG TGTGTGTGTG TTTCTCAGAT GTTCCACAGT GAGGACTATG AGCTGTTGGT
     1021 TCTTCAGCAC AGCTGCTGCC CGTTCTGCCG CAGACCCATC GATGAGTCCT GCTAACACAC
     1081 ACTCATGTTT ACCGCGGTGT GATCATCAGT CCTGCTAACA CACACTCATG TTTACCGCGG
     1141 TGTGATCCAC CCTGAGCCGC TGTAAAGCCG TCAGTAAATG CCAGCTCAGC ACAGCTCTGA
     1201 TAGGATTACA CAGATCAACA CAAAGCAGAT TGCAGCGATG CTGGACACAC ACACACACAC
     1261 ACACACACAC ACTCACAGCT CAACACACTC CTGCGCATCC ACACATCCCT CATGTTGGTG
     1321 TTTTTAATAA AGATTTAATA TTTCAGTCTG ATAATCTGTG TGTGTGTGTG TGTGTGTGTG
     1381 TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGAGACCGTG AAGTGTCCCT
     1441 TGTGTTTGGT CTTCATCTTA TGAGGCTCAC ACAGGACTTG CTGTGTGTGT GTGTGTGTGT
     1501 GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTTGTCCAGC AGGAGGGTTG TGTTCTGCAG
     1561 GACACCTGTC TGTCTGTGGA TGAGAGATTT CAGCTCTGTG CAGGAGTGTT TGGATGGAGA
     1621 CGCAGCCTGC ACGCGCTGAT GAGCAGCAGT AGTGTGTGTG TGTGTGTCGT GAGTGTGTGT
     1681 GGTGAGAGCG AGCGCTGCTG TGTCTCCATC TCTGGATTAT CCCCCTGTCT GACCCCAATC
     1741 TGACCACTCA CTCCTGTAAT CTGCCTAATC TGGACTCATG GGGTTCACAT AAGAGCGCAG
     1801 CGCCGGCTCT CCGGGCTTCA TCTGCGGAGG TGTGCCGATC GTCCACTTCT CCATCATGGT
     1861 GAGCAAGGGC GAGGAGGACA ACATGGCCAT CATCAAGGAG TTCATGCGCT TCAAGGTGCA
     1921 CATGGAGGGC TCCGTGAACG GCCACGAGTT CGAGATCGAG GGCGAGGGCG AGGGCCGCCC
     1981 CTACGAGGGC ACCCAGACCG CCAAGCTGAA GGTGACCAAG GGCGGCCCCC TGCCCTTCGC
     2041 CTGGGACATC CTGTCCCCTC AGTTCATGTA CGGCTCCAAG GCCTACGTGA AGCACCCCGC
     2101 CGACATCCCC GACTACTTGA AGCTGTCCTT CCCCGAGGGC TTCAAGTGGG AGCGCGTGAT
     2161 GAACTTCGAG GACGGCGGCG TGGTGACCGT GACCCAGGAC TCCTCCCTGC AGGACGGCGA
     2221 GTTCATCTAC AAGGTGAAGC TGCGCGGCAC CAACTTCCCC TCCGACGGCC CCGTAATGCA
     2281 GAAGAAGACC ATGGGCTGGG AGGCCTCCTC CGAGCGGATG TACCCCGAGG ACGGCGCCCT
     2341 GAAGGGCGAG ATCAAGCAGA GGCTGAAGCT GAAGGACGGC GGCCACTACG ACGCCGAGGT
     2401 CAAGACCACC TACAAGGCCA AGAAGCCCGT GCAGCTGCCC GGCGCCTACA ACGTCAACAT
     2461 CAAGCTGGAC ATCACCTCCC ACAACGAGGA CTACACCATC GTGGAACAGT ACGAGCGCGC
     2521 CGAGGGCCGC CACTCCACCG GCGGCATGGA CGAGCTGTAC AAGATCGATG ATGATCCAGA
     2581 CATGATAAGA TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG
     2641 CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA
     2701 ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA
     2761 GGTTTTTTAA CTCGAGCAGG AAACAGCTAT GACCATGATT ACGCCAAGCT ATCAACTTTG
     2821 TATAGAAAAG TTGAACGAGA AACGTAAAAT GATATAAATA TCAATATATT AAATTAGATT
     2881 TTGCATAAAA AACAGACTAC ATAATACTGT AAAACACAAC ATATCCAGTC ACTATGGTCG
     2941 ACCTGCAGAC TGGCTGTGTA TAAGGGAGCC TGACATTTAT ATTCCCCAGA ACATCAGGTT
     3001 AATGGCGTTT TTGATGTCAT TTTCGCGGTG GCTGAGATCA GCCACTTCTT CCCCGATAAC
     3061 GGAGACCGGC ACACTGGCCA TATCGGTGGT CATCATGCGC CAGCTTTCAT CCCCGATATG
     3121 CACCACCGGG TAAAGTTCAC GGGGGACTTT ATCTGACAGC AGACGTGCAC TGGCCAGGGG
     3181 GATCACCATC CGTCGCCCGG GCGTGTCAAT AATATCACTC TGTACATCCA CAAACAGACG
     3241 ATAACGGCTC TCTCTTTTAT AGGTGTAAAC CTTAAACTGC ATTTCACCAG CCCCTGTTCT
     3301 CGTCGGCAAA GGAGCCGTTC ATTTCAATAA ACCGGGCGAC CTCAGCCATC CCTTCCTGAT
     3361 TTTCCGCTTT CCAGCGTTCG GCACGCAGAC GACGGGCTTC ATTCTGCATG GTTGTGCTTA
     3421 CCGAACCGGA GATATTGACA TCATATATGC CTTGAGCAAC TGATAGCTGT CGCTGTCAAC
     3481 TGTCACTGTA ATACGCTGCT TCATAGCATA CCTCTTTTTG ACATACTTCG GGTATACATA
     3541 TCAGTATATA TTCTTATACC GCAAAAATCA GCGCGCAAAT ACGCATACTG TTATCTGGCT
     3601 TTTAGTAAGC CGGATCCTCT AGATTACGCC CCGCCCTGCC ACTCATCGCA GTACTGTTGT
     3661 AATTCATTAA GCATTCTGCC GACATGGAAG CCATCACAAA CGGCATGATG AACCTGAATC
     3721 GCCAGCGGCA TCAGCACCTT GTCGCCTTGC GTATAATATT TGCCCATGGT GAAAACGGGG
     3781 GCGAAGAAGT TGTCCATATT GGCCACGTTT AAATCAAAAC TGGTGAAACT CACCCAGGGA
     3841 TTGGCTGAGA CGAAAAACAT ATTCTCAATA AACCCTTTAG GGAAATAGGC CAGGTTTTCA
     3901 CCGTAACACG CCACATCTTG CGAATATATG TGTAGAAACT GCCGGAAATC GTCGTGGTAT
     3961 TCACTCCAGA GCGATGAAAA CGTTTCAGTT TGCTCATGGA AAACGGTGTA ACAAGGGTGA
     4021 ACACTATCCC ATATCACCAG CTCACCGTCT TTCATTGCCA TACGGAATTC CGGATGAGCA
     4081 TTCATCAGGC GGGCAAGAAT GTGAATAAAG GCCGGATAAA ACTTGTGCTT ATTTTTCTTT
     4141 ACGGTCTTTA AAAAGGCCGT AATATCCAGC TGAACGGTCT GGTTATAGGT ACATTGAGCA
     4201 ACTGACTGAA ATGCCTCAAA ATGTTCTTTA CGATGCCATT GGGATATATC AACGGTGGTA
     4261 TATCCAGTGA TTTTTTTCTC CATTTTAGCT TCCTTAGCTC CTGAAAATCT CGACGGATCC
     4321 TAACTCAAAA TCCACACATT ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGGTGCC
     4381 TAATGCGGCC GCCATAGTGA CTGGATATGT TGTGTTTTAC AGTATTATGT AGTCTGTTTT
     4441 TTATGCAAAA TCTAATTTAA TATATTGATA TTTATATCAT TTTACGTTTC TCGTTCAACT
     4501 TTATTATACA TAGTTGATAA TTCACTGGCC GTCGTTTTAC GGTACCTCTA GAGATCCACT
     4561 AGTGTCGACG ATGTAGGTCA CGGTCTCGAA GCCGCGGTGC GGGTGCCAGG GCGTGCCCTT
     4621 GGGCTCCCCG GGCGCGTACT CCACCTCACC CATCTGGTCC ATCATGATGA ACGGGTCGAG
     4681 GTGGCGGTAG TTGATCCCGG CGAACGCGCG GCGCACCGGG AAGCCCTCGC CCTCGAAACC
     4741 GCTGGGCGCG GTGGTCACGG TGAGCACGGG ACGTGCGACG GCGTCGGCGG GTGCGGATAC
     4801 GCGGGGCAGC GTCAGCGGGT TCTCGACGGT CACGGCGGGC ATGTCGACAC TAGTTCTAGC
     4861 CAGCTTTTGT TCCCTTTAGT GAGGGTTAAT TTCGAGCTTG GCGTAATCAT GGTCATAGCT
     4921 GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT
     4981 AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC
     5041 ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG
     5101 CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT
     5161 GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT
     5221 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC
     5281 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA
     5341 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     5401 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
     5461 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
     5521 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
     5581 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
     5641 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
     5701 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT
     5761 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
     5821 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
     5881 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA
     5941 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC
     6001 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC
     6061 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT
     6121 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT
     6181 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT
     6241 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC
     6301 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA
     6361 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG
     6421 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT
     6481 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC
     6541 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT
     6601 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG
     6661 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC
     6721 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC
     6781 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT
     6841 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG
     6901 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG
     6961 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA
     7021 ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA C
//
