LOCUS       pET_SUMO_Syt1_96       13200 bp DNA     circular SYN 06-DEC-2023
DEFINITION  Chapman_J8Q_2_SUMO_Syt1_96-421_I367C    DIMER     coverage: 743x.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 6, 2023 from SnapGene 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13200
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      415..1003
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     misc_feature    1189..1328
                     /label=bom
                     /note="basis of mobility region from pBR322"
                     /note="color: #a6acb3"
     CDS             complement(1430..1621)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /note="color: #993366"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(2430..3512)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #993366"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(3513..3590)
                     /gene="lacI"
                     /label=lacI promoter
                     /note="color: #ffffff; direction: LEFT"
     promoter        3899..3917
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    3918..3942
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     RBS             3957..3979
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
                     /note="color: #a6acb3"
     CDS             3999..4016
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     CDS             4026..4043
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /note="color: #cc99b2
                     Cleavage site after base 4037"
                     /translation="LVPRGS"
     CDS             4050..4343
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /note="color: #cc99b2
                     Cleavage site after base 4343"
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     CDS             4347..5327
                     /codon_start=1
                     /label=rSyt1
                     /note="color: #993366"
                     /translation="GGKNAINMKDVKDLGKTMKDQALKDDDAETGLTDGEEKEEPKEEE
                     KLGKLQYSLDYDFQNNQLLVGIIQAAELPALDMGGTSDPYVKVFLLPDKKKKFETKVHR
                     KTLNPVFNEQFTFKVPYSELGGKTLVMAVYDFDRFSKHDIIGEFKVPMNTVDFGHVTEE
                     WRDLQSAEKEEQEKLGDIAFSLRYVPTAGKLTVVILEAKNLKKMDVGGLSDPYVKIHLM
                     QNGKRLKKKKTTIKKNTLNPYYNESFSFEVPFEQIQKVQVVVTVLDYDKCGKNDAIGKV
                     FVGYNSTGAELRHWSDMLANPRRPIAQWHTLQVEEEVDAMLAVKK"
     misc_feature    4890..4892
                     /label=C277A
                     /note="color: #ff0000"
     misc_feature    5160..5162
                     /label=I367C
                     /note="color: #ff0000"
     CDS             5386..5403
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     terminator      5470..5517
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      5554..6009
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     CDS             complement(6101..6916)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6101..6916)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      7038..7626
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     misc_feature    7812..7951
                     /label=bom
                     /note="basis of mobility region from pBR322"
                     /note="color: #a6acb3"
     CDS             complement(8053..8244)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /note="color: #993366"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(9053..10135)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #993366"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(10136..10213)
                     /gene="lacI"
                     /label=lacI promoter
                     /note="color: #ffffff; direction: LEFT"
     promoter        10522..10540
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    10541..10565
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     RBS             10580..10602
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
                     /note="color: #a6acb3"
     CDS             10622..10639
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     CDS             10649..10666
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /note="color: #cc99b2
                     Cleavage site after base 10660"
                     /translation="LVPRGS"
     CDS             10673..10966
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /note="color: #cc99b2
                     Cleavage site after base 10966"
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     CDS             10970..11950
                     /codon_start=1
                     /label=rSyt1
                     /note="color: #993366"
                     /translation="GGKNAINMKDVKDLGKTMKDQALKDDDAETGLTDGEEKEEPKEEE
                     KLGKLQYSLDYDFQNNQLLVGIIQAAELPALDMGGTSDPYVKVFLLPDKKKKFETKVHR
                     KTLNPVFNEQFTFKVPYSELGGKTLVMAVYDFDRFSKHDIIGEFKVPMNTVDFGHVTEE
                     WRDLQSAEKEEQEKLGDIAFSLRYVPTAGKLTVVILEAKNLKKMDVGGLSDPYVKIHLM
                     QNGKRLKKKKTTIKKNTLNPYYNESFSFEVPFEQIQKVQVVVTVLDYDKCGKNDAIGKV
                     FVGYNSTGAELRHWSDMLANPRRPIAQWHTLQVEEEVDAMLAVKK"
     misc_feature    11783..11785
                     /label=I367C
                     /note="color: #ff0000"
     CDS             12009..12026
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     terminator      12093..12140
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      12177..12632
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
ORIGIN
        1 gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata
       61 aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct
      121 ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc
      181 gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg
      241 ttggaattta atcgcggcct agagcaagac gtttcccgtt gaatatggct cataacaccc
      301 cttgtattac tgtttatgta agcagacagt tttattgttc atgaccaaaa tcccttaacg
      361 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
      421 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
      481 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
      541 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
      601 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
      661 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
      721 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
      781 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
      841 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
      901 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
      961 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     1021 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     1081 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     1141 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     1201 ttttctcctt acgcatctgt gcggtatttc acaccgcaat ggtgcactct cagtacaatc
     1261 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
     1321 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     1381 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     1441 caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa
     1501 gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg
     1561 ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca
     1621 ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag
     1681 agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg
     1741 agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat
     1801 gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga
     1861 tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa
     1921 cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt
     1981 cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc
     2041 agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggg gccgccatgc
     2101 cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt
     2161 gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc
     2221 agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt
     2281 gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga
     2341 aggagctgac tgggttgaag gctctcaagg gcatcggtcg agatcccggt gcctaatgag
     2401 tgagctaact tacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     2461 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     2521 gccagggtgg tttttctttt caccagtgag acgggcaaca gctgattgcc cttcaccgcc
     2581 tggccctgag agagttgcag caagcggtcc acgctggttt gccccagcag gcgaaaatcc
     2641 tgtttgatgg tggttaacgg cgggatataa catgagctgt cttcggtatc gtcgtatccc
     2701 actaccgaga tatccgcacc aacgcgcagc ccggactcgg taatggcgcg cattgcgccc
     2761 agcgccatct gatcgttggc aaccagcatc gcagtgggaa cgatgccctc attcagcatt
     2821 tgcatggttt gttgaaaacc ggacatggca ctccagtcgc cttcccgttc cgctatcggc
     2881 tgaatttgat tgcgagtgag atatttatgc cagccagcca gacgcagacg cgccgagaca
     2941 gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc
     3001 acgcccagtc gcgtaccgtc ttcatgggag aaaataatac tgttgatggg tgtctggtca
     3061 gagacatcaa gaaataacgc cggaacatta gtgcaggcag cttccacagc aatggcatcc
     3121 tggtcatcca gcggatagtt aatgatcagc ccactgacgc gttgcgcgag aagattgtgc
     3181 accgccgctt tacaggcttc gacgccgctt cgttctacca tcgacaccac cacgctggca
     3241 cccagttgat cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc
     3301 agactggagg tggcaacgcc aatcagcaac gactgtttgc ccgccagttg ttgtgccacg
     3361 cggttgggaa tgtaattcag ctccgccatc gccgcttcca ctttttcccg cgttttcgca
     3421 gaaacgtggc tggcctggtt caccacgcgg gaaacggtct gataagagac accggcatac
     3481 tctgcgacat cgtataacgt tactggtttc acattcacca ccctgaattg actctcttcc
     3541 gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt cgatggtgtc cgggatctcg
     3601 acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt
     3661 gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc
     3721 cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc
     3781 ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc
     3841 ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaatta
     3901 atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg
     3961 tttaacttta agaaggagat atacatatgg gcagcagcca tcatcatcat catcacggca
     4021 gcggcctggt gccgcgcggc agcgctagca tgtcggactc agaagtcaat caagaagcta
     4081 agccagaggt caagccagaa gtcaagcctg agactcacat caatttaaag gtgtccgatg
     4141 gatcttcaga gatcttcttc aagatcaaaa agaccactcc tttaagaagg ctgatggaag
     4201 cgttcgctaa aagacagggt aaggaaatgg actccttaag attcttgtac gacggtatta
     4261 gaattcaagc tgatcagacc cctgaagatt tggacatgga ggataacgat attattgagg
     4321 ctcacagaga acagattggt ggatccggag gaaagaacgc cattaacatg aaagacgtga
     4381 aagacttagg gaagaccatg aaggatcagg cccttaagga tgacgatgct gaaaccggac
     4441 tgactgatgg agaagaaaag gaagagccca aggaagagga gaaactggga aagctccaat
     4501 attcactgga ctatgacttc cagaataacc agctgttggt gggaatcatc caggctgctg
     4561 aactgcccgc cctggacatg gggggtacat ccgatccata cgtcaaagtc ttcctgctgc
     4621 ctgacaaaaa gaagaaattt gagactaaag tccaccggaa aaccctcaat ccagtcttca
     4681 atgaacaatt tactttcaag gtaccctact cggaattagg tggcaaaacc ctggtgatgg
     4741 ctgtgtatga ctttgatcgc ttctccaagc acgacatcat cggagagttc aaagttccta
     4801 tgaacaccgt ggattttggc catgtgaccg aggagtggcg cgatctccag agcgctgaga
     4861 aagaagagca agagaaactg ggtgacatcg ccttctccct ccgctacgtc cctactgccg
     4921 gcaaactgac tgttgtcatt ctggaagcca agaacctgaa gaagatggat gtgggtggct
     4981 tatctgatcc ctacgtgaag attcacctga tgcagaacgg taagaggctg aagaagaaaa
     5041 agacgacgat taagaagaac acactcaacc cctactacaa cgagtccttc agctttgaag
     5101 ttccgttcga gcaaatccag aaagtgcaag tggtggtaac tgttttggac tatgacaagt
     5161 gtggcaagaa cgacgccatc ggaaaagtct tcgttggtta caacagcact ggggcggagc
     5221 tgcgacactg gtcagacatg ctggccaacc cccggcgacc catcgcacag tggcacactc
     5281 tgcaggtaga ggaggaggtt gatgccatgc tggctgtcaa gaagtaaaag cttaggtatt
     5341 tattcggcgc aaagtgcgtc gggtgatgct gccaacttag tcgagcacca ccaccaccac
     5401 cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
     5461 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
     5521 aaaggaggaa ctatatccgg attggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     5581 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     5641 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     5701 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     5761 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     5821 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     5881 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     5941 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     6001 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     6061 ctaaatacat tcaaatatgt atccgctcat gaattaattc ttagaaaaac tcatcgagca
     6121 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc
     6181 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt
     6241 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa
     6301 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
     6361 aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa
     6421 aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata
     6481 cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca
     6541 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg
     6601 ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat
     6661 gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg
     6721 taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct
     6781 tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat
     6841 acccatataa atcagcatcc atgttggaat ttaatcgcgg cctagagcaa gacgtttccc
     6901 gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg
     6961 ttcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     7021 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     7081 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     7141 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     7201 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     7261 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     7321 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     7381 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     7441 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     7501 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     7561 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     7621 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     7681 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     7741 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     7801 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     7861 aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
     7921 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
     7981 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     8041 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta
     8101 aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag
     8161 ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag
     8221 ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg
     8281 ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca
     8341 tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca
     8401 gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca
     8461 gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt
     8521 ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt
     8581 cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg
     8641 ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat
     8701 gcgcacccgt ggggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt
     8761 ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga
     8821 caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc
     8881 tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat
     8941 agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg
     9001 tcgagatccc ggtgcctaat gagtgagcta acttacatta attgcgttgc gctcactgcc
     9061 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     9121 gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca
     9181 acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg
     9241 tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc
     9301 tgtcttcggt atcgtcgtat cccactaccg agatatccgc accaacgcgc agcccggact
     9361 cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg
     9421 gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt
     9481 cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag
     9541 ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt
     9601 gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa
     9661 tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg
     9721 cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga
     9781 cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta
     9841 ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa
     9901 tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt
     9961 tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt
    10021 ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg
    10081 tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca
    10141 ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc
    10201 attcgatggt gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaagcag
    10261 cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag
    10321 atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg
    10381 ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg
    10441 ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc
    10501 gagatctcga tcccgcgaaa ttaatacgac tcactatagg ggaattgtga gcggataaca
    10561 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgggcagcag
    10621 ccatcatcat catcatcacg gcagcggcct ggtgccgcgc ggcagcgcta gcatgtcgga
    10681 ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca
    10741 catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac
    10801 tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt
    10861 aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat
    10921 ggaggataac gatattattg aggctcacag agaacagatt ggtggatccg gaggaaagaa
    10981 cgccattaac atgaaagacg tgaaagactt agggaagacc atgaaggatc aggcccttaa
    11041 ggatgacgat gctgaaaccg gactgactga tggagaagaa aaggaagagc ccaaggaaga
    11101 ggagaaactg ggaaagctcc aatattcact ggactatgac ttccagaata accagctgtt
    11161 ggtgggaatc atccaggctg ctgaactgcc cgccctggac atggggggta catccgatcc
    11221 atacgtcaaa gtcttcctgc tgcctgacaa aaagaagaaa tttgagacta aagtccaccg
    11281 gaaaaccctc aatccagtct tcaatgaaca atttactttc aaggtaccct actcggaatt
    11341 aggtggcaaa accctggtga tggctgtgta tgactttgat cgcttctcca agcacgacat
    11401 catcggagag ttcaaagttc ctatgaacac cgtggatttt ggccatgtga ccgaggagtg
    11461 gcgcgatctc cagagcgctg agaaagaaga gcaagagaaa ctgggtgaca tcgccttctc
    11521 cctccgctac gtccctactg ccggcaaact gactgttgtc attctggaag ccaagaacct
    11581 gaagaagatg gatgtgggtg gcttatctga tccctacgtg aagattcacc tgatgcagaa
    11641 cggtaagagg ctgaagaaga aaaagacgac gattaagaag aacacactca acccctacta
    11701 caacgagtcc ttcagctttg aagttccgtt cgagcaaatc cagaaagtgc aagtggtggt
    11761 aactgttttg gactatgaca agtgtggcaa gaacgacgcc atcggaaaag tcttcgttgg
    11821 ttacaacagc actggggcgg agctgcgaca ctggtcagac atgctggcca acccccggcg
    11881 acccatcgca cagtggcaca ctctgcaggt agaggaggag gttgatgcca tgctggctgt
    11941 caagaagtaa aagcttaggt atttattcgg cgcaaagtgc gtcgggtgat gctgccaact
    12001 tagtcgagca ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag
    12061 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
    12121 gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc
    12181 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
    12241 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
    12301 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
    12361 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
    12421 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
    12481 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
    12541 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
    12601 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
    12661 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgaattaa
    12721 ttcttagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc
    12781 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt
    12841 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca
    12901 acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac
    12961 gactgaatcc ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg
    13021 ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga
    13081 ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat
    13141 cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg
//
