LOCUS       pLenti_HsATP13A2       14907 bp DNA     circular SYN 11-DEC-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14907)
  AUTHORS   Gijsbers, Rik
  TITLE     Direct Submission
  JOURNAL   Exported Dec 11, 2023 from SnapGene 7.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1689..1793
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1794..1862,1863..2654)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2825..3413
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3659..3988
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3839..3974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4188..4645
                     /label=gpt
     intron          5122..5187
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5317..5337
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5762..5896
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             6065..6698
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6745..6870
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6807..6810
                     /label=SD
     misc_feature    6898..6899
                     /label=gag frame shift
     misc_feature    7363..7596
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7781..7825
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7970..7972
                     /label=SA
     misc_feature    8118..8235
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        8294..8597
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8598..8801
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             join(8890..10842,10843..12411)
                     /codon_start=1
                     /label=hATP13A2 P652_E653 del
                     /translation="MSADSSPLVGSTPTGYGTLTIGTSIDPLSSSVSSVRLSGYCGSPW
                     RVIGYHVVVWMMAGIPLLLFRWKPLWGVRLRLRPCNLAHAETLVIEIRDKEDSSWQLFT
                     VQVQTEAIGEGSLEPSPQSQAEDGRSQAAVGAVPEGAWKDTAQLHKSEEAKRVLRYYLF
                     QGQRYIWIETQQAFYQVSLLDHGRSCDDVHRSRHGLSLQDQMVRKAIYGPNVISIPVKS
                     YPQLLVDEALNPYYGFQAFSIALWLADHYYWYALCIFLISSISICLSLYKTRKQSQTLR
                     DMVKLSMRVCVCRPGGEEEWVDSSELVPGDCLVLPQEGGLMPCDAALVAGECMVNESSL
                     TGESIPVLKTALPEGLGPYCAETHRRHTLFCGTLILQARAYVGPHVLAVVTRTGFCTAK
                     GGLVSSILHPRPINFKFYKHSMKFVAALSVLALLGTIYSIFILYRNRVPLNEIVIRALD
                     LVTVVVPPALPAAMTVCTLYAQSRLRRQGIFCIHPLRINLGGKLQLVCFDKTGTLTEDG
                     LDVMGVVPLKGQAFLPLVPEPRRLPVGPLLRALATCHALSRLQDTPVGDPMDLKMVEST
                     GWVLEEEPAADSAFGTQVLAVMRPPLWEPQLQAMEEPPVPVSVLHRFPFSSALQRMSVV
                     VAWPGATQPEAYVKGSLVAGLCNPETVPTDFAQMLQSYTAAGYRVVALASKPLPTVPSL
                     EAAQQLTRDTVEGDLSLLGLLVMRNLLKPQTTPVIQALRRTRIRAVMVTGDNLQTAVTV
                     ARGCGMVAPQEHLIIVHATHPERGQPASLEFLPMESPTAVNGVKDPDQAASYTVEPDPR
                     SRHLALSGPTFGIIVKHFPKLLPKVLVQGTVFARMAPEQKTELVCELQKLQYCVGMCGD
                     GANDCGALKAADVGISLSQAEASVVSPFTSSMASIECVPMVIREGRCSLDTSFSVFKYM
                     ALYSLTQFISVLILYTINTNLGDLQFLAIDLVITTTVAVLMSRTGPALVLGRVRPPGAL
                     LSVPVLSSLLLQMVLVTGVQLGGYFLTLAQPWFVPLNRTVAAPDNLPNYENTVVFSLSS
                     FQYLILAAAVSKGAPFRRPLYTNVPFLVALALLSSVLVGLVLVPGLLQGPLALRNITDT
                     GFKLLLLGLVTLNFVGAFMLESVLDQCLPACLRRLRPKRASKKRFKQLERELAEQPWPP
                     LPAGPLR"
     primer_bind     8893..8917
                     /label=73-005_ATP13A2_intern_S1
     misc_feature    8923..8925
                     /label=T12
     primer_bind     8929..8948
                     /label=qPCR KO F
     primer_bind     complement(9023..9042)
                     /label=qPCR KO R
     primer_bind     9193..9212
                     /label=69-048_HsATP13A2_S2
     primer_bind     9209..9224
                     /label=73-006_ATP13A2_intern_AS1
     primer_bind     complement(9213..9232)
                     /label=69-063_HsATP13A2_AS9
     primer_bind     complement(9479..9502)
                     /label=73-006_ATP13A2_intern_AS1
     primer_bind     9578..9597
                     /label=69-049_HsATP13A2_S3
     primer_bind     complement(9598..9617)
                     /label=69-062_HsATP13A2_AS8
     primer_bind     9964..9985
                     /label=69-050_HsATP13A2_S4
     primer_bind     complement(9998..10017)
                     /label=69-061_HsATP13A2_AS7
     misc_feature    10105..10107
                     /label=I406
     primer_bind     10364..10383
                     /label=69-051_HsATP13A2_S5
     primer_bind     complement(10550..10572)
                     /label=73-006_ATP13A2_intern_AS1
     primer_bind     complement(10817..10836)
                     /label=69-059_HsATP13A2_AS5
     primer_bind     11172..11191
                     /label=69-053_HsATP13A2_S7
     misc_feature    11194..11196
                     /label=V771
     primer_bind     complement(11219..11240)
                     /label=69-058_HsATP13A2_AS4
     misc_feature    11428..11430
                     /label=M849
     primer_bind     11689..11708
                     /label=69-054_HsATP13A2_S8
     misc_feature    11704..11706
                     /label=I941
     primer_bind     complement(11709..11728)
                     /label=69-057_HsATP13A2_AS3
     misc_feature    11869..11871
                     /label=V996
     misc_feature    11926..11928
                     /label=Y1015
     primer_bind     12086..12103
                     /label=69-055_HsATP13A2_S9
     primer_bind     complement(12279..12298)
                     /label=69-056_HsATP13A2_AS2
     misc_feature    12459..13010
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             13010..13612
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     misc_feature    13632..14220
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(14103..14114)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             14390..14570
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    join(14545..14907,1..1063)
                     /label=Hs chr.3 flanking seq
ORIGIN
        1 acacaagagg aaactgagag ggaaagatgt tgaagtaatt ttcccacaat tacagcatcc
       61 gttagttacg actctatgat cttctgacac aaattccatt tactcctcac cctatgactc
      121 agtcgaatat atcaaagtta tggacattat gctaagtaac aaattaccct tttatatagt
      181 aaatactgag tagattgaga gaagaaattg tttgcaaacc tgaatagctt caagaagaag
      241 agaagtgagg ataagaataa cagttgtcat ttaacaagtt ttaacaagta acttggttag
      301 aaagggattc aaatgcataa agcaagggat aaatttttct ggcaacaaga ctatacaata
      361 taaccttaaa tatgacttca aataattgtt ggaacttgat aaaactaatt aaatattatt
      421 gaagattatc aatattataa atgtaattta cttttaaaaa gggaacatag aaatgtgtat
      481 cattagagta gaaaacaatc cttattatca caatttgtca aaacaagttt gttattaaca
      541 caagtagaat actgcattca attaagttga ctgcagattt tgtgttttgt taaaattaga
      601 aagagataac aacaatttga attattgaaa gtaacatgta aatagttcta catacgttct
      661 tttgacatct tgttcaatca ttgatcgaag ttctttatct tggaagaatt tgttccaaag
      721 actctgaaat aaggaaaaca atctattata tagtctcaca cctttgtttt acttttagtg
      781 atttcaattt aataatgtaa atggttaaaa tttattcttc tctgagatca tttcacattg
      841 cagatagaaa acctgagact ggggtaattt ttattaaaat ctaatttaat ctcagaaaca
      901 catctttatt ctaacatcaa tttttccagt ttgatattat catataaagt cagccttcct
      961 catctgcagg ttccacaaca aaaatccaac caactgtgga tcaaaaatat tgggaaaaaa
     1021 ttaaaaatag caatacaaca ataaaaaaat acaaatcaga aaaacagcac agtataacaa
     1081 ctttatttag catttacaat ctattaggta ttataagtaa tctagccaga tcctctacgc
     1141 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
     1201 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
     1261 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc
     1321 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat
     1381 gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg
     1441 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     1501 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     1561 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     1621 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     1681 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1741 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1801 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1861 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     1921 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1981 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     2041 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     2101 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     2161 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     2221 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     2281 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     2341 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     2401 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     2461 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     2521 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     2581 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     2641 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     2701 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     2761 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2821 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2881 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2941 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     3001 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3061 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3121 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3181 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3241 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3301 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3361 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3421 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3481 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3541 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3601 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt
     3661 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3721 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     3781 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     3841 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3901 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     3961 cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat
     4021 gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact
     4081 catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc
     4141 cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat
     4201 acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc
     4261 cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt
     4321 actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca
     4381 cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat
     4441 cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc
     4501 aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta
     4561 tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt
     4621 cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct
     4681 ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga
     4741 cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga
     4801 cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta
     4861 aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg
     4921 acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg
     4981 acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg
     5041 ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct
     5101 acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta
     5161 ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag
     5221 tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat
     5281 gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa
     5341 gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat
     5401 agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag
     5461 aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac
     5521 atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa
     5581 aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat
     5641 agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     5701 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     5761 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     5821 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     5881 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
     5941 cccataccct attaccactg ccaattacct agtggtttca tttactctaa acctgtgatt
     6001 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
     6061 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
     6121 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
     6181 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
     6241 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
     6301 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
     6361 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
     6421 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
     6481 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
     6541 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     6601 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     6661 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     6721 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     6781 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     6841 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     6901 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     6961 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     7021 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     7081 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     7141 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     7201 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     7261 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     7321 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     7381 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     7441 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     7501 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     7561 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     7621 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     7681 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     7741 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     7801 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     7861 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     7921 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     7981 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     8041 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     8101 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     8161 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     8221 aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg gggcgataag
     8281 cttgggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     8341 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     8401 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     8461 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     8521 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     8581 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     8641 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     8701 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     8761 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
     8821 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccga ctctagaaga
     8881 tctgccacca tgagcgcaga cagcagccct ctcgtgggca gcacgcccac cggttatggg
     8941 accctgacga tagggacatc aatagatccc ctcagctcct cagtttcatc cgtgaggctc
     9001 agcggctact gtggcagtcc atggagggtc atcggctatc acgtcgtggt ctggatgatg
     9061 gctgggatcc ctttgctgct cttccgttgg aagcccctgt ggggggtgcg gctgcggctc
     9121 cggccctgca acctggccca cgccgaaaca ctcgttatcg aaataagaga caaagaggat
     9181 agttcctggc agctcttcac tgtccaggtg cagactgagg ccatcggcga gggcagcctg
     9241 gagccgtccc cacagtccca ggcagaggat ggccggagcc aggcggcagt tggggcggta
     9301 ccagagggtg cctggaagga tacggcccag ctccacaaga gcgaggaggc gaagcgggtg
     9361 ctgcggtatt acctcttcca gggccagcgc tatatctgga tcgagaccca gcaagccttc
     9421 taccaggtca gcctcctgga ccatggccgc tcttgtgacg acgtccaccg ctcccgccat
     9481 ggcctcagcc tccaggacca aatggtgagg aaggccattt acggccccaa cgtgatcagc
     9541 ataccggtca agtcctaccc ccagctgctg gtggacgagg cactgaaccc ctactatggg
     9601 ttccaggcct tcagcatcgc gctgtggctg gctgaccact actactggta cgccctgtgc
     9661 atcttcctca tttcctccat ctccatctgc ctgtcgctgt acaagaccag aaagcaaagc
     9721 cagactctaa gggacatggt caagttgtcc atgcgggtgt gcgtgtgccg gccaggggga
     9781 gaggaagagt gggtggactc cagtgagcta gtgcccggag actgcctggt gctgccccag
     9841 gagggtgggc tgatgccctg tgatgccgcc ctggtggccg gcgagtgcat ggtgaatgag
     9901 agctctctga caggagagag cattccagtg ctgaagacgg cactgccgga ggggctgggg
     9961 ccctactgtg cagagacaca ccggcggcac acactcttct gcgggaccct catcttgcag
    10021 gcccgggcct atgtgggacc gcacgtcctg gcagtggtga cccgcacagg gttctgcacg
    10081 gcaaaagggg gcctggtgag ctccatcttg cacccccggc ccatcaactt caagttctat
    10141 aaacacagca tgaagtttgt ggctgccctc tctgtcctgg ctctcctcgg caccatctac
    10201 agcatcttca tcctctaccg aaaccgggtg cctctgaatg agattgtaat ccgggctctc
    10261 gacctggtga ccgtggtggt gccacctgcc ctgcctgctg ccatgactgt gtgcacgctc
    10321 tacgcccaga gccgactgcg gagacagggc attttctgca tccacccact gcgcatcaac
    10381 ctggggggca agctgcagct ggtgtgtttc gacaagacgg gcaccctcac tgaggacggc
    10441 ttagacgtga tgggggtggt gcccctgaag gggcaggcat tcctgcccct ggtcccagag
    10501 cctcgccgcc tgcctgtggg gcccctgctc cgagcactgg ccacctgcca tgccctcagc
    10561 cggctccagg acacccccgt gggcgacccc atggacttga agatggtgga gtctactggc
    10621 tgggtcctgg aggaagagcc ggctgcagac tcagcatttg ggacccaggt cttggcagtg
    10681 atgagacccc cactttggga gccccagctg caggcaatgg aggagccccc ggtgccagtc
    10741 agcgtcctcc accgcttccc cttctcttcg gctctgcagc gcatgagtgt ggtggtggcg
    10801 tggccagggg ccactcagcc cgaggcctac gtcaaaggct ccctggtggc agggctctgc
    10861 aaccccgaga cagtgcccac cgacttcgcc cagatgctgc agagctatac agctgctggc
    10921 taccgtgtcg tggccctggc cagcaagcca ctgcccactg tgcccagcct ggaggcagcc
    10981 cagcaactga cgagggacac tgtggaagga gacctgagcc tcctggggct gctggtcatg
    11041 aggaacctac tgaagccgca gacaacgcca gttatccagg ctctgcgaag gacccgcatc
    11101 cgcgccgtca tggtgacagg ggacaacctg cagacagcgg tgactgtggc ccggggctgt
    11161 ggcatggtgg ccccccagga gcatctgatc atcgtccacg ccacccaccc tgagcggggt
    11221 cagcctgcct ctctcgagtt cctgccgatg gagtccccca cagccgtgaa tggcgttaag
    11281 gatcctgacc aggctgcaag ctacaccgtg gagccagacc cccgatccag gcacctggcc
    11341 ctcagcgggc ccacctttgg tatcattgtg aagcacttcc ccaagctgct gcccaaggtc
    11401 ctggtccagg gcactgtctt tgcccgcatg gcccctgagc agaagacaga gctggtgtgc
    11461 gagctacaga agcttcagta ctgcgtgggc atgtgcggag acggcgccaa tgactgtggg
    11521 gccctgaagg cggctgatgt cggcatctcg ctgtcccagg cagaagcctc agtggtctca
    11581 cccttcacct cgagcatggc cagtattgag tgcgtgccca tggtcatcag ggaggggcgc
    11641 tgttcccttg acacttcatt cagcgtcttc aagtacatgg ctctgtacag cctgacccag
    11701 ttcatctccg tcctgatcct ctacacgatc aacaccaacc tgggtgacct gcagttcctg
    11761 gccatcgacc tggtcatcac caccacagtg gcagtgctca tgagccgcac ggggccagcg
    11821 ctggtcctgg gacgggtgcg gccaccgggg gcgctgctca gcgtgcccgt gctcagcagc
    11881 ctgctgctgc agatggtcct ggtgaccggc gtgcagctag ggggctactt cctgaccctg
    11941 gcccagccat ggttcgtgcc tctgaacagg acagtggccg caccagacaa cctgcccaac
    12001 tacgagaaca ccgtggtctt ctctctgtcc agcttccagt acctcatcct ggctgcagcc
    12061 gtgtccaagg gggcgccctt ccgccggccg ctctacacca atgtgccctt cctggtggcc
    12121 ctggcgctcc tgagctccgt cctggtgggt cttgtcctgg tccccggcct cctgcagggg
    12181 ccgctggcgc tgaggaacat cactgacacc ggcttcaagc tgctgctgct gggtctggtc
    12241 accctcaact tcgtgggggc cttcatgctg gagagcgtgc tagaccagtg cctccccgcc
    12301 tgcctgcgcc gcctccggcc caagcgggcc tccaagaagc gcttcaagca gctggaacga
    12361 gagctggccg agcagccctg gccgccgctg cccgccggcc ccctgaggta gactagtacg
    12421 cgtgctctag cccaattccg ccccctcccc cccccctaac gttactggcc gaagccgctt
    12481 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
    12541 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
    12601 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
    12661 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
    12721 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
    12781 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
    12841 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
    12901 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa acgtctaggc
    12961 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata tgggcaccga
    13021 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct
    13081 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat
    13141 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
    13201 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
    13261 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
    13321 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
    13381 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
    13441 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
    13501 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
    13561 gtgcccgaag gaccgcgcga cctggtgcat gacccgcaag cccggtgcct gaggcgcgtt
    13621 gaattcccga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
    13681 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
    13741 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
    13801 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
    13861 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
    13921 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
    13981 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
    14041 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
    14101 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
    14161 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
    14221 atcgggaatt ctgtcgactg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
    14281 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
    14341 aaaatcgtcg agagatgctg catataagca gctgcttttt gcttgtactg ggtctctctg
    14401 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
    14461 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
    14521 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
    14581 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
    14641 gccttgacat tataatagat ttagcaggaa ttgaactagg agtggagcac acaggcaaag
    14701 ctgcagaagt acttggaaga agccaccaga gatactcacg attctgcaca tacctggcta
    14761 atcccagatc ctaaggatta cattaagttt actaacattt atataatgat ttatagttta
    14821 aagtataaac ttatctaatt tactattctg acagatatta attaatcctc aaatatcata
    14881 agagatgatt actattatcc ccattta
//
