LOCUS       DmREDusk-MCS_pSM        7989 bp DNA     circular SYN 08-DEC-2023
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 8, 2023 from SnapGene Viewer 7.0.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7989
                     /note="/ugene_group=Source"
                     /note="/ugene_name=Synthetic\ DNA\ construct"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     terminator      26..73
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
                     /note="/ugene_group=Terminator"
                     /note="/ugene_name=T7\ terminator"
                     /note="color: #ffffff"
     CDS             complement(140..157)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="/ugene_group=CDS"
                     /note="/ugene_name=Hexahistidin-tag"
                     /note="color: #993366"
                     /translation="HHHHHH"
     CDS             complement(158..203)
                     /label=multiple-cloning site
                     /note="/ugene_group=CDS"
                     /note="/ugene_name=MCS"
                     /note="color: #993366; direction: LEFT"
     CDS             complement(205..237)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /note="color: #cc99b2"
                     /translation="MASMTGGQQMG"
     CDS             complement(241..258)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /note="color: #cc99b2
                     Cleavage site after base 246"
                     /translation="LVPRGS"
     CDS             complement(268..285)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     CDS             complement(295..297)
                     /codon_start=1
                     /label=start codon
                     /note="color: #ffff00"
                     /translation="M"
     RBS             304..326
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
                     /note="color: #a6acb3"
     promoter        complement(339..585)
                     /label=FixK2 promoter
                     /note="/ugene_group=Promoter"
                     /note="/ugene_name=FixK2\ promoter"
                     /note="color: #ffffff; direction: LEFT"
     promoter        880..957
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
                     /note="/ugene_group=Promoter"
                     /note="/ugene_name=lacIq\ promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             961..1566
                     /label=Dr heme oxygenase
                     /note="/ugene_group=CDS"
                     /note="/ugene_name=DrHo"
                     /note="color: #993366; direction: RIGHT"
     CDS             1563..3131
                     /label=DmBphP
                     /note="/ugene_group=CDS"
                     /note="/ugene_name=DmBphP"
                     /note="color: #993366; direction: RIGHT"
     CDS             3132..3869
                     /label=FixL
                     /note="Histidine kinase"
                     /note="/ugene_group=CDS"
                     /note="/ugene_name=BjFixL"
                     /note="color: #993366; direction: RIGHT"
     CDS             3866..4483
                     /label=FixJ
                     /note="/ugene_group=CDS"
                     /note="/ugene_name=FixJ"
                     /note="color: #993366; direction: RIGHT"
     CDS             5284..5475
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /note="color: #993366"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    5577..5719
                     /label=bom
                     /note="basis of mobility region from pBR322"
                     /note="/ugene_group=Misc\ Feature"
                     /note="/ugene_name=bom"
                     /note="color: #a6acb3"
     rep_origin      complement(5905..6493)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="/ugene_group=Replication\ Origin"
                     /note="/ugene_name=ori"
                     /note="color: #ffff00"
     CDS             6615..7430
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      complement(7523..7978)
                     /direction=LEFT
                     /label=f1 bacteriophage origin of replication
                     /note="f1 bacteriophage origin of replication"
                     /note="/ugene_group=Replication\ Origin"
                     /note="/ugene_name=f1\ ori"
                     /note="color: #ffff00"
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
      181 cgacggagct cgaattcgga tccgacccat ttgctgtcca ccagtcatgc tagccatatg
      241 gctgccgcgc ggcaccaggc cgctgctgtg atgatgatga tgatggctgc tgcccatggt
      301 atatctcctt cttaaagtta aacaaaatta tttctagact ccgttgtgat gacgcattgg
      361 tacgcggtat cgggaggttc gaaaatttcg agcgatatct taaggggggt gccttacgta
      421 gaaccccgta ggtcatgccc gaggccggtc ctggatggcg cggcggatac gcttgagcag
      481 gttttcgtcg agaagcggct tcaaaaccac gtcttttacg ccggcctcgg cggcccgggt
      541 cgagatgttt tcgtccggat agccggtgat caggatcacg ggcgtagatc tcgatcctct
      601 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata
      661 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt
      721 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc
      781 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc
      841 taatgcagga gtcgcataag ggagagcgtc gagatcccgg acaccatcga atggtgcaaa
      901 acctttcgcg gtatggcatg atagcgcccg gaagagagtc aattgagggt ggtgaatgtg
      961 ctccgactca tgatcatgaa gcttttacaa agggagaccc tggacctgca ccgcgaggtc
     1021 gaggccctga tgcccgtgat gagtgagacg ctgacccggc ggggctatct ggaggtgctg
     1081 cggcgcctgc aagcggtggt cgcgccgctg gaagcacaac tcgacgccct gccgctgccg
     1141 gacgagctgg cgtggcccga gcgccgcaag gcccatctgc tcgctctgga ccttgccgta
     1201 atgggggaag ggccgtcaaa tcagcaggcg ccactgtctg tgcccgagct gaccactctg
     1261 gcgcaggcct tcggagcgct ttacgtgctt gaaggcgcga cgctaggcgg acagctcatc
     1321 agccgccatc tcaggcgcac gctggggcta agccctgagc agggcagcgc ctatttctcc
     1381 ggctatggcc cccagaccgg cccgcgctgg cgctcgttcg gggaagtgct ggaagccagc
     1441 gtgccggcag aagacgcggc agaggtcgtg gcgggagcgc ggcagacctt cggggccttc
     1501 cgccgcgcct tgcaggggct gtcggaagct gaagcggtcc aagtgcctga ggtcgcccac
     1561 gcatgacaga acgtccggca gcgcgtccgg gtgcagcacg tatgaccggt ggtgatgatt
     1621 ttctgacacc ggcacattta ggtggtccgc ctgttgatac cgataattgt gcacgtgaac
     1681 cgattcatat tccgggtgcc gttcagccgc atggtgcact gctggttctg cgtgcagatg
     1741 atgaacgtgc agttcaggtt agcgcaaata gcgaagcatt tgttggtgtt gcacctgatg
     1801 cgctgctggg tcagcatctg gccgatctgg ttggtgatca gggtgcagcc gcactgcgtg
     1861 ccgcatttgc acaggcaggt ccgagcgcaa tgaccctgac ctttcgtaat ggtcgtacct
     1921 atgacgttac cgcacgtcgt gatggcgaac tgctgctgat ggaactggaa ccgcctgatg
     1981 ttcgtgcagg cactccggca ctgtatcatg caattcgtga tgcactgggt gcactggaac
     2041 atgcaccgga tctgcatgcc ctgctggatg ttgcagcaca gcgtgttcgt gccctgaccg
     2101 gtctggatcg tgtgatgatc tatcgttttg cagcagatga tagcggtgaa gttgttgccg
     2161 aagcacgtgc accgcattta catgcatttc tgggtcatcg ttttccggaa tcagatattc
     2221 cgcgtcaggc acgtgcgctg tatgtgcagc atttactgcg ttttaccgca gatgccggtg
     2281 gtggtcaggt tccgctggtt cctgcactga atccggttac caatgcaccg ctgcagatgg
     2341 gtgccctggt tttacgtgca accagtccgg ttcatctgca gtatctgcgt aatatgggtg
     2401 ttattgcaag catgagcgtt agcattgttc aggatggtcg tctgtggggt ctgattgcat
     2461 gtcatcatgg tgccgcacat gttgttccgc aggcaacccg tgatgcatgt gaatttctgg
     2521 gacgtgttct gagcctgcag attaccgcaa aacgtgatgc agcagttaat gcccgtcgtg
     2581 cagccctggg tgcccagcat gcacgtctgg ttaccgcagt taccggtacg ctgacaccgc
     2641 tggatgcact gacccgtgcc gatctgaatc tgcctggttt tctggataca gccggtgccg
     2701 cagttcgtat ggatggtggt acacgtaccc tgggtgttac cccgagtgat gaggatctgg
     2761 aacatctggt tgcatggctg cgtgcacagg gtacaccgag cgtttgtacc gatgcgctgg
     2821 cacgtaccta tccgccaggt gcactgttta tggaacgtgc aagcggtgtt ctgggtgtga
     2881 gcattagcgg taattgggat gaatatctgc tgtggtttcg tccggaaatt ccggcaacca
     2941 ttacctgggg tggtgatccg cataaagccg ttcaggtttc agatgatggt acagcacgtc
     3001 tgactccgcg tgccagcttt agcagctatg ttgaagcagt tcgtggtgtt gcccagccgt
     3061 ggcaggcagg cgatctggat gcagcactgg atctgcgtgg tgcattactg gaagttcagg
     3121 gtgcccgtct gctccaggaa ctgcaatccg agctcgtcca cgtctccagg ctgagcgcca
     3181 tgggcgaaat ggcgtccgcg ctcgcgcacg agctcaacca gccgctggcg gcgatcagca
     3241 actacatgaa gggctcgcgg cggctgcttg ccggcagcag tgatccgaac acaccgaagg
     3301 tcgaaagcgc cctggaccgc gccgccgagc aggcgctgcg cgccggccag atcatccggc
     3361 gcctgcgcga cttcgttgcc cgcggcgaat cggagaagcg ggtcgagagt ctctccaagc
     3421 tgatcgagga ggccggcgcg ctcgggcttg ccggcgcgcg cgagcagaac gtgcagctcc
     3481 gcttcagtct cgatccgggc gccgatctcg ttctcgccga ccgggtgcag atccagcagg
     3541 tcctggtcaa cctgttccgc aacgcgctgg aagcgatggc tcagtcgcag cgacgcgagc
     3601 tcgtcgtcac caacaccccc gccgccgacg acatgatcga ggtcgaagtg tccgacaccg
     3661 gcagcggttt ccaggacgac gtcattccga acctgtttca gactttcttc accaccaagg
     3721 acaccggcat gggcgtggga ctgtccatca gccgctcgat catcgaagct cacggcgggc
     3781 gcatgtgggc cgagagcaac gcatcgggcg gggcgacctt ccgcttcacc ctcccggcag
     3841 ccgacgagat gataggaggt ctagcatgac gaccaaggga catatctacg tcatcgacga
     3901 cgacgcggcg atgcgggatt cgctgaattt cctgctggat tctgccggct tcggcgtcac
     3961 gctgtttgac gacgcgcaag cctttctcga cgccctgccg ggtctctcct tcggctgcgt
     4021 cgtctccgac gtgcgcatgc cgggccttga cggcatcgag ctgttgaagc ggatgaaggc
     4081 gcagcaaagc ccctttccga tcctcatcat gaccggtcac ggcgacgtgc cgctcgcggt
     4141 cgaggcgatg aagttagggg cggtggactt tctggaaaag cctttcgagg acgaccgcct
     4201 caccgccatg atcgaatcgg cgatccgcca ggccgagccg gccgccaaga gcgaggccgt
     4261 cgcgcaggat atcgccgccc gcgtcgcctc gttgagcccc agggagcgcc aggtcatgga
     4321 agggctgatc gccggccttt ccaacaagct gatcgcccgc gagtacgaca tcagcccgcg
     4381 caccatcgag gtgtatcggg ccaacgtcat gaccaagatg caggccaaca gcctttcgga
     4441 gctggttcgc ctcgcgatgc gcgccggcat gctcaacgat tgacaattga tgtaagttag
     4501 ctcactcatt aggcaccggg atctcgaccg atgcccttga gagccttcaa cccagtcagc
     4561 tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc
     4621 atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt
     4681 cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc
     4741 gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc
     4801 gccggcatgg cggccccacg ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta
     4861 ggctggcggg gttgccttac tggttagcag aatgaatcac cgatacgcga gcgaacgtga
     4921 agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt cttcggtttc
     4981 cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct gcaccattat gttccggatc
     5041 tgcatcgcag gatgctgctg gctaccctgt ggaacaccta catctgtatt aacgaagcgc
     5101 tggcattgac cctgagtgat ttttctctgg tcccgccgca tccataccgc cagttgttta
     5161 ccctcacaac gttccagtaa ccgggcatgt tcatcatcag taacccgtat cgtgagcatc
     5221 ctctctcgtt tcatcggtat cattaccccc atgaacagaa atccccctta cacggaggca
     5281 tcagtgacca aacaggaaaa aaccgccctt aacatggccc gctttatcag aagccagaca
     5341 ttaacgcttc tggagaaact caacgagctg gacgcggatg aacaggcaga catctgtgaa
     5401 tcgcttcacg accacgctga tgagctttac cgcagctgcc tcgcgcgttt cggtgatgac
     5461 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     5521 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
     5581 gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag
     5641 agcagattgt actgagagtg caccatatat gcggtgtgaa ataccgcaca gatgcgtaag
     5701 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     5761 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     5821 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     5881 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     5941 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     6001 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     6061 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     6121 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     6181 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     6241 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     6301 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     6361 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     6421 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     6481 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     6541 aaactcacgt taagggattt tggtcatgaa caataaaact gtctgcttac ataaacagta
     6601 atacaagggg tgttatgagc catattcaac gggaaacgtc ttgctctagg ccgcgattaa
     6661 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
     6721 caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg tttctgaaac
     6781 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga
     6841 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt
     6901 tactcaccac tgcgatcccc gggaaaacag cattccaggt attagaagaa tatcctgatt
     6961 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg
     7021 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa
     7081 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg
     7141 aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc
     7201 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg
     7261 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc
     7321 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc
     7381 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa gaattaattc
     7441 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     7501 tttccccgaa aagtgccacc tgaaattgta aacgttaata ttttgttaaa attcgcgtta
     7561 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     7621 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     7681 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     7741 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     7801 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     7861 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     7921 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
     7981 cattcgcca
//
