LOCUS       DrDERusk-MCS_pSM        7920 bp DNA     circular SYN 08-DEC-2023
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 8, 2023 from SnapGene Viewer 7.0.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7920
                     /label=/ugene_group=Source
                     /note="/ugene_group=Source"
                     /note="/ugene_name=Synthetic\ DNA\ construct"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     terminator      26..73
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
                     /note="/ugene_group=Terminator"
                     /note="/ugene_name=T7\ terminator"
                     /note="color: #ffffff"
     CDS             complement(140..157)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     CDS             complement(158..203)
                     /label=multiple-cloning site
                     /note="/ugene_group=CDS"
                     /note="/ugene_name=MCS"
                     /note="color: #993366; direction: LEFT"
     CDS             complement(205..237)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /note="color: #cc99b2"
                     /translation="MASMTGGQQMG"
     CDS             complement(241..258)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /note="color: #cc99b2
                     Cleavage site after base 246"
                     /translation="LVPRGS"
     CDS             complement(268..285)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     CDS             complement(295..297)
                     /codon_start=1
                     /label=start codon
                     /note="color: #ffff00"
                     /translation="M"
     RBS             304..326
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
                     /note="color: #a6acb3"
     promoter        complement(339..585)
                     /label=FixK2 promoter
                     /note="/ugene_group=Promoter"
                     /note="/ugene_name=FixK2\ promoter"
                     /note="color: #ffffff; direction: LEFT"
     promoter        880..957
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
                     /note="/ugene_group=Promoter"
                     /note="/ugene_name=lacIq\ promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             961..1566
                     /label=Dr heme oxygenase
                     /note="/ugene_group=CDS"
                     /note="/ugene_name=DrHo"
                     /note="color: #993366; direction: RIGHT"
     CDS             1563..3077
                     /label=DrBphP
                     /note="/ugene_group=CDS"
                     /note="/ugene_name=DrBphP"
                     /note="color: #993366; direction: RIGHT"
     CDS             3078..3800
                     /label=FixL
                     /note="Histidine kinase"
                     /note="/ugene_group=CDS"
                     /note="/ugene_name=BjFixL"
                     /note="color: #993366; direction: RIGHT"
     CDS             3797..4414
                     /label=FixJ
                     /note="/ugene_group=CDS"
                     /note="/ugene_name=FixJ"
                     /note="color: #993366; direction: RIGHT"
     CDS             5215..5406
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /note="color: #993366"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    5508..5650
                     /label=bom
                     /note="basis of mobility region from pBR322"
                     /note="/ugene_group=Misc\ Feature"
                     /note="/ugene_name=bom"
                     /note="color: #a6acb3"
     rep_origin      complement(5836..6424)
                     /direction=LEFT
                     /label=/ugene_group=Replication  Origin
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="/ugene_group=Replication\ Origin"
                     /note="/ugene_name=ori"
                     /note="color: #ffff00"
     CDS             6546..7361
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      complement(7454..7909)
                     /direction=LEFT
                     /label=f1 bacteriophage origin of replication
                     /note="f1 bacteriophage origin of replication"
                     /note="/ugene_group=Replication\ Origin"
                     /note="/ugene_name=f1\ ori"
                     /note="color: #ffff00"
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
      181 cgacggagct cgaattcgga tccgacccat ttgctgtcca ccagtcatgc tagccatatg
      241 gctgccgcgc ggcaccaggc cgctgctgtg atgatgatga tgatggctgc tgcccatggt
      301 atatctcctt cttaaagtta aacaaaatta tttctagact ccgttgtgat gacgcattgg
      361 tacgcggtat cgggaggttc gaaaatttcg agcgatatct taaggggggt gccttacgta
      421 gaaccccgta ggtcatgccc gaggccggtc ctggatggcg cggcggatac gcttgagcag
      481 gttttcgtcg agaagcggct tcaaaaccac gtcttttacg ccggcctcgg cggcccgggt
      541 cgagatgttt tcgtccggat agccggtgat caggatcacg ggcgtagatc tcgatcctct
      601 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata
      661 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt
      721 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc
      781 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc
      841 taatgcagga gtcgcataag ggagagcgtc gagatcccgg acaccatcga atggtgcaaa
      901 acctttcgcg gtatggcatg atagcgcccg gaagagagtc aattgagggt ggtgaatgtg
      961 ctccgactca tgatcatgaa gcttttacaa agggagaccc tggacctgca ccgcgaggtc
     1021 gaggccctga tgcccgtgat gagtgagacg ctgacccggc ggggctatct ggaggtgctg
     1081 cggcgcctgc aagcggtggt cgcgccgctg gaagcacaac tcgacgccct gccgctgccg
     1141 gacgagctgg cgtggcccga gcgccgcaag gcccatctgc tcgctctgga ccttgccgta
     1201 atgggggaag ggccgtcaaa tcagcaggcg ccactgtctg tgcccgagct gaccactctg
     1261 gcgcaggcct tcggagcgct ttacgtgctt gaaggcgcga cgctaggcgg acagctcatc
     1321 agccgccatc tcaggcgcac gctggggcta agccctgagc agggcagcgc ctatttctcc
     1381 ggctatggcc cccagaccgg cccgcgctgg cgctcgttcg gggaagtgct ggaagccagc
     1441 gtgccggcag aagacgcggc agaggtcgtg gcgggagcgc ggcagacctt cggggccttc
     1501 cgccgcgcct tgcaggggct gtcggaagct gaagcggtcc aagtgcctga ggtcgcccac
     1561 gcatgagccg ggacccgttg cccttttttc caccgcttta ccttggtggc ccggaaatta
     1621 ccaccgagaa ctgcgagcgc gagccgattc atattcccgg cagcatccag ccgcacggcg
     1681 ccctgctcac tgccgacggg cacagcggcg aggtgctcca gatgagcctc aacgcggcca
     1741 cttttctggg acaggaaccc acagtgctgc gcggacagac cctcgccgca ctgctgcccg
     1801 agcagtggcc cgcgctgcaa gcggccctgc cccccggctg ccccgacgcc ctgcaatacc
     1861 gcgcaacgct ggactggcct gccgccgggc acctttcgct gacggtgcac cgggtcggcg
     1921 agttgctgat tctggaattc gagccgacgg aggcctggga cagcaccggg ccgcacgcgc
     1981 tgcgcaacgc gatgttcgcg ctcgaaagtg cccccaacct gcgggcgctg gccgaggtgg
     2041 cgacccagac ggtccgcgag ctgacgggct ttgaccgggt gatgctctac aaatttgccc
     2101 ccgacgccac cggcgaagtg attgccgagg cccgccgtga ggggctgcac gcctttctgg
     2161 gccaccgttt tcccgcgtcg gacattccgg cgcaggcccg cgcgctctac acccggcacc
     2221 tgctgcgcct gaccgccgac acccgcgccg ccgccgtgcc gctcgatccc gtcctcaacc
     2281 cgcagacgaa tgcgcccacc ccgctgggcg gcgccgtgct gcgcgccacc tcgcccatgc
     2341 acatgcagta cctgcggaac atgggcgtcg ggtcgagcct gtcggtgtcg gtggtggtcg
     2401 gcggccagct ctggggcctg atcgcctgcc accaccagac gccctacgtg ttgccgcccg
     2461 acctgcgaac cacgctcgaa tacctgggcc gcttgctgag cctgcaagtt caggtcaagg
     2521 aagcggcgga cgtggcggcc tttcgccaga gcctgcggga gcaccacgcg cgggtggccc
     2581 tcgcggcggc gcactcgctc tcgccgcacg acaccctcag tgacccggcg cttgacctgc
     2641 tgggcctgat gcgggccggg ggcctgattc tgcgtttcga gggccgctgg cagacgttgg
     2701 gtgaagtgcc gcctgccccg gcggtggacg cgctgctggc gtggctcgaa acccagccgg
     2761 gcgccctggt ccagaccgac gcgctaggcc aactgtggcc cgccggcgcc gatctcgccc
     2821 ccagcgcagc gggcctgctc gccatcagcg tgggcgaggg ctggtcggag tgcctcgtct
     2881 ggctgcggcc cgaactgcgg ctggaggtcg cctggggcgg ggccactcct gaccaggcga
     2941 aagacgacct cgggccgcgc cactcattcg acacctacct cgaagaaaaa cgcggctacg
     3001 ccgagccctg gcatcccggc gaaatcgagg aggcgcagga tctacgtgac acattgaccg
     3061 gggcgctggg cgagcgctcc gagctcgtcc acgtctccag gctgagcgcc atgggcgaaa
     3121 tggcgtccgc gctcgcgcac gagctcaacc agccgctggc ggcgatcagc aactacatga
     3181 agggctcgcg gcggctgctt gccggcagca gtgatccgaa cacaccgaag gtcgaaagcg
     3241 ccctggaccg cgccgccgag caggcgctgc gcgccggcca gatcatccgg cgcctgcgcg
     3301 acttcgttgc ccgcggcgaa tcggagaagc gggtcgagag tctctccaag ctgatcgagg
     3361 aggccggcgc gctcgggctt gccggcgcgc gcgagcagaa cgtgcagctc cgcttcagtc
     3421 tcgatccggg cgccgatctc gttctcgccg accgggtgca gatccagcag gtcctggtca
     3481 acctgttccg caacgcgctg gaagcgatgg ctcagtcgca gcgacgcgag ctcgtcgtca
     3541 ccaacacccc cgccgccgac gacatgatcg aggtcgaagt gtccgacacc ggcagcggtt
     3601 tccaggacga cgtcattccg aacctgtttc agactttctt caccaccaag gacaccggca
     3661 tgggcgtggg actgtccatc agccgctcga tcatcgaagc tcacggcggg cgcatgtggg
     3721 ccgagagcaa cgcatcgggc ggggcgacct tccgcttcac cctcccggca gccgacgaga
     3781 tgataggagg tctagcatga cgaccaaggg acatatctac gtcatcgacg acgacgcggc
     3841 gatgcgggat tcgctgaatt tcctgctgga ttctgccggc ttcggcgtca cgctgtttga
     3901 cgacgcgcaa gcctttctcg acgccctgcc gggtctctcc ttcggctgcg tcgtctccga
     3961 cgtgcgcatg ccgggccttg acggcatcga gctgttgaag cggatgaagg cgcagcaaag
     4021 cccctttccg atcctcatca tgaccggtca cggcgacgtg ccgctcgcgg tcgaggcgat
     4081 gaagttaggg gcggtggact ttctggaaaa gcctttcgag gacgaccgcc tcaccgccat
     4141 gatcgaatcg gcgatccgcc aggccgagcc ggccgccaag agcgaggccg tcgcgcagga
     4201 tatcgccgcc cgcgtcgcct cgttgagccc cagggagcgc caggtcatgg aagggctgat
     4261 cgccggcctt tccaacaagc tgatcgcccg cgagtacgac atcagcccgc gcaccatcga
     4321 ggtgtatcgg gccaacgtca tgaccaagat gcaggccaac agcctttcgg agctggttcg
     4381 cctcgcgatg cgcgccggca tgctcaacga ttgacaattg atgtaagtta gctcactcat
     4441 taggcaccgg gatctcgacc gatgcccttg agagccttca acccagtcag ctccttccgg
     4501 tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc
     4561 gtaggacagg tgccggcagc gctctgggtc attttcggcg aggaccgctt tcgctggagc
     4621 gcgacgatga tcggcctgtc gcttgcggta ttcggaatct tgcacgccct cgctcaagcc
     4681 ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc aggccattat cgccggcatg
     4741 gcggccccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
     4801 ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
     4861 tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
     4921 taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
     4981 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
     5041 ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
     5101 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
     5161 ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc
     5221 aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     5281 ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     5341 gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     5401 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     5461 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     5521 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     5581 tactgagagt gcaccatata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     5641 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     5701 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     5761 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     5821 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     5881 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     5941 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6001 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6061 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6121 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     6181 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     6241 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     6301 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     6361 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     6421 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     6481 ttaagggatt ttggtcatga acaataaaac tgtctgctta cataaacagt aatacaaggg
     6541 gtgttatgag ccatattcaa cgggaaacgt cttgctctag gccgcgatta aattccaaca
     6601 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
     6661 caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag
     6721 gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta
     6781 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca
     6841 ctgcgatccc cgggaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
     6901 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
     6961 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg
     7021 gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct
     7081 ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt
     7141 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac
     7201 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
     7261 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
     7321 ataaattgca gtttcatttg atgctcgatg agtttttcta agaattaatt catgagcgga
     7381 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     7441 aaagtgccac ctgaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt
     7501 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     7561 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     7621 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     7681 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     7741 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     7801 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     7861 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca
//
