LOCUS       Exported                8844 bp ds-DNA     circular UNA 21-JUN-2023
DEFINITION  Prime editing in mammalian cells.
ACCESSION   urn.local...g-eo7ib91
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 11, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     ##Imported_From-START##
            Path     :: /Users/kevinzhao/Library/Containers/com.tencent.xinWeCh 
                       at/Data/Library/Application
                        Support/com.tencent.xinWeChat/2.0b4.0.9/cfeb87444131905 
                       8c9b9cfeb41de5146/Message/MessageTemp/4c785ba1d498e60f3  
                      290ec98a0d64671/File
            Filename :: PE2.dna
            ##Imported_From-END##
FEATURES             Location/Qualifiers
     source          1..8844
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     regulatory      1..380
                     /standard_name="CMV enhancer"
                     /label=CMV enhancer
                     /note="Geneious type: enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      381..584
                     /standard_name="CMV promoter"
                     /label=CMV promoter
                     /note="Geneious type: promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     primer_bind     595..608
                     /label=LbCas12a-seqF1
     regulatory      626..644
                     /standard_name="T7 promoter"
                     /label=T7 promoter
                     /note="Geneious type: promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             658..714
                     /codon_start=1
                     /standard_name="BPNLS"
                     /label=BPNLS
                     /translation="MKRTADGSEFESPKKKRKV"
     CDS             721..4389
                     /codon_start=1
                     /standard_name="LbCpf1"
                     /label=rnCas12a-D156R-H759A-R1138A
                     /note="Geneious type: CDS"
                     /translation="EKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAEDYKG
                     VKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEIAKA
                     FKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFRNRENMFSEEAK
                     STSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGEFFN
                     FVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLSDRE
                     SLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNGPAI
                     STISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQLQEY
                     ADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDLLDS
                     VKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSKDKF
                     KLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGNYEK
                     INYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLIDFF
                     KDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVEEGK
                     LYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKEELV
                     VHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKINTEV
                     RVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDYHSL
                     LDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSGFKNS
                     RVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQNGFI
                     FYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDYKNF
                     SRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQGDI
                     RALLCEQSDKAFYSSFMALMSLMLQMANSITGRTDVDFLISPVKNSDGIFYDSRNYEAQ
                     ENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSVK"
     primer_bind     complement(735..751)
                     /label=CMV-seqR
     misc_feature    1174..1176
                     /label=D156R
     primer_bind     1269..1285
                     /label=LbCas12a-seqF2
     primer_bind     1985..2001
                     /label=LbCas12a-seqF3
     primer_bind     2739..2757
                     /label=LbCas12a-seqF4
     misc_feature    2953..2955
                     /label=H759A
     primer_bind     3470..3484
                     /label=LbCas12a-seqF5
     misc_feature    4120..4122
                     /label=R1138A
     primer_bind     4191..4206
                     /label=LbCas12a-seqF6
     CDS             4390..4485
                     /codon_start=1
                     /label=32aa Linker
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     CDS             4495..6036
                     /codon_start=1
                     /standard_name="MMLV"
                     /label=M-MLV RT?RNase H
                     /translation="TLNIEDEYRLHETSKEPDVSLGSTWLSDFPQAWAETGGMGLAVRQ
                     APLIIPLKATSTPVSIKQYPMSQEARLGIKPHIQRLLDQGILVPCQSPWNTPLLPVKKP
                     GTNDYRPVQDLREVNKRVEDIHPTVPNPYNLLSGLPPSHQWYTVLDLKDAFFCLRLHPT
                     SQPLFAFEWRDPEMGISGQLTWTRLPQGFKNSPTLFNEALHRDLADFRIQHPDLILLQY
                     VDDLLLAATSELDCQQGTRALLQTLGNLGYRASAKKAQICQKQVKYLGYLLKEGQRWLT
                     EARKETVMGQPTPKTPRQLREFLGKAGFCRLFIPGFAEMAAPLYPLTKPGTLFNWGPDQ
                     QKAYQEIKQALLTAPALGLPDLTKPFELFVDEKQGYAKGVLTQKLGPWRRPVAYLSKKL
                     DPVAAGWPPCLRMVAAIAVLTKDAGKLTMGQPLVILAPHAVEALVKQPPDRWLSNARMT
                     HYQALLLDTDRVQFGPVVALNPATLLPLPEEGLQHNCLDILAEAHGTRPDLTDQPL"
     primer_bind     4926..4941
                     /label=MMLV-seqF1
     primer_bind     5664..5679
                     /label=MMLV-seqF2
     CDS             6049..6099
                     /standard_name="BPNLS"
                     /label=BPNLS
     CDS             6108..6125
                     /codon_start=1
                     /standard_name="6xHis"
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    6154..6378
                     /standard_name="bGH poly(A) signal"
                     /label=bGH poly(A) signal
                     /note="Geneious type: polyA_signal"
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    6473..6489
                     /standard_name="lac operator"
                     /label=lac operator binding site
                     /bound_moiety="lac repressor encoded by
                     lacI"
                     /note="The lac repressor binds to the lac
                     operator to inhibit transcription in E. coli. This 
                     inhibition can be relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      complement(join(6522..6527,6504..6521,6497..6503))
                     /standard_name="lac promoter"
                     /label=lac promoter
                     /note="Geneious type: promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    6542..6563
                     /standard_name="CAP binding site"
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator
                     protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     primer_bind     6556..6574
                     /label=polyA-seqF
     rep_origin      complement(6851..7439)
                     /direction=LEFT
     primer_bind     7276..7294
                     /label=ori-seqF
     primer_bind     7997..8015
                     /label=Amp-seqF
     CDS             complement(join(8402..8470,7610..8401))
                     /codon_start=1
                     /gene="bla"
                     /standard_name="AmpR"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMS
                     TFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITM
                     SDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMAT
                     TLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIA
                     ALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*MSIQHFRVALIPFFAAF
                     CLPVFA"
     regulatory      complement(8471..8575)
                     /gene="bla"
                     /standard_name="AmpR promoter"
                     /label=AmpR promoter
                     /note="Geneious type: promoter"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      601 agatccgcta gagatccgcg gccgctaata cgactcacta tagggagagc cgccaccatg
      661 aaacggacag ccgacggaag cgagttcgag tcaccaaaga agaagcggaa agtcaagctg
      721 gagaagttta caaactgcta ctccctgtct aagaccctga ggttcaaggc catccctgtg
      781 ggcaagaccc aggagaacat cgacaataag cggctgctgg tggaggacga gaagagagcc
      841 gaggattata agggcgtgaa gaagctgctg gatcgctact atctgtcttt tatcaacgac
      901 gtgctgcaca gcatcaagct gaagaatctg aacaattaca tcagcctgtt ccggaagaaa
      961 accagaaccg agaaggagaa taaggagctg gagaacctgg agatcaatct gcggaaggag
     1021 atcgccaagg ccttcaaggg caacgagggc tacaagtccc tgtttaagaa ggatatcatc
     1081 gagacaatcc tgccagagtt cctggacgat aaggacgaga tcgccctggt gaacagcttc
     1141 aatggcttta ccacagcctt caccggcttc tttcgtaaca gagagaatat gttttccgag
     1201 gaggccaaga gcacatccat cgccttcagg tgtatcaacg agaatctgac ccgctacatc
     1261 tctaatatgg acatcttcga gaaggtggac gccatctttg ataagcacga ggtgcaggag
     1321 atcaaggaga agatcctgaa cagcgactat gatgtggagg atttctttga gggcgagttc
     1381 tttaactttg tgctgacaca ggagggcatc gacgtgtata acgccatcat cggcggcttc
     1441 gtgaccgaga gcggcgagaa gatcaagggc ctgaacgagt acatcaacct gtataatcag
     1501 aaaaccaagc agaagctgcc taagtttaag ccactgtata agcaggtgct gagcgatcgg
     1561 gagtctctga gcttctacgg cgagggctat acatccgatg aggaggtgct ggaggtgttt
     1621 agaaacaccc tgaacaagaa cagcgagatc ttcagctcca tcaagaagct ggagaagctg
     1681 ttcaagaatt ttgacgagta ctctagcgcc ggcatctttg tgaagaacgg ccccgccatc
     1741 agcacaatct ccaaggatat cttcggcgag tggaacgtga tccgggacaa gtggaatgcc
     1801 gagtatgacg atatccacct gaagaagaag gccgtggtga ccgagaagta cgaggacgat
     1861 cggagaaagt ccttcaagaa gatcggctcc ttttctctgg agcagctgca ggagtacgcc
     1921 gacgccgatc tgtctgtggt ggagaagctg aaggagatca tcatccagaa ggtggatgag
     1981 atctacaagg tgtatggctc ctctgagaag ctgttcgacg ccgattttgt gctggagaag
     2041 agcctgaaga agaacgacgc cgtggtggcc atcatgaagg acctgctgga ttctgtgaag
     2101 agcttcgaga attacatcaa ggccttcttt ggcgagggca aggagacaaa cagggacgag
     2161 tccttctatg gcgattttgt gctggcctac gacatcctgc tgaaggtgga ccacatctac
     2221 gatgccatcc gcaattatgt gacccagaag ccctactcta aggataagtt caagctgtat
     2281 tttcagaacc ctcagttcat gggcggctgg gacaaggata aggagacaga ctatcgggcc
     2341 accatcctga gatacggctc caagtactat ctggccatca tggataagaa gtacgccaag
     2401 tgcctgcaga agatcgacaa ggacgatgtg aacggcaatt acgagaagat caactataag
     2461 ctgctgcccg gccctaataa gatgctgcca aaggtgttct tttctaagaa gtggatggcc
     2521 tactataacc ccagcgagga catccagaag atctacaaga atggcacatt caagaagggc
     2581 gatatgttta acctgaatga ctgtcacaag ctgatcgact tctttaagga tagcatctcc
     2641 cggtatccaa agtggtccaa tgcctacgat ttcaactttt ctgagacaga gaagtataag
     2701 gacatcgccg gcttttacag agaggtggag gagcagggct ataaggtgag cttcgagtct
     2761 gccagcaaga aggaggtgga taagctggtg gaggagggca agctgtatat gttccagatc
     2821 tataacaagg acttttccga taagtctcac ggcacaccca atctgcacac catgtacttc
     2881 aagctgctgt ttgacgagaa caatcacgga cagatcaggc tgagcggagg agcagagctg
     2941 ttcatgaggc gcgcctccct gaagaaggag gagctggtgg tgcacccagc caactcccct
     3001 atcgccaaca agaatccaga taatcccaag aaaaccacaa ccctgtccta cgacgtgtat
     3061 aaggataaga ggttttctga ggaccagtac gagctgcaca tcccaatcgc catcaataag
     3121 tgccccaaga acatcttcaa gatcaataca gaggtgcgcg tgctgctgaa gcacgacgat
     3181 aacccctatg tgatcggcat cgacaggggc gagcgcaatc tgctgtatat cgtggtggtg
     3241 gacggcaagg gcaacatcgt ggagcagtat tccctgaacg agatcatcaa caacttcaac
     3301 ggcatcagga tcaagacaga ttaccactct ctgctggaca agaaggagaa ggagaggttc
     3361 gaggcccgcc agaactggac ctccatcgag aatatcaagg agctgaaggc cggctatatc
     3421 tctcaggtgg tgcacaagat ctgcgagctg gtggagaagt acgatgccgt gatcgccctg
     3481 gaggacctga actctggctt taagaatagc cgcgtgaagg tggagaagca ggtgtatcag
     3541 aagttcgaga agatgctgat cgataagctg aactacatgg tggacaagaa gtctaatcct
     3601 tgtgcaacag gcggcgccct gaagggctat cagatcacca ataagttcga gagctttaag
     3661 tccatgtcta cccagaacgg cttcatcttt tacatccctg cctggctgac atccaagatc
     3721 gatccatcta ccggctttgt gaacctgctg aaaaccaagt ataccagcat cgccgattcc
     3781 aagaagttca tcagctcctt tgacaggatc atgtacgtgc ccgaggagga tctgttcgag
     3841 tttgccctgg actataagaa cttctctcgc acagacgccg attacatcaa gaagtggaag
     3901 ctgtactcct acggcaaccg gatcagaatc ttccggaatc ctaagaagaa caacgtgttc
     3961 gactgggagg aggtgtgcct gaccagcgcc tataaggagc tgttcaacaa gtacggcatc
     4021 aattatcagc agggcgatat cagagccctg ctgtgcgagc agtccgacaa ggccttctac
     4081 tctagcttta tggccctgat gagcctgatg ctgcagatgg cgaacagcat cacaggccgc
     4141 accgacgtgg attttctgat cagccctgtg aagaactccg acggcatctt ctacgatagc
     4201 cggaactatg aggcccagga gaatgccatc ctgccaaaga acgccgacgc caatggcgcc
     4261 tataacatcg ccagaaaggt gctgtgggcc atcggccagt tcaagaaggc cgaggacgag
     4321 aagctggata aggtgaagat cgccatctct aacaaggagt ggctggagta cgcccagacc
     4381 agcgtgaagt ctggaggatc tagcggagga tcctctggca gcgagacacc aggaacaagc
     4441 gagtcagcaa caccagagag cagtggcggt tcgtctggag gttcacccgg gagcacccta
     4501 aatatagaag atgagtatcg gctacatgag acctcaaaag agccagatgt ttctctaggg
     4561 tccacatggc tgtctgattt tcctcaggcc tgggcggaaa ccgggggcat gggactggca
     4621 gttcgccaag ctcctctgat catacctctg aaagcaacct ctacccccgt gtccataaaa
     4681 caatacccca tgtcacaaga agccagactg gggatcaagc cccacataca gagactgttg
     4741 gaccagggaa tactggtacc ctgccagtcc ccctggaaca cgcccctgct acccgttaag
     4801 aaaccaggga ctaatgatta taggcctgtc caggatctga gagaagtcaa caagcgggtg
     4861 gaagacatcc accccaccgt gcccaaccct tacaacctct tgagcgggct cccaccgtcc
     4921 caccagtggt acactgtgct tgatttaaag gatgcctttt tctgcctgag actccacccc
     4981 accagtcagc ctctcttcgc ctttgagtgg agagatccag agatgggaat ctcaggacaa
     5041 ttgacctgga ccagactccc acagggtttc aaaaacagtc ccaccctgtt taatgaggca
     5101 ctgcacagag acctagcaga cttccggatc cagcacccag acttgatcct gctacagtac
     5161 gtggatgact tactgctggc cgccacttct gagctagact gccaacaagg tactcgggcc
     5221 ctgttacaaa ccctagggaa cctcgggtat cgggcctcgg ccaagaaagc ccaaatttgc
     5281 cagaaacagg tcaagtatct ggggtatctt ctaaaagagg gtcagagatg gctgactgag
     5341 gccagaaaag agactgtgat ggggcagcct actccgaaga cccctcgaca actaagggag
     5401 ttcctaggga aggcaggctt ctgtcgcctc ttcatccctg ggtttgcaga aatggcagcc
     5461 cccctgtacc ctctcaccaa accggggact ctgtttaatt ggggcccaga ccaacaaaag
     5521 gcctatcaag aaatcaagca agctcttcta actgccccag ccctggggtt gccagatttg
     5581 actaagccct ttgaactctt tgtcgacgag aagcagggct acgccaaagg tgtcctaacg
     5641 caaaaactgg gaccttggcg tcggccggtg gcctacctgt ccaaaaagct agacccagta
     5701 gcagctgggt ggcccccttg cctacggatg gtagcagcca ttgccgtact gacaaaggat
     5761 gcaggcaagc taaccatggg acagccacta gtcattctgg ccccccatgc agtagaggca
     5821 ctagtcaaac aaccccccga ccgctggctt tccaacgccc ggatgactca ctatcaggcc
     5881 ttgcttttgg acacggaccg ggtccagttc ggaccggtgg tagccctgaa cccggctacg
     5941 ctgctcccac tgcctgagga agggctgcaa cacaactgcc ttgatatcct ggccgaagcc
     6001 cacggaaccc gacccgacct aacggaccag ccgctctctg gcggctcaaa aagaaccgcc
     6061 gacggcagcg aattcgagcc caagaagaag aggaaagtct aaccggtcat catcaccatc
     6121 accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     6181 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     6241 cctaataaaa tgagaaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     6301 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     6361 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg ataccgtcga
     6421 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     6481 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tagggtgcct
     6541 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     6601 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     6661 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     6721 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     6781 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     6841 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     6901 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     6961 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7021 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7081 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7141 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7201 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7261 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     7321 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7381 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7441 aagatccttt gatcttttct acggggtctg acactcagtg gaacgaaaac tcacgttaag
     7501 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     7561 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     7621 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     7681 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     7741 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     7801 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     7861 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     7921 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     7981 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     8041 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     8101 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     8161 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     8221 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     8281 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     8341 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     8401 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     8461 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     8521 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     8581 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcgatctccc gatcccctag
     8641 ggtctactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     8701 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
     8761 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
     8821 atgtacgggc cagatatacg cgtt
//
