LOCUS       Exported                7666 bp ds-DNA     circular SYN 02-OCT-2020
DEFINITION  synthetic circular DNA
ACCESSION   VB190607-1060zej
VERSION     .
KEYWORDS    VB190607-1060zej.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported to SnapGene on Jun 07, 2019
REFERENCE   2  (bases 1 to 7666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 11, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7666
                     /organism="synthetic DNA construct"
     misc_feature    1..313
                     /label=5' ITR
                     /label=nonstandard type:  ITR
                     /note="/application_notes=Recognized by PBase transposase; 
                     DNA
                     flanked by piggyBac 5' ITR and 3' ITR can be transposed by 
                     PBase into TTAA sites."
                     /note="/description=piggyBac 5' inverted terminal repeat"
                     /note="/official_designation=5' ITR"
                     /note="/uuid=cf6f8504beb5412ca461e47810e15037"
     promoter        337..1166
                     /label={MGT#1}
                     /note="/application_notes="
                     /note="/official_designation={MGT#1}"
                     /note="/uuid=00427d60c55740498785d056bde4b49d"
     misc_feature    1191..1196
                     /label=Kozak
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Facilitates translation 
                     initiation of
                     ATG start codon downstream of the Kozak sequence."
                     /note="/description=Kozak translation initiation sequence"
                     /note="/full_name=Kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=5f18b25a1234443494a618363f09f052"
     misc_feature    1197..2042
                     /label=d2EGFP
                     /label=nonstandard type:  ORF
                     /note="/application_notes=Fast turnover (fluorescence half 
                     life
                     is 2 hours)."
                     /note="/description=Destabilized EGFP due to addition of 
                     PEST
                     sequence from mouse Odc1 gene"
                     /note="/official_designation=d2EGFP"
                     /note="/uuid=23042ceceb6141d0a84c78807f35bd0d"
     polyA_signal    2070..2591
                     /label=rBG pA
                     /note="/application_notes=Allows transcription termination 
                     and
                     polyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/description=Rabbit beta-globin polyadenylation 
                     signal"
                     /note="/full_name=Rabbit beta-globin polyadenylation 
                     signal"
                     /note="/official_designation=rBG pA"
                     /note="/uuid=8fa32026eca4441f81c85dc922b69b4a"
     promoter        2617..3204
                     /label=CMV promoter
                     /note="/application_notes=Strong promoter; may have 
                     variable
                     strength in some cell types."
                     /note="/description=Human cytomegalovirus immediate early
                     enhancer/promoter"
                     /note="/full_name=Human cytomegalovirus immediate early 
                     promoter"
                     /note="/official_designation=CMV"
                     /note="/uuid=5257af780b9a4261b6ff271ec041eff9"
     misc_feature    3236..3946
                     /label=mCherry
                     /label=nonstandard type:  ORF
                     /note="/application_notes=Commonly used red fluorescent 
                     protein;
                     fast maturation compared to its predecessor, mRFP1."
                     /note="/description=Variant of mRFP1 generated by 
                     mutagenesis"
                     /note="/full_name=mCherry fluorescent protein"
                     /note="/official_designation=mCherry"
                     /note="/uuid=d89ccb82851e44c6adf82649b5a9d162"
     polyA_signal    3990..4214
                     /label=BGH pA
                     /note="/application_notes=Allows transcription termination 
                     and
                     polyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/description=Bovine growth hormone polyadenylation 
                     signal"
                     /note="/full_name=Bovine growth hormone polyadenylation"
                     /note="/official_designation=BGH pA"
                     /note="/uuid=4dab41530ef84813b9bdcb81afeec302"
     misc_feature    complement(4396..4630)
                     /label=3' ITR
                     /label=nonstandard type:  ITR
                     /note="/application_notes=Recognized by PBase transposase; 
                     DNA
                     flanked by piggyBac 5' ITR and 3' ITR can be transposed by 
                     PBase into TTAA sites."
                     /note="/description=piggyBac 3' inverted terminal repeat"
                     /note="/official_designation=3' ITR"
                     /note="/uuid=22382df36c4b4754b7821e5d7876f273"
     misc_feature    5462..6322
                     /label=Ampicillin
                     /label=nonstandard type:  ORF
                     /note="/application_notes=Allows E. coli to be resistant to
                     ampicillin."
                     /note="/description=Ampicillin resistance gene"
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"
     rep_origin      6493..7081
                     /direction=RIGHT
                     /label=pUC ori
                     /note="/application_notes=Facilitates plasmid replication 
                     in E.
                     coli; regulates high-copy plasmid number (500-700)."
                     /note="/description=pUC origin of replication"
                     /note="/full_name=pUC origin of replication"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=b978ad4ff785460db448caf63b728237"
ORIGIN
        1 ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc
       61 tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga
      121 gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc
      181 gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg
      241 ataattatat tgttatttca tgttctactt acgtgataac ttattatata tatattttct
      301 tgttatagat atcatcaact ttgtatagaa aagttgatat ttatttttag gaccagaaag
      361 ttaaagtgaa ttggatttga tccattttct gaaaggctgg caagaattct tgacattgca
      421 caggaatttc catgtcagca tgttctcaca tgtatgatct aatttaggtg acacttcctg
      481 gctcaggaag ttgaatttca ttaagccttt gtggtttggg gctctgctgt gctttgacag
      541 ctctgatctc ctcccttccg gctgggctgt ctggggcgct ctaaaatgag tgttgattta
      601 atgcactgcc ttcgcacccg tgctggtaga ttattttggg gggcgggggt tgaggaaatg
      661 gcatgactca gagtttaaaa gccccaaatc ttagctgtgc ctgtgtagct ttaccacata
      721 acccattgat aacttagttg tgcaaccatc accaccatct gttttcagaa ctcttttcat
      781 tttgcgaaac tgaaacccgt taagcactga tttcccactc tccctcctcc cagcccatag
      841 caaaccacca tcccaccagc actttcattt cgcaaatggc aaaactgaag ccgatattgt
      901 ggttgtgact tatcccaaag taatatacac ataaacctct atggatgagg aaaaagacag
      961 agggaaacta aaaattcaaa agaacaaatt tgactcacag atttgctgac tcatagtttc
     1021 gctttcgctt ttgctttggc ccccgagggc tcggagaggt gcaaagcggt gacagatccg
     1081 tgactcagcg tccccacccc tgctggggaa ggagccccgc cggccgccgc ggcaccgcac
     1141 agccacccct gtccccggga cgcctacaag tttgtacaaa aaagcaggct gccaccatgg
     1201 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     1261 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     1321 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     1381 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     1441 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     1501 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     1561 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     1621 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     1681 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     1741 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     1801 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     1861 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagaagctta
     1921 gccatggctt cccgccggag gtggaggagc aggatgatgg cacgctgccc atgtcttgtg
     1981 cccaggagag cgggatggac cgtcaccctg cagcctgtgc ttctgctagg atcaatgtgt
     2041 agacccagct ttcttgtaca aagtggtgat cctcaggtgc aggctgccta tcagaaggtg
     2101 gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc
     2161 aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa
     2221 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat
     2281 gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat
     2341 gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa
     2401 cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt
     2461 tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt
     2521 ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc
     2581 ttatggagat ccctcgacct gcagcccaag cttcgcgttg acattgatta ttgactagtt
     2641 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     2701 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     2761 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     2821 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     2881 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     2941 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     3001 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     3061 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     3121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     3181 gggaggtcta tataagcaga gctctctggc taactagaga acccactgcg ccaccatggt
     3241 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     3301 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     3361 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     3421 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     3481 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     3541 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     3601 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     3661 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     3721 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     3781 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     3841 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     3901 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaactcg agtctagagg
     3961 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     4021 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     4081 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     4141 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     4201 ggtgggctct atggctcgag ttaattaacg agagcataat attgatatgt gccaaagttg
     4261 tttctgactg actaataagt ataatttgtt tctattatgt ataggttaag ctaattactt
     4321 attttataat acaacatgac tgtttttaaa gtacaaaata agtttatttt tgtaaaagag
     4381 agaatgttta aaagttttgt tactttatag aagaaatttt gagtttttgt ttttttttaa
     4441 taaataaata aacataaata aattgtttgt tgaatttatt attagtatgt aagtgtaaat
     4501 ataataaaac ttaatatcta ttcaaattaa taaataaacc tcgatataca gaccgataaa
     4561 acacatgcgt caattttacg catgattatc tttaacgtac gtcacaatat gattatcttt
     4621 ctagggttaa ataatagttt ctaatttttt tattattcag cctgctgtcg tgaataccga
     4681 gctccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc
     4741 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     4801 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     4861 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     4921 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     4981 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     5041 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     5101 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     5161 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     5221 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     5281 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     5341 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     5401 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     5461 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     5521 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     5581 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     5641 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     5701 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     5761 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     5821 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     5881 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     5941 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     6001 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     6061 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     6121 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     6181 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     6241 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     6301 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     6361 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     6421 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     6481 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     6541 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     6601 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     6661 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     6721 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     6781 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6841 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6901 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6961 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     7021 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     7081 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     7141 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     7201 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     7261 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     7321 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     7381 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     7441 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct
     7501 cgaaattaac cctcactaaa gggaacaaaa gctggtacct cgcgcgactt ggtttgccat
     7561 tctttagcgc gcgtcgcgtc acacagcttg gccacaatgt ggtttttgtc aaacgaagat
     7621 tctatgacgt gtttaaagtt taggtcgagt aaagcgcaaa tctttt
//
