LOCUS       Exported                7896 bp ds-DNA     circular SYN 02-JUN-2021
DEFINITION  synthetic circular DNA
ACCESSION   VB190606-1098kkx
VERSION     .
KEYWORDS    VB190606-1098kkx.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 11, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7896
                     /organism="synthetic DNA construct"
     misc_feature    1..313
                     /label=5' ITR
                     /label=nonstandard type:  ITR
                     /note="/application_notes=Recognized by PBase transposase; 
                     DNA
                     flanked by piggyBac 5' ITR and 3' ITR can be transposed by 
                     PBase into TTAA sites."
                     /note="/description=piggyBac 5' inverted terminal repeat"
                     /note="/official_designation=5' ITR"
                     /note="/uuid=cf6f8504beb5412ca461e47810e15037"
     promoter        337..1396
                     /label={CLGT#3}
                     /note="/application_notes="
                     /note="/official_designation={CLGT#3}"
                     /note="/uuid=29b0d810859a494aba2afdd0caaf382b"
     misc_feature    1421..1426
                     /label=Kozak
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Facilitates translation 
                     initiation of
                     ATG start codon downstream of the Kozak sequence."
                     /note="/description=Kozak translation initiation sequence"
                     /note="/full_name=Kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=5f18b25a1234443494a618363f09f052"
     misc_feature    1427..2272
                     /label=d2EGFP
                     /label=nonstandard type:  ORF
                     /note="/application_notes=Fast turnover (fluorescence half 
                     life
                     is 2 hours)."
                     /note="/description=Destabilized EGFP due to addition of 
                     PEST
                     sequence from mouse Odc1 gene"
                     /note="/official_designation=d2EGFP"
                     /note="/uuid=23042ceceb6141d0a84c78807f35bd0d"
     polyA_signal    2300..2821
                     /label=rBG pA
                     /note="/application_notes=Allows transcription termination 
                     and
                     polyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/description=Rabbit beta-globin polyadenylation 
                     signal"
                     /note="/full_name=Rabbit beta-globin polyadenylation 
                     signal"
                     /note="/official_designation=rBG pA"
                     /note="/uuid=8fa32026eca4441f81c85dc922b69b4a"
     promoter        2847..3434
                     /label=CMV promoter
                     /note="/application_notes=Strong promoter; may have 
                     variable
                     strength in some cell types."
                     /note="/description=Human cytomegalovirus immediate early
                     enhancer/promoter"
                     /note="/full_name=Human cytomegalovirus immediate early 
                     promoter"
                     /note="/official_designation=CMV"
                     /note="/uuid=5257af780b9a4261b6ff271ec041eff9"
     misc_feature    3466..4176
                     /label=mCherry
                     /label=nonstandard type:  ORF
                     /note="/application_notes=Commonly used red fluorescent 
                     protein;
                     fast maturation compared to its predecessor, mRFP1."
                     /note="/description=Variant of mRFP1 generated by 
                     mutagenesis"
                     /note="/full_name=mCherry fluorescent protein"
                     /note="/official_designation=mCherry"
                     /note="/uuid=d89ccb82851e44c6adf82649b5a9d162"
     polyA_signal    4220..4444
                     /label=BGH pA
                     /note="/application_notes=Allows transcription termination 
                     and
                     polyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/description=Bovine growth hormone polyadenylation 
                     signal"
                     /note="/full_name=Bovine growth hormone polyadenylation"
                     /note="/official_designation=BGH pA"
                     /note="/uuid=4dab41530ef84813b9bdcb81afeec302"
     misc_feature    complement(4626..4860)
                     /label=3' ITR
                     /label=nonstandard type:  ITR
                     /note="/application_notes=Recognized by PBase transposase; 
                     DNA
                     flanked by piggyBac 5' ITR and 3' ITR can be transposed by 
                     PBase into TTAA sites."
                     /note="/description=piggyBac 3' inverted terminal repeat"
                     /note="/official_designation=3' ITR"
                     /note="/uuid=22382df36c4b4754b7821e5d7876f273"
     misc_feature    5692..6552
                     /label=Ampicillin
                     /label=nonstandard type:  ORF
                     /note="/application_notes=Allows E. coli to be resistant to
                     ampicillin."
                     /note="/description=Ampicillin resistance gene"
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"
     rep_origin      6723..7311
                     /direction=RIGHT
                     /label=pUC ori
                     /note="/application_notes=Facilitates plasmid replication 
                     in E.
                     coli; regulates high-copy plasmid number (500-700)."
                     /note="/description=pUC origin of replication"
                     /note="/full_name=pUC origin of replication"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=b978ad4ff785460db448caf63b728237"
ORIGIN
        1 ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc
       61 tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga
      121 gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc
      181 gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg
      241 ataattatat tgttatttca tgttctactt acgtgataac ttattatata tatattttct
      301 tgttatagat atcatcaact ttgtatagaa aagttgtccc cctggccact ctgctcaaac
      361 gccccaccct gttctctctg tccccttccc ctgcttaatt tttcttggaa ccacttgtca
      421 ttcctgtcat tgttccacct atgcgtttgg tttcttgttt tctttttttg gggtggagtc
      481 ttgctctgga aagaattttc ttttaattct ccacttgtaa ataacacgtg tcatcagaat
      541 agttaattag gcctgggcat ggtggtgtag tcccagctct ttgggaggcc gaggtcagcg
      601 gattacaagg tcaggtgatc aagaccatcc tggccaacaa acactgtaat tattaaataa
      661 tataattatt tacatataat taaattatac aattattaaa tataattatg tatatgtaat
      721 taaattatat aattattaga taattgttta tatataatta aattattata taataaatta
      781 tataatagat gtttacctgt ccagccccag gtgggctcga cccacaggtc agaggtcatg
      841 agaagctgga tgagaccact ggggatgtcc ctgttttctc agtatatcag ccaaggacct
      901 gaagaacatg ctgtcccagg tcaactaccg ggtccccaac ctcctgacgt caggtgatcc
      961 gcctgcctcg gccccccaaa atgctgggat taaaggcgtg agccacctcg cccagcctct
     1021 ggaattttcc atttaatatt tttggaccaa agttgaccaa ggataactga aaccgaggaa
     1081 atcgaaactg ctgtgaaata gtactaatga agtcaagcaa gtatgacact catactttgt
     1141 tgatggtcat aaaaaataga cagccacatt agataataat atagtaggct tgaccgggcg
     1201 tggtggctca cacctgtaat cccagcactt tgggaggcca agctgctgcc tcgccccgcc
     1261 tcacgccggc gtcctggggg cgcggagagg gcgcccaggt tcaccgcgct ctcagcgcgc
     1321 acttccgggc acggtcaggg cgtgacgtcg ggaggagggc aggtaagtgc cggcgggcgg
     1381 aggagcgggg aggctacaag tttgtacaaa aaagcaggct gccaccatgg tgagcaaggg
     1441 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     1501 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     1561 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     1621 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     1681 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     1741 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     1801 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     1861 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     1921 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     1981 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     2041 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     2101 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagaagctta gccatggctt
     2161 cccgccggag gtggaggagc aggatgatgg cacgctgccc atgtcttgtg cccaggagag
     2221 cgggatggac cgtcaccctg cagcctgtgc ttctgctagg atcaatgtgt agacccagct
     2281 ttcttgtaca aagtggtgat cctcaggtgc aggctgccta tcagaaggtg gtggctggtg
     2341 tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg
     2401 gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca
     2461 ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa
     2521 atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc
     2581 tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg
     2641 ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt
     2701 gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca
     2761 gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat
     2821 ccctcgacct gcagcccaag cttcgcgttg acattgatta ttgactagtt attaatagta
     2881 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     2941 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     3001 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     3061 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     3121 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     3181 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     3241 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     3301 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     3361 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     3421 tataagcaga gctctctggc taactagaga acccactgcg ccaccatggt gagcaagggc
     3481 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
     3541 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     3601 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     3661 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     3721 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     3781 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     3841 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
     3901 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     3961 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     4021 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     4081 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     4141 cactccaccg gcggcatgga cgagctgtac aagtaactcg agtctagagg gcccgtttaa
     4201 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     4261 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     4321 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     4381 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     4441 atggctcgag ttaattaacg agagcataat attgatatgt gccaaagttg tttctgactg
     4501 actaataagt ataatttgtt tctattatgt ataggttaag ctaattactt attttataat
     4561 acaacatgac tgtttttaaa gtacaaaata agtttatttt tgtaaaagag agaatgttta
     4621 aaagttttgt tactttatag aagaaatttt gagtttttgt ttttttttaa taaataaata
     4681 aacataaata aattgtttgt tgaatttatt attagtatgt aagtgtaaat ataataaaac
     4741 ttaatatcta ttcaaattaa taaataaacc tcgatataca gaccgataaa acacatgcgt
     4801 caattttacg catgattatc tttaacgtac gtcacaatat gattatcttt ctagggttaa
     4861 ataatagttt ctaatttttt tattattcag cctgctgtcg tgaataccga gctccaattc
     4921 gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga
     4981 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     5041 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     5101 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     5161 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     5221 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     5281 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     5341 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     5401 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     5461 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     5521 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     5581 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5641 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     5701 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     5761 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     5821 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     5881 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     5941 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     6001 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     6061 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     6121 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     6181 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     6241 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     6301 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     6361 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     6421 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     6481 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     6541 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     6601 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     6661 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6721 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6781 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6841 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     6901 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6961 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     7021 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     7081 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     7141 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7201 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7261 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7321 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     7381 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     7441 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     7501 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     7561 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     7621 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     7681 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct cgaaattaac
     7741 cctcactaaa gggaacaaaa gctggtacct cgcgcgactt ggtttgccat tctttagcgc
     7801 gcgtcgcgtc acacagcttg gccacaatgt ggtttttgtc aaacgaagat tctatgacgt
     7861 gtttaaagtt taggtcgagt aaagcgcaaa tctttt
//
